Project name: Muhammad Sohaib Hassan

Chain sequence(s)	H: EVQLLESGGGLVQPGGSLRLSCAASDDTFSRYDMSWVRQAPGKGLEWVSAEDDSDDEDYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARDHYDSGYYRMDEVWGQGTLVTVSS; L: DIQMTQSPSSLSASVGDRVTITCRASRSISSDLNWYQQKPGKAPKLLIYAEDSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCEDWYDWPRDFGQGTKLEIK
Selected Chain(s)	H,L
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:02:42)
[INFO]       Main:     Simulation completed successfully.                                          (00:02:44)

• Minimal score value: -3.2506
• Maximal score value: 1.5368
• Average score: -0.7339
• Total score value: -168.0555

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
0	E	H	-2.1640
1	V	H	-1.1937
2	Q	H	-1.2873
3	L	H	0.0000
4	L	H	0.2414
5	E	H	0.0000
6	S	H	-0.4322
7	G	H	-0.6080
8	G	H	0.0300
9	G	H	0.7884
10	L	H	1.2502
11	V	H	0.0000
12	Q	H	-1.5512
13	P	H	-1.9334
14	G	H	-1.6639
15	G	H	-1.1361
16	S	H	-1.3993
17	L	H	-1.0576
18	R	H	-2.0948
19	L	H	0.0000
20	S	H	-0.5313
21	C	H	0.0000
22	A	H	-0.3702
23	A	H	0.0000
24	S	H	-1.6815
25	D	H	-2.6058
26	D	H	-2.1535
27	T	H	-1.4188
28	F	H	0.0000
29	S	H	-1.8558
30	R	H	-1.8722
31	Y	H	-0.5899
32	D	H	0.0000
33	M	H	0.0000
34	S	H	0.0000
35	W	H	0.0000
36	V	H	0.0000
37	R	H	0.0000
38	Q	H	-0.7371
39	A	H	-1.1314
40	P	H	-0.8855
41	G	H	-1.5250
42	K	H	-2.3933
43	G	H	-1.6861
44	L	H	0.0000
45	E	H	-1.2273
46	W	H	0.0000
47	V	H	0.0000
48	S	H	0.0000
49	A	H	0.0000
50	E	H	0.0000
51	D	H	-1.9252
52	D	H	0.0000
53	S	H	-1.9940
54	D	H	-3.0107
55	D	H	-3.0373
56	E	H	-3.2506
57	D	H	-2.1823
58	Y	H	-0.9139
59	Y	H	-0.9206
60	A	H	-1.3936
61	D	H	-2.4963
62	S	H	-1.7922
63	V	H	0.0000
64	K	H	-2.5982
65	G	H	-1.8127
66	R	H	-1.5958
67	F	H	0.0000
68	T	H	-0.9516
69	I	H	0.0000
70	S	H	-0.8897
71	R	H	-1.1814
72	D	H	-1.7328
73	N	H	-2.2078
74	S	H	-1.7796
75	K	H	-2.5457
76	N	H	-2.2763
77	T	H	-1.1961
78	L	H	0.0000
79	Y	H	-0.5360
80	L	H	0.0000
81	Q	H	-1.2471
82	M	H	0.0000
83	N	H	-1.5876
84	S	H	-1.4675
85	L	H	0.0000
86	R	H	-2.9819
87	A	H	-2.0848
88	E	H	-2.4527
89	D	H	0.0000
90	T	H	-0.5850
91	A	H	0.0000
92	V	H	0.5046
93	Y	H	0.0000
94	Y	H	0.0000
95	C	H	0.0000
96	A	H	0.0000
97	R	H	0.0000
98	D	H	0.0000
99	H	H	-0.2061
100	Y	H	0.0745
101	D	H	-1.2510
102	S	H	-0.3266
103	G	H	0.2838
104	Y	H	1.3702
105	Y	H	1.5368
106	R	H	0.1772
107	M	H	0.0000
108	D	H	0.0000
109	E	H	0.0000
110	V	H	0.2387
111	W	H	-0.1241
112	G	H	0.0000
113	Q	H	-1.4759
114	G	H	0.0000
115	T	H	0.3422
116	L	H	1.4091
117	V	H	0.0000
118	T	H	0.1350
119	V	H	0.0000
120	S	H	-0.7077
121	S	H	-0.5936
122	D	L	-2.4661
123	I	L	-2.0988
124	Q	L	-2.4428
125	M	L	0.0000
126	T	L	-1.4648
127	Q	L	0.0000
128	S	L	-0.8609
129	P	L	-0.4807
130	S	L	-0.8793
131	S	L	-0.9295
132	L	L	-0.4046
133	S	L	-0.7223
134	A	L	0.0000
135	S	L	-0.2983
136	V	L	0.6188
137	G	L	-0.6789
138	D	L	-1.6444
139	R	L	-2.2858
140	V	L	0.0000
141	T	L	-0.5936
142	I	L	0.0000
143	T	L	-0.7824
144	C	L	0.0000
145	R	L	-2.8404
146	A	L	0.0000
147	S	L	-2.3097
148	R	L	-2.8227
149	S	L	-1.4707
150	I	L	0.0000
151	S	L	-0.6085
152	S	L	-0.5799
153	D	L	-0.4190
154	L	L	0.0000
155	N	L	0.0000
156	W	L	0.0000
157	Y	L	0.0000
158	Q	L	0.0000
159	Q	L	0.0000
160	K	L	-1.6119
161	P	L	-1.1862
162	G	L	-1.6462
163	K	L	-2.5717
164	A	L	-1.6495
165	P	L	0.0000
166	K	L	-1.5482
167	L	L	0.0000
168	L	L	0.0000
169	I	L	0.0000
170	Y	L	0.0000
171	A	L	-0.8682
172	E	L	-1.4306
173	D	L	-2.0540
174	S	L	-0.8947
175	L	L	-0.2728
176	Q	L	-0.4958
177	S	L	-0.3671
178	G	L	-0.5100
179	V	L	-0.3611
180	P	L	-0.3224
181	S	L	-0.3990
182	R	L	-0.7518
183	F	L	0.0000
184	S	L	-0.7114
185	G	L	-1.0066
186	S	L	-1.1818
187	G	L	-1.2861
188	S	L	-1.2230
189	G	L	-1.2973
190	T	L	-1.8971
191	D	L	-2.0996
192	F	L	0.0000
193	T	L	-0.9340
194	L	L	0.0000
195	T	L	-0.5716
196	I	L	0.0000
197	S	L	-1.2952
198	S	L	-1.0610
199	L	L	0.0000
200	Q	L	-0.5752
201	P	L	-0.6542
202	E	L	-1.6715
203	D	L	0.0000
204	F	L	-0.0504
205	A	L	0.0000
206	T	L	0.0000
207	Y	L	0.0000
208	Y	L	0.0000
209	C	L	0.0000
210	E	L	0.0000
211	D	L	0.0000
212	W	L	0.2104
213	Y	L	0.4770
214	D	L	-1.2214
215	W	L	-0.4735
216	P	L	-0.7539
217	R	L	0.0000
218	D	L	-1.2484
219	F	L	0.0000
220	G	L	0.0000
221	Q	L	-1.9945
222	G	L	-1.3279
223	T	L	0.0000
224	K	L	-1.7098
225	L	L	0.0000
226	E	L	-1.0375
227	I	L	0.1855
228	K	L	-1.1229