Project name: obj1 [mutate: LG18C, SM21C, VF118C, SW120C]

Chain sequence(s)	C: EVQLVESGGGLVQPGGSLRLSCAASDFTFRSYEMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARLRDGFNKGFDYWGQGTLVTVSS
Selected Chain(s)	C
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No
Mutated residues	SM21C,LG18C,SW120C,VF118C
Energy difference between WT (input) and mutated protein (by FoldX)	10.3648 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with C chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       FoldX:    Building mutant model                                                       (00:00:24)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:45)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:01:09)
[INFO]       Main:     Simulation completed successfully.                                          (00:01:10)

• Minimal score value: -3.3333
• Maximal score value: 1.6805
• Average score: -0.6247
• Total score value: -74.9625

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	C	-2.0063
2	V	C	-0.9165
3	Q	C	-1.2622
4	L	C	0.0000
5	V	C	0.5584
6	E	C	0.2600
7	S	C	-0.3375
8	G	C	-0.9112
9	G	C	-0.2575
10	G	C	0.7201
11	L	C	1.3689
12	V	C	0.1023
13	Q	C	-0.9968
14	P	C	-1.0927
15	G	C	-1.3728
16	G	C	-0.9802
17	S	C	-1.3426
18	G	C	-1.2962	mutated: LG18C
19	R	C	-2.2856
20	L	C	0.0000
21	M	C	-0.3212	mutated: SM21C
22	C	C	0.0000
23	A	C	-0.1809
24	A	C	0.0000
25	S	C	-0.2151
26	D	C	0.0000
27	F	C	1.5458
28	T	C	0.2527
29	F	C	0.0000
30	R	C	-2.0319
31	S	C	-0.8861
32	Y	C	-1.2160
33	E	C	-1.1454
34	M	C	0.0000
35	S	C	0.0000
36	W	C	0.0000
37	V	C	0.0000
38	R	C	0.0000
39	Q	C	-0.2986
40	A	C	-0.9863
41	P	C	-1.2936
42	G	C	-1.4347
43	K	C	-2.1362
44	G	C	-1.0612
45	L	C	0.3908
46	E	C	-0.3994
47	W	C	0.3362
48	V	C	0.0000
49	S	C	0.0000
50	A	C	0.0000
51	I	C	0.0000
52	S	C	-0.5832
53	G	C	-1.2444
54	S	C	-1.2283
55	G	C	-1.0815
56	G	C	-0.7345
57	S	C	-0.3026
58	T	C	0.1984
59	Y	C	0.6071
60	Y	C	-0.3573
61	A	C	-1.1408
62	D	C	-2.3454
63	S	C	-1.7169
64	V	C	0.0000
65	K	C	-2.3855
66	G	C	-1.6178
67	R	C	0.0000
68	F	C	0.0000
69	T	C	-0.6947
70	I	C	0.0000
71	S	C	-0.5775
72	R	C	-1.3668
73	D	C	-2.0011
74	N	C	-2.1883
75	S	C	-1.7897
76	K	C	-2.3192
77	N	C	-1.6541
78	T	C	0.0000
79	L	C	0.0000
80	Y	C	0.0000
81	L	C	0.0000
82	Q	C	-1.3076
83	M	C	0.0000
84	N	C	-1.3902
85	S	C	-1.2501
86	L	C	0.0000
87	R	C	-2.1723
88	A	C	-1.3931
89	E	C	-2.1478
90	D	C	0.0000
91	T	C	-0.2786
92	A	C	0.0000
93	I	C	1.0382
94	Y	C	0.0000
95	Y	C	0.6367
96	C	C	0.0000
97	A	C	0.0000
98	R	C	0.0000
99	L	C	0.0000
100	R	C	-3.1907
101	D	C	-3.3333
102	G	C	-2.0866
103	F	C	-1.2310
104	N	C	-2.4286
105	K	C	-3.1848
106	G	C	-1.9076
107	F	C	-0.9859
108	D	C	-1.0994
109	Y	C	-0.2104
110	W	C	0.5480
111	G	C	-0.0929
112	Q	C	-0.8885
113	G	C	0.0806
114	T	C	0.5453
115	L	C	1.6805
116	V	C	0.0000
117	T	C	0.4514
118	F	C	0.0000	mutated: VF118C
119	S	C	-0.0701
120	W	C	0.3599	mutated: SW120C