Project name: GS linker_12

Chain sequence(s)	A: GGGGSGGGGSGGGGS; C: GGGGSGGGGSGGGGS; B: GGGGSGGGGSGGGGS; E: GGGGSGGGGSGGGGS; D: GGGGSGGGGSGGGGS; G: GGGGSGGGGSGGGGS; F: GGGGSGGGGSGGGGS; I: GGGGSGGGGSGGGGS; H: GGGGSGGGGSGGGGS; K: GGGGSGGGGSGGGGS; J: GGGGSGGGGSGGGGS; L: GGGGSGGGGSGGGGS
Selected Chain(s)	A,C,B,E,D,G,F,I,H,K,J,L
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:00:06)
[INFO]       Main:     Simulation completed successfully.                                          (00:00:09)

• Minimal score value: -1.9814
• Maximal score value: 0.0
• Average score: -1.2757
• Total score value: -229.6233

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	G	A	-1.4231
2	G	A	-1.6492
3	G	A	0.0000
4	G	A	0.0000
5	S	A	0.0000
6	G	A	-1.2124
7	G	A	-1.3118
8	G	A	-1.3016
9	G	A	-1.3918
10	S	A	-1.5068
11	G	A	-1.8655
12	G	A	-1.9814
13	G	A	-1.8118
14	G	A	-1.4962
15	S	A	-0.9601
1	G	B	-1.3691
2	G	B	0.0000
3	G	B	-1.3201
4	G	B	-1.2241
5	S	B	0.0000
6	G	B	-1.2528
7	G	B	-1.3233
8	G	B	-1.3574
9	G	B	-1.5660
10	S	B	-1.5300
11	G	B	-1.7269
12	G	B	-1.8177
13	G	B	-1.6433
14	G	B	-1.3015
15	S	B	-0.8138
1	G	C	-1.2928
2	G	C	0.0000
3	G	C	-1.2609
4	G	C	-1.1639
5	S	C	0.0000
6	G	C	-1.0980
7	G	C	-1.2399
8	G	C	-1.4263
9	G	C	-1.4776
10	S	C	-1.4600
11	G	C	-1.6549
12	G	C	-1.6390
13	G	C	-1.5307
14	G	C	-1.1726
15	S	C	-0.7744
1	G	D	-1.1865
2	G	D	-1.3120
3	G	D	-1.2063
4	G	D	-1.1343
5	S	D	-1.0772
6	G	D	-1.0734
7	G	D	-1.2156
8	G	D	-1.4103
9	G	D	-1.4846
10	S	D	-1.4763
11	G	D	-1.5194
12	G	D	-1.4002
13	G	D	-1.4231
14	G	D	-1.0432
15	S	D	-0.6270
1	G	E	-1.1589
2	G	E	-1.3169
3	G	E	-1.1760
4	G	E	-1.4402
5	S	E	0.0000
6	G	E	-1.2235
7	G	E	-1.3305
8	G	E	-1.3138
9	G	E	-1.3759
10	S	E	-1.3293
11	G	E	-1.5430
12	G	E	-1.3787
13	G	E	-1.2439
14	G	E	-1.0215
15	S	E	-0.6112
1	G	F	-1.2382
2	G	F	-1.5850
3	G	F	-1.3927
4	G	F	-1.6006
5	S	F	0.0000
6	G	F	0.0000
7	G	F	-1.4092
8	G	F	-1.3285
9	G	F	-1.3179
10	S	F	-1.2762
11	G	F	-1.4831
12	G	F	-1.4509
13	G	F	-1.2805
14	G	F	-1.0648
15	S	F	-0.6325
1	G	G	-1.0656
2	G	G	-1.4320
3	G	G	-1.3972
4	G	G	-1.5402
5	S	G	0.0000
6	G	G	-1.3408
7	G	G	-1.3800
8	G	G	-1.2659
9	G	G	-1.3082
10	S	G	-1.3254
11	G	G	-1.7110
12	G	G	-1.8951
13	G	G	-1.6719
14	G	G	-1.6389
15	S	G	-0.8976
1	G	H	-1.2258
2	G	H	-1.7211
3	G	H	-1.6143
4	G	H	-1.7184
5	S	H	0.0000
6	G	H	-1.4137
7	G	H	-1.3866
8	G	H	-1.1948
9	G	H	-1.4154
10	S	H	-1.4850
11	G	H	-1.5498
12	G	H	-1.8808
13	G	H	-1.5174
14	G	H	-1.2537
15	S	H	-0.8280
1	G	I	-1.2383
2	G	I	-1.5679
3	G	I	-1.7454
4	G	I	-1.6380
5	S	I	-1.4135
6	G	I	-1.3484
7	G	I	-1.3355
8	G	I	-1.3652
9	G	I	-1.3425
10	S	I	-1.4356
11	G	I	-1.8530
12	G	I	-1.7892
13	G	I	-1.8528
14	G	I	-1.3435
15	S	I	-0.8633
1	G	J	-1.2437
2	G	J	-1.6689
3	G	J	-1.6284
4	G	J	-1.5041
5	S	J	-1.3396
6	G	J	-1.2344
7	G	J	-1.2126
8	G	J	-1.2706
9	G	J	-1.2449
10	S	J	-1.1798
11	G	J	-1.6825
12	G	J	-1.8012
13	G	J	-1.7319
14	G	J	-1.4805
15	S	J	-0.8903
1	G	K	-1.2337
2	G	K	-1.6636
3	G	K	-1.5940
4	G	K	-1.5193
5	S	K	0.0000
6	G	K	-1.2557
7	G	K	-1.2632
8	G	K	-1.2303
9	G	K	-1.2316
10	S	K	-1.2567
11	G	K	-1.7769
12	G	K	-1.9263
13	G	K	-1.8048
14	G	K	-1.4357
15	S	K	-0.8337
1	G	L	-1.1594
2	G	L	-1.4233
3	G	L	-1.4445
4	G	L	-1.4830
5	S	L	0.0000
6	G	L	-1.2597
7	G	L	-1.2950
8	G	L	-1.1891
9	G	L	-1.3642
10	S	L	-1.4084
11	G	L	-1.6699
12	G	L	-1.9703
13	G	L	-1.7803
14	G	L	-1.4448
15	S	L	-0.9243