Project name: HY0001

Chain sequence(s)	H: EVQLVESGGGLVQPGRSLRLSCTTSGLTFTHYHMSWVRQAPGKGLEWVAIIRNKAYGYTTDYSASVRGRFTISRDDSKSILYLQMNSLKTEDTAVYYCVREGGYGYDGTFAYWGQGTLVTVSS; L: DIQMTQSPSSLSASVGDRVTITCRASESVDNYGISFMNWFQQKPGQAPKLLIYAASNQGSGVPSRFSGSGSGTDFTLTISSMQPEDFATYYCQQSKEVPRTFGGGTKVEIK
Selected Chain(s)	H,L
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:02)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:02)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:02)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:02)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:02)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:01:46)
[INFO]       Main:     Simulation completed successfully.                                          (00:01:48)

• Minimal score value: -2.914
• Maximal score value: 1.5468
• Average score: -0.5647
• Total score value: -132.1402

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	H	-1.9833
2	V	H	-0.9041
3	Q	H	-0.9155
4	L	H	0.0000
5	V	H	0.7902
6	E	H	0.0000
7	S	H	-0.2453
8	G	H	-0.4980
9	G	H	0.1620
11	G	H	0.8113
12	L	H	1.4379
13	V	H	0.0000
14	Q	H	-1.2783
15	P	H	-2.0317
16	G	H	-2.1119
17	R	H	-2.6378
18	S	H	-1.7740
19	L	H	-1.1211
20	R	H	-1.7077
21	L	H	0.0000
22	S	H	-0.2936
23	C	H	0.0000
24	T	H	0.0173
25	T	H	0.0000
26	S	H	-0.7693
27	G	H	-1.0768
28	L	H	-0.4454
29	T	H	-0.3220
30	F	H	0.0000
35	T	H	-1.3231
36	H	H	-1.3609
37	Y	H	-0.6819
38	H	H	-0.6067
39	M	H	0.0000
40	S	H	0.0000
41	W	H	0.0000
42	V	H	0.0000
43	R	H	0.0000
44	Q	H	-0.6321
45	A	H	-1.0384
46	P	H	-0.8003
47	G	H	-1.4581
48	K	H	-2.2685
49	G	H	-1.4536
50	L	H	0.0000
51	E	H	-0.9701
52	W	H	0.0000
53	V	H	0.0000
54	A	H	0.0000
55	I	H	0.0000
56	I	H	0.0000
57	R	H	-0.8007
58	N	H	-1.1832
59	K	H	-1.7551
60	A	H	-0.4906
61	Y	H	0.3638
62	G	H	-0.3917
63	Y	H	-0.3529
64	T	H	-0.2229
65	T	H	-0.1891
66	D	H	-0.3826
67	Y	H	-0.4747
68	S	H	0.0000
69	A	H	-0.7593
70	S	H	-0.9131
71	V	H	0.0000
72	R	H	-1.9375
74	G	H	-1.4620
75	R	H	-1.4630
76	F	H	0.0000
77	T	H	-0.8944
78	I	H	0.0000
79	S	H	-0.3118
80	R	H	-0.6819
81	D	H	-1.1352
82	D	H	-1.5806
83	S	H	-1.3205
84	K	H	-2.0048
85	S	H	-1.1145
86	I	H	-0.4179
87	L	H	0.0000
88	Y	H	-0.4256
89	L	H	0.0000
90	Q	H	-1.1654
91	M	H	0.0000
92	N	H	-1.8007
93	S	H	-1.7718
94	L	H	0.0000
95	K	H	-2.6573
96	T	H	-1.8221
97	E	H	-2.3637
98	D	H	0.0000
99	T	H	-0.4679
100	A	H	0.0000
101	V	H	0.7431
102	Y	H	0.0000
103	Y	H	0.0000
104	C	H	0.0000
105	V	H	0.0000
106	R	H	0.0000
107	E	H	0.1253
108	G	H	0.0000
109	G	H	0.3840
110	Y	H	1.2610
111	G	H	0.5152
112A	Y	H	0.9390
112	D	H	0.4280
113	G	H	0.0981
114	T	H	0.0000
115	F	H	0.0000
116	A	H	0.1947
117	Y	H	0.4619
118	W	H	-0.0363
119	G	H	0.0000
120	Q	H	-1.1160
121	G	H	-0.1844
122	T	H	0.5507
123	L	H	1.5468
124	V	H	0.0000
125	T	H	0.3249
126	V	H	0.0000
127	S	H	-0.5006
128	S	H	-0.3489
1	D	L	-2.4719
2	I	L	0.0000
3	Q	L	-2.4386
4	M	L	0.0000
5	T	L	-1.4307
6	Q	L	-1.1694
7	S	L	-0.7298
8	P	L	-0.5458
9	S	L	-0.7819
10	S	L	-0.9678
11	L	L	-0.7141
12	S	L	-0.8765
13	A	L	0.0000
14	S	L	-0.8143
15	V	L	0.0757
16	G	L	-0.6812
17	D	L	-1.7708
18	R	L	-2.3114
19	V	L	0.0000
20	T	L	-0.6239
21	I	L	0.0000
22	T	L	-0.8881
23	C	L	0.0000
24	R	L	-2.9140
25	A	L	0.0000
26	S	L	-2.3465
27	E	L	-2.7792
28	S	L	-1.7099
29	V	L	0.0000
30	D	L	-0.8301
31	N	L	-0.2321
34	Y	L	0.9172
35	G	L	0.1701
36	I	L	0.4461
37	S	L	-0.1478
38	F	L	-0.0964
39	M	L	0.0000
40	N	L	0.0000
41	W	L	0.0000
42	F	L	0.0000
43	Q	L	0.0000
44	Q	L	-0.9885
45	K	L	-1.3371
46	P	L	-0.9270
47	G	L	-1.4223
48	Q	L	-2.0077
49	A	L	-1.2383
50	P	L	0.0000
51	K	L	-1.0274
52	L	L	0.0000
53	L	L	0.0000
54	I	L	0.0000
55	Y	L	-0.0611
56	A	L	-0.1113
57	A	L	0.0000
65	S	L	-0.6782
66	N	L	-0.8783
67	Q	L	-1.1536
68	G	L	0.0000
69	S	L	-0.7074
70	G	L	-0.6442
71	V	L	-0.6303
72	P	L	-0.4950
74	S	L	-0.6033
75	R	L	-0.8363
76	F	L	0.0000
77	S	L	-0.6322
78	G	L	-0.5905
79	S	L	-0.7428
80	G	L	-1.0968
83	S	L	-1.0290
84	G	L	-1.3133
85	T	L	-1.9480
86	D	L	-2.0651
87	F	L	0.0000
88	T	L	-0.7745
89	L	L	0.0000
90	T	L	-0.5970
91	I	L	0.0000
92	S	L	-1.2451
93	S	L	-1.1175
94	M	L	0.0000
95	Q	L	-0.8543
96	P	L	-0.9625
97	E	L	-1.5946
98	D	L	0.0000
99	F	L	-0.3239
100	A	L	0.0000
101	T	L	-0.7972
102	Y	L	0.0000
103	Y	L	0.0000
104	C	L	0.0000
105	Q	L	0.0000
106	Q	L	0.0000
107	S	L	0.0000
108	K	L	-1.0865
109	E	L	-1.5528
114	V	L	-0.1438
115	P	L	0.0000
116	R	L	-0.8302
117	T	L	-0.9353
118	F	L	0.0000
119	G	L	0.0000
120	G	L	-1.2276
121	G	L	-0.9892
122	T	L	0.0000
123	K	L	-1.5141
124	V	L	0.0000
125	E	L	-1.2710
126	I	L	-0.5605
127	K	L	-1.4795