Project name: obj1 [mutate: SR25C, QN39C, GD44C, LH45C]

Chain sequence(s)	C: EVQLVESGGGLVQPGGSLRLSCAASDFTFRSYEMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARLRDGFNKGFDYWGQGTLVTVSS
Selected Chain(s)	C
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No
Mutated residues	GD44C,LH45C,QN39C,SR25C
Energy difference between WT (input) and mutated protein (by FoldX)	-0.164358 kcal/mol

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with C chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       FoldX:    Building mutant model                                                       (00:00:24)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:41)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:01:06)
[INFO]       Main:     Simulation completed successfully.                                          (00:01:06)

• Minimal score value: -3.3296
• Maximal score value: 1.8377
• Average score: -0.7782
• Total score value: -93.3893

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	C	-2.2237
2	V	C	-1.4012
3	Q	C	-1.8203
4	L	C	0.0000
5	V	C	0.1825
6	E	C	0.1372
7	S	C	-0.4460
8	G	C	-0.8149
9	G	C	0.0276
10	G	C	0.9366
11	L	C	1.4406
12	V	C	-0.0385
13	Q	C	-1.3371
14	P	C	-1.4887
15	G	C	-1.4134
16	G	C	-0.9785
17	S	C	-1.2309
18	L	C	-0.9630
19	R	C	-2.1462
20	L	C	0.0000
21	S	C	-0.5691
22	C	C	0.0000
23	A	C	-0.6495
24	A	C	0.0000
25	R	C	-1.8559	mutated: SR25C
26	D	C	0.0000
27	F	C	1.1474
28	T	C	0.0410
29	F	C	0.0000
30	R	C	-2.0325
31	S	C	-0.8863
32	Y	C	-1.2160
33	E	C	-1.1424
34	M	C	0.0000
35	S	C	0.0000
36	W	C	0.0000
37	V	C	0.0000
38	R	C	0.0000
39	N	C	-1.2549	mutated: QN39C
40	A	C	-1.5427
41	P	C	-1.4346
42	G	C	-1.8941
43	K	C	-3.1806
44	D	C	-3.3078	mutated: GD44C
45	H	C	-2.1825	mutated: LH45C
46	E	C	-1.6380
47	W	C	-0.0967
48	V	C	0.0000
49	S	C	0.0000
50	A	C	0.0000
51	I	C	0.0000
52	S	C	-0.5831
53	G	C	-1.2447
54	S	C	-1.2286
55	G	C	-1.0817
56	G	C	-0.7345
57	S	C	-0.3025
58	T	C	0.1989
59	Y	C	0.6077
60	Y	C	-0.3558
61	A	C	-1.1372
62	D	C	-2.3439
63	S	C	-1.7142
64	V	C	0.0000
65	K	C	-2.3844
66	G	C	-1.6174
67	R	C	0.0000
68	F	C	0.0000
69	T	C	-0.6722
70	I	C	0.0000
71	S	C	-0.5624
72	R	C	-1.3626
73	D	C	-1.9805
74	N	C	-2.1901
75	S	C	-1.7905
76	K	C	-2.3193
77	N	C	-1.9949
78	T	C	0.0000
79	L	C	0.0000
80	Y	C	-0.6718
81	L	C	0.0000
82	Q	C	-1.2337
83	M	C	0.0000
84	N	C	-1.3327
85	S	C	-1.2322
86	L	C	0.0000
87	R	C	-2.4721
88	A	C	-1.8894
89	E	C	-2.3444
90	D	C	0.0000
91	T	C	-0.3727
92	A	C	0.0000
93	I	C	1.0106
94	Y	C	0.0000
95	Y	C	0.4048
96	C	C	0.0000
97	A	C	0.0000
98	R	C	0.0000
99	L	C	0.0000
100	R	C	-3.1827
101	D	C	-3.3296
102	G	C	-2.0833
103	F	C	-1.2274
104	N	C	-2.4248
105	K	C	-3.1791
106	G	C	-1.8945
107	F	C	-0.9752
108	D	C	-1.1083
109	Y	C	-0.2406
110	W	C	0.4633
111	G	C	-0.1598
112	Q	C	-0.9227
113	G	C	0.1184
114	T	C	0.5998
115	L	C	1.8377
116	V	C	0.0000
117	T	C	0.3552
118	V	C	0.0000
119	S	C	-0.7749
120	S	C	-1.0602