Project name: 8f047139587eaf1 [mutate: FB19D, WB23D]

Chain sequence(s)	B: LSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGP
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	FB19D, WB23D
Energy difference between WT (input) and mutated protein (by FoldX)	0.654785 kcal/mol

• Minimal score value: -3.3639
• Maximal score value: 1.8919
• Average score: -0.9431
• Total score value: -44.3263

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
14	L	B	0.8202
15	S	B	-0.6768
16	Q	B	-2.2372
17	E	B	-2.8039
18	T	B	-2.5808
19	D	B	-3.3450	mutated: FB19D
20	S	B	-2.6825
21	D	B	-3.0214
22	L	B	-1.1147
23	D	B	-1.8825	mutated: WB23D
24	K	B	-1.9755
25	L	B	0.5867
26	L	B	0.5367
27	P	B	-0.5542
28	E	B	-1.9145
29	N	B	-1.7346
30	N	B	-1.3614
31	V	B	0.8476
32	L	B	1.5765
33	S	B	0.7897
34	P	B	1.0768
35	L	B	1.8919
36	P	B	0.3060
37	S	B	-0.2263
38	Q	B	-1.1445
39	A	B	-0.5694
40	M	B	0.3266
41	D	B	-1.2005
42	D	B	-0.5692
43	L	B	0.9048
44	M	B	1.4231
45	L	B	0.3833
46	S	B	-0.9099
47	P	B	-2.1586
48	D	B	-3.3639
49	D	B	-3.0110
50	I	B	0.0000
51	E	B	-2.8148
52	Q	B	-2.2713
53	W	B	0.0556
54	F	B	0.7776
55	T	B	-0.9023
56	E	B	-2.6758
57	D	B	-2.9949
58	P	B	-1.7268
59	G	B	-1.3968
60	P	B	-0.8084