Project name: obj1 [mutate: GI9C, LN11C, LP45C]

Chain sequence(s)	C: EVQLVESGGGLVQPGGSLRLSCAASDFTFRSYEMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARLRDGFNKGFDYWGQGTLVTVSS
Selected Chain(s)	C
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No
Mutated residues	LP45C,GI9C,LN11C
Energy difference between WT (input) and mutated protein (by FoldX)	17.778 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with C chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       FoldX:    Building mutant model                                                       (00:00:24)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:33)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:00:56)
[INFO]       Main:     Simulation completed successfully.                                          (00:00:57)

• Minimal score value: -3.3308
• Maximal score value: 1.5458
• Average score: -0.7716
• Total score value: -92.5882

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	C	-2.0068
2	V	C	-0.9146
3	Q	C	-1.2407
4	L	C	0.0000
5	V	C	0.5712
6	E	C	0.3119
7	S	C	-0.2560
8	G	C	-0.5007
9	I	C	0.0444	mutated: GI9C
10	G	C	-0.1370
11	N	C	-1.2509	mutated: LN11C
12	V	C	-1.2966
13	Q	C	-1.9598
14	P	C	-1.7865
15	G	C	-1.4104
16	G	C	-1.2724
17	S	C	-1.2274
18	L	C	-1.1429
19	R	C	-2.0209
20	L	C	0.0000
21	S	C	-0.4552
22	C	C	0.0000
23	A	C	-0.2019
24	A	C	0.0000
25	S	C	-0.2017
26	D	C	0.0000
27	F	C	1.5458
28	T	C	0.2525
29	F	C	0.0000
30	R	C	-2.0325
31	S	C	-0.8863
32	Y	C	-1.2160
33	E	C	-1.1431
34	M	C	0.0000
35	S	C	0.0000
36	W	C	0.0000
37	V	C	0.0000
38	R	C	0.0000
39	Q	C	-1.0080
40	A	C	-1.3451
41	P	C	-1.3153
42	G	C	-1.6084
43	K	C	-2.5152
44	G	C	-1.8718
45	P	C	-1.2850	mutated: LP45C
46	E	C	-1.2025
47	W	C	-0.0399
48	V	C	0.0000
49	S	C	0.0000
50	A	C	0.0000
51	I	C	0.0000
52	S	C	-0.5831
53	G	C	-1.2447
54	S	C	-1.2286
55	G	C	-1.0817
56	G	C	-0.7345
57	S	C	-0.3025
58	T	C	0.1989
59	Y	C	0.6077
60	Y	C	-0.3559
61	A	C	-1.1386
62	D	C	-2.3443
63	S	C	-1.7150
64	V	C	0.0000
65	K	C	-2.3847
66	G	C	-1.6175
67	R	C	0.0000
68	F	C	0.0000
69	T	C	-0.6722
70	I	C	0.0000
71	S	C	-0.5624
72	R	C	-1.3626
73	D	C	-1.9805
74	N	C	-2.1901
75	S	C	-1.7905
76	K	C	-2.3163
77	N	C	-1.6491
78	T	C	0.0000
79	L	C	0.0000
80	Y	C	-0.6688
81	L	C	0.0000
82	Q	C	-1.2340
83	M	C	0.0000
84	N	C	-1.3329
85	S	C	-1.2323
86	L	C	0.0000
87	R	C	-2.4803
88	A	C	-1.9064
89	E	C	-2.3525
90	D	C	0.0000
91	T	C	-0.8049
92	A	C	0.0000
93	I	C	0.8585
94	Y	C	0.0000
95	Y	C	0.4366
96	C	C	0.0000
97	A	C	0.0000
98	R	C	0.0000
99	L	C	0.0000
100	R	C	-3.1852
101	D	C	-3.3308
102	G	C	-2.0842
103	F	C	-1.2284
104	N	C	-2.4259
105	K	C	-3.1810
106	G	C	-1.8986
107	F	C	-0.9639
108	D	C	-1.0914
109	Y	C	-0.1971
110	W	C	0.5836
111	G	C	-0.0527
112	Q	C	-0.8762
113	G	C	0.1518
114	T	C	0.0000
115	L	C	1.4663
116	V	C	0.0000
117	T	C	-0.6410
118	V	C	0.0000
119	S	C	-1.3536
120	S	C	-1.0890