Project name: obj1 [mutate: EA46C, QM3C, MT83C, YV80C, YF94C]

Chain sequence(s)	C: EVQLVESGGGLVQPGGSLRLSCAASDFTFRSYEMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARLRDGFNKGFDYWGQGTLVTVSS
Selected Chain(s)	C
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No
Mutated residues	QM3C,YV80C,EA46C,MT83C,YF94C
Energy difference between WT (input) and mutated protein (by FoldX)	9.43757 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with C chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       FoldX:    Building mutant model                                                       (00:00:24)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:01:05)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:01:32)
[INFO]       Main:     Simulation completed successfully.                                          (00:01:32)

• Minimal score value: -3.3333
• Maximal score value: 1.7391
• Average score: -0.5824
• Total score value: -69.8877

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	C	-1.5532
2	V	C	0.0931
3	M	C	0.8153	mutated: QM3C
4	L	C	0.0000
5	V	C	0.9308
6	E	C	0.1725
7	S	C	-0.4369
8	G	C	-0.8031
9	G	C	0.0142
10	G	C	0.9265
11	L	C	1.4423
12	V	C	-0.0373
13	Q	C	-1.3376
14	P	C	-1.4895
15	G	C	-1.4147
16	G	C	-0.9796
17	S	C	-1.2010
18	L	C	-0.9119
19	R	C	-2.0430
20	L	C	0.0000
21	S	C	-0.5070
22	C	C	0.0000
23	A	C	0.1014
24	A	C	0.0000
25	S	C	0.4554
26	D	C	0.0000
27	F	C	1.5462
28	T	C	0.2527
29	F	C	0.0000
30	R	C	-2.0068
31	S	C	-0.8863
32	Y	C	-1.2160
33	E	C	-1.1454
34	M	C	0.0000
35	S	C	0.0000
36	W	C	0.0000
37	V	C	0.0000
38	R	C	0.2652
39	Q	C	-0.1843
40	A	C	-0.9062
41	P	C	-1.3171
42	G	C	-1.4351
43	K	C	-2.0365
44	G	C	-0.8869
45	L	C	0.7520
46	A	C	0.4143	mutated: EA46C
47	W	C	0.7592
48	V	C	0.0000
49	S	C	0.0000
50	A	C	0.0000
51	I	C	0.0000
52	S	C	-0.5839
53	G	C	-1.2251
54	S	C	-1.2010
55	G	C	-1.0537
56	G	C	-0.7351
57	S	C	-0.3023
58	T	C	0.1987
59	Y	C	0.6083
60	Y	C	-0.3537
61	A	C	-1.0281
62	D	C	-2.3675
63	S	C	-1.7649
64	V	C	0.0000
65	K	C	-2.3978
66	G	C	-1.6240
67	R	C	0.0000
68	F	C	0.0000
69	T	C	-0.5860
70	I	C	0.0000
71	S	C	-0.4464
72	R	C	-1.2303
73	D	C	-1.7598
74	N	C	-2.0977
75	S	C	-1.7293
76	K	C	-2.2589
77	N	C	-1.6028
78	T	C	-1.1498
79	L	C	0.0000
80	V	C	-0.4484	mutated: YV80C
81	L	C	0.0000
82	Q	C	-1.0296
83	T	C	0.0000	mutated: MT83C
84	N	C	-1.2955
85	S	C	-1.2336
86	L	C	0.0000
87	R	C	-2.4728
88	A	C	-1.8894
89	E	C	-2.3503
90	D	C	0.0000
91	T	C	-0.4527
92	A	C	0.0000
93	I	C	1.0072
94	F	C	0.0000	mutated: YF94C
95	Y	C	0.5764
96	C	C	0.0000
97	A	C	0.0000
98	R	C	0.0000
99	L	C	0.0000
100	R	C	-3.1907
101	D	C	-3.3333
102	G	C	-2.0866
103	F	C	-1.2310
104	N	C	-2.4286
105	K	C	-3.1848
106	G	C	-1.9076
107	F	C	-0.9859
108	D	C	-1.0992
109	Y	C	0.1935
110	W	C	0.9069
111	G	C	0.3476
112	Q	C	-0.6471
113	G	C	0.0546
114	T	C	0.5368
115	L	C	1.7391
116	V	C	0.0000
117	T	C	0.3378
118	V	C	0.0000
119	S	C	-0.7749
120	S	C	-1.0602