Project name: VHL-12VC1.2

Chain sequence(s)	A: MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQRMGDSSSSGVSSVPTELEVVAATPTSLLISWDAPAVTVFFYVITYGETGHGVGAFQAFAVPGSRSTATISGLEPGVDYTITVYARGYSKQGPYKPSPISINYRT
Selected Chain(s)	A
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with A chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:03:53)
[INFO]       Main:     Simulation completed successfully.                                          (00:03:56)

• Minimal score value: -3.9411
• Maximal score value: 1.946
• Average score: -0.9824
• Total score value: -307.496

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	M	A	0.3151
2	P	A	-1.0487
3	R	A	-2.6092
4	R	A	-3.0475
5	A	A	-2.4154
6	E	A	-2.6957
7	N	A	-1.9829
8	W	A	-1.1147
9	D	A	-2.7381
10	E	A	-2.6449
11	A	A	-1.5851
12	E	A	-1.6730
13	V	A	0.5025
14	G	A	-0.4283
15	A	A	-1.1111
16	E	A	-2.7916
17	E	A	-2.7747
18	A	A	-1.0466
19	G	A	-0.2990
20	V	A	0.3058
21	E	A	-1.7844
22	E	A	-1.8982
23	Y	A	-0.1986
24	G	A	-0.9573
25	P	A	-1.6643
26	E	A	-3.4735
27	E	A	-3.8704
28	D	A	-3.7437
29	G	A	-2.8650
30	G	A	-2.7291
31	E	A	-3.3893
32	E	A	-3.1243
33	S	A	-2.1639
34	G	A	-2.3627
35	A	A	-2.0766
36	E	A	-3.1468
37	E	A	-2.7192
38	S	A	-1.9791
39	G	A	-1.8636
40	P	A	-1.7286
41	E	A	-2.8978
42	E	A	-3.0039
43	S	A	-2.0786
44	G	A	-2.2384
45	P	A	-1.8135
46	E	A	-2.6260
47	E	A	-2.1664
48	L	A	-0.3667
49	G	A	-1.0479
50	A	A	-1.6943
51	E	A	-3.0871
52	E	A	-3.6635
53	E	A	-3.1400
54	M	A	-1.4327
55	E	A	-2.5448
56	A	A	-1.4310
57	G	A	-1.8475
58	R	A	-2.9505
59	P	A	-2.1893
60	R	A	-2.6693
61	P	A	-1.6877
62	V	A	-0.1518
63	L	A	0.0000
64	R	A	-1.1872
65	S	A	0.0000
66	V	A	0.4691
67	N	A	-0.8553
68	S	A	-1.2773
69	R	A	-2.3453
70	E	A	-1.6410
71	P	A	-1.0436
72	S	A	0.0000
73	Q	A	-0.9277
74	V	A	0.0000
75	I	A	-0.3391
76	F	A	0.0000
77	C	A	-0.8940
78	N	A	0.0000
79	R	A	-2.2132
80	S	A	0.0000
81	P	A	-0.8707
82	R	A	-0.7634
83	V	A	0.2162
84	V	A	0.0000
85	L	A	0.0389
86	P	A	0.0000
87	V	A	0.0000
88	W	A	0.0000
89	L	A	0.0000
90	N	A	-1.0962
91	F	A	-0.6284
92	D	A	-2.3134
93	G	A	-1.9559
94	E	A	-2.7749
95	P	A	-1.6973
96	Q	A	-1.1491
97	P	A	-0.6485
98	Y	A	-0.1679
99	P	A	-0.2987
100	T	A	0.0554
101	L	A	0.0000
102	P	A	-0.5287
103	P	A	-1.0423
104	G	A	-1.3045
105	T	A	-0.8745
106	G	A	-1.2034
107	R	A	-1.7708
108	R	A	-2.6240
109	I	A	0.0000
110	H	A	-1.5580
111	S	A	0.0000
112	Y	A	-0.5452
113	R	A	-0.5085
114	G	A	-0.0019
115	H	A	0.0000
116	L	A	0.4423
117	W	A	0.0000
118	L	A	0.0000
119	F	A	0.0000
120	R	A	0.0000
121	D	A	0.0000
122	A	A	-0.2359
123	G	A	-0.5276
124	T	A	-0.7354
125	H	A	-0.8128
126	D	A	0.0000
127	G	A	-0.3176
128	L	A	0.0000
129	L	A	-0.0778
130	V	A	0.0000
131	N	A	-0.7718
132	Q	A	-1.3900
133	T	A	-0.7424
134	E	A	-0.8185
135	L	A	0.0344
136	F	A	0.0000
137	V	A	0.7734
138	P	A	0.0000
139	S	A	0.5579
140	L	A	0.8243
141	N	A	-0.3052
142	V	A	-0.3589
143	D	A	-2.0440
144	G	A	-1.5171
145	Q	A	-1.3266
146	P	A	-0.6364
147	I	A	0.5931
148	F	A	0.7086
149	A	A	0.0000
150	N	A	-0.6039
151	I	A	0.0000
152	T	A	-0.4941
153	L	A	0.4974
154	P	A	0.9844
155	V	A	1.9460
156	Y	A	0.6088
157	T	A	0.0151
158	L	A	0.2985
159	K	A	-1.7008
160	E	A	-1.6069
161	R	A	-1.3271
162	C	A	-0.4714
163	L	A	0.0000
164	Q	A	-0.3704
165	V	A	0.7456
166	V	A	0.6244
167	R	A	-0.2254
168	S	A	0.4127
169	L	A	1.3649
170	V	A	-0.3345
171	K	A	-2.0736
172	P	A	-2.5857
173	E	A	-3.3713
174	N	A	-2.9252
175	Y	A	0.0000
176	R	A	-3.9411
177	R	A	-3.3054
178	L	A	-1.7539
179	D	A	-1.9686
180	I	A	-0.2321
181	V	A	0.8946
182	R	A	-1.6922
183	S	A	-0.9410
184	L	A	-1.1999
185	Y	A	0.0000
186	E	A	-3.4887
187	D	A	-2.8382
188	L	A	0.0000
189	E	A	-3.4010
190	D	A	-3.2988
191	H	A	-2.4593
192	P	A	-1.7549
193	N	A	-2.0818
194	V	A	-1.9955
195	Q	A	-2.9886
196	K	A	-3.2684
197	D	A	-2.5829
198	L	A	0.0000
199	E	A	-3.3426
200	R	A	-2.7381
201	L	A	0.0000
202	T	A	-1.5182
203	Q	A	-2.0138
204	E	A	-2.1135
205	R	A	-1.6536
206	I	A	-1.0145
207	A	A	-1.4829
208	H	A	-2.0969
209	Q	A	-2.9353
210	R	A	-2.9012
211	M	A	-1.1929
212	G	A	-2.1103
213	D	A	-3.0621
214	S	A	-1.5960
215	S	A	-1.0609
216	S	A	-0.9711
217	S	A	-0.6298
218	G	A	-0.2812
219	V	A	0.3410
220	S	A	0.1897
221	S	A	0.0000
222	V	A	0.1233
223	P	A	0.0000
224	T	A	-1.6156
225	E	A	-2.7329
226	L	A	0.0000
227	E	A	-1.9250
228	V	A	0.1414
229	V	A	1.5645
230	A	A	0.9128
231	A	A	0.2996
232	T	A	-0.1756
233	P	A	-1.0993
234	T	A	-1.0045
235	S	A	-0.5319
236	L	A	0.0000
237	L	A	0.8036
238	I	A	0.0000
239	S	A	-0.9220
240	W	A	0.0000
241	D	A	-2.6322
242	A	A	-1.0868
243	P	A	0.0299
244	A	A	0.2348
245	V	A	0.6956
246	T	A	0.3146
247	V	A	0.9103
248	F	A	1.4480
249	F	A	0.8067
250	Y	A	0.0000
251	V	A	0.4735
252	I	A	0.0000
253	T	A	0.1243
254	Y	A	-0.1606
255	G	A	0.0000
256	E	A	-1.8859
257	T	A	-1.4912
258	G	A	-1.2177
259	H	A	-1.2714
260	G	A	-0.3931
261	V	A	0.9375
262	G	A	0.0041
263	A	A	-0.3230
264	F	A	-0.1551
265	Q	A	-0.9537
266	A	A	-0.2528
267	F	A	0.3897
268	A	A	0.7620
269	V	A	0.1271
270	P	A	-0.2242
271	G	A	-0.0025
272	S	A	-0.7021
273	R	A	-1.3553
274	S	A	-1.1606
275	T	A	-0.5142
276	A	A	0.0000
277	T	A	0.1079
278	I	A	0.0000
279	S	A	-0.6571
280	G	A	-1.0623
281	L	A	0.0000
282	E	A	-2.5236
283	P	A	-1.7141
284	G	A	-1.3802
285	V	A	-1.6370
286	D	A	-2.7209
287	Y	A	0.0000
288	T	A	-1.2249
289	I	A	0.0000
290	T	A	0.0258
291	V	A	0.0000
292	Y	A	0.2321
293	A	A	0.0000
294	R	A	0.3526
295	G	A	0.0000
296	Y	A	0.3487
297	S	A	-1.1746
298	K	A	-2.5909
299	Q	A	-1.9091
300	G	A	-1.1589
301	P	A	-0.7679
302	Y	A	0.0000
303	K	A	-1.6497
304	P	A	-0.8365
305	S	A	-0.3849
306	P	A	-0.0461
307	I	A	0.1409
308	S	A	-0.5166
309	I	A	-0.7123
310	N	A	-1.9131
311	Y	A	-1.6454
312	R	A	-2.7793
313	T	A	-1.6336