Project name: obj1 [mutate: VR5C, ED6C, GE9C, GK10C, LT11C, LA45C, WH47C, YP59C, IG93C, YL95C, WD110C, GC113C, TV114C, LY115C, TS117C]

Chain sequence(s)	C: EVQLVESGGGLVQPGGSLRLSCAASDFTFRSYEMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARLRDGFNKGFDYWGQGTLVTVSS
Selected Chain(s)	C
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No
Mutated residues	IG93C,LA45C,WH47C,YL95C,LY115C,YP59C,GE9C,TS117C,TV114C,LT11C,GK10C,GC113C,ED6C,VR5C,WD110C
Energy difference between WT (input) and mutated protein (by FoldX)	33.7761 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with C chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       FoldX:    Building mutant model                                                       (00:00:25)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:02:02)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:02:31)
[INFO]       Main:     Simulation completed successfully.                                          (00:02:32)

• Minimal score value: -3.2274
• Maximal score value: 1.5423
• Average score: -0.9683
• Total score value: -116.1972

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	C	-2.0240
2	V	C	-1.2725
3	Q	C	-2.3991
4	L	C	0.0000
5	R	C	-2.6618	mutated: VR5C
6	D	C	0.0000	mutated: ED6C
7	S	C	-1.1443
8	G	C	-1.1564
9	E	C	-1.3444	mutated: GE9C
10	K	C	-1.6073	mutated: GK10C
11	T	C	-1.0387	mutated: LT11C
12	V	C	-1.2830
13	Q	C	-1.7255
14	P	C	-1.6780
15	G	C	-1.4188
16	G	C	-1.1986
17	S	C	-1.3266
18	L	C	0.0000
19	R	C	-2.2414
20	L	C	0.0000
21	S	C	-0.9613
22	C	C	0.0000
23	A	C	-1.2225
24	A	C	0.0000
25	S	C	-0.6185
26	D	C	0.0000
27	F	C	1.5423
28	T	C	0.2557
29	F	C	0.0000
30	R	C	-2.0140
31	S	C	-0.8631
32	Y	C	-1.1748
33	E	C	-1.0282
34	M	C	0.0000
35	S	C	0.0000
36	W	C	0.0000
37	V	C	0.0000
38	R	C	0.0000
39	Q	C	-1.4416
40	A	C	-1.5561
41	P	C	-1.4616
42	G	C	-1.6167
43	K	C	-2.5057
44	G	C	-1.7894
45	A	C	-1.3506	mutated: LA45C
46	E	C	-1.5572
47	H	C	-0.9286	mutated: WH47C
48	V	C	0.0000
49	S	C	0.0000
50	A	C	0.0000
51	I	C	0.0000
52	S	C	-0.6977
53	G	C	-1.2166
54	S	C	-1.2238
55	G	C	-1.0940
56	G	C	-0.7666
57	S	C	-0.5980
58	T	C	-0.3262
59	P	C	-0.4112	mutated: YP59C
60	Y	C	-0.9388
61	A	C	-1.5280
62	D	C	-2.4404
63	S	C	-1.7035
64	V	C	0.0000
65	K	C	-2.5087
66	G	C	-1.6087
67	R	C	0.0000
68	F	C	0.0000
69	T	C	-0.7799
70	I	C	0.0000
71	S	C	-0.5696
72	R	C	-1.3595
73	D	C	-1.9785
74	N	C	-2.1871
75	S	C	-1.7905
76	K	C	-2.3156
77	N	C	-1.6464
78	T	C	0.0000
79	L	C	0.0000
80	Y	C	-0.6336
81	L	C	0.0000
82	Q	C	-1.2815
83	M	C	0.0000
84	N	C	-1.3770
85	S	C	-1.2552
86	L	C	0.0000
87	R	C	-2.4879
88	A	C	-1.9143
89	E	C	-2.3534
90	D	C	0.0000
91	T	C	-1.0102
92	A	C	0.0000
93	G	C	-0.0335	mutated: IG93C
94	Y	C	0.0000
95	L	C	-0.6716	mutated: YL95C
96	C	C	0.0000
97	A	C	0.0000
98	R	C	0.0000
99	L	C	0.0000
100	R	C	-2.9887
101	D	C	-3.2274
102	G	C	-1.9775
103	F	C	-1.1951
104	N	C	-2.3198
105	K	C	-3.0530
106	G	C	-1.6421
107	F	C	-0.9356
108	D	C	-1.4115
109	Y	C	-1.2751
110	D	C	-2.1874	mutated: WD110C
111	G	C	0.0000
112	Q	C	-2.1287
113	C	C	-0.8029	mutated: GC113C
114	V	C	0.0000	mutated: TV114C
115	Y	C	0.6097	mutated: LY115C
116	V	C	0.0000
117	S	C	-0.9004	mutated: TS117C
118	V	C	0.0000
119	S	C	-1.1465
120	S	C	-1.0954