Project name: L131Q [mutate: LQ131A]

Chain sequence(s)	A: MASHRLLLLCLAGLVFVSEAGPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEQVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE
Selected Chain(s)	A
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No
Mutated residues	LQ131A
Energy difference between WT (input) and mutated protein (by FoldX)	3.08582 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with A chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       FoldX:    Building mutant model                                                       (00:01:34)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:01:41)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:02:24)
[INFO]       Main:     Simulation completed successfully.                                          (00:02:25)

• Minimal score value: -3.2523
• Maximal score value: 3.8652
• Average score: -0.4098
• Total score value: -60.2348

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	M	A	0.5176
2	A	A	-0.1152
3	S	A	-0.1132
4	H	A	-0.3530
5	R	A	-0.2029
6	L	A	2.2157
7	L	A	2.8770
8	L	A	3.1142
9	L	A	3.5138
10	C	A	3.3529
11	L	A	3.7841
12	A	A	3.4618
13	G	A	2.6924
14	L	A	3.7431
15	V	A	3.8652
16	F	A	3.2857
17	V	A	2.7997
18	S	A	0.3798
19	E	A	-1.2790
20	A	A	-1.0428
21	G	A	-1.2635
22	P	A	-1.2296
23	T	A	-1.1230
24	G	A	-1.2510
25	T	A	-1.0809
26	G	A	-1.9042
27	E	A	-2.9031
28	S	A	-2.2204
29	K	A	-2.9411
30	C	A	0.0000
31	P	A	0.0000
32	L	A	0.0000
33	M	A	-0.4759
34	V	A	0.0000
35	K	A	-0.5095
36	V	A	0.0000
37	L	A	0.4444
38	D	A	0.0000
39	A	A	0.6467
40	V	A	1.1655
41	R	A	-0.5936
42	G	A	-0.4190
43	S	A	-0.3903
44	P	A	-0.7176
45	A	A	0.0000
46	I	A	-1.0206
47	N	A	-2.0539
48	V	A	0.0000
49	A	A	-1.4448
50	V	A	0.0000
51	H	A	-0.9459
52	V	A	0.0000
53	F	A	-0.1485
54	R	A	-0.5240
55	K	A	-1.2052
56	A	A	-1.4790
57	A	A	-1.4737
58	D	A	-2.6601
59	D	A	-2.7564
60	T	A	-1.6236
61	W	A	-0.7996
62	E	A	-0.9902
63	P	A	-0.5231
64	F	A	-0.3232
65	A	A	-0.2457
66	S	A	-0.7727
67	G	A	-1.4683
68	K	A	-2.5697
69	T	A	0.0000
70	S	A	-1.8738
71	E	A	-2.2175
72	S	A	-1.2454
73	G	A	0.0000
74	E	A	-1.4573
75	L	A	0.0000
76	H	A	-1.7215
77	G	A	-1.3485
78	L	A	0.0000
79	T	A	0.0000
80	T	A	-2.2457
81	E	A	-3.1996
82	E	A	-3.0733
83	E	A	-2.7840
84	F	A	-1.7559
85	V	A	-0.9080
86	E	A	-2.1564
87	G	A	-0.8636
88	I	A	0.2227
89	Y	A	0.0000
90	K	A	-0.0518
91	V	A	0.0000
92	E	A	-1.2000
93	I	A	0.0000
94	D	A	-1.9569
95	T	A	0.0000
96	K	A	-1.7698
97	S	A	-1.4791
98	Y	A	0.0000
99	W	A	0.0000
100	K	A	-1.4011
101	A	A	-0.4462
102	L	A	0.1614
103	G	A	-0.3663
104	I	A	0.0896
105	S	A	-0.1285
106	P	A	0.2505
107	F	A	1.6071
108	H	A	-0.3426
109	E	A	-2.2826
110	H	A	-1.8319
111	A	A	0.0000
112	E	A	-1.2180
113	Q	A	-0.2442
114	V	A	0.7772
115	F	A	0.5376
116	T	A	-0.4290
117	A	A	0.0000
118	N	A	-2.4930
119	D	A	-2.9463
120	S	A	-2.1689
121	G	A	-2.0427
122	P	A	-2.4088
123	R	A	-2.1616
124	R	A	-1.0761
125	Y	A	0.0000
126	T	A	0.4426
127	I	A	0.0000
128	A	A	0.1924
129	A	A	0.0000
130	L	A	1.0072
131	Q	A	0.0000	mutated: LQ131A
132	S	A	1.1510
133	P	A	0.0000
134	Y	A	1.8542
135	S	A	0.9672
136	Y	A	0.5136
137	S	A	0.0909
138	T	A	0.1564
139	T	A	0.3896
140	A	A	1.0128
141	V	A	1.8454
142	V	A	1.1500
143	T	A	-0.6564
144	N	A	-2.6463
145	P	A	-2.5301
146	K	A	-3.2523
147	E	A	-2.9758