Project name: VHL-12VC1.2

Chain sequence(s)	A: MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQRMGDSSSSGVSSVPTELEVVAATPTSLLISWDAPAVTVFFYVITYGETGHGVGAFQAFAVPGSRSTATISGLEPGVDYTITVYARGYSKQGPYKPSPISINYRT
Selected Chain(s)	A
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with A chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:06:17)
[INFO]       Main:     Simulation completed successfully.                                          (00:06:19)

• Minimal score value: -4.2265
• Maximal score value: 1.7608
• Average score: -0.8742
• Total score value: -273.6315

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	M	A	0.1036
2	P	A	-1.1682
3	R	A	-2.7740
4	R	A	-3.1279
5	A	A	-2.3327
6	E	A	-2.6469
7	N	A	-2.9088
8	W	A	0.0000
9	D	A	-3.5973
10	E	A	-3.2840
11	A	A	-2.3671
12	E	A	-2.5214
13	V	A	-1.3923
14	G	A	-1.4720
15	A	A	-1.6435
16	E	A	-2.5938
17	E	A	-2.8796
18	A	A	0.0000
19	G	A	-1.0299
20	V	A	0.1466
21	E	A	-1.3057
22	E	A	-1.0862
23	Y	A	-0.8326
24	G	A	-0.9144
25	P	A	-1.3887
26	E	A	0.0000
27	E	A	-3.1819
28	D	A	-2.9122
29	G	A	-2.1253
30	G	A	-2.6578
31	E	A	-3.6725
32	E	A	-3.5011
33	S	A	-2.1478
34	G	A	-1.6485
35	A	A	-1.2756
36	E	A	-2.0488
37	E	A	0.0000
38	S	A	-1.3420
39	G	A	-1.4272
40	P	A	-1.7698
41	E	A	-2.7314
42	E	A	-2.9758
43	S	A	-1.8958
44	G	A	0.0000
45	P	A	-0.9819
46	E	A	-2.4441
47	E	A	0.0000
48	L	A	0.2583
49	G	A	-0.4356
50	A	A	-1.5766
51	E	A	-2.8570
52	E	A	-4.1387
53	E	A	-4.2265
54	M	A	-3.2058
55	E	A	-3.0200
56	A	A	-1.6818
57	G	A	-2.3033
58	R	A	-2.7108
59	P	A	-2.2264
60	R	A	0.0000
61	P	A	0.0000
62	V	A	1.3993
63	L	A	0.0000
64	R	A	0.0000
65	S	A	0.0000
66	V	A	-0.1114
67	N	A	0.0000
68	S	A	-1.2460
69	R	A	-2.3361
70	E	A	-2.6909
71	P	A	-1.9852
72	S	A	0.0000
73	Q	A	-1.4494
74	V	A	0.0000
75	I	A	0.0000
76	F	A	0.0000
77	C	A	0.0000
78	N	A	0.0000
79	R	A	0.0000
80	S	A	0.0000
81	P	A	-0.9697
82	R	A	-0.4165
83	V	A	0.0000
84	V	A	0.0000
85	L	A	0.0000
86	P	A	0.0000
87	V	A	0.0000
88	W	A	0.0000
89	L	A	0.0000
90	N	A	0.0000
91	F	A	0.0000
92	D	A	-0.4673
93	G	A	0.0000
94	E	A	-0.4364
95	P	A	0.0000
96	Q	A	0.0000
97	P	A	0.0000
98	Y	A	0.0000
99	P	A	-0.4608
100	T	A	0.0000
101	L	A	0.0000
102	P	A	-0.7172
103	P	A	-1.3458
104	G	A	0.0000
105	T	A	0.0000
106	G	A	0.0000
107	R	A	0.0000
108	R	A	-2.2272
109	I	A	0.0000
110	H	A	-1.9708
111	S	A	0.0000
112	Y	A	-1.7283
113	R	A	-2.1100
114	G	A	-0.6315
115	H	A	0.0000
116	L	A	0.5933
117	W	A	0.0000
118	L	A	0.0000
119	F	A	0.0000
120	R	A	-1.2254
121	D	A	0.0000
122	A	A	0.0000
123	G	A	0.0000
124	T	A	-1.0104
125	H	A	-1.7813
126	D	A	-2.3961
127	G	A	-1.4786
128	L	A	0.0000
129	L	A	0.0000
130	V	A	0.0000
131	N	A	-1.5545
132	Q	A	-1.3886
133	T	A	-1.0593
134	E	A	-0.9801
135	L	A	0.0402
136	F	A	0.0000
137	V	A	1.1697
138	P	A	0.0000
139	S	A	-0.2220
140	L	A	-0.1631
141	N	A	-0.1860
142	V	A	0.7661
143	D	A	-1.2203
144	G	A	-1.1345
145	Q	A	-0.9992
146	P	A	-0.7789
147	I	A	0.2747
148	F	A	0.0000
149	A	A	0.0000
150	N	A	0.0000
151	I	A	0.0000
152	T	A	-0.3010
153	L	A	0.3294
154	P	A	0.3756
155	V	A	1.7608
156	Y	A	0.5386
157	T	A	0.1923
158	L	A	0.4260
159	K	A	-1.3177
160	E	A	-1.1743
161	R	A	-1.2759
162	C	A	-0.2612
163	L	A	0.0000
164	Q	A	-0.1984
165	V	A	0.9751
166	V	A	0.9395
167	R	A	0.0000
168	S	A	0.3125
169	L	A	1.5312
170	V	A	-0.1869
171	K	A	-2.0710
172	P	A	-2.6215
173	E	A	-3.4996
174	N	A	-3.2250
175	Y	A	0.0000
176	R	A	-3.9532
177	R	A	-3.3744
178	L	A	-1.7516
179	D	A	-1.8626
180	I	A	-0.0125
181	V	A	1.0111
182	R	A	-1.5292
183	S	A	-0.7499
184	L	A	-0.9754
185	Y	A	-2.2076
186	E	A	-3.3436
187	D	A	-2.4718
188	L	A	0.0000
189	E	A	-3.2797
190	D	A	-3.1457
191	H	A	-2.1892
192	P	A	-1.4505
193	N	A	-1.5028
194	V	A	-1.2855
195	Q	A	-2.6614
196	K	A	-3.1259
197	D	A	-1.9214
198	L	A	-2.2310
199	E	A	-3.3802
200	R	A	-2.4717
201	L	A	-1.6982
202	T	A	-1.6370
203	Q	A	-1.9138
204	E	A	-1.6693
205	R	A	-1.5345
206	I	A	-0.6211
207	A	A	-1.1714
208	H	A	-1.8643
209	Q	A	-2.5237
210	R	A	-2.2398
211	M	A	-0.9538
212	G	A	-1.8071
213	D	A	-2.4957
214	S	A	-1.8098
215	S	A	-1.2936
216	S	A	-0.5350
217	S	A	-0.0138
218	G	A	0.0614
219	V	A	1.5423
220	S	A	0.7706
221	S	A	0.6163
222	V	A	0.7065
223	P	A	0.0000
224	T	A	-1.4714
225	E	A	-2.6566
226	L	A	0.0000
227	E	A	-1.7287
228	V	A	0.2307
229	V	A	1.5022
230	A	A	0.8920
231	A	A	0.3177
232	T	A	-0.1604
233	P	A	-1.1559
234	T	A	-1.0044
235	S	A	-0.5069
236	L	A	0.0000
237	L	A	0.7838
238	I	A	0.0000
239	S	A	-0.8675
240	W	A	0.0000
241	D	A	-2.4542
242	A	A	-1.0628
243	P	A	0.1674
244	A	A	0.5170
245	V	A	0.9697
246	T	A	0.4270
247	V	A	0.3887
248	F	A	0.4541
249	F	A	0.0000
250	Y	A	0.0000
251	V	A	0.0000
252	I	A	0.0000
253	T	A	0.0000
254	Y	A	-0.0304
255	G	A	0.0000
256	E	A	-1.7981
257	T	A	-1.4335
258	G	A	-1.1936
259	H	A	-1.3278
260	G	A	-0.4948
261	V	A	0.7103
262	G	A	-0.4152
263	A	A	-0.2386
264	F	A	0.0000
265	Q	A	-0.5434
266	A	A	-0.1858
267	F	A	0.1757
268	A	A	0.1487
269	V	A	0.0000
270	P	A	-0.6943
271	G	A	-0.4523
272	S	A	-0.8361
273	R	A	-1.6620
274	S	A	-1.2788
275	T	A	-0.6054
276	A	A	0.0000
277	T	A	0.1237
278	I	A	0.0000
279	S	A	-0.4545
280	G	A	-0.8865
281	L	A	0.0000
282	E	A	-2.5347
283	P	A	-1.7958
284	G	A	-1.5725
285	V	A	-1.6581
286	D	A	-2.4429
287	Y	A	0.0000
288	T	A	-0.9292
289	I	A	0.0000
290	T	A	-0.0367
291	V	A	0.0000
292	Y	A	0.0000
293	A	A	0.0000
294	R	A	0.0958
295	G	A	0.4955
296	Y	A	0.4939
297	S	A	-0.1113
298	K	A	-0.6424
299	Q	A	-0.5796
300	G	A	-0.7531
301	P	A	-1.0205
302	Y	A	0.0000
303	K	A	-1.7556
304	P	A	-0.7014
305	S	A	-0.3655
306	P	A	-0.0301
307	I	A	0.1668
308	S	A	-0.5200
309	I	A	-0.6483
310	N	A	-1.7669
311	Y	A	-1.5293
312	R	A	-2.7150
313	T	A	-1.7654