Chain sequence(s) |
A: MVERNSGDLQLPQEIAMLVPNKTQVVPKSGGEGKVKDIFASPALVRAGGVMIAFVEGRTKNKLFPEVIDLSSSDIVAGYIKAPETWQSLVAEVTKEYWQAHTVLESANNSNHRVGVARLPTGITRGNKVFLLVGSYEERREIDDYIWKAEAWNIKVIEGEATQSTEVQPTQPINWSEPKPLFQTDSPNNKGDLKEFLGGGGSGIVMGNGTLVFPLTAKDESNKVFSLITYSTDDGQKWEIPGGVSSVASRSPRVTEWEEGTLLMVTYSEDGRKVFESRDMGKTWTEAFGTLPGVWLKSGPELPEVSLRVDALITATIEGRKVMLYTQKVRHFLEVDEPNALHLWVTDNNRTFHLGPFSVDSAENKTFANTLLYSDDALHLLQAKGDHESTAVSLARLTEELNTINSVLSTWVQLDASFSESSIPTAGLVGFLSNTTSSGDTWIDGYRSMNATVTKAAKVENGFKFTGPGSRATWPVNSRWDIKQYGFVDYNFTIVAMATIHQVPSESTPLLGASLRGNKRTKLIGLSYGAGGKWETVYDGTKTVQGGTWEPGREYQVALMLQDGNKGFVYVDGVLVGNPAMLPTPEERWTEFSHFYFGGDEGDSGSDATLTDVFLYNRPLSVGELKMIKEVED
input PDB |
Selected Chain(s) | A |
Distance of aggregation | 10 Å |
FoldX usage | Yes |
Dynamic mode | No |
Automated mutations | Yes |
Downloads | Download all the data |
Simulation log |
[INFO] Logger: Verbosity set to: 2 - [INFO] (00:00:00) [WARNING] runJob: Working directory already exists (possibly overwriting previous results -ow to prevent this behavior) (00:00:00) [INFO] runJob: Starting aggrescan3d job on: input.pdb with A chain(s) selected (00:00:00) [INFO] runJob: Creating pdb object from: input.pdb (00:00:00) [INFO] FoldX: Starting FoldX energy minimalization (00:00:00) [INFO] Analysis: Starting Aggrescan3D on folded.pdb (00:09:56) [INFO] Auto_mut: Residue number 574 from chain A and a score of 2.012 (valine) selected for automated muatation (00:10:01) [INFO] Auto_mut: Residue number 288 from chain A and a score of 1.634 (phenylalanine) selected for automated muatation (00:10:01) [INFO] Auto_mut: Residue number 332 from chain A and a score of 1.471 (phenylalanine) selected for automated muatation (00:10:01) [INFO] Auto_mut: Residue number 333 from chain A and a score of 1.437 (leucine) selected for automated muatation (00:10:01) [INFO] Auto_mut: Residue number 1 from chain A and a score of 1.154 omitted from automated muatation (excluded by the user). (00:10:01) [INFO] Auto_mut: Residue number 575 from chain A and a score of 1.112 (leucine) selected for automated muatation (00:10:01) [INFO] Auto_mut: Residue number 622 from chain A and a score of 1.079 (valine) selected for automated muatation (00:10:01) [INFO] Auto_mut: Mutating residue number 574 from chain A (valine) into glutamic acid (00:10:01) [INFO] Auto_mut: Mutating residue number 574 from chain A (valine) into aspartic acid (00:10:01) [INFO] Auto_mut: Mutating residue number 288 from chain A (phenylalanine) into glutamic acid Mutating residue number 288 from chain A (phenylalanine) into glutamic acid (00:10:01) [INFO] Auto_mut: Mutating residue number 574 from chain A (valine) into arginine (00:13:55) [INFO] Auto_mut: Mutating residue number 574 from chain A (valine) into lysine (00:13:58) [INFO] Auto_mut: Mutating residue number 288 from chain A (phenylalanine) into lysine (00:14:24) [INFO] Auto_mut: Mutating residue number 288 from chain A (phenylalanine) into aspartic acid Mutating residue number 288 from chain A (phenylalanine) into aspartic acid (00:18:04) [INFO] Auto_mut: Mutating residue number 332 from chain A (phenylalanine) into glutamic acid Mutating residue number 332 from chain A (phenylalanine) into glutamic acid (00:18:13) [INFO] Auto_mut: Mutating residue number 332 from chain A (phenylalanine) into aspartic acid Mutating residue number 332 from chain A (phenylalanine) into aspartic acid (00:18:25) [INFO] Auto_mut: Mutating residue number 288 from chain A (phenylalanine) into arginine (00:21:53) [INFO] Auto_mut: Mutating residue number 332 from chain A (phenylalanine) into lysine (00:22:05) [INFO] Auto_mut: Mutating residue number 332 from chain A (phenylalanine) into arginine (00:22:22) [INFO] Auto_mut: Mutating residue number 333 from chain A (leucine) into glutamic acid (00:25:54) [INFO] Auto_mut: Mutating residue number 333 from chain A (leucine) into aspartic acid (00:26:14) [INFO] Auto_mut: Mutating residue number 575 from chain A (leucine) into glutamic acid (00:26:14) [INFO] Auto_mut: Mutating residue number 333 from chain A (leucine) into lysine (00:29:35) [INFO] Auto_mut: Mutating residue number 333 from chain A (leucine) into arginine (00:30:01) [INFO] Auto_mut: Mutating residue number 575 from chain A (leucine) into lysine (00:30:03) [INFO] Auto_mut: Mutating residue number 575 from chain A (leucine) into aspartic acid (00:33:29) [INFO] Auto_mut: Mutating residue number 622 from chain A (valine) into glutamic acid (00:33:50) [INFO] Auto_mut: Mutating residue number 622 from chain A (valine) into aspartic acid (00:34:03) [INFO] Auto_mut: Mutating residue number 575 from chain A (leucine) into arginine (00:37:10) [INFO] Auto_mut: Mutating residue number 622 from chain A (valine) into lysine (00:37:34) [INFO] Auto_mut: Mutating residue number 622 from chain A (valine) into arginine (00:37:50) [INFO] Auto_mut: Effect of mutation residue number 574 from chain A (valine) into glutamic acid: Energy difference: -0.4410 kcal/mol, Difference in average score from the base case: -0.0175 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 574 from chain A (valine) into lysine: Energy difference: -0.5435 kcal/mol, Difference in average score from the base case: -0.0182 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 574 from chain A (valine) into aspartic acid: Energy difference: -0.1929 kcal/mol, Difference in average score from the base case: -0.0178 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 574 from chain A (valine) into arginine: Energy difference: -0.7344 kcal/mol, Difference in average score from the base case: -0.0121 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 288 from chain A (phenylalanine) into glutamic acid: Energy difference: 0.8592 kcal/mol, Difference in average score from the base case: -0.0216 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 288 from chain A (phenylalanine) into lysine: Energy difference: 0.4251 kcal/mol, Difference in average score from the base case: -0.0165 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 288 from chain A (phenylalanine) into aspartic acid: Energy difference: 1.0375 kcal/mol, Difference in average score from the base case: -0.0203 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 288 from chain A (phenylalanine) into arginine: Energy difference: -1.2511 kcal/mol, Difference in average score from the base case: -0.0201 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 332 from chain A (phenylalanine) into glutamic acid: Energy difference: 0.8927 kcal/mol, Difference in average score from the base case: -0.0213 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 332 from chain A (phenylalanine) into lysine: Energy difference: 0.1649 kcal/mol, Difference in average score from the base case: -0.0220 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 332 from chain A (phenylalanine) into aspartic acid: Energy difference: 0.0071 kcal/mol, Difference in average score from the base case: -0.0208 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 332 from chain A (phenylalanine) into arginine: Energy difference: -0.2936 kcal/mol, Difference in average score from the base case: -0.0205 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 333 from chain A (leucine) into glutamic acid: Energy difference: 0.2940 kcal/mol, Difference in average score from the base case: -0.0190 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 333 from chain A (leucine) into lysine: Energy difference: 0.0249 kcal/mol, Difference in average score from the base case: -0.0174 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 333 from chain A (leucine) into aspartic acid: Energy difference: 0.4858 kcal/mol, Difference in average score from the base case: -0.0175 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 333 from chain A (leucine) into arginine: Energy difference: -1.3887 kcal/mol, Difference in average score from the base case: -0.0149 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 575 from chain A (leucine) into glutamic acid: Energy difference: 2.1394 kcal/mol, Difference in average score from the base case: -0.0141 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 575 from chain A (leucine) into lysine: Energy difference: 0.8516 kcal/mol, Difference in average score from the base case: -0.0087 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 575 from chain A (leucine) into aspartic acid: Energy difference: 2.4166 kcal/mol, Difference in average score from the base case: -0.0135 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 575 from chain A (leucine) into arginine: Energy difference: 0.6080 kcal/mol, Difference in average score from the base case: -0.0112 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 622 from chain A (valine) into glutamic acid: Energy difference: -0.7187 kcal/mol, Difference in average score from the base case: -0.0190 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 622 from chain A (valine) into lysine: Energy difference: -0.8682 kcal/mol, Difference in average score from the base case: -0.0188 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 622 from chain A (valine) into aspartic acid: Energy difference: 0.3101 kcal/mol, Difference in average score from the base case: -0.0171 (00:41:39) [INFO] Auto_mut: Effect of mutation residue number 622 from chain A (valine) into arginine: Energy difference: -1.1000 kcal/mol, Difference in average score from the base case: -0.0188 (00:41:39) [INFO] Main: Simulation completed successfully. (00:41:50) |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
residue index | residue name | chain | Aggrescan3D score | mutation |
---|---|---|---|---|
residue index | residue name | chain | Aggrescan3D score | |
1 | M | A | 1.1541 | |
2 | V | A | 0.5017 | |
3 | E | A | -1.9856 | |
4 | R | A | -2.9315 | |
5 | N | A | -2.9241 | |
6 | S | A | -2.5456 | |
7 | G | A | -2.3123 | |
8 | D | A | -2.3268 | |
9 | L | A | -1.3924 | |
10 | Q | A | -1.3997 | |
11 | L | A | -0.1599 | |
12 | P | A | -0.8960 | |
13 | Q | A | -1.6405 | |
14 | E | A | -1.9280 | |
15 | I | A | 0.0000 | |
16 | A | A | -0.2015 | |
17 | M | A | 0.0000 | |
18 | L | A | 0.0000 | |
19 | V | A | -0.3090 | |
20 | P | A | -1.1756 | |
21 | N | A | -2.0524 | |
22 | K | A | -2.2981 | |
23 | T | A | 0.0000 | |
24 | Q | A | -1.8482 | |
25 | V | A | 0.0000 | |
26 | V | A | -1.3974 | |
27 | P | A | -1.7149 | |
28 | K | A | -1.5362 | |
29 | S | A | -1.1136 | |
30 | G | A | -1.4378 | |
31 | G | A | -1.9556 | |
32 | E | A | -2.5505 | |
33 | G | A | -2.1717 | |
34 | K | A | -2.2559 | |
35 | V | A | -1.1222 | |
36 | K | A | 0.0000 | |
37 | D | A | -1.3912 | |
38 | I | A | 0.0000 | |
39 | F | A | 0.0000 | |
40 | A | A | -0.0442 | |
41 | S | A | 0.0504 | |
42 | P | A | 0.0000 | |
43 | A | A | 0.0000 | |
44 | L | A | 0.0000 | |
45 | V | A | 0.0000 | |
46 | R | A | -0.7845 | |
47 | A | A | 0.0000 | |
48 | G | A | -1.6360 | |
49 | G | A | -1.6118 | |
50 | V | A | 0.0000 | |
51 | M | A | 0.0000 | |
52 | I | A | 0.0000 | |
53 | A | A | 0.0000 | |
54 | F | A | 0.0000 | |
55 | V | A | 0.0000 | |
56 | E | A | 0.0000 | |
57 | G | A | 0.0000 | |
58 | R | A | -0.7720 | |
59 | T | A | -0.8537 | |
60 | K | A | -1.4125 | |
61 | N | A | -1.4645 | |
62 | K | A | -1.5777 | |
63 | L | A | -0.4748 | |
64 | F | A | -0.6197 | |
65 | P | A | -0.9583 | |
66 | E | A | -1.3626 | |
67 | V | A | 0.2140 | |
68 | I | A | 0.1968 | |
69 | D | A | -1.4129 | |
70 | L | A | 0.0000 | |
71 | S | A | -1.0154 | |
72 | S | A | 0.0000 | |
73 | S | A | 0.0000 | |
74 | D | A | -0.7952 | |
75 | I | A | 0.0000 | |
76 | V | A | 0.0000 | |
77 | A | A | 0.0000 | |
78 | G | A | 0.0000 | |
79 | Y | A | 0.0000 | |
80 | I | A | 0.0000 | |
81 | K | A | -1.3340 | |
82 | A | A | -1.1506 | |
83 | P | A | -1.4982 | |
84 | E | A | -2.6083 | |
85 | T | A | -1.2615 | |
86 | W | A | -1.2015 | |
87 | Q | A | -1.3134 | |
88 | S | A | -1.0636 | |
89 | L | A | 0.0000 | |
90 | V | A | -0.4681 | |
91 | A | A | -0.4342 | |
92 | E | A | -0.7950 | |
93 | V | A | 0.0000 | |
94 | T | A | -0.8054 | |
95 | K | A | -1.5498 | |
96 | E | A | -1.8572 | |
97 | Y | A | -0.4052 | |
98 | W | A | -0.7593 | |
99 | Q | A | -1.1489 | |
100 | A | A | 0.0000 | |
101 | H | A | -0.8089 | |
102 | T | A | 0.0000 | |
103 | V | A | 0.0000 | |
104 | L | A | 0.0000 | |
105 | E | A | -1.6165 | |
106 | S | A | -1.2346 | |
107 | A | A | -1.3093 | |
108 | N | A | -2.4087 | |
109 | N | A | -2.5924 | |
110 | S | A | -2.1871 | |
111 | N | A | -2.2253 | |
112 | H | A | -1.7569 | |
113 | R | A | -2.6345 | |
114 | V | A | 0.0000 | |
115 | G | A | 0.0000 | |
116 | V | A | 0.0000 | |
117 | A | A | 0.0000 | |
118 | R | A | -0.3658 | |
119 | L | A | 0.0111 | |
120 | P | A | 0.0000 | |
121 | T | A | 0.0000 | |
122 | G | A | 0.0000 | |
123 | I | A | 0.0000 | |
124 | T | A | -0.8127 | |
125 | R | A | -1.3317 | |
126 | G | A | -1.4187 | |
127 | N | A | -1.6962 | |
128 | K | A | -1.9460 | |
129 | V | A | 0.0000 | |
130 | F | A | 0.0000 | |
131 | L | A | 0.0000 | |
132 | L | A | 0.0000 | |
133 | V | A | 0.0000 | |
134 | G | A | 0.0000 | |
135 | S | A | -0.5961 | |
136 | Y | A | -0.9148 | |
137 | E | A | -1.7606 | |
138 | E | A | -1.9162 | |
139 | R | A | -1.9516 | |
140 | R | A | -1.1586 | |
141 | E | A | -0.9221 | |
142 | I | A | 0.6401 | |
143 | D | A | -1.4262 | |
144 | D | A | -1.2987 | |
145 | Y | A | 0.7372 | |
146 | I | A | 0.7049 | |
147 | W | A | -0.4554 | |
148 | K | A | -1.7171 | |
149 | A | A | -1.5754 | |
150 | E | A | -2.0542 | |
151 | A | A | -0.9671 | |
152 | W | A | -0.8911 | |
153 | N | A | -1.1942 | |
154 | I | A | 0.0000 | |
155 | K | A | -0.9824 | |
156 | V | A | 0.0000 | |
157 | I | A | 0.0000 | |
158 | E | A | -1.8584 | |
159 | G | A | 0.0000 | |
160 | E | A | -2.2592 | |
161 | A | A | 0.0000 | |
162 | T | A | -1.6634 | |
163 | Q | A | -1.7986 | |
164 | S | A | -1.1790 | |
165 | T | A | -0.9891 | |
166 | E | A | -1.6006 | |
167 | V | A | 0.1975 | |
168 | Q | A | -1.1638 | |
169 | P | A | -1.1114 | |
170 | T | A | -0.5773 | |
171 | Q | A | -0.9224 | |
172 | P | A | 0.0000 | |
173 | I | A | 0.0000 | |
174 | N | A | -1.8152 | |
175 | W | A | -1.5158 | |
176 | S | A | -1.6943 | |
177 | E | A | -2.1848 | |
178 | P | A | -1.4741 | |
179 | K | A | -1.6027 | |
180 | P | A | -1.3770 | |
181 | L | A | 0.0000 | |
182 | F | A | -1.4694 | |
183 | Q | A | -1.8658 | |
184 | T | A | -1.8217 | |
185 | D | A | -2.4680 | |
186 | S | A | -2.3470 | |
187 | P | A | -2.6538 | |
188 | N | A | -3.4504 | |
189 | N | A | -3.2942 | |
190 | K | A | -3.3121 | |
191 | G | A | -3.0966 | |
192 | D | A | -3.5759 | |
193 | L | A | -2.9191 | |
194 | K | A | -2.5775 | |
195 | E | A | -1.2349 | |
196 | F | A | 0.0000 | |
197 | L | A | -0.2260 | |
198 | G | A | 0.0000 | |
199 | G | A | -0.3215 | |
200 | G | A | 0.0000 | |
201 | G | A | 0.0000 | |
202 | S | A | 0.1572 | |
203 | G | A | 0.0000 | |
204 | I | A | 0.4464 | |
205 | V | A | 0.3297 | |
206 | M | A | 0.0000 | |
207 | G | A | -1.5734 | |
208 | N | A | -1.7555 | |
209 | G | A | -0.9850 | |
210 | T | A | 0.0000 | |
211 | L | A | 0.0000 | |
212 | V | A | 0.0000 | |
213 | F | A | 0.0000 | |
214 | P | A | 0.0000 | |
215 | L | A | 0.0000 | |
216 | T | A | -0.4445 | |
217 | A | A | 0.0000 | |
218 | K | A | -2.1870 | |
219 | D | A | -2.8609 | |
220 | E | A | -3.3706 | |
221 | S | A | -2.3405 | |
222 | N | A | -2.7522 | |
223 | K | A | -2.2611 | |
224 | V | A | -1.3951 | |
225 | F | A | -0.4515 | |
226 | S | A | 0.0000 | |
227 | L | A | 0.0000 | |
228 | I | A | 0.0000 | |
229 | T | A | 0.0000 | |
230 | Y | A | -0.4493 | |
231 | S | A | 0.0000 | |
232 | T | A | -1.3144 | |
233 | D | A | -1.5562 | |
234 | D | A | -1.8229 | |
235 | G | A | 0.0000 | |
236 | Q | A | -2.5115 | |
237 | K | A | -2.8769 | |
238 | W | A | -1.8224 | |
239 | E | A | -1.5280 | |
240 | I | A | -0.8381 | |
241 | P | A | 0.0000 | |
242 | G | A | -1.3591 | |
243 | G | A | 0.0000 | |
244 | V | A | 0.3420 | |
245 | S | A | 0.0000 | |
246 | S | A | 0.1237 | |
247 | V | A | -0.2690 | |
248 | A | A | -0.9343 | |
249 | S | A | 0.0000 | |
250 | R | A | -1.8354 | |
251 | S | A | -0.6764 | |
252 | P | A | 0.0000 | |
253 | R | A | 0.0000 | |
254 | V | A | 0.0000 | |
255 | T | A | 0.0000 | |
256 | E | A | -0.9378 | |
257 | W | A | -1.4607 | |
258 | E | A | -2.9147 | |
259 | E | A | -3.2473 | |
260 | G | A | -2.4263 | |
261 | T | A | 0.0000 | |
262 | L | A | 0.0000 | |
263 | L | A | 0.0000 | |
264 | M | A | 0.0000 | |
265 | V | A | 0.0000 | |
266 | T | A | 0.0000 | |
267 | Y | A | -0.8659 | |
268 | S | A | -1.6568 | |
269 | E | A | -2.7388 | |
270 | D | A | -2.6069 | |
271 | G | A | 0.0000 | |
272 | R | A | 0.0000 | |
273 | K | A | -0.4222 | |
274 | V | A | 0.0000 | |
275 | F | A | 0.0000 | |
276 | E | A | -0.9416 | |
277 | S | A | 0.0000 | |
278 | R | A | -2.8756 | |
279 | D | A | -1.8775 | |
280 | M | A | -1.1352 | |
281 | G | A | 0.0000 | |
282 | K | A | -2.0753 | |
283 | T | A | -1.3352 | |
284 | W | A | -0.8525 | |
285 | T | A | -0.4664 | |
286 | E | A | -0.2252 | |
287 | A | A | 0.0000 | |
288 | F | A | 1.6341 | |
289 | G | A | 0.4948 | |
290 | T | A | 0.1668 | |
291 | L | A | 0.0000 | |
292 | P | A | 0.0000 | |
293 | G | A | 0.0000 | |
294 | V | A | 0.0000 | |
295 | W | A | -0.2843 | |
296 | L | A | 0.0000 | |
297 | K | A | -2.3581 | |
298 | S | A | -2.1738 | |
299 | G | A | -1.8035 | |
300 | P | A | -1.8435 | |
301 | E | A | -1.9144 | |
302 | L | A | -1.2018 | |
303 | P | A | -1.2066 | |
304 | E | A | -0.5362 | |
305 | V | A | 0.9103 | |
306 | S | A | 0.4882 | |
307 | L | A | 0.0000 | |
308 | R | A | -0.0790 | |
309 | V | A | 0.0000 | |
310 | D | A | 0.0000 | |
311 | A | A | 0.0000 | |
312 | L | A | 0.0000 | |
313 | I | A | 0.2970 | |
314 | T | A | -0.0428 | |
315 | A | A | 0.0000 | |
316 | T | A | -1.3403 | |
317 | I | A | 0.0000 | |
318 | E | A | -2.4542 | |
319 | G | A | -1.6483 | |
320 | R | A | -1.5385 | |
321 | K | A | -1.6431 | |
322 | V | A | 0.0000 | |
323 | M | A | 0.0000 | |
324 | L | A | 0.0000 | |
325 | Y | A | 0.0000 | |
326 | T | A | 0.0000 | |
327 | Q | A | 0.0000 | |
328 | K | A | 0.0000 | |
329 | V | A | 0.0000 | |
330 | R | A | -0.2025 | |
331 | H | A | -0.0346 | |
332 | F | A | 1.4712 | |
333 | L | A | 1.4367 | |
334 | E | A | -0.5342 | |
335 | V | A | 0.3094 | |
336 | D | A | -1.5065 | |
337 | E | A | -1.2011 | |
338 | P | A | -0.6816 | |
339 | N | A | 0.0000 | |
340 | A | A | 0.0000 | |
341 | L | A | 0.0000 | |
342 | H | A | 0.0000 | |
343 | L | A | 0.0000 | |
344 | W | A | 0.0000 | |
345 | V | A | 0.0000 | |
346 | T | A | 0.0000 | |
347 | D | A | 0.0000 | |
348 | N | A | -0.6024 | |
349 | N | A | -0.7276 | |
350 | R | A | 0.0000 | |
351 | T | A | 0.0000 | |
352 | F | A | -0.1656 | |
353 | H | A | -0.2952 | |
354 | L | A | 0.0000 | |
355 | G | A | 0.1855 | |
356 | P | A | 0.4135 | |
357 | F | A | 0.0000 | |
358 | S | A | 0.5933 | |
359 | V | A | 0.9887 | |
360 | D | A | -0.4343 | |
361 | S | A | -1.2096 | |
362 | A | A | -1.5451 | |
363 | E | A | -2.9529 | |
364 | N | A | -2.5887 | |
365 | K | A | -1.6238 | |
366 | T | A | 0.0000 | |
367 | F | A | 0.0754 | |
368 | A | A | 0.1027 | |
369 | N | A | 0.0000 | |
370 | T | A | 0.0000 | |
371 | L | A | 0.0000 | |
372 | L | A | 0.0478 | |
373 | Y | A | -0.5305 | |
374 | S | A | -1.5593 | |
375 | D | A | -2.9186 | |
376 | D | A | -3.0008 | |
377 | A | A | 0.0000 | |
378 | L | A | 0.0000 | |
379 | H | A | 0.0000 | |
380 | L | A | 0.0000 | |
381 | L | A | 0.0000 | |
382 | Q | A | 0.0000 | |
383 | A | A | 0.0000 | |
384 | K | A | -1.0769 | |
385 | G | A | 0.0000 | |
386 | D | A | -2.8409 | |
387 | H | A | -2.6930 | |
388 | E | A | -2.7404 | |
389 | S | A | -2.1455 | |
390 | T | A | 0.0000 | |
391 | A | A | -0.4460 | |
392 | V | A | 0.0000 | |
393 | S | A | 0.0000 | |
394 | L | A | 0.0000 | |
395 | A | A | 0.0000 | |
396 | R | A | -1.0657 | |
397 | L | A | 0.0000 | |
398 | T | A | -1.6012 | |
399 | E | A | -2.3981 | |
400 | E | A | 0.0000 | |
401 | L | A | 0.0000 | |
402 | N | A | -2.0753 | |
403 | T | A | -1.2907 | |
404 | I | A | 0.0000 | |
405 | N | A | -1.4717 | |
406 | S | A | -0.9141 | |
407 | V | A | 0.0000 | |
408 | L | A | 0.0000 | |
409 | S | A | -0.6844 | |
410 | T | A | -0.3880 | |
411 | W | A | 0.0000 | |
412 | V | A | 0.0891 | |
413 | Q | A | -0.7014 | |
414 | L | A | -0.4084 | |
415 | D | A | -0.5086 | |
416 | A | A | -0.7800 | |
417 | S | A | -0.9723 | |
418 | F | A | 0.0000 | |
419 | S | A | -1.3859 | |
420 | E | A | -2.0674 | |
421 | S | A | -1.4144 | |
422 | S | A | -0.9769 | |
423 | I | A | 0.0000 | |
424 | P | A | -0.3641 | |
425 | T | A | -0.1268 | |
426 | A | A | -0.2070 | |
427 | G | A | 0.0000 | |
428 | L | A | 0.0000 | |
429 | V | A | 0.0000 | |
430 | G | A | 0.0000 | |
431 | F | A | 0.0000 | |
432 | L | A | 0.0000 | |
433 | S | A | -1.3515 | |
434 | N | A | -2.0696 | |
435 | T | A | -1.7118 | |
436 | T | A | -1.0440 | |
437 | S | A | -0.9928 | |
438 | S | A | -1.1336 | |
439 | G | A | -1.9276 | |
440 | D | A | -2.2225 | |
441 | T | A | -1.4890 | |
442 | W | A | 0.0000 | |
443 | I | A | -0.7199 | |
444 | D | A | 0.0000 | |
445 | G | A | -0.6367 | |
446 | Y | A | 0.0000 | |
447 | R | A | -1.2870 | |
448 | S | A | 0.0000 | |
449 | M | A | -0.2556 | |
450 | N | A | -0.6137 | |
451 | A | A | 0.0000 | |
452 | T | A | -0.4024 | |
453 | V | A | 0.0000 | |
454 | T | A | -1.5403 | |
455 | K | A | -2.4353 | |
456 | A | A | -1.8068 | |
457 | A | A | -1.4949 | |
458 | K | A | -2.8139 | |
459 | V | A | -1.8909 | |
460 | E | A | -2.7634 | |
461 | N | A | -2.3817 | |
462 | G | A | 0.0000 | |
463 | F | A | 0.0000 | |
464 | K | A | -1.3973 | |
465 | F | A | 0.0000 | |
466 | T | A | -0.9860 | |
467 | G | A | -1.3408 | |
468 | P | A | -1.9277 | |
469 | G | A | -2.1561 | |
470 | S | A | 0.0000 | |
471 | R | A | -1.7195 | |
472 | A | A | 0.0000 | |
473 | T | A | 0.0000 | |
474 | W | A | 0.0000 | |
475 | P | A | 0.0000 | |
476 | V | A | 0.0000 | |
477 | N | A | 0.0000 | |
478 | S | A | -0.9929 | |
479 | R | A | -0.3716 | |
480 | W | A | -0.0714 | |
481 | D | A | 0.0000 | |
482 | I | A | 0.0000 | |
483 | K | A | -0.8344 | |
484 | Q | A | 0.0000 | |
485 | Y | A | 0.0000 | |
486 | G | A | -0.1243 | |
487 | F | A | 0.0000 | |
488 | V | A | 0.0000 | |
489 | D | A | 0.0000 | |
490 | Y | A | -0.5944 | |
491 | N | A | -1.3100 | |
492 | F | A | 0.0000 | |
493 | T | A | 0.0000 | |
494 | I | A | 0.0000 | |
495 | V | A | 0.0000 | |
496 | A | A | 0.0000 | |
497 | M | A | -0.5476 | |
498 | A | A | 0.0000 | |
499 | T | A | 0.0000 | |
500 | I | A | 0.0000 | |
501 | H | A | -1.7047 | |
502 | Q | A | -1.1043 | |
503 | V | A | 0.1675 | |
504 | P | A | 0.0000 | |
505 | S | A | -1.0979 | |
506 | E | A | -1.9391 | |
507 | S | A | -1.1764 | |
508 | T | A | 0.0000 | |
509 | P | A | -0.6606 | |
510 | L | A | 0.0000 | |
511 | L | A | 0.0000 | |
512 | G | A | 0.0000 | |
513 | A | A | 0.0000 | |
514 | S | A | 0.0000 | |
515 | L | A | 0.0000 | |
516 | R | A | -2.9072 | |
517 | G | A | -2.8047 | |
518 | N | A | -3.1524 | |
519 | K | A | -3.5612 | |
520 | R | A | -3.6896 | |
521 | T | A | -2.6901 | |
522 | K | A | -1.9796 | |
523 | L | A | 0.0000 | |
524 | I | A | 0.0000 | |
525 | G | A | 0.0000 | |
526 | L | A | 0.0000 | |
527 | S | A | 0.0000 | |
528 | Y | A | 0.0000 | |
529 | G | A | 0.0000 | |
530 | A | A | -0.6230 | |
531 | G | A | -0.6595 | |
532 | G | A | -1.0346 | |
533 | K | A | -1.4580 | |
534 | W | A | 0.0000 | |
535 | E | A | -0.4049 | |
536 | T | A | 0.0000 | |
537 | V | A | 0.0000 | |
538 | Y | A | 0.0000 | |
539 | D | A | -1.4261 | |
540 | G | A | -1.7993 | |
541 | T | A | -1.1204 | |
542 | K | A | -0.8215 | |
543 | T | A | -0.0637 | |
544 | V | A | 0.3590 | |
545 | Q | A | -0.6132 | |
546 | G | A | -0.8694 | |
547 | G | A | -0.6664 | |
548 | T | A | -1.2918 | |
549 | W | A | 0.0000 | |
550 | E | A | -2.9219 | |
551 | P | A | -2.2387 | |
552 | G | A | -2.6593 | |
553 | R | A | -3.5930 | |
554 | E | A | -3.2397 | |
555 | Y | A | 0.0000 | |
556 | Q | A | 0.0000 | |
557 | V | A | 0.0000 | |
558 | A | A | 0.0000 | |
559 | L | A | 0.0000 | |
560 | M | A | 0.0000 | |
561 | L | A | 0.0000 | |
562 | Q | A | -1.6582 | |
563 | D | A | -2.3286 | |
564 | G | A | 0.0000 | |
565 | N | A | -1.0628 | |
566 | K | A | -0.9949 | |
567 | G | A | 0.0000 | |
568 | F | A | -0.0784 | |
569 | V | A | 0.0000 | |
570 | Y | A | 1.0491 | |
571 | V | A | 0.0000 | |
572 | D | A | 0.0263 | |
573 | G | A | 0.0000 | |
574 | V | A | 2.0123 | |
575 | L | A | 1.1122 | |
576 | V | A | 0.0000 | |
577 | G | A | -0.5847 | |
578 | N | A | -1.2583 | |
579 | P | A | -0.4813 | |
580 | A | A | -0.1315 | |
581 | M | A | 0.4646 | |
582 | L | A | 0.0000 | |
583 | P | A | -0.9224 | |
584 | T | A | -1.4821 | |
585 | P | A | -1.3569 | |
586 | E | A | -2.4752 | |
587 | E | A | -2.4949 | |
588 | R | A | -1.2054 | |
589 | W | A | -0.4090 | |
590 | T | A | -0.9469 | |
591 | E | A | -1.2138 | |
592 | F | A | 0.0000 | |
593 | S | A | -1.2150 | |
594 | H | A | -0.7200 | |
595 | F | A | 0.0000 | |
596 | Y | A | 0.0000 | |
597 | F | A | 0.0000 | |
598 | G | A | 0.0000 | |
599 | G | A | 0.0000 | |
600 | D | A | -2.8874 | |
601 | E | A | -3.0151 | |
602 | G | A | -2.2426 | |
603 | D | A | 0.0000 | |
604 | S | A | -1.5392 | |
605 | G | A | -1.0165 | |
606 | S | A | 0.0000 | |
607 | D | A | -0.7450 | |
608 | A | A | 0.0000 | |
609 | T | A | -0.7172 | |
610 | L | A | 0.0000 | |
611 | T | A | -0.9001 | |
612 | D | A | -0.9797 | |
613 | V | A | 0.0000 | |
614 | F | A | 0.0000 | |
615 | L | A | 0.0000 | |
616 | Y | A | 0.0000 | |
617 | N | A | -0.7319 | |
618 | R | A | -1.1173 | |
619 | P | A | -0.7638 | |
620 | L | A | 0.0000 | |
621 | S | A | 0.0120 | |
622 | V | A | 1.0794 | |
623 | G | A | -0.2055 | |
624 | E | A | -0.3958 | |
625 | L | A | 0.0000 | |
626 | K | A | -0.9084 | |
627 | M | A | -0.7165 | |
628 | I | A | 0.0000 | |
629 | K | A | -2.0493 | |
630 | E | A | -2.4166 | |
631 | V | A | -0.9760 | |
632 | E | A | -2.6770 | |
633 | D | A | -2.5165 |
Automated mutations analysis
In the automated mutations mode, the server selects aggregation prone resides
and each selected residue is mutated to glutamic acid, lysine, aspartic acid and arginine.
The table below shows 2 best scored mutants for each mutated residue. Protein variants
are ordered according to the mutation effect they had on protein stability
(energetic effect) together with the difference in the average per-residue aggregation score
between the wild type and the mutant (in the table green values indicate a positive change,
grey are neutral, and orange/red mean destabilizing or more aggregation prone mutants).
Summary for all the mutants can be found in this
CSV file.
Mutant |
Energetic effect |
Score comparison |
|||
FR288A | -1.2511 | -0.0201 | View | CSV | PDB |
VR622A | -1.1 | -0.0188 | View | CSV | PDB |
LR333A | -1.3887 | -0.0149 | View | CSV | PDB |
VK622A | -0.8682 | -0.0188 | View | CSV | PDB |
VK574A | -0.5435 | -0.0182 | View | CSV | PDB |
VE574A | -0.441 | -0.0175 | View | CSV | PDB |
FR332A | -0.2936 | -0.0205 | View | CSV | PDB |
FD332A | 0.0071 | -0.0208 | View | CSV | PDB |
LK333A | 0.0249 | -0.0174 | View | CSV | PDB |
FK288A | 0.4251 | -0.0165 | View | CSV | PDB |
LR575A | 0.608 | -0.0112 | View | CSV | PDB |
LK575A | 0.8516 | -0.0087 | View | CSV | PDB |