Aggrescan3D: 78d5b6ad0a2f989

Project name: 78d5b6ad0a2f989

Status: done

Started: 2025-02-07 04:19:47

Settings

Chain sequence(s)	A: FCPGPVTLCSDLESHSTEAVLGDALVDFSLKLYHAFSAMKKVETNMAFSPFSIASLLTQVLLGAGENTKTNLESILSYPKDFTCVHQALKGFTTKGVTSVSQIFHSPDLAIRDTFVNASRTLYSSSPRVLSNNSDANLELINTWVAKNTNNKISRLLDSLPSDTRLVLLNAIYLSAKWKTTFDPKKTRMEPFHFKNSVIKVPMMNSKKYPVAHFIDQTLKAKVGQLQLSHNLSLVILVPQNLKHRLEDMEQALSPSVFKAIMEKLEMSKFQPTLLTLPRIKVTTSQDMLSIMEKLEFFDFSYDLNLCGLTEDPDLQVSAMQHQTVLELTETGVEAAAASAISVARTLLVFEVQQPFLFVLWDQQHKFPVFMGRVYDPR G: GDVAIATTKASEHGINVTGRTAEVRVTGGTIRTSGNQAQGLRVGTENVASVFLQNLIIETSGTGALGVSVHEPQGGGGTRLSMSGTTVRTRGDDSFALQLSGPASATLNDVALETAGQQAPAVVLWQGAQLNAQGLVVQVNGAGVSAIHAQDAGSFTLSGSDITARGLEVVGIYVQEGMQGTLTGTRVTTQGDTAPALQVEDAGTHVSMNGGALSTSGANSPAAWLLAGGSAQFRDTVLRTVGEASHGVDVAAHSEVELAHAQVRADGQGAHGLVVTRSSAMVRAGSLVESTGDGAAALLESGHLTVDGSVVHGHGAAGLEVDGESNVSLLNGARLSSDQPTAIRLIDPRSVLNLDIKDRAQLLGDIAPEAQQPEQARVRVALADGGTWAGRTDGVHTVRLLDRGVWTVTGDSRVAEVKLE input PDB
Selected Chain(s)	A,G
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	Yes
Downloads	Download all the data

Simulation log

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[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:11:14)
[INFO]       Auto_mut: Residue number 58 from chain G and a score of 2.043 (isoleucine) selected   
                       for automated muatation                                                     (00:11:20)
[INFO]       Auto_mut: Residue number 122 from chain A and a score of 1.812 (phenylalanine)        
                       selected for automated muatation                                            (00:11:21)
[INFO]       Auto_mut: Residue number 57 from chain G and a score of 1.468 (alanine) selected for  
                       automated muatation                                                         (00:11:21)
[INFO]       Auto_mut: Residue number 129 from chain A and a score of 1.401 (leucine) selected for 
                       automated muatation                                                         (00:11:21)
[INFO]       Auto_mut: Residue number 56 from chain G and a score of 1.082 (valine) selected for   
                       automated muatation                                                         (00:11:21)
[INFO]       Auto_mut: Residue number 128 from chain A and a score of 1.024 (threonine) selected   
                       for automated muatation                                                     (00:11:21)
[INFO]       Auto_mut: Mutating residue number 58 from chain G (isoleucine) into glutamic acid     (00:11:21)
[INFO]       Auto_mut: Mutating residue number 58 from chain G (isoleucine) into aspartic acid     (00:11:21)
[INFO]       Auto_mut: Mutating residue number 122 from chain A (phenylalanine) into glutamic acid 
                       Mutating residue number 122 from chain A (phenylalanine) into glutamic acid (00:11:21)
[INFO]       Auto_mut: Mutating residue number 58 from chain G (isoleucine) into lysine            (00:16:06)
[INFO]       Auto_mut: Mutating residue number 58 from chain G (isoleucine) into arginine          (00:16:08)
[INFO]       Auto_mut: Mutating residue number 122 from chain A (phenylalanine) into lysine        (00:16:11)
[INFO]       Auto_mut: Mutating residue number 122 from chain A (phenylalanine) into aspartic acid 
                       Mutating residue number 122 from chain A (phenylalanine) into aspartic acid (00:20:57)
[INFO]       Auto_mut: Mutating residue number 57 from chain G (alanine) into glutamic acid        (00:20:58)
[INFO]       Auto_mut: Mutating residue number 57 from chain G (alanine) into aspartic acid        (00:21:06)
[INFO]       Auto_mut: Mutating residue number 57 from chain G (alanine) into lysine               (00:25:47)
[INFO]       Auto_mut: Mutating residue number 122 from chain A (phenylalanine) into arginine      (00:25:50)
[INFO]       Auto_mut: Mutating residue number 57 from chain G (alanine) into arginine             (00:25:59)
[INFO]       Auto_mut: Mutating residue number 129 from chain A (leucine) into glutamic acid       (00:30:40)
[INFO]       Auto_mut: Mutating residue number 129 from chain A (leucine) into aspartic acid       (00:30:44)
[INFO]       Auto_mut: Mutating residue number 56 from chain G (valine) into glutamic acid         (00:30:52)
[INFO]       Auto_mut: Mutating residue number 129 from chain A (leucine) into arginine            (00:35:40)
[INFO]       Auto_mut: Mutating residue number 56 from chain G (valine) into lysine                (00:35:43)
[INFO]       Auto_mut: Mutating residue number 129 from chain A (leucine) into lysine              (00:35:45)
[INFO]       Auto_mut: Mutating residue number 56 from chain G (valine) into aspartic acid         (00:40:37)
[INFO]       Auto_mut: Mutating residue number 128 from chain A (threonine) into glutamic acid     (00:40:41)
[INFO]       Auto_mut: Mutating residue number 128 from chain A (threonine) into aspartic acid     (00:41:05)
[INFO]       Auto_mut: Mutating residue number 56 from chain G (valine) into arginine              (00:45:27)
[INFO]       Auto_mut: Mutating residue number 128 from chain A (threonine) into arginine          (00:45:54)
[INFO]       Auto_mut: Mutating residue number 128 from chain A (threonine) into lysine            (00:45:56)
[INFO]       Auto_mut: Effect of mutation residue number 58 from chain G (isoleucine) into         
                       glutamic acid: Energy difference: 0.0167 kcal/mol, Difference in average    
                       score from the base case: -0.0124                                           (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 58 from chain G (isoleucine) into lysine: 
                       Energy difference: -0.1414 kcal/mol, Difference in average score from the   
                       base case: -0.0137                                                          (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 58 from chain G (isoleucine) into         
                       aspartic acid: Energy difference: 0.4255 kcal/mol, Difference in average    
                       score from the base case: -0.0122                                           (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 58 from chain G (isoleucine) into         
                       arginine: Energy difference: -0.0293 kcal/mol, Difference in average score  
                       from the base case: -0.0154                                                 (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 122 from chain A (phenylalanine) into     
                       glutamic acid: Energy difference: 0.1381 kcal/mol, Difference in average    
                       score from the base case: -0.0086                                           (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 122 from chain A (phenylalanine) into     
                       lysine: Energy difference: 0.2008 kcal/mol, Difference in average score     
                       from the base case: -0.0053                                                 (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 122 from chain A (phenylalanine) into     
                       aspartic acid: Energy difference: -0.0705 kcal/mol, Difference in average   
                       score from the base case: -0.0088                                           (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 122 from chain A (phenylalanine) into     
                       arginine: Energy difference: -0.0486 kcal/mol, Difference in average score  
                       from the base case: -0.0054                                                 (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 57 from chain G (alanine) into glutamic   
                       acid: Energy difference: -1.0662 kcal/mol, Difference in average score from 
                       the base case: -0.0047                                                      (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 57 from chain G (alanine) into lysine:    
                       Energy difference: -1.4075 kcal/mol, Difference in average score from the   
                       base case: -0.0083                                                          (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 57 from chain G (alanine) into aspartic   
                       acid: Energy difference: -1.4897 kcal/mol, Difference in average score from 
                       the base case: -0.0055                                                      (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 57 from chain G (alanine) into arginine:  
                       Energy difference: -1.3506 kcal/mol, Difference in average score from the   
                       base case: -0.0089                                                          (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 129 from chain A (leucine) into glutamic  
                       acid: Energy difference: -0.3419 kcal/mol, Difference in average score from 
                       the base case: -0.0107                                                      (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 129 from chain A (leucine) into lysine:   
                       Energy difference: -0.0934 kcal/mol, Difference in average score from the   
                       base case: -0.0085                                                          (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 129 from chain A (leucine) into aspartic  
                       acid: Energy difference: 0.1544 kcal/mol, Difference in average score from  
                       the base case: -0.0106                                                      (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 129 from chain A (leucine) into arginine: 
                       Energy difference: 0.1709 kcal/mol, Difference in average score from the    
                       base case: -0.0065                                                          (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 56 from chain G (valine) into glutamic    
                       acid: Energy difference: -0.4493 kcal/mol, Difference in average score from 
                       the base case: -0.0124                                                      (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 56 from chain G (valine) into lysine:     
                       Energy difference: -0.4429 kcal/mol, Difference in average score from the   
                       base case: -0.0139                                                          (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 56 from chain G (valine) into aspartic    
                       acid: Energy difference: -0.0406 kcal/mol, Difference in average score from 
                       the base case: -0.0143                                                      (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 56 from chain G (valine) into arginine:   
                       Energy difference: -0.8952 kcal/mol, Difference in average score from the   
                       base case: -0.0145                                                          (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 128 from chain A (threonine) into         
                       glutamic acid: Energy difference: -0.2202 kcal/mol, Difference in average   
                       score from the base case: -0.0052                                           (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 128 from chain A (threonine) into lysine: 
                       Energy difference: -0.0516 kcal/mol, Difference in average score from the   
                       base case: -0.0034                                                          (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 128 from chain A (threonine) into         
                       aspartic acid: Energy difference: -0.0991 kcal/mol, Difference in average   
                       score from the base case: -0.0032                                           (00:51:00)
[INFO]       Auto_mut: Effect of mutation residue number 128 from chain A (threonine) into         
                       arginine: Energy difference: -0.2197 kcal/mol, Difference in average score  
                       from the base case: -0.0032                                                 (00:51:00)
[INFO]       Main:     Simulation completed successfully.                                          (00:51:19)

Note Your prediction results will be deleted after 360 days.

Drag cursor over the plot to display residue labels.

Show buried residues

Minimal score value: -3.7683
Maximal score value: 2.043
Average score: -0.7815
Total score value: -622.0948

Download the table (csv)

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
residue index	residue name	chain	Aggrescan3D score	mutation
122	F	A	1.8124
123	C	A	0.7281
124	P	A	0.0431
125	G	A	-0.1015
126	P	A	0.0207
127	V	A	1.0164
128	T	A	1.0239
129	L	A	1.4015
130	C	A	0.3902
131	S	A	-0.4540
132	D	A	-1.6052
133	L	A	-0.3412
134	E	A	-1.7584
135	S	A	-1.6382
136	H	A	-1.7530
137	S	A	-1.1908
138	T	A	-0.7492
139	E	A	-1.3214
140	A	A	-0.5318
141	V	A	0.1755
142	L	A	0.0000
143	G	A	0.0000
144	D	A	-1.5014
145	A	A	0.0000
146	L	A	0.0000
147	V	A	0.0000
148	D	A	-1.5332
149	F	A	0.0000
150	S	A	0.0000
151	L	A	0.0000
152	K	A	-1.2796
153	L	A	0.0000
154	Y	A	0.0000
155	H	A	-1.0856
156	A	A	0.0000
157	F	A	0.0000
158	S	A	-0.6215
159	A	A	-0.3654
160	M	A	-0.4385
161	K	A	-0.9817
162	K	A	-1.4360
163	V	A	0.0323
164	E	A	-1.6089
165	T	A	-1.2977
166	N	A	-1.3382
167	M	A	0.0000
168	A	A	0.0000
169	F	A	0.0000
170	S	A	0.0000
171	P	A	0.0000
172	F	A	0.0000
173	S	A	0.0000
174	I	A	0.0000
175	A	A	0.0000
176	S	A	-0.1310
177	L	A	0.0000
178	L	A	0.0000
179	T	A	0.0000
180	Q	A	0.0000
181	V	A	0.0000
182	L	A	-0.2700
183	L	A	0.1922
184	G	A	0.0000
185	A	A	0.0000
186	G	A	0.0000
187	E	A	-1.7839
188	N	A	-2.5103
189	T	A	0.0000
190	K	A	-1.9550
191	T	A	-1.7988
192	N	A	-1.6024
193	L	A	0.0000
194	E	A	-1.4476
195	S	A	-0.9255
196	I	A	0.0000
197	L	A	0.0000
198	S	A	-0.8047
199	Y	A	-0.8411
200	P	A	-1.2574
201	K	A	-2.6435
202	D	A	-2.5836
203	F	A	-1.3724
204	T	A	-0.6275
205	C	A	0.0000
206	V	A	0.0000
207	H	A	-0.1658
208	Q	A	-0.7557
209	A	A	0.0000
210	L	A	0.0000
211	K	A	-1.0730
212	G	A	-1.1965
213	F	A	0.0000
214	T	A	-0.8687
215	T	A	-1.5578
216	K	A	-2.1304
217	G	A	0.0000
218	V	A	0.0000
219	T	A	-0.6658
220	S	A	-0.3687
221	V	A	-0.0183
222	S	A	0.0141
223	Q	A	0.0000
224	I	A	0.0000
225	F	A	0.0000
226	H	A	0.0000
227	S	A	-1.4660
228	P	A	-1.2809
229	D	A	-1.9704
230	L	A	-0.9757
231	A	A	-0.7609
232	I	A	-0.5319
233	R	A	-1.5258
234	D	A	-2.0817
235	T	A	-1.4220
236	F	A	0.0000
237	V	A	-1.4947
238	N	A	-2.0679
239	A	A	-0.9514
240	S	A	0.0000
241	R	A	-2.2016
242	T	A	-1.2428
243	L	A	0.0000
244	Y	A	-0.8047
245	S	A	-1.0871
246	S	A	-1.0999
247	S	A	-0.8971
248	P	A	0.0000
249	R	A	-0.4178
250	V	A	0.2184
251	L	A	0.0000
252	S	A	-1.3785
253	N	A	-2.3464
254	N	A	-2.5979
255	S	A	-2.3699
256	D	A	-2.6181
257	A	A	-1.8993
258	N	A	0.0000
259	L	A	-1.7574
260	E	A	-2.4963
261	L	A	-0.8247
262	I	A	0.0000
263	N	A	-1.7734
264	T	A	-1.4659
265	W	A	-0.9266
266	V	A	0.0000
267	A	A	-2.4364
268	K	A	-2.9432
269	N	A	-2.1030
270	T	A	0.0000
271	N	A	-2.8750
272	N	A	-3.3229
273	K	A	-2.5381
274	I	A	0.0000
275	S	A	-2.2209
276	R	A	-2.8667
277	L	A	0.0000
278	L	A	0.0000
279	D	A	-2.4748
280	S	A	-1.1072
281	L	A	-1.2473
282	P	A	-1.2459
283	S	A	-1.6345
284	D	A	-2.2984
285	T	A	-1.6414
286	R	A	-2.0690
287	L	A	0.0000
288	V	A	0.0000
289	L	A	0.0000
290	L	A	0.0000
291	N	A	0.0000
292	A	A	0.0000
293	I	A	0.0000
294	Y	A	-0.4405
295	L	A	0.0000
296	S	A	-0.9936
297	A	A	0.0000
298	K	A	-1.6870
299	W	A	0.0000
300	K	A	-1.8192
301	T	A	-1.0911
302	T	A	-0.9432
303	F	A	0.0000
304	D	A	-1.6622
305	P	A	-1.9764
306	K	A	-2.8294
307	K	A	-2.6473
308	T	A	-2.1990
309	R	A	-2.4739
310	M	A	-1.0322
311	E	A	-0.9715
312	P	A	-0.4158
313	F	A	0.0000
314	H	A	-0.3847
315	F	A	-0.8132
316	K	A	-1.5962
317	N	A	-1.8295
318	S	A	-0.5212
319	V	A	0.2711
320	I	A	-0.3381
321	K	A	-1.6293
322	V	A	0.0000
323	P	A	-1.3813
324	M	A	0.0000
325	M	A	0.0000
326	N	A	-1.5862
327	S	A	-1.0649
328	K	A	-1.3097
329	K	A	-1.2126
330	Y	A	0.0000
331	P	A	-0.7988
332	V	A	0.0000
333	A	A	0.0000
334	H	A	-0.1199
335	F	A	0.1896
336	I	A	-0.0740
337	D	A	0.0000
338	Q	A	-1.7083
339	T	A	-0.9721
340	L	A	0.0000
341	K	A	-2.1381
342	A	A	0.0000
343	K	A	-0.6696
344	V	A	0.0000
345	G	A	0.0000
346	Q	A	0.0000
347	L	A	0.0000
348	Q	A	-1.0520
349	L	A	0.0000
350	S	A	-1.7599
351	H	A	-2.2312
352	N	A	-2.5030
353	L	A	0.0000
354	S	A	0.0000
355	L	A	0.0000
356	V	A	0.0000
357	I	A	0.0000
358	L	A	0.0000
359	V	A	0.0000
360	P	A	0.0000
361	Q	A	-1.0772
362	N	A	-0.5613
363	L	A	0.3600
364	K	A	-0.7772
365	H	A	-1.3003
366	R	A	-1.4920
367	L	A	0.0000
368	E	A	-1.6678
369	D	A	-2.2458
370	M	A	0.0000
371	E	A	0.0000
372	Q	A	-1.8110
373	A	A	-1.1126
374	L	A	0.0000
375	S	A	-0.6170
376	P	A	-0.8549
377	S	A	-0.4928
378	V	A	-0.1151
379	F	A	0.0000
380	K	A	-1.5126
381	A	A	-1.1632
382	I	A	0.0000
383	M	A	0.0000
384	E	A	-2.3736
385	K	A	-1.9720
386	L	A	0.0000
387	E	A	-1.9869
388	M	A	-0.6906
389	S	A	-1.2036
390	K	A	-1.6340
391	F	A	-0.8029
392	Q	A	-0.8751
393	P	A	-0.6817
394	T	A	0.0000
395	L	A	0.0000
396	L	A	0.0000
397	T	A	-0.7236
398	L	A	0.0000
399	P	A	0.0000
400	R	A	-1.6453
401	I	A	0.0000
402	K	A	-2.9641
403	V	A	0.0000
404	T	A	-0.7396
405	T	A	0.0000
406	S	A	-0.6561
407	Q	A	-0.6841
408	D	A	-0.9265
409	M	A	0.0000
410	L	A	-0.4833
411	S	A	-0.8292
412	I	A	0.0000
413	M	A	0.0000
414	E	A	-2.0774
415	K	A	-2.6084
416	L	A	0.0000
417	E	A	-2.4599
418	F	A	0.0000
419	F	A	0.1381
420	D	A	-0.5538
421	F	A	0.0000
422	S	A	0.2709
423	Y	A	0.5613
424	D	A	-1.6158
425	L	A	0.0000
426	N	A	-1.8502
427	L	A	0.0000
428	C	A	-0.5205
429	G	A	-0.5133
430	L	A	0.0000
431	T	A	0.0000
432	E	A	-2.2340
433	D	A	-2.1170
434	P	A	-1.9577
435	D	A	-2.6869
436	L	A	-1.8427
437	Q	A	-1.3590
438	V	A	0.0000
439	S	A	-0.7739
440	A	A	0.0000
441	M	A	0.0000
442	Q	A	-0.6757
443	H	A	0.0000
444	Q	A	0.0000
445	T	A	0.0000
446	V	A	-0.7233
447	L	A	0.0000
448	E	A	-2.7606
449	L	A	0.0000
450	T	A	-1.2397
451	E	A	-0.8811
452	T	A	-0.8682
453	G	A	-0.8945
454	V	A	-0.4916
455	E	A	-1.6043
456	A	A	-0.8191
457	A	A	-0.4497
458	A	A	-0.4413
459	A	A	-0.0939
460	S	A	-0.1125
464	V	A	0.4985
465	A	A	-0.0110
466	R	A	0.0000
467	T	A	0.0000
468	L	A	0.0000
469	L	A	0.5374
470	V	A	0.4782
471	F	A	0.0000
472	E	A	-0.3487
473	V	A	0.0000
474	Q	A	0.0000
475	Q	A	0.0000
476	P	A	0.0000
477	F	A	0.0000
478	L	A	0.0000
479	F	A	0.0000
480	V	A	0.0000
481	L	A	0.0000
482	W	A	0.0000
483	D	A	0.0000
484	Q	A	-2.3498
485	Q	A	-2.2831
486	H	A	-1.8136
487	K	A	-2.2855
488	F	A	0.0000
489	P	A	0.0000
490	V	A	0.0000
491	F	A	0.0000
492	M	A	0.0000
493	G	A	0.0000
494	R	A	0.0000
495	V	A	0.0000
496	Y	A	0.0000
497	D	A	-2.3987
498	P	A	-2.1108
499	R	A	-2.9479
54	G	G	-1.4023
55	D	G	-0.5581
56	V	G	1.0817
57	A	G	1.4675
58	I	G	2.0430
59	A	G	0.7086
60	T	G	-0.6840
61	T	G	-1.6916
62	K	G	-2.2729
63	A	G	-1.9341
64	S	G	-1.9478
65	E	G	-2.5432
66	H	G	-1.7566
67	G	G	-1.4819
68	I	G	-0.6950
69	N	G	-1.2967
70	V	G	0.0000
71	T	G	-1.3619
72	G	G	-1.6002
73	R	G	-2.0193
74	T	G	-0.9323
75	A	G	-1.3221
76	E	G	-2.3289
77	V	G	-1.8180
78	R	G	-2.3608
79	V	G	0.0000
80	T	G	-0.5491
81	G	G	-0.0637
82	G	G	0.8945
83	T	G	0.0000
84	I	G	0.0000
85	R	G	-2.4266
86	T	G	0.0000
87	S	G	-2.2388
88	G	G	0.0000
89	N	G	-2.2238
90	Q	G	-2.4425
91	A	G	0.0000
92	Q	G	-1.5791
93	G	G	0.0000
94	L	G	0.0000
95	R	G	-1.0131
96	V	G	0.0000
97	G	G	0.0000
98	T	G	-1.6398
99	E	G	-2.5549
100	N	G	-2.3736
108	V	G	0.8161
109	A	G	0.0000
110	S	G	-0.6219
111	V	G	0.0000
112	F	G	-1.1927
113	L	G	0.0000
114	Q	G	-1.8019
115	N	G	-1.8231
116	L	G	0.0000
117	I	G	-0.1865
118	I	G	0.0000
119	E	G	-2.6025
120	T	G	0.0000
121	S	G	-2.1880
122	G	G	-1.8035
123	T	G	-1.7998
124	G	G	-1.5102
125	A	G	0.0000
126	L	G	-1.1639
127	G	G	0.0000
128	V	G	0.0000
129	S	G	0.0000
130	V	G	0.0000
131	H	G	-0.9422
132	E	G	-1.3663
133	P	G	-1.8353
134	Q	G	-2.0817
135	G	G	-1.5209
136	G	G	-0.9989
137	G	G	-0.9896
138	G	G	-0.7669
139	T	G	0.0000
140	R	G	-0.6971
141	L	G	0.0000
142	S	G	-0.5959
143	M	G	0.0000
144	S	G	-1.7129
145	G	G	-1.4814
146	T	G	0.0000
147	T	G	-0.8226
148	V	G	0.0000
149	R	G	-2.5793
150	T	G	0.0000
151	R	G	-2.9092
152	G	G	-2.1735
153	D	G	-2.1492
154	D	G	-1.7897
155	S	G	0.0000
156	F	G	-0.5329
157	A	G	0.0000
158	L	G	0.0000
159	Q	G	-0.1799
160	L	G	0.0000
161	S	G	-0.8363
162	G	G	0.0000
163	P	G	-1.4291
164	A	G	0.0000
165	S	G	0.0000
166	A	G	0.0000
167	T	G	-0.8395
168	L	G	0.0000
169	N	G	-2.0332
170	D	G	-2.3476
171	V	G	0.0000
172	A	G	-0.7336
173	L	G	0.0000
174	E	G	-1.4096
175	T	G	0.0000
176	A	G	-1.8718
177	G	G	0.0000
178	Q	G	-2.2714
179	Q	G	-1.4271
180	A	G	0.0000
181	P	G	0.0000
182	A	G	0.0000
183	V	G	0.0000
184	V	G	0.0000
185	L	G	0.0000
186	W	G	-0.7048
187	Q	G	-1.4826
188	G	G	-1.3287
189	A	G	0.0000
190	Q	G	-1.7709
191	L	G	0.0000
192	N	G	-1.4166
193	A	G	0.0000
194	Q	G	-2.0426
195	G	G	-1.5149
196	L	G	0.0000
197	V	G	-0.1377
198	V	G	0.0000
199	Q	G	-0.6858
200	V	G	0.0000
201	N	G	-1.4115
202	G	G	-1.1902
203	A	G	-0.7673
204	G	G	0.0000
205	V	G	-0.0161
206	S	G	0.0000
207	A	G	0.0000
208	I	G	0.0000
209	H	G	0.0000
210	A	G	0.0000
211	Q	G	-0.7252
212	D	G	-1.1194
213	A	G	-1.0859
214	G	G	0.0000
215	S	G	-1.2975
216	F	G	0.0000
217	T	G	-0.9014
218	L	G	0.0000
219	S	G	-1.2817
220	G	G	-1.2963
221	S	G	0.0000
222	D	G	-1.1981
223	I	G	0.0000
224	T	G	-0.8454
225	A	G	0.0000
226	R	G	-1.5316
227	G	G	-0.9886
228	L	G	-0.1509
229	E	G	-0.4958
230	V	G	0.0000
231	V	G	0.0000
232	G	G	0.0000
233	I	G	0.0000
234	Y	G	0.0000
235	V	G	0.0000
236	Q	G	0.0000
237	E	G	-1.2206
238	G	G	-1.2608
239	M	G	0.0000
240	Q	G	-1.5319
241	G	G	0.0000
242	T	G	-0.5939
243	L	G	0.0000
244	T	G	-0.9475
245	G	G	-1.1052
246	T	G	0.0000
247	R	G	-1.7176
248	V	G	0.0000
249	T	G	-0.9995
250	T	G	0.0000
251	Q	G	-1.7737
252	G	G	0.0000
253	D	G	-1.9010
254	T	G	-0.8552
255	A	G	0.0000
256	P	G	0.0000
257	A	G	0.0000
258	L	G	0.0000
259	Q	G	0.0000
260	V	G	0.0000
261	E	G	0.0000
262	D	G	-1.2540
263	A	G	-1.1033
264	G	G	-1.0618
265	T	G	0.0000
266	H	G	-1.1991
267	V	G	0.0000
268	S	G	-0.9431
269	M	G	0.0000
270	N	G	-1.6395
271	G	G	-1.5481
272	G	G	0.0000
273	A	G	-0.7567
274	L	G	0.0000
275	S	G	-0.4233
276	T	G	0.0000
277	S	G	-0.9262
278	G	G	-1.3010
279	A	G	-1.5265
280	N	G	-1.2732
281	S	G	0.0000
282	P	G	0.0000
283	A	G	0.0000
284	A	G	0.0000
285	W	G	0.0000
286	L	G	0.0000
287	L	G	0.0000
288	A	G	-0.7846
289	G	G	-1.1884
290	G	G	0.0000
291	S	G	-1.4040
292	A	G	0.0000
293	Q	G	-1.3699
294	F	G	0.0000
295	R	G	-2.9339
296	D	G	-2.4781
297	T	G	0.0000
298	V	G	-0.4740
299	L	G	0.0000
300	R	G	-0.4386
301	T	G	0.0000
302	V	G	0.2526
303	G	G	0.0000
304	E	G	-2.6195
305	A	G	-1.5610
306	S	G	0.0000
307	H	G	-0.5834
308	G	G	0.0000
309	V	G	0.0000
310	D	G	-0.2522
311	V	G	0.0000
312	A	G	-0.6147
313	A	G	-0.8786
314	H	G	-1.5829
315	S	G	0.0000
316	E	G	-2.3582
317	V	G	0.0000
318	E	G	-1.5078
319	L	G	0.0000
320	A	G	-2.0362
321	H	G	-2.2382
322	A	G	0.0000
323	Q	G	-0.8442
324	V	G	0.0000
325	R	G	-0.5998
326	A	G	0.0000
327	D	G	0.0000
328	G	G	-1.4216
329	Q	G	-2.4617
330	G	G	-2.1247
331	A	G	0.0000
332	H	G	-0.7804
333	G	G	0.0000
334	L	G	0.0000
335	V	G	0.0000
336	V	G	0.0000
337	T	G	-0.8686
338	R	G	-1.4033
339	S	G	0.0000
340	S	G	-1.3468
341	A	G	0.0000
342	M	G	-0.6594
343	V	G	0.0000
344	R	G	-1.5805
345	A	G	-1.4000
346	G	G	0.0000
347	S	G	-0.6088
348	L	G	-0.2110
349	V	G	0.0000
350	E	G	-0.7037
351	S	G	0.0000
352	T	G	-1.3349
353	G	G	-1.8118
354	D	G	-2.5675
355	G	G	-1.3490
356	A	G	0.0000
357	A	G	0.0000
358	A	G	0.0000
359	L	G	-0.1809
360	L	G	0.0000
361	E	G	-1.2261
362	S	G	-1.4817
363	G	G	0.0000
364	H	G	-1.6959
365	L	G	0.0000
366	T	G	-0.4950
367	V	G	0.0000
368	D	G	-1.4487
369	G	G	-1.4430
370	S	G	0.0000
371	V	G	-0.3918
372	V	G	0.0000
373	H	G	-0.9311
374	G	G	0.0000
375	H	G	-1.6509
376	G	G	-1.5497
377	A	G	-1.4980
378	A	G	0.0000
379	G	G	0.0000
380	L	G	0.0000
381	E	G	-0.4045
382	V	G	0.0000
383	D	G	-1.0218
384	G	G	-1.4564
385	E	G	-2.7422
386	S	G	0.0000
387	N	G	-1.7308
388	V	G	0.0000
389	S	G	-0.6984
390	L	G	0.0000
391	L	G	-2.0786
392	N	G	-2.6000
393	G	G	-2.0884
394	A	G	0.0000
395	R	G	-1.9414
396	L	G	0.0000
397	S	G	-1.2595
398	S	G	0.0000
399	D	G	-2.5867
400	Q	G	-2.2581
401	P	G	-1.3101
402	T	G	-0.8815
403	A	G	0.0000
404	I	G	0.0000
405	R	G	-0.8152
406	L	G	0.0000
407	I	G	-0.7431
408	D	G	-1.4696
409	P	G	-2.3996
410	R	G	-3.5135
411	S	G	0.0000
412	V	G	-2.4327
413	L	G	0.0000
414	N	G	-2.2002
415	L	G	0.0000
416	D	G	-1.1095
417	I	G	0.0000
418	K	G	-2.7258
419	D	G	-3.4445
420	R	G	-3.6746
421	A	G	0.0000
422	Q	G	-1.3628
423	L	G	0.0000
424	L	G	-0.6479
425	G	G	0.0000
426	D	G	0.0000
427	I	G	0.0000
428	A	G	-1.1661
429	P	G	0.0000
430	E	G	-2.0342
431	A	G	-1.4542
432	Q	G	-2.1302
433	Q	G	-2.3804
434	P	G	-2.2898
435	E	G	-3.2474
436	Q	G	-2.9543
437	A	G	-3.0581
438	R	G	-3.4336
439	V	G	0.0000
440	R	G	-2.7002
441	V	G	0.0000
442	A	G	-1.2007
443	L	G	0.0000
444	A	G	-2.9977
445	D	G	-3.7683
446	G	G	-2.9681
447	G	G	0.0000
448	T	G	-0.9409
449	W	G	0.0000
450	A	G	0.3788
451	G	G	0.0000
452	R	G	-1.5416
453	T	G	0.0000
454	D	G	-2.4234
455	G	G	-2.2189
456	V	G	0.0000
457	H	G	-2.3499
458	T	G	0.0000
459	V	G	0.0000
460	R	G	-1.6784
461	L	G	0.0000
462	L	G	-2.0415
463	D	G	-3.2383
464	R	G	-3.5220
465	G	G	-1.7277
466	V	G	0.5208
467	W	G	0.6497
468	T	G	0.7194
469	V	G	0.6962
470	T	G	-0.2929
471	G	G	-1.5051
472	D	G	-2.5539
473	S	G	-2.2588
474	R	G	-2.9772
475	V	G	-1.3839
476	A	G	-1.2736
477	E	G	-1.5882
478	V	G	-0.0694
479	K	G	-1.0893
480	L	G	-0.2271
481	E	G	-2.2873

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Automated mutations analysis

In the automated mutations mode, the server selects aggregation prone resides and each selected residue is mutated to glutamic acid, lysine, aspartic acid and arginine. The table below shows 2 best scored mutants for each mutated residue. Protein variants are ordered according to the mutation effect they had on protein stability (energetic effect) together with the difference in the average per-residue aggregation score between the wild type and the mutant (in the table green values indicate a positive change, grey are neutral, and orange/red mean destabilizing or more aggregation prone mutants).
Summary for all the mutants can be found in this CSV file.

Mutant	Energetic effect	Score comparison
VR56G	-0.8952	-0.0145	View	CSV	PDB
AR57G	-1.3506	-0.0089	View	CSV	PDB
AK57G	-1.4075	-0.0083	View	CSV	PDB
VK56G	-0.4429	-0.0139	View	CSV	PDB
LE129A	-0.3419	-0.0107	View	CSV	PDB
IK58G	-0.1414	-0.0137	View	CSV	PDB
IR58G	-0.0293	-0.0154	View	CSV	PDB
LK129A	-0.0934	-0.0085	View	CSV	PDB
FD122A	-0.0705	-0.0088	View	CSV	PDB
TE128A	-0.2202	-0.0052	View	CSV	PDB
FR122A	-0.0486	-0.0054	View	CSV	PDB
TR128A	-0.2197	-0.0032	View	CSV	PDB

Project name: 78d5b6ad0a2f989

Status: done

Started: 2025-02-07 04:19:47

Automated mutations analysis

Mutant

Energetic effect

Score comparison