Project name: 3e99768714ce0a

Chain sequence(s)	H: EVQLVESGGGLVQPGGSLRISCAASGLTFTGYWMNWVRQAPGKGLEWVANIKEDGSEKYYVDSVKGRFTISRDNAKNSLYLQMNSLRVEDTAVYYCARLRWLRGNFDHWGQGTLVTVSSASTKGPSVFPLAPTAALGCLVKDYFPEPVTVSWNGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKV; L: FMLTQPHSVSESPGKTVTISCTGSNGYIANNYVQWYQQRPGSVPTVVIYEDNQRPSGVPDRFSGSIDSSSNSASLIISGLKTEDEADYYCQSYDTPNVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPT
Selected Chain(s)	H,L
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:03:01)
[INFO]       Main:     Simulation completed successfully.                                          (00:03:03)

• Minimal score value: -3.095
• Maximal score value: 1.1174
• Average score: -0.6629
• Total score value: -275.1119

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	H	-1.9998
2	V	H	-1.0857
3	Q	H	-1.2308
4	L	H	0.0000
5	V	H	0.4340
6	E	H	-0.2671
7	S	H	-0.6592
8	G	H	-0.9107
9	G	H	-0.9025
11	G	H	-0.6518
12	L	H	-0.1972
13	V	H	0.0000
14	Q	H	-1.4884
15	P	H	-1.3410
16	G	H	-1.3481
17	G	H	-1.2084
18	S	H	-1.3066
19	L	H	-1.1223
20	R	H	-2.0256
21	I	H	0.0000
22	S	H	-0.4545
23	C	H	0.0000
24	A	H	-0.4475
25	A	H	-0.5628
26	S	H	-0.8806
27	G	H	-1.0009
28	L	H	-0.4909
29	T	H	-0.2993
30	F	H	0.0000
35	T	H	-1.2042
36	G	H	-1.0043
37	Y	H	-0.5710
38	W	H	0.0000
39	M	H	0.0000
40	N	H	0.0000
41	W	H	0.0000
42	V	H	0.0000
43	R	H	0.0000
44	Q	H	-0.8688
45	A	H	-1.1436
46	P	H	-0.9808
47	G	H	-1.4661
48	K	H	-2.3024
49	G	H	-1.5758
50	L	H	0.0000
51	E	H	-1.1945
52	W	H	0.0000
53	V	H	0.0000
54	A	H	0.0000
55	N	H	0.0000
56	I	H	0.0000
57	K	H	-1.6809
58	E	H	-2.0072
59	D	H	-2.6539
62	G	H	-2.0065
63	S	H	-1.5094
64	E	H	-1.4947
65	K	H	-1.0947
66	Y	H	-0.5577
67	Y	H	-0.8155
68	V	H	-1.1709
69	D	H	-2.3872
70	S	H	-1.7961
71	V	H	0.0000
72	K	H	-2.5604
74	G	H	-1.7585
75	R	H	-1.7522
76	F	H	0.0000
77	T	H	-0.8483
78	I	H	0.0000
79	S	H	-0.5322
80	R	H	-1.3876
81	D	H	-1.8999
82	N	H	-2.0250
83	A	H	-1.3928
84	K	H	-2.2687
85	N	H	-1.6505
86	S	H	0.0000
87	L	H	0.0000
88	Y	H	-0.4553
89	L	H	0.0000
90	Q	H	-1.0964
91	M	H	0.0000
92	N	H	-1.6740
93	S	H	-1.3390
94	L	H	0.0000
95	R	H	-1.8683
96	V	H	-0.2089
97	E	H	-1.4971
98	D	H	0.0000
99	T	H	-0.2732
100	A	H	0.0000
101	V	H	0.1019
102	Y	H	0.0000
103	Y	H	0.0000
104	C	H	0.0000
105	A	H	0.0000
106	R	H	0.0000
107	L	H	0.0000
108	R	H	-1.5321
109	W	H	0.0522
110	L	H	0.1030
112	R	H	-0.8277
113	G	H	0.0000
114	N	H	0.0000
115	F	H	0.0000
116	D	H	-1.0487
117	H	H	-0.8021
118	W	H	-0.2854
119	G	H	0.0000
120	Q	H	-1.0404
121	G	H	-0.4490
122	T	H	-0.3306
123	L	H	-0.0103
124	V	H	0.0000
125	T	H	-0.2717
126	V	H	0.0000
127	S	H	-0.5379
128	S	H	-0.4044
129	A	H	-0.4999
130	S	H	-0.4565
131	T	H	-0.8329
132	K	H	-0.9448
133	G	H	-1.2399
134	P	H	-0.5707
135	S	H	-0.3357
136	V	H	0.0000
137	F	H	0.0000
138	P	H	-0.6953
139	L	H	0.0000
140	A	H	-0.0904
141	P	H	-0.0602
142	T	H	-0.2244
143	A	H	-0.0979
144	A	H	-0.0539
145	L	H	0.0000
146	G	H	0.0000
147	C	H	0.0000
148	L	H	0.0000
149	V	H	0.0000
150	K	H	-0.3825
151	D	H	-0.5417
152	Y	H	0.0000
153	F	H	-0.1864
154	P	H	0.0000
155	E	H	-0.7712
156	P	H	-0.7800
157	V	H	-0.4161
158	T	H	-0.5289
159	V	H	-0.2867
160	S	H	-0.3315
161	W	H	-0.4405
162	N	H	-1.1179
163	G	H	-0.0571
164	V	H	0.6262
165	H	H	-0.3304
166	T	H	-0.1439
167	F	H	0.0000
168	P	H	-0.1389
169	A	H	0.2175
170	V	H	0.0000
171	L	H	1.1174
172	Q	H	0.2033
173	S	H	-0.1497
174	S	H	-0.2184
175	G	H	0.0846
176	L	H	0.1365
177	Y	H	0.3647
178	S	H	0.0000
179	L	H	0.0000
180	S	H	0.0000
181	S	H	0.0000
182	V	H	0.1498
183	V	H	0.0000
184	T	H	0.0032
185	V	H	-0.0025
186	P	H	-0.2489
187	S	H	-0.3407
188	S	H	-0.4528
189	S	H	-0.4659
190	L	H	-0.0706
191	G	H	-0.6135
192	T	H	-0.7128
193	Q	H	-1.1255
194	T	H	-0.6127
195	Y	H	-0.3392
196	I	H	-0.8390
197	C	H	0.0000
198	N	H	-1.5112
199	V	H	0.0000
200	N	H	-1.7365
201	H	H	0.0000
202	K	H	-2.6963
203	P	H	-1.5509
204	S	H	-1.7022
205	N	H	-2.3473
206	T	H	-1.9597
207	K	H	-2.5989
208	V	H	-1.5707
209	D	H	-2.5184
210	K	H	-1.7720
211	K	H	-1.7755
212	V	H	-0.0207
2	F	L	0.5802
3	M	L	0.9586
4	L	L	0.0000
5	T	L	-0.2491
6	Q	L	-0.7894
7	P	L	-0.9354
8	H	L	-1.5200
9	S	L	-1.1768
11	V	L	-0.5819
12	S	L	-0.3405
13	E	L	-0.8718
14	S	L	-1.0475
15	P	L	-1.6586
16	G	L	-1.9500
17	K	L	-2.3430
18	T	L	-1.3510
19	V	L	0.0000
20	T	L	-0.0932
21	I	L	0.0000
22	S	L	-0.1792
23	C	L	0.0000
24	T	L	-0.1582
25	G	L	0.1027
26	S	L	-0.1105
27	N	L	-0.6564
28	G	L	-0.1381
29	Y	L	0.4367
30	I	L	0.0000
35	A	L	-0.3705
36	N	L	-1.1290
37	N	L	-1.0519
38	Y	L	-0.5719
39	V	L	0.0000
40	Q	L	0.0000
41	W	L	0.0000
42	Y	L	0.0000
43	Q	L	0.0000
44	Q	L	-0.7688
45	R	L	-1.2154
46	P	L	-0.9319
47	G	L	-0.7892
48	S	L	-0.6045
49	V	L	-0.1567
50	P	L	0.0000
51	T	L	-0.1652
52	V	L	0.0000
53	V	L	0.0000
54	I	L	0.0000
55	Y	L	-1.5740
56	E	L	-1.8977
57	D	L	-1.5324
65	N	L	-2.3248
66	Q	L	-2.5830
67	R	L	-2.5551
68	P	L	-1.3725
69	S	L	-1.0132
70	G	L	-0.9390
71	V	L	-0.8956
72	P	L	-1.3749
74	D	L	-2.3255
75	R	L	-1.6027
76	F	L	0.0000
77	S	L	-1.2958
78	G	L	-1.0213
79	S	L	-0.5015
80	I	L	-0.0584
81	D	L	-0.9123
82	S	L	-0.6480
83	S	L	-0.5599
84	S	L	-0.5532
85	N	L	-0.4799
86	S	L	-0.3186
87	A	L	0.0000
88	S	L	-0.2435
89	L	L	0.0000
90	I	L	-0.0801
91	I	L	0.0000
92	S	L	-1.3090
93	G	L	-1.7256
94	L	L	0.0000
95	K	L	-2.6365
96	T	L	-1.6098
97	E	L	-2.1058
98	D	L	0.0000
99	E	L	0.0000
100	A	L	0.0000
101	D	L	-1.2290
102	Y	L	0.0000
103	Y	L	0.0000
104	C	L	0.0000
105	Q	L	0.0000
106	S	L	0.0000
107	Y	L	0.0000
108	D	L	-1.3884
109	T	L	-0.9832
114	P	L	-0.6938
115	N	L	-0.8796
116	V	L	0.0000
117	V	L	-0.0989
118	F	L	0.0000
119	G	L	0.0000
120	G	L	-0.9995
121	G	L	-1.2847
122	T	L	0.0000
123	K	L	-1.8521
124	L	L	0.0000
125	T	L	-0.3611
126	V	L	0.0000
127	L	L	0.4257
128	G	L	-0.4391
129	Q	L	-0.6754
130	P	L	-1.1051
131	K	L	-2.0934
132	A	L	-1.2871
133	A	L	-0.8135
134	P	L	0.0000
135	S	L	-0.1989
136	V	L	-0.3113
137	T	L	-0.2905
138	L	L	0.0000
139	F	L	0.0000
140	P	L	-0.0953
141	P	L	0.0000
142	S	L	0.0000
143	S	L	-1.1868
144	E	L	-2.0262
145	E	L	0.0000
146	L	L	-1.9539
147	Q	L	-2.2462
148	A	L	-1.9239
149	N	L	-2.7785
150	K	L	-2.8937
151	A	L	0.0000
152	T	L	0.0000
153	L	L	0.0000
154	V	L	0.0000
155	C	L	0.0000
156	L	L	0.0000
157	I	L	0.0000
158	S	L	-0.4722
159	D	L	-0.8747
160	F	L	0.0000
161	Y	L	-0.9398
162	P	L	-0.7522
163	G	L	-0.4957
164	A	L	-0.2112
165	V	L	-0.0839
166	T	L	-0.0473
167	V	L	0.1206
168	A	L	-0.3024
169	W	L	0.0000
170	K	L	-1.2129
171	A	L	0.0000
172	D	L	-1.9107
173	S	L	-1.1837
174	S	L	-1.0126
175	P	L	-1.0150
176	V	L	-0.9759
177	K	L	-1.6344
178	A	L	-0.7889
179	G	L	-0.5101
180	V	L	-0.1380
181	E	L	-0.1432
182	T	L	0.0308
183	T	L	0.0000
184	T	L	-0.1808
185	P	L	-0.2522
186	S	L	-0.4742
187	K	L	-1.0310
188	Q	L	-1.1539
189	S	L	-1.2342
190	N	L	-1.3390
191	N	L	0.0000
192	K	L	-1.1376
193	Y	L	-0.6159
194	A	L	0.0000
195	A	L	0.0000
196	S	L	0.0000
197	S	L	0.0000
198	Y	L	0.0000
199	L	L	0.0000
200	S	L	-0.5887
201	L	L	0.0000
202	T	L	-1.8464
203	P	L	-2.5264
204	E	L	-3.0950
205	Q	L	-2.2884
206	W	L	0.0000
207	K	L	-3.0129
208	S	L	-2.3384
209	H	L	-2.4675
210	R	L	-2.7063
211	S	L	0.0000
212	Y	L	0.0000
213	S	L	0.0000
214	C	L	0.0000
215	Q	L	-0.8922
216	V	L	0.0000
217	T	L	-0.6275
218	H	L	0.0000
219	E	L	-2.3085
220	G	L	-1.3636
221	S	L	-0.9522
222	T	L	-0.5920
223	V	L	-0.5265
224	E	L	-1.8283
225	K	L	-1.8532
226	T	L	-1.1251
227	V	L	-0.3938
228	A	L	-0.6687
229	P	L	-0.9639
230	T	L	-0.3229