Project name: obj1 [mutate: YK59C, YP60C, AV61C, KY65C]

Chain sequence(s)	C: EVQLVESGGGLVQPGGSLRLSCAASDFTFRSYEMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARLRDGFNKGFDYWGQGTLVTVSS
Selected Chain(s)	C
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No
Mutated residues	KY65C,YP60C,YK59C,AV61C
Energy difference between WT (input) and mutated protein (by FoldX)	5.7578 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with C chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       FoldX:    Building mutant model                                                       (00:00:26)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:01:39)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:02:04)
[INFO]       Main:     Simulation completed successfully.                                          (00:02:05)

• Minimal score value: -3.284
• Maximal score value: 1.7651
• Average score: -0.6432
• Total score value: -77.1876

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	C	-2.0063
2	V	C	-0.9165
3	Q	C	-1.2443
4	L	C	0.0000
5	V	C	0.5648
6	E	C	0.2212
7	S	C	-0.4097
8	G	C	-0.8149
9	G	C	0.0110
10	G	C	0.9239
11	L	C	1.4406
12	V	C	-0.0388
13	Q	C	-1.3373
14	P	C	-1.4890
15	G	C	-1.4138
16	G	C	-0.9793
17	S	C	-1.2326
18	L	C	-0.9638
19	R	C	-2.1483
20	L	C	0.0000
21	S	C	-0.5419
22	C	C	0.0000
23	A	C	-0.2017
24	A	C	0.0000
25	S	C	-0.2017
26	D	C	0.0000
27	F	C	1.5458
28	T	C	0.2525
29	F	C	0.0000
30	R	C	-2.0321
31	S	C	-0.8846
32	Y	C	-1.2113
33	E	C	-1.1093
34	M	C	0.0000
35	S	C	0.0000
36	W	C	0.0000
37	V	C	0.0000
38	R	C	0.0000
39	Q	C	-0.2819
40	A	C	-0.9666
41	P	C	-1.2936
42	G	C	-1.4347
43	K	C	-2.1190
44	G	C	-1.0327
45	L	C	0.4245
46	E	C	-0.2971
47	W	C	0.3101
48	V	C	0.0000
49	S	C	0.0000
50	A	C	0.0000
51	I	C	0.0000
52	S	C	-0.9224
53	G	C	-1.2599
54	S	C	-1.2498
55	G	C	-1.1280
56	G	C	-0.7960
57	S	C	-0.9968
58	T	C	-1.2062
59	K	C	-1.6814	mutated: YK59C
60	P	C	-0.8432	mutated: YP60C
61	V	C	-0.7459	mutated: AV61C
62	D	C	-1.5593
63	S	C	-0.8489
64	V	C	0.0000
65	Y	C	0.2599	mutated: KY65C
66	G	C	-0.1634
67	R	C	0.0000
68	F	C	0.0000
69	T	C	-0.5793
70	I	C	0.0000
71	S	C	-0.5845
72	R	C	-1.3656
73	D	C	-1.9820
74	N	C	-2.1912
75	S	C	-1.7905
76	K	C	-2.3163
77	N	C	-1.6491
78	T	C	0.0000
79	L	C	0.0000
80	Y	C	-0.6724
81	L	C	0.0000
82	Q	C	-1.2396
83	M	C	0.0000
84	N	C	-1.3356
85	S	C	-1.2327
86	L	C	0.0000
87	R	C	-2.4723
88	A	C	-1.8894
89	E	C	-2.3441
90	D	C	0.0000
91	T	C	-0.4216
92	A	C	0.0000
93	I	C	1.0770
94	Y	C	0.0000
95	Y	C	0.6338
96	C	C	0.0000
97	A	C	0.0000
98	R	C	0.0000
99	L	C	0.0000
100	R	C	-3.1556
101	D	C	-3.2840
102	G	C	-1.9779
103	F	C	-1.3397
104	N	C	-2.3262
105	K	C	-3.1281
106	G	C	-1.8650
107	F	C	-0.9579
108	D	C	-1.0994
109	Y	C	-0.2104
110	W	C	0.5562
111	G	C	-0.0702
112	Q	C	-0.8792
113	G	C	0.1067
114	T	C	0.5812
115	L	C	1.7651
116	V	C	0.0000
117	T	C	0.3370
118	V	C	0.0000
119	S	C	-0.7749
120	S	C	-1.0602