Project name: IL10_WT_automut

Chain sequence(s)	L: CTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTM
Selected Chain(s)	L
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with L chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:03:19)
[INFO]       Main:     Simulation completed successfully.                                          (00:03:20)

• Minimal score value: -3.9831
• Maximal score value: 2.6837
• Average score: -1.1022
• Total score value: -159.8146

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
12	C	L	-0.4701
13	T	L	-0.5503
14	H	L	-1.4606
15	F	L	-0.8439
16	P	L	-0.7918
17	G	L	-1.2778
18	N	L	-1.5619
19	L	L	-1.3007
20	P	L	-1.7035
21	N	L	-2.5443
22	M	L	-2.2310
23	L	L	-2.3096
24	R	L	-3.9831
25	D	L	-3.7985
26	L	L	-2.7890
27	R	L	-3.7408
28	D	L	-3.9677
29	A	L	0.0000
30	F	L	-1.9057
31	S	L	-2.0796
32	R	L	-2.6440
33	V	L	0.0000
34	K	L	-1.8124
35	T	L	-0.9935
36	F	L	-0.8144
37	F	L	-1.0814
38	Q	L	-1.7893
39	M	L	-0.9569
40	K	L	-2.2696
41	D	L	-2.8542
42	Q	L	-2.2483
43	L	L	-0.7929
44	D	L	-1.7806
45	N	L	-1.0828
46	L	L	0.4221
47	L	L	1.3119
48	L	L	0.6006
49	K	L	-1.5851
50	E	L	-2.2132
51	S	L	-1.9559
52	L	L	-0.9394
53	L	L	-1.0953
54	E	L	-2.5211
55	D	L	-1.4046
56	F	L	-0.6194
57	K	L	-1.5951
58	G	L	-0.6152
59	Y	L	0.8401
60	L	L	0.5809
61	G	L	-0.1003
62	C	L	-0.3665
63	Q	L	-1.0100
64	A	L	0.0000
65	L	L	-0.1622
66	S	L	-1.0944
67	E	L	-1.8363
68	M	L	-0.8224
69	I	L	0.0000
70	Q	L	-1.4444
71	F	L	-0.7337
72	Y	L	-0.4799
73	L	L	-1.6066
74	E	L	-2.3177
75	E	L	-2.1208
76	V	L	-0.6982
77	M	L	0.0000
78	P	L	-2.0971
79	Q	L	-2.5823
80	A	L	-2.1958
81	E	L	0.0000
82	N	L	-3.1603
83	Q	L	-2.9726
84	D	L	-2.8497
85	P	L	-2.6215
86	D	L	-2.7815
87	I	L	0.0000
88	K	L	-2.9150
89	A	L	-1.7479
90	H	L	-1.8584
91	V	L	0.0000
92	N	L	-2.2918
93	S	L	-1.7470
94	L	L	-1.9768
95	G	L	0.0000
96	E	L	-2.5859
97	N	L	-2.2711
98	L	L	-1.3231
99	K	L	-1.8294
100	T	L	-1.2715
101	L	L	0.0000
102	R	L	-1.0834
103	L	L	-0.7517
104	R	L	-1.1621
105	L	L	0.0000
106	R	L	-2.1155
107	R	L	-2.5992
108	C	L	0.0000
109	H	L	-2.6006
110	R	L	-2.3940
111	F	L	-0.9562
112	L	L	0.0000
113	P	L	-1.7517
114	C	L	-0.8374
115	E	L	-1.4361
116	N	L	-2.5355
117	K	L	-2.5025
118	S	L	-1.8039
119	K	L	-2.3881
120	A	L	-1.0701
121	V	L	-0.5759
122	E	L	-1.7610
123	Q	L	-1.6951
124	V	L	-0.8471
125	K	L	-2.1436
126	N	L	-2.1850
127	A	L	-1.4002
128	F	L	-1.4521
129	N	L	-2.7700
130	K	L	-2.8206
131	L	L	-1.8985
132	Q	L	-2.5780
133	E	L	-2.6030
134	K	L	-2.1821
135	G	L	0.0000
136	I	L	1.3212
137	Y	L	0.7634
138	K	L	-0.3719
139	A	L	0.9758
140	M	L	1.8136
141	S	L	0.4766
142	E	L	-0.0502
143	F	L	1.8634
144	D	L	0.4417
145	I	L	2.0696
146	F	L	2.6837
147	I	L	1.3284
148	N	L	0.3644
149	Y	L	2.0317
150	I	L	1.3688
151	E	L	0.0044
152	A	L	0.8491
153	Y	L	1.9452
154	M	L	1.5998
155	T	L	1.1437
156	M	L	1.5542