Project name: obj1 [mutate: VE2C, LG4C, QC13C, GA10C, RN30C, TC28C, FE27C, WG36C, SF52C, VT64C, NV74C, SP85C, IM93C, KH105C, GL113C, VY118C]

Chain sequence(s)	C: EVQLVESGGGLVQPGGSLRLSCAASDFTFRSYEMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARLRDGFNKGFDYWGQGTLVTVSS
Selected Chain(s)	C
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No
Mutated residues	IM93C,VY118C,WG36C,FE27C,GL113C,TC28C,SP85C,NV74C,SF52C,KH105C,QC13C,VE2C,GA10C,VT64C,RN30C,LG4C
Energy difference between WT (input) and mutated protein (by FoldX)	45.1484 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with C chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       FoldX:    Building mutant model                                                       (00:00:25)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:02:28)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:02:52)
[INFO]       Main:     Simulation completed successfully.                                          (00:02:52)

• Minimal score value: -3.1482
• Maximal score value: 1.9937
• Average score: -0.4901
• Total score value: -58.8132

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	C	-2.1949
2	E	C	-1.8240	mutated: VE2C
3	Q	C	-1.3227
4	G	C	0.0903	mutated: LG4C
5	V	C	1.1558
6	E	C	0.5605
7	S	C	-0.2003
8	G	C	-0.7290
9	G	C	0.2133
10	A	C	1.1795	mutated: GA10C
11	L	C	1.9937
12	V	C	0.9669
13	C	C	0.5821	mutated: QC13C
14	P	C	-0.5675
15	G	C	-0.9217
16	G	C	-0.3383
17	S	C	-0.9071
18	L	C	-0.8743
19	R	C	-2.1167
20	L	C	0.0000
21	S	C	-0.4562
22	C	C	0.0000
23	A	C	-0.0174
24	A	C	0.0000
25	S	C	-1.0775
26	D	C	0.0000
27	E	C	-1.9772	mutated: FE27C
28	C	C	-0.8636	mutated: TC28C
29	F	C	0.0000
30	N	C	-1.1848	mutated: RN30C
31	S	C	-0.5136
32	Y	C	-0.5998
33	E	C	-0.3675
34	M	C	0.0000
35	S	C	0.0000
36	G	C	0.1305	mutated: WG36C
37	V	C	0.0000
38	R	C	0.0000
39	Q	C	-0.3723
40	A	C	-1.0679
41	P	C	-1.3344
42	G	C	-1.4376
43	K	C	-2.1504
44	G	C	-1.1067
45	L	C	0.2373
46	E	C	-0.4627
47	W	C	0.2927
48	V	C	0.0000
49	S	C	0.0000
50	A	C	0.0000
51	I	C	0.0000
52	F	C	0.9897	mutated: SF52C
53	G	C	0.0027
54	S	C	-0.3353
55	G	C	-0.2895
56	G	C	-0.3427
57	S	C	0.2116
58	T	C	0.5741
59	Y	C	0.8018
60	Y	C	-0.3498
61	A	C	-1.1367
62	D	C	-2.3422
63	S	C	-1.7166
64	T	C	0.0000	mutated: VT64C
65	K	C	-2.3815
66	G	C	-1.6020
67	R	C	0.0000
68	F	C	0.0000
69	T	C	-0.6519
70	I	C	0.0000
71	S	C	-0.2609
72	R	C	-0.7323
73	D	C	-1.2298
74	V	C	-0.5698	mutated: NV74C
75	S	C	-1.0419
76	K	C	-1.8766
77	N	C	-1.5102
78	T	C	0.0000
79	L	C	0.0000
80	Y	C	-0.6813
81	L	C	0.0000
82	Q	C	-1.2081
83	M	C	0.0000
84	N	C	-1.2118
85	P	C	-0.9700	mutated: SP85C
86	L	C	0.0000
87	R	C	-2.1741
88	A	C	-1.6224
89	E	C	-2.3541
90	D	C	0.0000
91	T	C	-0.4701
92	A	C	0.0000
93	M	C	0.8710	mutated: IM93C
94	Y	C	0.0000
95	Y	C	0.7431
96	C	C	0.0000
97	A	C	0.0000
98	R	C	0.0000
99	L	C	0.0000
100	R	C	-3.0230
101	D	C	-3.1482
102	G	C	-1.7358
103	F	C	-0.9190
104	N	C	-2.1440
105	H	C	-2.5076	mutated: KH105C
106	G	C	-1.5908
107	F	C	-0.8376
108	D	C	-1.0785
109	Y	C	-0.3073
110	W	C	0.6114
111	G	C	0.1489
112	Q	C	-0.6108
113	L	C	0.4905	mutated: GL113C
114	T	C	0.7823
115	L	C	1.7950
116	V	C	0.0000
117	T	C	0.6375
118	Y	C	0.0000	mutated: VY118C
119	S	C	-0.2138
120	S	C	-0.7113