Project name: GS linker_4

Chain sequence(s)	A: GGGGSGGGGSGGGGS; C: GGGGSGGGGSGGGGS; B: GGGGSGGGGSGGGGS; D: GGGGSGGGGSGGGGS
Selected Chain(s)	A,C,B,D
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:00:02)
[INFO]       Main:     Simulation completed successfully.                                          (00:00:03)

• Minimal score value: -1.7348
• Maximal score value: 0.0
• Average score: -1.3918
• Total score value: -83.5083

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	G	A	-0.9703
2	G	A	-1.3703
3	G	A	-1.4518
4	G	A	-1.6769
5	S	A	-1.3915
6	G	A	-1.3420
7	G	A	-1.4668
8	G	A	-1.4895
9	G	A	-1.3891
10	S	A	-1.4103
11	G	A	-1.4318
12	G	A	-1.5496
13	G	A	-1.6525
14	G	A	-1.7028
15	S	A	-1.2231
1	G	B	-1.2725
2	G	B	-1.2805
3	G	B	-1.4964
4	G	B	-1.3703
5	S	B	-1.4665
6	G	B	-1.3989
7	G	B	-1.4463
8	G	B	-1.4540
9	G	B	-1.3997
10	S	B	-1.5144
11	G	B	-1.6744
12	G	B	-1.7348
13	G	B	-1.6605
14	G	B	0.0000
15	S	B	-0.9099
1	G	C	-0.9799
2	G	C	-1.0375
3	G	C	-1.1470
4	G	C	-1.1316
5	S	C	-1.3214
6	G	C	-1.3911
7	G	C	-1.3728
8	G	C	-1.5009
9	G	C	-1.5358
10	S	C	-1.4504
11	G	C	-1.5716
12	G	C	-1.6292
13	G	C	-1.7127
14	G	C	-1.4278
15	S	C	-0.9126
1	G	D	-1.5042
2	G	D	-1.6462
3	G	D	-1.7109
4	G	D	-1.4842
5	S	D	-1.4536
6	G	D	-1.4585
7	G	D	-1.5761
8	G	D	-1.4706
9	G	D	-1.5671
10	S	D	-1.2718
11	G	D	-1.5504
12	G	D	-1.5665
13	G	D	-1.5430
14	G	D	-1.2451
15	S	D	-0.7404