Project name: sabiking123

Chain sequence(s)	H: EVQLLESGGGLVQPGGSLRLSCAASDDTFSRYDMSWVRQAPGKGLEWVSAEDGSDDETYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARDHYDSGYYRMDEVWGQGTLVTVSS; L: DIQMTQSPSSLSASVGDRVTITCRASRSISSYLNWYQQKPGKAPKLLIYAEDSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCENWYDWPRTFGQGTKLEIK
Selected Chain(s)	H,L
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:09)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:09)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:09)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:09)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:09)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:03:57)
[INFO]       Main:     Simulation completed successfully.                                          (00:03:59)

• Minimal score value: -3.6442
• Maximal score value: 1.5866
• Average score: -0.7249
• Total score value: -166.0094

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
0	E	H	-2.8805
1	V	H	0.0000
2	Q	H	-2.0309
3	L	H	0.0000
4	L	H	0.8526
5	E	H	0.2947
6	S	H	-0.3175
7	G	H	-0.6086
8	G	H	0.1432
9	G	H	0.7300
10	L	H	1.2746
11	V	H	0.0000
12	Q	H	-1.4339
13	P	H	-1.6397
14	G	H	-1.4618
15	G	H	-1.0326
16	S	H	-1.3539
17	L	H	-1.1517
18	R	H	-2.2480
19	L	H	0.0000
20	S	H	-0.4948
21	C	H	0.0000
22	A	H	-0.2226
23	A	H	-1.2298
24	S	H	-2.1228
25	D	H	-3.3540
26	D	H	-3.1370
27	T	H	-1.8436
28	F	H	0.0000
29	S	H	-1.9091
30	R	H	-2.2009
31	Y	H	0.0000
32	D	H	0.0000
33	M	H	0.0000
34	S	H	0.0000
35	W	H	0.0000
36	V	H	0.0000
37	R	H	0.0000
38	Q	H	-0.7206
39	A	H	-1.1253
40	P	H	-0.8731
41	G	H	-1.5116
42	K	H	-2.4047
43	G	H	-1.6905
44	L	H	0.0000
45	E	H	-1.2375
46	W	H	0.0000
47	V	H	0.0000
48	S	H	0.0000
49	A	H	-0.3413
50	E	H	0.0000
51	D	H	-2.2317
52	G	H	0.0000
53	S	H	-2.2850
54	D	H	-3.3921
55	D	H	-3.6442
56	E	H	-3.2695
57	T	H	-1.6022
58	Y	H	-0.5704
59	Y	H	-0.5910
60	A	H	0.0000
61	D	H	-2.6247
62	S	H	-1.7621
63	V	H	0.0000
64	K	H	-2.4885
65	G	H	-1.6275
66	R	H	0.0000
67	F	H	0.0000
68	T	H	-0.8982
69	I	H	0.0000
70	S	H	-0.9421
71	R	H	-1.2844
72	D	H	-1.6698
73	N	H	-2.1491
74	S	H	-1.7596
75	K	H	-2.5010
76	N	H	-2.2908
77	T	H	-1.0918
78	L	H	0.0000
79	Y	H	-0.6610
80	L	H	0.0000
81	Q	H	-1.5743
82	M	H	0.0000
83	N	H	-1.4056
84	S	H	-1.2047
85	L	H	0.0000
86	R	H	-2.3125
87	A	H	-1.7200
88	E	H	-2.2222
89	D	H	0.0000
90	T	H	-0.4554
91	A	H	0.0000
92	V	H	0.5742
93	Y	H	0.0000
94	Y	H	0.0000
95	C	H	0.0000
96	A	H	0.0000
97	R	H	-0.2848
98	D	H	-0.5495
99	H	H	-0.4446
100	Y	H	-0.2535
101	D	H	-0.8044
102	S	H	-0.4462
103	G	H	-0.0601
104	Y	H	1.0485
105	Y	H	0.9237
106	R	H	0.0051
107	M	H	0.0000
108	D	H	-0.5555
109	E	H	0.0000
110	V	H	-0.2342
111	W	H	-0.4348
112	G	H	0.0000
113	Q	H	-1.3855
114	G	H	0.0000
115	T	H	0.0000
116	L	H	1.5866
117	V	H	0.0000
118	T	H	0.2747
119	V	H	0.0000
120	S	H	-0.6273
121	S	H	-0.6101
122	D	L	-2.3933
123	I	L	-1.7260
124	Q	L	-2.2407
125	M	L	0.0000
126	T	L	-1.3070
127	Q	L	0.0000
128	S	L	-0.9320
129	P	L	-0.6715
130	S	L	-0.9839
131	S	L	-1.2110
132	L	L	-0.7682
133	S	L	-1.1063
134	A	L	0.0000
135	S	L	-0.5443
136	V	L	0.0682
137	G	L	-0.8670
138	D	L	-1.7545
139	R	L	-2.3270
140	V	L	0.0000
141	T	L	-0.6152
142	I	L	0.0000
143	T	L	-0.7765
144	C	L	0.0000
145	R	L	-2.7349
146	A	L	0.0000
147	S	L	-2.1539
148	R	L	-2.7126
149	S	L	-1.4892
150	I	L	0.0000
151	S	L	-0.4777
152	S	L	-0.4198
153	Y	L	0.2489
154	L	L	0.0000
155	N	L	0.0000
156	W	L	0.0000
157	Y	L	0.0000
158	Q	L	0.0000
159	Q	L	0.0000
160	K	L	-1.5887
161	P	L	-1.1032
162	G	L	-1.6577
163	K	L	-2.5456
164	A	L	-1.6109
165	P	L	0.0000
166	K	L	-1.4464
167	L	L	0.0000
168	L	L	0.0000
169	I	L	0.0000
170	Y	L	-0.4404
171	A	L	-0.6651
172	E	L	-1.2853
173	D	L	-1.8991
174	S	L	-0.7877
175	L	L	-0.2065
176	Q	L	-0.4951
177	S	L	-0.4191
178	G	L	-0.4829
179	V	L	-0.3078
180	P	L	-0.3118
181	S	L	-0.4133
182	R	L	-0.7805
183	F	L	0.0000
184	S	L	-0.6939
185	G	L	0.0000
186	S	L	-1.1778
187	G	L	-1.2605
188	S	L	-0.9423
189	G	L	-1.1366
190	T	L	-1.6999
191	D	L	-1.9792
192	F	L	0.0000
193	T	L	-0.9405
194	L	L	0.0000
195	T	L	-0.5841
196	I	L	0.0000
197	S	L	-1.3154
198	S	L	-1.1748
199	L	L	0.0000
200	Q	L	-0.5829
201	P	L	-0.6246
202	E	L	-1.5305
203	D	L	0.0000
204	F	L	0.0277
205	A	L	0.0000
206	T	L	-1.1020
207	Y	L	0.0000
208	Y	L	0.0000
209	C	L	0.0000
210	E	L	0.0000
211	N	L	0.0000
212	W	L	0.5588
213	Y	L	0.8206
214	D	L	-0.7842
215	W	L	0.4112
216	P	L	-0.2264
217	R	L	-0.2981
218	T	L	-0.6986
219	F	L	0.0000
220	G	L	0.0000
221	Q	L	-1.9197
222	G	L	0.0000
223	T	L	0.0000
224	K	L	-2.2158
225	L	L	0.0000
226	E	L	-1.5587
227	I	L	0.0718
228	K	L	-1.3182