Project name: 1408d88f3af2cef [mutate: YA151A]

Chain sequence(s)	A: SKGEELFTGKVPILVELDGDVNGHKFSVSGEGEGDATKGKLTLKFICTTGKLPVPWPTLVTTLVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKEFVTAAGIT
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA151A
Energy difference between WT (input) and mutated protein (by FoldX)	1.16983 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -4.1986
• Maximal score value: 0.0348
• Average score: -1.0793
• Total score value: -243.9147

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
2	S	A	-1.9708
3	K	A	-2.4306
4	G	A	0.0000
5	E	A	-1.9836
6	E	A	-2.3940
7	L	A	-1.1917
8	F	A	0.0000
9	T	A	-1.1596
10	G	A	-1.5007
11	K	A	-2.2741
12	V	A	0.0000
13	P	A	-1.4475
14	I	A	0.0000
15	L	A	-1.0191
16	V	A	0.0000
17	E	A	-2.0040
18	L	A	0.0000
19	D	A	-3.4657
20	G	A	0.0000
21	D	A	-3.0120
22	V	A	0.0000
23	N	A	-2.1085
24	G	A	-1.7499
25	H	A	-2.2524
26	K	A	-3.0478
27	F	A	0.0000
28	S	A	-1.7885
29	V	A	0.0000
30	S	A	-1.0504
31	G	A	0.0000
32	E	A	-2.1661
33	G	A	-1.7787
34	E	A	-1.8450
35	G	A	0.0000
36	D	A	0.0000
37	A	A	0.0000
38	T	A	-1.6333
39	K	A	-2.2079
40	G	A	0.0000
41	K	A	-1.6481
42	L	A	0.0000
43	T	A	-1.1465
44	L	A	0.0000
45	K	A	-1.3842
46	F	A	0.0000
47	I	A	-1.1029
48	C	A	0.0000
49	T	A	-0.7688
50	T	A	-1.1754
51	G	A	-1.6879
52	K	A	-2.2877
53	L	A	0.0000
54	P	A	-1.2003
55	V	A	0.0000
56	P	A	0.0000
57	W	A	0.0000
58	P	A	0.0000
59	T	A	0.0000
60	L	A	0.0000
61	V	A	0.0000
62	T	A	-0.0039
63	T	A	0.0000
64	L	A	0.0000
68	V	A	-0.0846
69	Q	A	-0.1924
70	C	A	0.0000
71	F	A	0.0000
72	S	A	0.0000
73	R	A	-1.8001
74	Y	A	0.0000
75	P	A	-1.8505
76	D	A	-2.9078
77	H	A	-2.3774
78	M	A	0.0000
79	K	A	-2.7962
80	R	A	-2.7741
81	H	A	-1.7388
82	D	A	0.0000
83	F	A	0.0000
84	F	A	0.0000
85	K	A	0.0000
86	S	A	-1.1569
87	A	A	0.0000
88	M	A	0.0000
89	P	A	-1.5055
90	E	A	-1.9565
91	G	A	0.0000
92	Y	A	0.0000
93	V	A	-0.8649
94	Q	A	0.0000
95	E	A	-1.8936
96	R	A	0.0000
97	T	A	-0.9553
98	I	A	0.0000
99	S	A	-1.0373
100	F	A	0.0000
101	K	A	-2.1480
102	D	A	-2.8037
103	D	A	-2.6134
104	G	A	0.0000
105	N	A	-1.4773
106	Y	A	0.0000
107	K	A	-2.3927
108	T	A	0.0000
109	R	A	-3.4957
110	A	A	0.0000
111	E	A	-2.1822
112	V	A	0.0000
113	K	A	-1.3523
114	F	A	-1.4102
115	E	A	-1.6365
116	G	A	-1.7093
117	D	A	-2.1180
118	T	A	-1.5221
119	L	A	0.0000
120	V	A	0.0000
121	N	A	0.0000
122	R	A	-2.4240
123	I	A	0.0000
124	E	A	-4.1986
125	L	A	0.0000
126	K	A	-3.3099
127	G	A	0.0000
128	I	A	-1.2158
129	D	A	-2.2334
130	F	A	0.0000
131	K	A	-3.7754
132	E	A	-3.8000
133	D	A	-3.3488
134	G	A	-2.6361
135	N	A	-2.0776
136	I	A	0.0000
137	L	A	-1.6724
138	G	A	-1.8548
139	H	A	-1.4117
140	K	A	-1.9995
141	L	A	-1.5274
142	E	A	-1.8385
143	Y	A	-0.5038
144	N	A	-0.5553
145	Y	A	-0.8066
146	N	A	-1.2029
147	S	A	-1.2672
148	H	A	-1.5813
149	N	A	-1.5720
150	V	A	0.0000
151	A	A	-0.7558	mutated: YA151A
152	I	A	0.0000
153	T	A	-1.4964
154	A	A	-1.8813
155	D	A	-2.4877
156	K	A	-3.1375
157	Q	A	-3.1939
158	K	A	-3.4022
159	N	A	-2.4883
160	G	A	0.0000
161	I	A	0.0000
162	K	A	-1.4140
163	A	A	0.0000
164	N	A	-1.3337
165	F	A	0.0000
166	K	A	-1.9626
167	I	A	0.0000
168	R	A	-1.6984
169	H	A	0.0000
170	N	A	-1.4901
171	I	A	0.0000
172	E	A	-3.2372
173	D	A	-3.0282
174	G	A	-1.9497
175	S	A	-1.0847
176	V	A	-0.5251
177	Q	A	0.0000
178	L	A	-1.1591
179	A	A	0.0000
180	D	A	-1.1492
181	H	A	0.0000
182	Y	A	0.0348
183	Q	A	0.0000
184	Q	A	-1.4323
185	N	A	0.0000
186	T	A	-0.8707
187	P	A	-0.8630
188	I	A	-0.2536
189	G	A	-1.2175
190	D	A	-2.0953
191	G	A	-1.5113
192	P	A	-0.9896
193	V	A	-0.5629
194	L	A	-0.2847
195	L	A	-0.1769
196	P	A	0.0000
197	D	A	-2.4245
198	N	A	-1.9854
199	H	A	0.0000
200	Y	A	-0.3723
201	L	A	0.0000
202	S	A	-0.7325
203	T	A	-0.7587
204	Q	A	-1.4337
205	S	A	-0.9337
206	A	A	-0.5338
207	L	A	-0.4490
208	S	A	-0.9671
209	K	A	-1.8775
210	D	A	-2.1482
211	P	A	-1.8072
212	N	A	-2.4522
213	E	A	-2.6078
214	K	A	-3.0464
215	R	A	-3.1656
216	D	A	-2.1848
217	H	A	0.0000
218	M	A	0.0000
219	V	A	-0.9338
220	L	A	0.0000
221	K	A	-1.3488
222	E	A	-0.7117
223	F	A	-0.1384
224	V	A	0.0000
225	T	A	-0.7421
226	A	A	0.0000
227	A	A	-0.3946
228	G	A	-0.5726
229	I	A	-0.4561
230	T	A	-0.1842