Project name: 2FB4

Chain sequence(s)	H: EVQLVQSGGGVVQPGRSLRLSCSSSGFIFSSYAMYWVRQAPGKGLEWVAIIWDDGSDQHYADSVKGRFTISRNDSKNTLFLQMDSLRPEDTGVYFCARDGGHGFCSSASCFGPDYWGQGTPVTVSS; L: QSVLTQPPSASGTPGQRVTISCSGTSSNIGSSTVNWYQQLPGMAPKLLIYRDAMRPSGVPDRFSGSKSGASASLAIGGLQSEDETDYYCAAWDVSLNAYVFGTGTK
Selected Chain(s)	H,L
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:00:50)
[INFO]       Main:     Simulation completed successfully.                                          (00:00:51)

• Minimal score value: -3.0019
• Maximal score value: 1.875
• Average score: -0.5328
• Total score value: -123.6096

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	H	-1.9715
2	V	H	-0.4649
3	Q	H	-0.6387
4	L	H	0.0000
5	V	H	1.2547
6	Q	H	0.0000
7	S	H	-0.3476
8	G	H	-0.8375
9	G	H	-0.3296
11	G	H	0.4565
12	V	H	1.4102
13	V	H	-0.2684
14	Q	H	-1.7246
15	P	H	-2.1868
16	G	H	-1.9691
17	R	H	-2.7137
18	S	H	-1.8793
19	L	H	-1.1636
20	R	H	-1.5502
21	L	H	0.0000
22	S	H	-0.2031
23	C	H	0.0000
24	S	H	-0.2708
25	S	H	0.0000
26	S	H	-0.5177
27	G	H	-0.7111
28	F	H	0.5793
29	I	H	1.8750
30	F	H	0.0000
35	S	H	-0.5100
36	S	H	-0.0458
37	Y	H	0.0930
38	A	H	0.0000
39	M	H	0.0000
40	Y	H	0.0000
41	W	H	0.0000
42	V	H	0.0000
43	R	H	0.0000
44	Q	H	-0.7845
45	A	H	-1.1908
46	P	H	-0.9724
47	G	H	-1.4589
48	K	H	-2.2029
49	G	H	-1.3862
50	L	H	0.0000
51	E	H	-1.0353
52	W	H	0.0000
53	V	H	0.0000
54	A	H	0.0000
55	I	H	0.0000
56	I	H	0.0000
57	W	H	-1.0175
58	D	H	-1.3874
59	D	H	-2.4225
62	G	H	-1.7212
63	S	H	-1.4984
64	D	H	-1.8650
65	Q	H	-1.1918
66	H	H	-1.0482
67	Y	H	-1.0777
68	A	H	-1.3506
69	D	H	-2.3467
70	S	H	-1.8031
71	V	H	0.0000
72	K	H	-2.6753
74	G	H	-1.8293
75	R	H	-1.6609
76	F	H	0.0000
77	T	H	-0.9069
78	I	H	0.0000
79	S	H	-0.6957
80	R	H	-1.2751
81	N	H	-1.8148
82	D	H	-1.8933
83	S	H	-1.6272
84	K	H	-2.5068
85	N	H	-1.7437
86	T	H	0.0000
87	L	H	0.0000
88	F	H	-0.3061
89	L	H	0.0000
90	Q	H	-1.0443
91	M	H	0.0000
92	D	H	-2.0026
93	S	H	-1.7678
94	L	H	0.0000
95	R	H	-2.5708
96	P	H	-1.9210
97	E	H	-2.3353
98	D	H	0.0000
99	T	H	-0.6783
100	G	H	0.0000
101	V	H	0.1879
102	Y	H	0.0000
103	F	H	0.0000
104	C	H	0.0000
105	A	H	0.0000
106	R	H	0.0000
107	D	H	0.0000
108	G	H	0.0000
109	G	H	0.0000
110	H	H	-0.2390
111	G	H	0.3485
111A	F	H	1.2654
111B	C	H	1.0599
111C	S	H	0.4572
112C	S	H	0.4330
112B	A	H	0.5643
112A	S	H	0.0999
112	C	H	0.2403
113	F	H	0.4440
114	G	H	0.0000
115	P	H	0.0000
116	D	H	-0.0668
117	Y	H	0.4103
118	W	H	0.1709
119	G	H	0.0000
120	Q	H	-0.5667
121	G	H	0.0000
122	T	H	0.0000
123	P	H	-0.1189
124	V	H	0.0000
125	T	H	-0.0582
126	V	H	0.0000
127	S	H	-0.4543
128	S	H	-0.6463
1	Q	L	-1.0461
2	S	L	-0.3924
3	V	L	0.4242
4	L	L	0.0000
5	T	L	-0.2232
6	Q	L	0.0000
7	P	L	-0.5656
8	P	L	-0.8002
9	S	L	-1.0707
11	A	L	-1.0020
12	S	L	-1.2643
13	G	L	-1.5075
14	T	L	-1.1760
15	P	L	-1.4551
16	G	L	-1.8380
17	Q	L	-2.5645
18	R	L	-2.6457
19	V	L	-1.2089
20	T	L	-0.4935
21	I	L	0.0000
22	S	L	-0.3446
23	C	L	0.0000
24	S	L	-0.2728
25	G	L	-0.1722
26	T	L	-0.2596
27	S	L	-0.3231
28	S	L	0.3478
29	N	L	0.0000
30	I	L	0.0000
35	G	L	-0.7241
36	S	L	-0.1954
37	S	L	-0.1247
38	T	L	-0.4287
39	V	L	0.0000
40	N	L	0.0000
41	W	L	0.0000
42	Y	L	0.0000
43	Q	L	0.0000
44	Q	L	0.0000
45	L	L	-0.3895
46	P	L	-0.5287
47	G	L	-0.0106
48	M	L	0.5534
49	A	L	0.1598
50	P	L	0.0000
51	K	L	-0.3459
52	L	L	0.0000
53	L	L	0.0000
54	I	L	0.0000
55	Y	L	-0.1937
56	R	L	-0.7260
57	D	L	-0.7576
65	A	L	-0.3809
66	M	L	-0.0458
67	R	L	-1.1017
68	P	L	-0.5961
69	S	L	-0.6135
70	G	L	-0.9623
71	V	L	0.0000
72	P	L	-1.4229
74	D	L	-2.2578
75	R	L	-1.6248
76	F	L	0.0000
77	S	L	-0.7632
78	G	L	-0.4422
79	S	L	-0.6169
80	K	L	-1.0329
83	S	L	-0.7946
84	G	L	-0.8785
85	A	L	-0.6520
86	S	L	-0.6598
87	A	L	0.0000
88	S	L	-0.4235
89	L	L	0.0000
90	A	L	-0.5261
91	I	L	0.0000
92	G	L	-1.8687
93	G	L	-1.8218
94	L	L	-2.0721
95	Q	L	-2.0536
96	S	L	-2.0297
97	E	L	-2.6692
98	D	L	0.0000
99	E	L	-3.0019
100	T	L	0.0000
101	D	L	-1.3338
102	Y	L	0.0000
103	Y	L	0.0000
104	C	L	0.0000
105	A	L	0.0000
106	A	L	0.0000
107	W	L	0.6256
108	D	L	0.0000
109	V	L	1.5655
110	S	L	0.9454
113	L	L	1.1359
114	N	L	0.6359
115	A	L	0.2850
116	Y	L	0.0000
117	V	L	0.2904
118	F	L	0.0000
119	G	L	0.0000
120	T	L	-0.5783
121	G	L	0.0000
122	T	L	0.0000
123	K	L	-2.1828