Project name: 28.D10D10

Chain sequence(s)	H: EVQLVESGGDLVKPGGSLKLSCAASGFTFSSYGMSWVRQTPDKRLEWVATMSTGGSYTYYLNSVKGRFTISRDNAKNILYLQMSSLKSEDTAMYYCTRHRGLPDYNGSRGDYYAMDYWGQGTSVTVSS; L: DIQMTQSPSSLSASLGERVSLTCRASQDIGSSLNWLQQEPDGTIKRLIYATSSLDSGVPKRFSGSRSGSDYSLTISSLESEDFVDYYCLQYASSPYTFGGGTKLEIK
Selected Chain(s)	H,L
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:01:32)
[INFO]       Main:     Simulation completed successfully.                                          (00:01:33)

• Minimal score value: -3.6364
• Maximal score value: 1.1882
• Average score: -0.7056
• Total score value: -165.8125

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	H	-1.9671
2	V	H	-0.8701
3	Q	H	-0.7091
4	L	H	0.0000
5	V	H	1.1882
6	E	H	0.0000
7	S	H	-0.4825
8	G	H	-1.1837
9	G	H	-1.1349
11	D	H	-1.5215
12	L	H	0.2477
13	V	H	-0.7666
14	K	H	-1.9839
15	P	H	-1.7318
16	G	H	-1.4641
17	G	H	-1.0048
18	S	H	-1.0503
19	L	H	-1.1392
20	K	H	-1.8248
21	L	H	0.0000
22	S	H	-0.1733
23	C	H	0.0000
24	A	H	0.1462
25	A	H	0.0000
26	S	H	-0.7811
27	G	H	-1.1173
28	F	H	-0.4542
29	T	H	-0.2355
30	F	H	0.0000
35	S	H	-0.7521
36	S	H	-0.3489
37	Y	H	-0.1785
38	G	H	0.0000
39	M	H	0.0000
40	S	H	0.0000
41	W	H	0.0000
42	V	H	0.0000
43	R	H	0.0000
44	Q	H	0.0000
45	T	H	-2.2187
46	P	H	-2.1576
47	D	H	-3.2625
48	K	H	-3.6364
49	R	H	-3.4589
50	L	H	0.0000
51	E	H	-1.1319
52	W	H	0.0000
53	V	H	0.0000
54	A	H	0.0000
55	T	H	0.0000
56	M	H	0.0000
57	S	H	-0.0537
58	T	H	-0.2944
59	G	H	-0.5812
62	G	H	-0.2878
63	S	H	0.1758
64	Y	H	1.1269
65	T	H	0.7195
66	Y	H	0.3329
67	Y	H	-0.5353
68	L	H	-0.8793
69	N	H	-2.0078
70	S	H	-1.6347
71	V	H	0.0000
72	K	H	-2.3101
74	G	H	-1.6076
75	R	H	-1.4519
76	F	H	0.0000
77	T	H	-0.8449
78	I	H	0.0000
79	S	H	-0.2852
80	R	H	-0.8157
81	D	H	-1.2231
82	N	H	-1.5609
83	A	H	-1.2438
84	K	H	-2.1327
85	N	H	-1.5758
86	I	H	-0.4690
87	L	H	0.0000
88	Y	H	-0.3573
89	L	H	0.0000
90	Q	H	-1.3975
91	M	H	0.0000
92	S	H	-1.0541
93	S	H	-1.1341
94	L	H	0.0000
95	K	H	-2.3072
96	S	H	-1.9519
97	E	H	-2.3700
98	D	H	0.0000
99	T	H	-0.6908
100	A	H	0.0000
101	M	H	-0.2852
102	Y	H	0.0000
103	Y	H	0.0000
104	C	H	0.0000
105	T	H	0.0000
106	R	H	0.0000
107	H	H	0.0000
108	R	H	-0.5280
109	G	H	-1.0894
110	L	H	-0.5028
111	P	H	-0.7940
111A	D	H	-1.3125
111B	Y	H	-0.1596
111C	N	H	-1.3546
111D	G	H	-1.3764
112D	S	H	-1.5204
112C	R	H	-2.6079
112B	G	H	-1.8835
112A	D	H	-2.2532
112	Y	H	-0.9968
113	Y	H	-0.4248
114	A	H	0.0000
115	M	H	0.0000
116	D	H	0.0061
117	Y	H	0.5340
118	W	H	0.0000
119	G	H	0.0000
120	Q	H	-1.1713
121	G	H	-0.3645
122	T	H	-0.4880
123	S	H	-0.5547
124	V	H	0.0000
125	T	H	-0.6277
126	V	H	0.0000
127	S	H	-0.9196
128	S	H	-0.7163
1	D	L	-2.4614
2	I	L	0.0000
3	Q	L	-2.2995
4	M	L	0.0000
5	T	L	-1.3841
6	Q	L	-1.1024
7	S	L	-0.7333
8	P	L	-0.5283
9	S	L	-0.6969
10	S	L	-0.8592
11	L	L	-0.5036
12	S	L	-0.6544
13	A	L	0.0000
14	S	L	-0.6523
15	L	L	0.2399
16	G	L	-1.2472
17	E	L	-2.4726
18	R	L	-2.5626
19	V	L	0.0000
20	S	L	-0.6086
21	L	L	0.0000
22	T	L	-0.8247
23	C	L	0.0000
24	R	L	-2.8944
25	A	L	0.0000
26	S	L	-2.3130
27	Q	L	-2.8643
28	D	L	-2.8318
29	I	L	0.0000
36	G	L	-1.0882
37	S	L	-0.7325
38	S	L	-0.3312
39	L	L	0.0000
40	N	L	0.0000
41	W	L	0.0000
42	L	L	0.0000
43	Q	L	0.0000
44	Q	L	0.0000
45	E	L	-1.4013
46	P	L	-1.3886
47	D	L	-2.2023
48	G	L	-1.6388
49	T	L	-1.1376
50	I	L	0.0000
51	K	L	-0.5018
52	R	L	0.0000
53	L	L	0.0000
54	I	L	0.0000
55	Y	L	0.0642
56	A	L	-0.0502
57	T	L	0.0000
65	S	L	-0.4040
66	S	L	-0.0635
67	L	L	-0.2117
68	D	L	-0.3410
69	S	L	-0.4815
70	G	L	-0.7984
71	V	L	0.0000
72	P	L	-1.1125
74	K	L	-1.8827
75	R	L	-1.5428
76	F	L	0.0000
77	S	L	-0.6254
78	G	L	-0.4188
79	S	L	-0.8350
80	R	L	-1.5218
83	S	L	-1.1991
84	G	L	-1.3785
85	S	L	-1.9961
86	D	L	-1.8943
87	Y	L	0.0000
88	S	L	-0.8185
89	L	L	0.0000
90	T	L	-0.6364
91	I	L	0.0000
92	S	L	-1.6780
93	S	L	-1.5894
94	L	L	0.0000
95	E	L	-1.5791
96	S	L	-1.2117
97	E	L	-2.2792
98	D	L	0.0000
99	F	L	-0.7512
100	V	L	0.0000
101	D	L	-1.1386
102	Y	L	0.0000
103	Y	L	0.0000
104	C	L	0.0000
105	L	L	0.0000
106	Q	L	0.0000
107	Y	L	0.0000
108	A	L	-0.5812
109	S	L	-0.4854
114	S	L	-0.5962
115	P	L	-0.7621
116	Y	L	0.0000
117	T	L	-0.7670
118	F	L	0.0000
119	G	L	0.0000
120	G	L	-1.4953
121	G	L	0.0000
122	T	L	0.0000
123	K	L	-1.3789
124	L	L	0.0000
125	E	L	-1.0937
126	I	L	0.6446
127	K	L	-0.9921