Project name: 8A9D6

Chain sequence(s)	H: EVQLVETGGGLVQPKGSLKLSCAASGFTFNITAMNWVRQAPGKGLEWVARIRSKSNNYATNYADSVKDRFTISRDDSQSILYLQMNNLKTEDTAMYYCVRGGSPYAMDYWGQGTSVTVSS; L: DIVMTQSPATLSVTPGDRVSLSCRASQSISDFLHWYQQKSHESPRLLIKYASQSISGIPSRFSGSGSGSDFTLSINNVEPEDVGVYYCQNGHSFPPTFGAGTKLELK
Selected Chain(s)	H,L
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:01:02)
[INFO]       Main:     Simulation completed successfully.                                          (00:01:03)

• Minimal score value: -3.1124
• Maximal score value: 1.3976
• Average score: -0.6075
• Total score value: -137.9038

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	H	-2.0225
2	V	H	-1.0495
3	Q	H	-0.8640
4	L	H	0.0000
5	V	H	1.2715
6	E	H	0.0000
7	T	H	-0.1336
8	G	H	-0.6289
9	G	H	-0.2566
11	G	H	0.4155
12	L	H	1.1633
13	V	H	-0.1804
14	Q	H	-1.7060
15	P	H	-2.2942
16	K	H	-3.1124
17	G	H	-1.8989
18	S	H	-1.6134
19	L	H	-0.7436
20	K	H	-1.2677
21	L	H	0.0000
22	S	H	-0.0119
23	C	H	0.0000
24	A	H	0.3435
25	A	H	0.0000
26	S	H	-0.6913
27	G	H	-1.1296
28	F	H	-0.2337
29	T	H	0.2646
30	F	H	0.0000
35	N	H	-0.6554
36	I	H	0.8963
37	T	H	0.1997
38	A	H	-0.5146
39	M	H	0.0000
40	N	H	0.0000
41	W	H	0.0000
42	V	H	0.0000
43	R	H	0.0000
44	Q	H	-0.8735
45	A	H	-1.2475
46	P	H	-1.0177
47	G	H	-1.4538
48	K	H	-2.1748
49	G	H	-1.1699
50	L	H	0.0000
51	E	H	-0.8951
52	W	H	0.0000
53	V	H	0.0000
54	A	H	0.0000
55	R	H	-0.1806
56	I	H	0.0000
57	R	H	-1.5456
58	S	H	0.0000
59	K	H	-2.4727
60	S	H	-1.7669
61	N	H	-2.3935
62	N	H	-2.5026
63	Y	H	-1.5693
64	A	H	-1.1171
65	T	H	-0.4422
66	N	H	0.0000
67	Y	H	-0.7290
68	A	H	-1.1650
69	D	H	-2.4800
70	S	H	-1.8049
71	V	H	0.0000
72	K	H	-2.6548
74	D	H	-2.9181
75	R	H	-2.4323
76	F	H	0.0000
77	T	H	-0.9480
78	I	H	0.0000
79	S	H	-0.3224
80	R	H	-0.8403
81	D	H	-1.0400
82	D	H	-1.1961
83	S	H	-1.1738
84	Q	H	-1.5467
85	S	H	-0.9498
86	I	H	-0.2326
87	L	H	0.0000
88	Y	H	-0.2385
89	L	H	0.0000
90	Q	H	-0.9935
91	M	H	0.0000
92	N	H	-2.2043
93	N	H	-3.0168
94	L	H	0.0000
95	K	H	-2.6596
96	T	H	-1.8571
97	E	H	-2.2255
98	D	H	0.0000
99	T	H	-0.6931
100	A	H	0.0000
101	M	H	-0.0633
102	Y	H	0.0000
103	Y	H	0.0000
104	C	H	0.0000
105	V	H	0.0000
106	R	H	-0.2464
107	G	H	0.0000
108	G	H	0.2184
109	S	H	0.0067
110	P	H	0.6099
113	Y	H	1.0372
114	A	H	0.0000
115	M	H	0.0000
116	D	H	-0.2377
117	Y	H	0.2176
118	W	H	-0.0079
119	G	H	0.0000
120	Q	H	-1.0624
121	G	H	0.0000
122	T	H	0.0000
123	S	H	-0.2024
124	V	H	0.0000
125	T	H	-0.0786
126	V	H	0.0000
127	S	H	-0.8313
128	S	H	-0.7091
1	D	L	-1.1644
2	I	L	0.0000
3	V	L	0.8312
4	M	L	0.0000
5	T	L	-0.4830
6	Q	L	0.0000
7	S	L	-0.6353
8	P	L	-0.3280
9	A	L	-0.2216
10	T	L	-0.4131
11	L	L	-0.3422
12	S	L	-0.6603
13	V	L	0.0000
14	T	L	-1.5315
15	P	L	-1.6431
16	G	L	-2.0536
17	D	L	-2.5879
18	R	L	-3.0952
19	V	L	0.0000
20	S	L	-0.8391
21	L	L	0.0000
22	S	L	-0.9363
23	C	L	0.0000
24	R	L	-2.4141
25	A	L	0.0000
26	S	L	-0.9185
27	Q	L	-1.4244
28	S	L	-1.3995
29	I	L	0.0000
36	S	L	-1.0337
37	D	L	-1.3949
38	F	L	0.0397
39	L	L	0.0000
40	H	L	0.0000
41	W	L	0.0000
42	Y	L	0.0000
43	Q	L	0.0000
44	Q	L	-0.6800
45	K	L	-0.9599
46	S	L	-0.9034
47	H	L	-1.6736
48	E	L	-1.6498
49	S	L	-1.0019
50	P	L	0.0000
51	R	L	-0.5788
52	L	L	0.0000
53	L	L	0.0000
54	I	L	0.0000
55	K	L	-0.3644
56	Y	L	-0.3063
57	A	L	0.0000
65	S	L	-0.9148
66	Q	L	-1.1103
67	S	L	-0.6563
68	I	L	-0.2962
69	S	L	-0.2567
70	G	L	-0.4262
71	I	L	-0.1493
72	P	L	-0.2590
74	S	L	-0.6007
75	R	L	-1.2509
76	F	L	0.0000
77	S	L	-0.7245
78	G	L	-0.5958
79	S	L	-0.9022
80	G	L	-1.2560
83	S	L	-1.3479
84	G	L	-1.3610
85	S	L	-1.9811
86	D	L	-2.5260
87	F	L	0.0000
88	T	L	-0.8482
89	L	L	0.0000
90	S	L	0.0000
91	I	L	0.0000
92	N	L	-2.6191
93	N	L	-2.6210
94	V	L	0.0000
95	E	L	-1.5742
96	P	L	-1.0301
97	E	L	-0.9093
98	D	L	0.0000
99	V	L	0.0889
100	G	L	-0.3130
101	V	L	-0.0033
102	Y	L	0.0000
103	Y	L	0.0000
104	C	L	0.0000
105	Q	L	0.0000
106	N	L	0.0000
107	G	L	0.0000
108	H	L	-0.2832
109	S	L	0.2812
114	F	L	1.3976
115	P	L	0.3193
116	P	L	0.1649
117	T	L	0.1773
118	F	L	0.3179
119	G	L	0.0000
120	A	L	-0.1744
121	G	L	0.0000
122	T	L	0.0000
123	K	L	-0.8008
124	L	L	0.0000
125	E	L	-0.6608
126	L	L	-0.7795
127	K	L	-1.7041