Project name: FEFEFEGGGKGH20

Chain sequence(s)	A: FEFEFEGGGKGH; C: FEFEFEGGGKGH; B: FEFEFEGGGKGH; E: FEFEFEGGGKGH; D: FEFEFEGGGKGH; G: FEFEFEGGGKGH; F: FEFEFEGGGKGH; I: FEFEFEGGGKGH; H: FEFEFEGGGKGH; K: FEFEFEGGGKGH; J: FEFEFEGGGKGH; M: FEFEFEGGGKGH; L: FEFEFEGGGKGH; O: FEFEFEGGGKGH; N: FEFEFEGGGKGH; Q: FEFEFEGGGKGH; P: FEFEFEGGGKGH; S: FEFEFEGGGKGH; R: FEFEFEGGGKGH; T: FEFEFEGGGKGH
Selected Chain(s)	A,C,B,E,D,G,F,I,H,K,J,M,L,O,N,Q,P,S,R,T
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:01)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:01)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:01)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:01)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:01)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:14:45)
[INFO]       Main:     Simulation completed successfully.                                          (00:14:51)

• Minimal score value: -4.9815
• Maximal score value: 1.901
• Average score: -2.0659
• Total score value: -495.8276

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	F	A	1.7621
2	E	A	0.0000
3	F	A	0.5528
4	E	A	0.0000
5	F	A	0.0604
6	E	A	0.0000
7	G	A	-1.3427
8	G	A	-2.0694
9	G	A	-1.9404
10	K	A	-2.8639
11	G	A	-2.6654
12	H	A	-2.6010
1	F	B	1.9010
2	E	B	0.1338
3	F	B	-0.1353
4	E	B	0.0000
5	F	B	-1.6309
6	E	B	-3.0155
7	G	B	0.0000
8	G	B	-2.2799
9	G	B	-2.4346
10	K	B	-2.9885
11	G	B	-2.6574
12	H	B	-2.6535
1	F	C	1.2971
2	E	C	-0.4971
3	F	C	-1.9211
4	E	C	-4.2178
5	F	C	0.0000
6	E	C	-4.4184
7	G	C	0.0000
8	G	C	-2.1684
9	G	C	-2.2368
10	K	C	-2.2248
11	G	C	-1.9274
12	H	C	-2.0099
1	F	D	0.1621
2	E	D	-2.3044
3	F	D	0.0000
4	E	D	-4.3531
5	F	D	-3.6793
6	E	D	-4.1415
7	G	D	-2.4859
8	G	D	-2.2766
9	G	D	-2.0972
10	K	D	-2.2987
11	G	D	-2.0509
12	H	D	-1.9180
1	F	E	-0.0045
2	E	E	-2.7043
3	F	E	-2.3628
4	E	E	-4.3092
5	F	E	-3.2529
6	E	E	-4.1264
7	G	E	-2.4740
8	G	E	-2.3018
9	G	E	-2.3271
10	K	E	-2.9619
11	G	E	-2.3767
12	H	E	-2.1065
1	F	F	0.8519
2	E	F	-2.0142
3	F	F	-1.7638
4	E	F	-4.0523
5	F	F	-3.2055
6	E	F	-4.1925
7	G	F	-2.6592
8	G	F	-2.6094
9	G	F	-2.3864
10	K	F	-2.7593
11	G	F	-2.6054
12	H	F	-2.4021
1	F	G	1.2016
2	E	G	-1.3583
3	F	G	-1.6083
4	E	G	-4.0032
5	F	G	-3.1048
6	E	G	-4.3598
7	G	G	-2.6997
8	G	G	-2.6221
9	G	G	-2.4669
10	K	G	-3.0774
11	G	G	-2.6030
12	H	G	-2.4203
1	F	H	0.7638
2	E	H	-2.0611
3	F	H	-1.8753
4	E	H	-4.1305
5	F	H	-3.1928
6	E	H	-4.4035
7	G	H	-2.6706
8	G	H	-2.6268
9	G	H	-2.2733
10	K	H	-2.8666
11	G	H	-2.6377
12	H	H	-2.3041
1	F	I	-0.0069
2	E	I	-2.6130
3	F	I	-2.2679
4	E	I	-4.2521
5	F	I	-3.1528
6	E	I	-4.1872
7	G	I	-2.5758
8	G	I	-2.5036
9	G	I	-2.2475
10	K	I	-2.8300
11	G	I	-2.5709
12	H	I	-2.1840
1	F	J	-0.0282
2	E	J	-2.6903
3	F	J	-2.2906
4	E	J	-4.1118
5	F	J	-3.0606
6	E	J	-4.0380
7	G	J	-2.4740
8	G	J	-2.2600
9	G	J	-2.1291
10	K	J	-2.2071
11	G	J	-2.3564
12	H	J	-2.0690
1	F	K	0.1811
2	E	K	-2.5100
3	F	K	-2.0620
4	E	K	-3.8028
5	F	K	-2.6480
6	E	K	-3.7521
7	G	K	-2.1180
8	G	K	-2.2543
9	G	K	-2.0502
10	K	K	-2.3467
11	G	K	-2.3797
12	H	K	-2.1827
1	F	L	0.3607
2	E	L	-2.3141
3	F	L	-1.9249
4	E	L	-3.8045
5	F	L	-2.2917
6	E	L	-3.6819
7	G	L	-2.0623
8	G	L	-2.3950
9	G	L	-2.2556
10	K	L	-2.9981
11	G	L	-2.6418
12	H	L	-2.2744
1	F	M	0.3738
2	E	M	-2.1982
3	F	M	-1.8850
4	E	M	-3.8553
5	F	M	-2.3247
6	E	M	-3.6738
7	G	M	-2.0738
8	G	M	-2.3316
9	G	M	-2.2939
10	K	M	-2.7016
11	G	M	-2.6482
12	H	M	-2.3239
1	F	N	0.6850
2	E	N	-2.1671
3	F	N	-1.9363
4	E	N	-3.8644
5	F	N	-2.4192
6	E	N	-3.6753
7	G	N	-2.1099
8	G	N	-2.3405
9	G	N	-2.1682
10	K	N	-2.2621
11	G	N	-2.3759
12	H	N	-2.2584
1	F	O	0.6623
2	E	O	-1.9964
3	F	O	-1.7263
4	E	O	-3.7457
5	F	O	-2.3147
6	E	O	-3.5885
7	G	O	-2.2181
8	G	O	-2.3882
9	G	O	-2.2484
10	K	O	-2.9109
11	G	O	-2.5253
12	H	O	-2.2442
1	F	P	1.1962
2	E	P	-1.6161
3	F	P	-1.5283
4	E	P	-3.6380
5	F	P	-2.6285
6	E	P	-3.5966
7	G	P	-2.0858
8	G	P	-2.2875
9	G	P	-2.1451
10	K	P	-2.3732
11	G	P	-2.5109
12	H	P	-2.3792
1	F	Q	1.4081
2	E	Q	-1.5612
3	F	Q	-1.4137
4	E	Q	-3.6825
5	F	Q	-2.3125
6	E	Q	-3.5255
7	G	Q	-2.0392
8	G	Q	-2.0724
9	G	Q	-1.7740
10	K	Q	0.0000
11	G	Q	-2.0659
12	H	Q	-2.1453
1	F	R	1.4535
2	E	R	-1.3181
3	F	R	0.0000
4	E	R	-3.5979
5	F	R	0.0000
6	E	R	-3.4320
7	G	R	-1.9755
8	G	R	-1.8749
9	G	R	-1.7378
10	K	R	0.0000
11	G	R	-1.9468
12	H	R	-1.9771
1	F	S	1.6474
2	E	S	-1.3665
3	F	S	0.0000
4	E	S	-3.7146
5	F	S	0.0000
6	E	S	-3.4723
7	G	S	0.0000
8	G	S	-2.0452
9	G	S	-2.1838
10	K	S	-2.5928
11	G	S	-2.4053
12	H	S	-2.5629
1	F	T	0.6958
2	E	T	-2.3296
3	F	T	0.0000
4	E	T	-4.9815
5	F	T	0.0000
6	E	T	-3.4894
7	G	T	-2.0436
8	G	T	-1.8901
9	G	T	0.0000
10	K	T	-1.9880
11	G	T	-1.9009
12	H	T	-1.4885