| Chain sequence(s) |
A: GGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSG
C: GGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSG B: GGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSG E: GGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSG D: GGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSG input PDB |
| Selected Chain(s) | A,C,B,E,D |
| Distance of aggregation | 10 Å |
| FoldX usage | Yes |
| Dynamic mode | No |
| Automated mutations | No |
| Mutated residues | AG321D,AG321C,AG321B,AG321A,AG321E |
| Energy difference between WT (input) and mutated protein (by FoldX) | 1.08763 kcal/mol
CAUTION: Your mutation/s can destabilize the protein structure |
| Downloads | Download all the data |
| Simulation log |
[INFO] Logger: Verbosity set to: 2 - [INFO] (00:00:01)
[WARNING] runJob: Working directory already exists (possibly overwriting previous results -ow
to prevent this behavior) (00:00:01)
[INFO] runJob: Starting aggrescan3d job on: input.pdb with all chain(s) selected (00:00:01)
[INFO] runJob: Creating pdb object from: input.pdb (00:00:01)
[INFO] FoldX: Starting FoldX energy minimalization (00:00:01)
[INFO] FoldX: Building mutant model (00:01:16)
[INFO] FoldX: Starting FoldX energy minimalization (00:01:28)
[INFO] Analysis: Starting Aggrescan3D on folded.pdb (00:02:08)
[INFO] Main: Simulation completed successfully. (00:02:10)
|
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 281 | G | A | -1.2771 | |
| 282 | G | A | -1.0412 | |
| 283 | F | A | 0.0000 | |
| 284 | G | A | -1.0891 | |
| 285 | N | A | 0.0000 | |
| 286 | Q | A | -1.7715 | |
| 287 | G | A | 0.0000 | |
| 288 | G | A | -0.9095 | |
| 289 | F | A | 0.0000 | |
| 290 | G | A | -1.4427 | |
| 291 | N | A | 0.0000 | |
| 292 | S | A | 0.0000 | |
| 293 | R | A | -3.9175 | |
| 294 | G | A | -2.7645 | |
| 295 | G | A | -1.8334 | |
| 296 | G | A | 0.0000 | |
| 297 | A | A | 0.1500 | |
| 298 | G | A | 0.6959 | |
| 299 | L | A | 1.4676 | |
| 300 | G | A | 0.0000 | |
| 301 | N | A | 0.0000 | |
| 302 | N | A | 0.0000 | |
| 303 | Q | A | 0.0000 | |
| 304 | G | A | 0.0000 | |
| 305 | S | A | 0.0000 | |
| 306 | N | A | 0.0000 | |
| 307 | M | A | 0.0000 | |
| 308 | G | A | 0.0000 | |
| 309 | G | A | 0.0000 | |
| 310 | G | A | 0.0000 | |
| 311 | M | A | 0.0000 | |
| 312 | N | A | 0.0000 | |
| 313 | F | A | 1.5987 | |
| 314 | G | A | 1.0047 | |
| 315 | A | A | 0.4918 | |
| 316 | F | A | 0.0000 | |
| 317 | S | A | -0.3074 | |
| 318 | I | A | 0.0000 | |
| 319 | N | A | -1.3138 | |
| 320 | P | A | 0.0000 | |
| 321 | G | A | -0.4767 | mutated: AG321A |
| 322 | M | A | 0.0000 | |
| 323 | M | A | 0.6214 | |
| 324 | A | A | 0.0000 | |
| 325 | A | A | 0.1670 | |
| 326 | A | A | -0.1708 | |
| 327 | Q | A | -0.4873 | |
| 328 | A | A | 0.0000 | |
| 329 | A | A | 0.0000 | |
| 330 | L | A | 0.0000 | |
| 331 | Q | A | 0.0000 | |
| 332 | S | A | 0.0000 | |
| 333 | S | A | 0.0000 | |
| 334 | W | A | 0.0000 | |
| 335 | G | A | 0.0000 | |
| 336 | M | A | 0.0000 | |
| 337 | M | A | 0.0000 | |
| 338 | G | A | 0.0000 | |
| 339 | M | A | 0.0000 | |
| 340 | L | A | 0.0000 | |
| 341 | A | A | 0.0000 | |
| 342 | S | A | 0.0000 | |
| 343 | Q | A | 0.0000 | |
| 344 | Q | A | -1.6947 | |
| 345 | N | A | 0.0000 | |
| 346 | Q | A | -2.0931 | |
| 347 | S | A | -1.5728 | |
| 348 | G | A | -1.2060 | |
| 349 | P | A | 0.0000 | |
| 350 | S | A | -1.0048 | |
| 351 | G | A | -1.0782 | |
| 281 | G | B | -1.2960 | |
| 282 | G | B | -1.0429 | |
| 283 | F | B | -0.3024 | |
| 284 | G | B | 0.0000 | |
| 285 | N | B | 0.0000 | |
| 286 | Q | B | -1.6947 | |
| 287 | G | B | 0.0000 | |
| 288 | G | B | -0.9630 | |
| 289 | F | B | 0.0000 | |
| 290 | G | B | 0.0000 | |
| 291 | N | B | 0.0000 | |
| 292 | S | B | 0.0000 | |
| 293 | R | B | -3.8500 | |
| 294 | G | B | -2.6161 | |
| 295 | G | B | -1.6540 | |
| 296 | G | B | 0.0000 | |
| 297 | A | B | 0.1947 | |
| 298 | G | B | 0.6468 | |
| 299 | L | B | 1.3022 | |
| 300 | G | B | 0.0000 | |
| 301 | N | B | 0.0000 | |
| 302 | N | B | 0.0000 | |
| 303 | Q | B | 0.0000 | |
| 304 | G | B | 0.0000 | |
| 305 | S | B | 0.0000 | |
| 306 | N | B | 0.0000 | |
| 307 | M | B | 0.0000 | |
| 308 | G | B | 0.0000 | |
| 309 | G | B | 0.0000 | |
| 310 | G | B | 0.0000 | |
| 311 | M | B | 0.0000 | |
| 312 | N | B | 0.0000 | |
| 313 | F | B | 2.0835 | |
| 314 | G | B | 1.0612 | |
| 315 | A | B | 0.4321 | |
| 316 | F | B | 0.0000 | |
| 317 | S | B | -0.4060 | |
| 318 | I | B | 0.0000 | |
| 319 | N | B | -1.4175 | |
| 320 | P | B | 0.0000 | |
| 321 | G | B | -0.5342 | mutated: AG321B |
| 322 | M | B | 0.0000 | |
| 323 | M | B | 0.5911 | |
| 324 | A | B | 0.0000 | |
| 325 | A | B | 0.0657 | |
| 326 | A | B | -0.2240 | |
| 327 | Q | B | -0.6990 | |
| 328 | A | B | 0.0000 | |
| 329 | A | B | 0.0000 | |
| 330 | L | B | 0.0000 | |
| 331 | Q | B | 0.0000 | |
| 332 | S | B | 0.0000 | |
| 333 | S | B | 0.0000 | |
| 334 | W | B | 0.0000 | |
| 335 | G | B | 0.0000 | |
| 336 | M | B | 0.0000 | |
| 337 | M | B | 0.0000 | |
| 338 | G | B | 0.0000 | |
| 339 | M | B | 0.0000 | |
| 340 | L | B | 0.0000 | |
| 341 | A | B | 0.0000 | |
| 342 | S | B | 0.0000 | |
| 343 | Q | B | 0.0000 | |
| 344 | Q | B | -1.0618 | |
| 345 | N | B | 0.0000 | |
| 346 | Q | B | -1.7730 | |
| 347 | S | B | 0.0000 | |
| 348 | G | B | -1.0370 | |
| 349 | P | B | 0.0000 | |
| 350 | S | B | -0.9476 | |
| 351 | G | B | -1.1132 | |
| 281 | G | C | -1.1040 | |
| 282 | G | C | -0.8847 | |
| 283 | F | C | -0.4501 | |
| 284 | G | C | -0.9452 | |
| 285 | N | C | -1.3495 | |
| 286 | Q | C | -1.9075 | |
| 287 | G | C | -1.1541 | |
| 288 | G | C | -0.9152 | |
| 289 | F | C | -0.3135 | |
| 290 | G | C | -1.1111 | |
| 291 | N | C | -1.4466 | |
| 292 | S | C | -2.3125 | |
| 293 | R | C | -3.1987 | |
| 294 | G | C | -2.5370 | |
| 295 | G | C | -1.6089 | |
| 296 | G | C | -1.4103 | |
| 297 | A | C | -0.0250 | |
| 298 | G | C | 0.6036 | |
| 299 | L | C | 1.6719 | |
| 300 | G | C | 0.0000 | |
| 301 | N | C | -0.1606 | |
| 302 | N | C | -0.8261 | |
| 303 | Q | C | -0.7112 | |
| 304 | G | C | 0.0000 | |
| 305 | S | C | -0.0369 | |
| 306 | N | C | -0.1742 | |
| 307 | M | C | 0.2119 | |
| 308 | G | C | -0.0896 | |
| 309 | G | C | -0.4567 | |
| 310 | G | C | -0.5062 | |
| 311 | M | C | 0.3093 | |
| 312 | N | C | 0.3783 | |
| 313 | F | C | 1.9368 | |
| 314 | G | C | 0.8966 | |
| 315 | A | C | 0.5389 | |
| 316 | F | C | 0.5176 | |
| 317 | S | C | -0.1271 | |
| 318 | I | C | -0.4569 | |
| 319 | N | C | -1.4238 | |
| 320 | P | C | -0.8826 | |
| 321 | G | C | -0.3988 | mutated: AG321C |
| 322 | M | C | 0.5738 | |
| 323 | M | C | 0.8822 | |
| 324 | A | C | 0.5077 | |
| 325 | A | C | 0.2033 | |
| 326 | A | C | -0.1217 | |
| 327 | Q | C | -0.4424 | |
| 328 | A | C | 0.0806 | |
| 329 | A | C | 0.1857 | |
| 330 | L | C | 0.0111 | |
| 331 | Q | C | -0.7546 | |
| 332 | S | C | -0.0906 | |
| 333 | S | C | 0.0941 | |
| 334 | W | C | 0.3590 | |
| 335 | G | C | 0.0000 | |
| 336 | M | C | 0.3302 | |
| 337 | M | C | 0.3866 | |
| 338 | G | C | 0.2972 | |
| 339 | M | C | 0.4209 | |
| 340 | L | C | 0.1115 | |
| 341 | A | C | -0.0223 | |
| 342 | S | C | -0.5388 | |
| 343 | Q | C | -1.3956 | |
| 344 | Q | C | -2.3354 | |
| 345 | N | C | -2.4633 | |
| 346 | Q | C | -2.4551 | |
| 347 | S | C | -1.4867 | |
| 348 | G | C | -1.2985 | |
| 349 | P | C | -0.7950 | |
| 350 | S | C | -0.8982 | |
| 351 | G | C | -0.8793 | |
| 281 | G | D | -1.1893 | |
| 282 | G | D | -0.7951 | |
| 283 | F | D | 0.0000 | |
| 284 | G | D | -1.0431 | |
| 285 | N | D | 0.0000 | |
| 286 | Q | D | -1.9328 | |
| 287 | G | D | 0.0000 | |
| 288 | G | D | -0.9791 | |
| 289 | F | D | 0.0000 | |
| 290 | G | D | -1.3969 | |
| 291 | N | D | 0.0000 | |
| 292 | S | D | 0.0000 | |
| 293 | R | D | -3.6592 | |
| 294 | G | D | -2.4226 | |
| 295 | G | D | -1.6845 | |
| 296 | G | D | 0.0000 | |
| 297 | A | D | 0.1703 | |
| 298 | G | D | 0.5094 | |
| 299 | L | D | 1.2767 | |
| 300 | G | D | 0.0000 | |
| 301 | N | D | 0.0000 | |
| 302 | N | D | 0.0000 | |
| 303 | Q | D | 0.0000 | |
| 304 | G | D | 0.0000 | |
| 305 | S | D | 0.0000 | |
| 306 | N | D | 0.0000 | |
| 307 | M | D | 0.0000 | |
| 308 | G | D | 0.0000 | |
| 309 | G | D | 0.0000 | |
| 310 | G | D | 0.0000 | |
| 311 | M | D | 0.0000 | |
| 312 | N | D | 0.0000 | |
| 313 | F | D | 2.0819 | |
| 314 | G | D | 1.2948 | |
| 315 | A | D | 0.6262 | |
| 316 | F | D | 0.0000 | |
| 317 | S | D | -0.3698 | |
| 318 | I | D | 0.0000 | |
| 319 | N | D | -1.4124 | |
| 320 | P | D | 0.0000 | |
| 321 | G | D | -0.5756 | mutated: AG321D |
| 322 | M | D | 0.0000 | |
| 323 | M | D | 0.6812 | |
| 324 | A | D | 0.0000 | |
| 325 | A | D | 0.0602 | |
| 326 | A | D | -0.5030 | |
| 327 | Q | D | -0.9205 | |
| 328 | A | D | 0.0000 | |
| 329 | A | D | 0.0000 | |
| 330 | L | D | 0.0000 | |
| 331 | Q | D | 0.0000 | |
| 332 | S | D | 0.0000 | |
| 333 | S | D | 0.0000 | |
| 334 | W | D | 0.0000 | |
| 335 | G | D | 0.0000 | |
| 336 | M | D | 0.0000 | |
| 337 | M | D | 0.0000 | |
| 338 | G | D | 0.0000 | |
| 339 | M | D | 0.0000 | |
| 340 | L | D | 0.0000 | |
| 341 | A | D | 0.0000 | |
| 342 | S | D | 0.0000 | |
| 343 | Q | D | 0.0000 | |
| 344 | Q | D | -1.2306 | |
| 345 | N | D | 0.0000 | |
| 346 | Q | D | -1.9670 | |
| 347 | S | D | 0.0000 | |
| 348 | G | D | -1.1392 | |
| 349 | P | D | 0.0000 | |
| 350 | S | D | -0.9166 | |
| 351 | G | D | -1.1016 | |
| 281 | G | E | -1.0543 | |
| 282 | G | E | -0.5254 | |
| 283 | F | E | 0.1932 | |
| 284 | G | E | -0.8522 | |
| 285 | N | E | -1.0050 | |
| 286 | Q | E | -1.9032 | |
| 287 | G | E | -1.0526 | |
| 288 | G | E | -0.9040 | |
| 289 | F | E | -0.4911 | |
| 290 | G | E | -1.1158 | |
| 291 | N | E | -1.3738 | |
| 292 | S | E | -1.9625 | |
| 293 | R | E | -3.1787 | |
| 294 | G | E | -2.1657 | |
| 295 | G | E | -1.6934 | |
| 296 | G | E | -1.0199 | |
| 297 | A | E | -0.0669 | |
| 298 | G | E | 0.2599 | |
| 299 | L | E | 1.4208 | |
| 300 | G | E | 0.0984 | |
| 301 | N | E | -0.4955 | |
| 302 | N | E | -1.4267 | |
| 303 | Q | E | -1.1756 | |
| 304 | G | E | -0.8398 | |
| 305 | S | E | -0.5765 | |
| 306 | N | E | -0.7961 | |
| 307 | M | E | 0.0000 | |
| 308 | G | E | -0.2600 | |
| 309 | G | E | -0.3617 | |
| 310 | G | E | 0.0000 | |
| 311 | M | E | 0.8810 | |
| 312 | N | E | 1.2208 | |
| 313 | F | E | 2.3397 | |
| 314 | G | E | 0.9924 | |
| 315 | A | E | 0.5868 | |
| 316 | F | E | 0.3941 | |
| 317 | S | E | -0.2320 | |
| 318 | I | E | -0.3395 | |
| 319 | N | E | -1.5007 | |
| 320 | P | E | -0.7630 | |
| 321 | G | E | -0.4565 | mutated: AG321E |
| 322 | M | E | 0.5413 | |
| 323 | M | E | 1.1269 | |
| 324 | A | E | 0.0000 | |
| 325 | A | E | -0.0225 | |
| 326 | A | E | -0.6166 | |
| 327 | Q | E | -1.2258 | |
| 328 | A | E | -0.4779 | |
| 329 | A | E | 0.0000 | |
| 330 | L | E | -0.4033 | |
| 331 | Q | E | -1.2980 | |
| 332 | S | E | -0.5213 | |
| 333 | S | E | -0.2309 | |
| 334 | W | E | 0.1730 | |
| 335 | G | E | 0.0676 | |
| 336 | M | E | 0.2605 | |
| 337 | M | E | 0.0291 | |
| 338 | G | E | -0.1996 | |
| 339 | M | E | 0.1498 | |
| 340 | L | E | -0.1987 | |
| 341 | A | E | -0.0007 | |
| 342 | S | E | -0.1507 | |
| 343 | Q | E | -0.7789 | |
| 344 | Q | E | -1.5697 | |
| 345 | N | E | -2.0173 | |
| 346 | Q | E | -2.1862 | |
| 347 | S | E | -1.5615 | |
| 348 | G | E | -1.1072 | |
| 349 | P | E | -0.7616 | |
| 350 | S | E | -0.8506 | |
| 351 | G | E | -1.0643 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine