| Chain sequence(s) |
A: SFEGQMAEYPTISIDRFDRENLRARAYFLSHCHKDHMKGLRAPTLKRRLECLKVYLYCSPVTKELLLTSPKYRFWKKRIISIEIETPTQISLVDEASGEKEEIVVTLLPAGHCPGSVMFLFQGNNGTVLYTGDFRLAQGEAARMELLHSGGRVKDIQSVYLDTTFCDPRFYQIPSREECLSGVLELVRSWITRSPYHVVWLNCKAAYGYEYLFTNLSEELGVQVHVNKLDMFRNMPEILHHLTTDRNTQIHACRHPKAEEYFQWSKLPCGITSRNRIPLHIISIKPSTMWFGERSTNVIVRTGESSYRACFSFHSSYSEIKDFLSYLCPVNAYPNVIPVGTTMDKVVEILKPLCRS
input PDB |
| Selected Chain(s) | A |
| Distance of aggregation | 10 Å |
| FoldX usage | Yes |
| Dynamic mode | No |
| Automated mutations | No |
| Mutated residues | AP28A |
| Energy difference between WT (input) and mutated protein (by FoldX) | 4.56022 kcal/mol
CAUTION: Your mutation/s can destabilize the protein structure |
| Downloads | Download all the data |
| Simulation log |
[INFO] Logger: Verbosity set to: 2 - [INFO] (00:00:00)
[WARNING] runJob: Working directory already exists (possibly overwriting previous results -ow
to prevent this behavior) (00:00:00)
[INFO] runJob: Starting aggrescan3d job on: input.pdb with A chain(s) selected (00:00:00)
[INFO] runJob: Creating pdb object from: input.pdb (00:00:00)
[INFO] FoldX: Starting FoldX energy minimalization (00:00:01)
[INFO] FoldX: Building mutant model (00:02:15)
[INFO] FoldX: Starting FoldX energy minimalization (00:02:24)
[INFO] Analysis: Starting Aggrescan3D on folded.pdb (00:04:17)
[INFO] Main: Simulation completed successfully. (00:04:19)
|
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 3 | S | A | -0.5362 | |
| 4 | F | A | 0.0000 | |
| 5 | E | A | -2.3063 | |
| 6 | G | A | 0.0000 | |
| 7 | Q | A | -1.3882 | |
| 8 | M | A | 0.0000 | |
| 9 | A | A | -0.3719 | |
| 10 | E | A | -0.9269 | |
| 11 | Y | A | 0.0000 | |
| 12 | P | A | -0.6090 | |
| 13 | T | A | -0.8204 | |
| 14 | I | A | 0.0000 | |
| 15 | S | A | 0.0000 | |
| 16 | I | A | 0.0000 | |
| 17 | D | A | 0.0000 | |
| 18 | R | A | -1.9107 | |
| 19 | F | A | 0.0000 | |
| 20 | D | A | -2.3253 | |
| 21 | R | A | -3.1147 | |
| 22 | E | A | -3.2883 | |
| 23 | N | A | 0.0000 | |
| 24 | L | A | -1.7984 | |
| 25 | R | A | -2.7992 | |
| 26 | A | A | 0.0000 | |
| 27 | R | A | -0.7871 | |
| 28 | P | A | 0.0000 | mutated: AP28A |
| 29 | Y | A | 0.0000 | |
| 30 | F | A | 0.0000 | |
| 31 | L | A | 0.0000 | |
| 32 | S | A | 0.0000 | |
| 33 | H | A | 0.0000 | |
| 34 | C | A | -0.5837 | |
| 35 | H | A | 0.0000 | |
| 36 | K | A | -2.8084 | |
| 37 | D | A | -2.9572 | |
| 38 | H | A | -2.0429 | |
| 39 | M | A | -2.0257 | |
| 40 | K | A | -2.5753 | |
| 41 | G | A | -1.8219 | |
| 42 | L | A | 0.0000 | |
| 43 | R | A | -2.7091 | |
| 44 | A | A | -1.6296 | |
| 45 | P | A | -1.5883 | |
| 46 | T | A | -1.3572 | |
| 47 | L | A | 0.0000 | |
| 48 | K | A | -2.5337 | |
| 49 | R | A | -2.8984 | |
| 50 | R | A | -1.8705 | |
| 51 | L | A | 0.0000 | |
| 52 | E | A | -2.3097 | |
| 53 | C | A | -0.5130 | |
| 55 | L | A | 0.1853 | |
| 56 | K | A | -1.1757 | |
| 57 | V | A | 0.0000 | |
| 58 | Y | A | 0.0361 | |
| 59 | L | A | 0.0000 | |
| 60 | Y | A | 0.0000 | |
| 61 | C | A | 0.0000 | |
| 62 | S | A | 0.0000 | |
| 63 | P | A | -0.3959 | |
| 64 | V | A | -0.2785 | |
| 65 | T | A | 0.0000 | |
| 66 | K | A | -0.6274 | |
| 67 | E | A | -1.1865 | |
| 68 | L | A | 0.0000 | |
| 69 | L | A | 0.0000 | |
| 70 | L | A | -1.4350 | |
| 71 | T | A | -1.1215 | |
| 72 | S | A | -1.1813 | |
| 73 | P | A | -1.5577 | |
| 74 | K | A | -2.6085 | |
| 75 | Y | A | 0.0000 | |
| 76 | R | A | -3.1223 | |
| 77 | F | A | -2.0167 | |
| 78 | W | A | 0.0000 | |
| 79 | K | A | -2.9884 | |
| 80 | K | A | -2.5498 | |
| 81 | R | A | -1.5236 | |
| 82 | I | A | 0.0000 | |
| 83 | I | A | 0.2771 | |
| 84 | S | A | -0.1262 | |
| 85 | I | A | 0.0000 | |
| 86 | E | A | -1.9408 | |
| 87 | I | A | -1.3332 | |
| 88 | E | A | -2.2382 | |
| 89 | T | A | -1.4143 | |
| 90 | P | A | -0.9929 | |
| 91 | T | A | -0.9569 | |
| 92 | Q | A | -1.4233 | |
| 93 | I | A | 0.0000 | |
| 94 | S | A | -1.4293 | |
| 95 | L | A | 0.0000 | |
| 96 | V | A | -1.4366 | |
| 97 | D | A | -2.3402 | |
| 98 | E | A | -2.5241 | |
| 99 | A | A | -1.2763 | |
| 100 | S | A | -1.6280 | |
| 101 | G | A | -2.1459 | |
| 102 | E | A | -3.1349 | |
| 103 | K | A | -3.1753 | |
| 104 | E | A | -2.5727 | |
| 105 | E | A | -2.3513 | |
| 106 | I | A | 0.0000 | |
| 107 | V | A | -0.4992 | |
| 108 | V | A | 0.0000 | |
| 109 | T | A | 0.0000 | |
| 110 | L | A | 0.0000 | |
| 111 | L | A | 0.0000 | |
| 112 | P | A | -0.3951 | |
| 113 | A | A | 0.0000 | |
| 114 | G | A | -0.2949 | |
| 115 | H | A | 0.0000 | |
| 116 | C | A | 0.1374 | |
| 117 | P | A | 0.1520 | |
| 118 | G | A | -0.0083 | |
| 119 | S | A | 0.0000 | |
| 120 | V | A | 0.0000 | |
| 121 | M | A | 0.0000 | |
| 122 | F | A | 0.0000 | |
| 123 | L | A | 0.0000 | |
| 124 | F | A | 0.0000 | |
| 125 | Q | A | -1.1373 | |
| 126 | G | A | -1.8255 | |
| 127 | N | A | -2.3194 | |
| 128 | N | A | -1.9460 | |
| 129 | G | A | -1.6179 | |
| 130 | T | A | -1.2387 | |
| 131 | V | A | 0.0000 | |
| 132 | L | A | 0.0000 | |
| 133 | Y | A | 0.0000 | |
| 134 | T | A | 0.0000 | |
| 135 | G | A | 0.0000 | |
| 136 | D | A | 0.0000 | |
| 137 | F | A | 0.0000 | |
| 138 | R | A | 0.0000 | |
| 139 | L | A | 0.0000 | |
| 140 | A | A | -1.0014 | |
| 141 | Q | A | -1.8181 | |
| 142 | G | A | -1.7881 | |
| 143 | E | A | -2.0042 | |
| 144 | A | A | 0.0000 | |
| 145 | A | A | -1.9181 | |
| 146 | R | A | -2.6820 | |
| 147 | M | A | 0.0000 | |
| 148 | E | A | -2.8287 | |
| 149 | L | A | -1.3681 | |
| 150 | L | A | 0.0000 | |
| 151 | H | A | -2.0715 | |
| 152 | S | A | -1.7501 | |
| 153 | G | A | -1.6172 | |
| 154 | G | A | -1.9164 | |
| 155 | R | A | -2.7123 | |
| 156 | V | A | -1.5423 | |
| 157 | K | A | -1.8754 | |
| 158 | D | A | -2.0844 | |
| 159 | I | A | 0.0000 | |
| 160 | Q | A | -1.0807 | |
| 161 | S | A | 0.0000 | |
| 162 | V | A | 0.0000 | |
| 163 | Y | A | 0.0000 | |
| 164 | L | A | 0.0000 | |
| 165 | D | A | 0.0000 | |
| 166 | T | A | 0.0000 | |
| 167 | T | A | 0.4673 | |
| 168 | F | A | -0.0094 | |
| 169 | C | A | 0.0000 | |
| 170 | D | A | -0.8851 | |
| 171 | P | A | -1.3074 | |
| 172 | R | A | -2.0038 | |
| 173 | F | A | -0.7300 | |
| 174 | Y | A | -1.1950 | |
| 175 | Q | A | -0.8070 | |
| 176 | I | A | 0.0000 | |
| 177 | P | A | 0.0000 | |
| 178 | S | A | -1.5170 | |
| 179 | R | A | -1.5781 | |
| 180 | E | A | -2.7519 | |
| 181 | E | A | -2.8610 | |
| 182 | C | A | 0.0000 | |
| 183 | L | A | -1.2952 | |
| 184 | S | A | -1.5483 | |
| 185 | G | A | -1.1860 | |
| 186 | V | A | 0.0000 | |
| 187 | L | A | -1.1132 | |
| 188 | E | A | -1.3940 | |
| 189 | L | A | -0.5547 | |
| 190 | V | A | 0.0000 | |
| 191 | R | A | -1.5167 | |
| 192 | S | A | -1.0959 | |
| 193 | W | A | -0.9783 | |
| 194 | I | A | 0.0000 | |
| 195 | T | A | -1.1684 | |
| 196 | R | A | -2.0920 | |
| 197 | S | A | -1.0959 | |
| 198 | P | A | -0.6852 | |
| 199 | Y | A | -0.5276 | |
| 200 | H | A | -0.8374 | |
| 201 | V | A | 0.0000 | |
| 202 | V | A | 0.0000 | |
| 203 | W | A | 0.0000 | |
| 204 | L | A | 0.0000 | |
| 205 | N | A | -1.2223 | |
| 206 | C | A | 0.0000 | |
| 207 | K | A | -1.8636 | |
| 208 | A | A | -0.8270 | |
| 209 | A | A | -0.5898 | |
| 210 | Y | A | 0.0744 | |
| 211 | G | A | 0.0000 | |
| 212 | Y | A | 0.0000 | |
| 213 | E | A | 0.0000 | |
| 214 | Y | A | 0.1999 | |
| 215 | L | A | 0.0000 | |
| 216 | F | A | 0.0000 | |
| 217 | T | A | -1.2720 | |
| 218 | N | A | -1.3673 | |
| 219 | L | A | 0.0000 | |
| 220 | S | A | 0.0000 | |
| 221 | E | A | -3.1039 | |
| 222 | E | A | -2.8332 | |
| 223 | L | A | -1.6028 | |
| 224 | G | A | -1.5440 | |
| 225 | V | A | -1.0470 | |
| 226 | Q | A | -1.7125 | |
| 227 | V | A | 0.0000 | |
| 228 | H | A | 0.0000 | |
| 229 | V | A | 0.0000 | |
| 230 | N | A | -2.6097 | |
| 231 | K | A | -2.3727 | |
| 232 | L | A | -1.5139 | |
| 233 | D | A | -1.9303 | |
| 234 | M | A | -1.2043 | |
| 235 | F | A | 0.0000 | |
| 236 | R | A | -2.5990 | |
| 237 | N | A | -2.4134 | |
| 238 | M | A | 0.0000 | |
| 239 | P | A | -1.9101 | |
| 240 | E | A | -2.1735 | |
| 241 | I | A | -1.2590 | |
| 242 | L | A | -1.4701 | |
| 243 | H | A | -1.8831 | |
| 244 | H | A | -1.5539 | |
| 245 | L | A | 0.0000 | |
| 246 | T | A | 0.0000 | |
| 247 | T | A | -2.2240 | |
| 248 | D | A | -3.1131 | |
| 249 | R | A | -3.0237 | |
| 250 | N | A | -2.6746 | |
| 251 | T | A | -1.5628 | |
| 252 | Q | A | -0.7534 | |
| 253 | I | A | 0.0000 | |
| 254 | H | A | 0.0000 | |
| 255 | A | A | -0.5657 | |
| 256 | C | A | 0.0000 | |
| 257 | R | A | -1.6585 | |
| 258 | H | A | -1.4181 | |
| 259 | P | A | -1.3551 | |
| 260 | K | A | -1.9778 | |
| 261 | A | A | 0.0000 | |
| 262 | E | A | -2.6643 | |
| 263 | E | A | -1.9720 | |
| 264 | Y | A | -1.0688 | |
| 265 | F | A | -0.9277 | |
| 266 | Q | A | -1.2369 | |
| 267 | W | A | 0.1867 | |
| 268 | S | A | -0.4259 | |
| 269 | K | A | -0.5590 | |
| 270 | L | A | 0.0000 | |
| 271 | P | A | 0.0000 | |
| 272 | C | A | -0.6281 | |
| 273 | G | A | -0.3178 | |
| 274 | I | A | -0.4131 | |
| 275 | T | A | -0.2929 | |
| 276 | S | A | -1.1706 | |
| 277 | R | A | -1.8509 | |
| 278 | N | A | -2.2557 | |
| 279 | R | A | -2.3802 | |
| 280 | I | A | -0.8268 | |
| 281 | P | A | -0.8575 | |
| 282 | L | A | 0.0000 | |
| 283 | H | A | -1.0141 | |
| 284 | I | A | 0.0000 | |
| 285 | I | A | 0.0000 | |
| 286 | S | A | 0.0000 | |
| 287 | I | A | 0.0000 | |
| 288 | K | A | -1.1095 | |
| 289 | P | A | 0.0000 | |
| 290 | S | A | -0.5307 | |
| 291 | T | A | 0.0349 | |
| 292 | M | A | 0.0000 | |
| 293 | W | A | 0.2526 | |
| 294 | F | A | -0.5830 | |
| 295 | G | A | -1.9005 | |
| 296 | E | A | -2.9655 | |
| 297 | R | A | -2.7308 | |
| 298 | S | A | -1.5411 | |
| 301 | T | A | -0.4834 | |
| 302 | N | A | -1.1198 | |
| 303 | V | A | -0.4284 | |
| 304 | I | A | -0.2064 | |
| 305 | V | A | -0.3685 | |
| 306 | R | A | -1.8479 | |
| 307 | T | A | -1.3213 | |
| 308 | G | A | -1.5777 | |
| 309 | E | A | -2.2698 | |
| 310 | S | A | -1.4888 | |
| 311 | S | A | -1.1642 | |
| 312 | Y | A | -1.0752 | |
| 313 | R | A | -0.8226 | |
| 314 | A | A | 0.0000 | |
| 315 | C | A | 0.0000 | |
| 316 | F | A | 0.0000 | |
| 317 | S | A | 0.0000 | |
| 318 | F | A | 0.1107 | |
| 319 | H | A | -0.0363 | |
| 320 | S | A | 0.0000 | |
| 321 | S | A | 0.0000 | |
| 322 | Y | A | 0.0000 | |
| 323 | S | A | -0.9173 | |
| 324 | E | A | 0.0000 | |
| 325 | I | A | 0.0000 | |
| 326 | K | A | -0.9960 | |
| 327 | D | A | -1.4331 | |
| 328 | F | A | 0.0000 | |
| 329 | L | A | 0.0000 | |
| 330 | S | A | -0.2290 | |
| 331 | Y | A | 0.2026 | |
| 332 | L | A | 0.0000 | |
| 333 | C | A | -0.1564 | |
| 334 | P | A | 0.0000 | |
| 335 | V | A | -0.2310 | |
| 336 | N | A | -0.7700 | |
| 337 | A | A | -0.2372 | |
| 338 | Y | A | 0.2677 | |
| 339 | P | A | 0.0000 | |
| 340 | N | A | 0.0000 | |
| 341 | V | A | 0.7815 | |
| 342 | I | A | 0.9098 | |
| 343 | P | A | 1.0946 | |
| 344 | V | A | 1.7042 | |
| 345 | G | A | 0.4489 | |
| 346 | T | A | -0.0293 | |
| 347 | T | A | -0.4425 | |
| 348 | M | A | -0.6327 | |
| 349 | D | A | -2.3209 | |
| 350 | K | A | -2.1360 | |
| 351 | V | A | 0.0000 | |
| 352 | V | A | -1.6435 | |
| 353 | E | A | -2.8563 | |
| 354 | I | A | -1.6235 | |
| 355 | L | A | 0.0000 | |
| 356 | K | A | -2.1948 | |
| 357 | P | A | -1.4285 | |
| 358 | L | A | -0.8337 | |
| 359 | C | A | -0.6942 | |
| 360 | R | A | -0.9055 | |
| 361 | S | A | -0.6171 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine