Chain sequence(s) |
H: EVQLVESGGGLVQPGRSLRLSCAASGFTFDDYAMHWVRQAPGKGLEWVSAITWNSGHIDYADSVEGRFTISRDNAKNSLYLDMNSLRAEDTAVYYCAKVSYLSTASSLDYWGQGTLVTVSSASTKGPSVFPLAPSSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKI
L: DIQMTQSPSSLSASVGDRVTITCRASQGIRNYLAWYQQKPGKAPKLLIYAASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDVATYYCQRYNRAPYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input PDB |
Selected Chain(s) | H,L |
Distance of aggregation | 5 Å |
FoldX usage | Yes |
Dynamic mode | No |
Automated mutations | Yes |
Downloads | Download all the data |
Simulation log |
[INFO] Logger: Verbosity set to: 2 - [INFO] (00:00:00) [WARNING] runJob: Working directory already exists (possibly overwriting previous results -ow to prevent this behavior) (00:00:00) [INFO] runJob: Starting aggrescan3d job on: input.pdb with all chain(s) selected (00:00:00) [INFO] runJob: Creating pdb object from: input.pdb (00:00:00) [INFO] FoldX: Starting FoldX energy minimalization (00:00:00) [INFO] Analysis: Starting Aggrescan3D on folded.pdb (00:03:36) [INFO] Auto_mut: Residue number 5 from chain H and a score of 1.773 (valine) selected for automated muatation (00:03:39) [INFO] Auto_mut: Residue number 101 from chain H and a score of 1.541 (tyrosine) selected for automated muatation (00:03:39) [INFO] Auto_mut: Residue number 102 from chain H and a score of 1.497 (leucine) selected for automated muatation (00:03:39) [INFO] Auto_mut: Residue number 154 from chain L and a score of 1.484 (leucine) selected for automated muatation (00:03:39) [INFO] Auto_mut: Residue number 178 from chain H and a score of 1.400 (leucine) selected for automated muatation (00:03:39) [INFO] Auto_mut: Residue number 110 from chain L and a score of 1.145 (valine) selected for automated muatation (00:03:39) [INFO] Auto_mut: Mutating residue number 5 from chain H (valine) into glutamic acid (00:03:39) [INFO] Auto_mut: Mutating residue number 5 from chain H (valine) into aspartic acid (00:03:39) [INFO] Auto_mut: Mutating residue number 101 from chain H (tyrosine) into glutamic acid (00:03:39) [INFO] Auto_mut: Mutating residue number 5 from chain H (valine) into arginine (00:05:21) [INFO] Auto_mut: Mutating residue number 5 from chain H (valine) into lysine (00:05:23) [INFO] Auto_mut: Mutating residue number 101 from chain H (tyrosine) into lysine (00:05:32) [INFO] Auto_mut: Mutating residue number 101 from chain H (tyrosine) into aspartic acid (00:07:13) [INFO] Auto_mut: Mutating residue number 102 from chain H (leucine) into glutamic acid (00:07:27) [INFO] Auto_mut: Mutating residue number 102 from chain H (leucine) into aspartic acid (00:07:43) [INFO] Auto_mut: Mutating residue number 101 from chain H (tyrosine) into arginine (00:09:01) [INFO] Auto_mut: Mutating residue number 102 from chain H (leucine) into lysine (00:09:17) [INFO] Auto_mut: Mutating residue number 102 from chain H (leucine) into arginine (00:09:27) [INFO] Auto_mut: Mutating residue number 154 from chain L (leucine) into glutamic acid (00:10:52) [INFO] Auto_mut: Mutating residue number 154 from chain L (leucine) into aspartic acid (00:11:20) [INFO] Auto_mut: Mutating residue number 178 from chain H (leucine) into glutamic acid (00:11:21) [INFO] Auto_mut: Mutating residue number 154 from chain L (leucine) into lysine (00:12:49) [INFO] Auto_mut: Mutating residue number 178 from chain H (leucine) into lysine (00:13:03) [INFO] Auto_mut: Mutating residue number 154 from chain L (leucine) into arginine (00:13:09) [INFO] Auto_mut: Mutating residue number 178 from chain H (leucine) into aspartic acid (00:14:47) [INFO] Auto_mut: Mutating residue number 110 from chain L (valine) into glutamic acid (00:15:02) [INFO] Auto_mut: Mutating residue number 110 from chain L (valine) into aspartic acid (00:15:08) [INFO] Auto_mut: Mutating residue number 178 from chain H (leucine) into arginine (00:16:33) [INFO] Auto_mut: Mutating residue number 110 from chain L (valine) into lysine (00:16:40) [INFO] Auto_mut: Mutating residue number 110 from chain L (valine) into arginine (00:16:53) [INFO] Auto_mut: Effect of mutation residue number 5 from chain H (valine) into glutamic acid: Energy difference: -0.0334 kcal/mol, Difference in average score from the base case: -0.0099 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 5 from chain H (valine) into lysine: Energy difference: -0.1877 kcal/mol, Difference in average score from the base case: -0.0076 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 5 from chain H (valine) into aspartic acid: Energy difference: 0.2161 kcal/mol, Difference in average score from the base case: -0.0091 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 5 from chain H (valine) into arginine: Energy difference: -1.0110 kcal/mol, Difference in average score from the base case: -0.0081 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 101 from chain H (tyrosine) into glutamic acid: Energy difference: 0.5030 kcal/mol, Difference in average score from the base case: -0.0094 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 101 from chain H (tyrosine) into lysine: Energy difference: 0.0102 kcal/mol, Difference in average score from the base case: -0.0091 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 101 from chain H (tyrosine) into aspartic acid: Energy difference: -0.4267 kcal/mol, Difference in average score from the base case: -0.0094 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 101 from chain H (tyrosine) into arginine: Energy difference: -0.2973 kcal/mol, Difference in average score from the base case: -0.0095 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 102 from chain H (leucine) into glutamic acid: Energy difference: 0.3954 kcal/mol, Difference in average score from the base case: -0.0111 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 102 from chain H (leucine) into lysine: Energy difference: 0.3396 kcal/mol, Difference in average score from the base case: -0.0056 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 102 from chain H (leucine) into aspartic acid: Energy difference: 0.7538 kcal/mol, Difference in average score from the base case: -0.0123 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 102 from chain H (leucine) into arginine: Energy difference: -1.1506 kcal/mol, Difference in average score from the base case: -0.0084 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 154 from chain L (leucine) into glutamic acid: Energy difference: 0.2287 kcal/mol, Difference in average score from the base case: -0.0118 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 154 from chain L (leucine) into lysine: Energy difference: 0.1506 kcal/mol, Difference in average score from the base case: -0.0108 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 154 from chain L (leucine) into aspartic acid: Energy difference: 0.1969 kcal/mol, Difference in average score from the base case: -0.0119 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 154 from chain L (leucine) into arginine: Energy difference: 0.3515 kcal/mol, Difference in average score from the base case: -0.0121 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 178 from chain H (leucine) into glutamic acid: Energy difference: -0.0744 kcal/mol, Difference in average score from the base case: -0.0112 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 178 from chain H (leucine) into lysine: Energy difference: 0.1541 kcal/mol, Difference in average score from the base case: -0.0101 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 178 from chain H (leucine) into aspartic acid: Energy difference: 0.7791 kcal/mol, Difference in average score from the base case: -0.0114 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 178 from chain H (leucine) into arginine: Energy difference: 0.0920 kcal/mol, Difference in average score from the base case: -0.0110 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 110 from chain L (valine) into glutamic acid: Energy difference: 0.5490 kcal/mol, Difference in average score from the base case: -0.0118 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 110 from chain L (valine) into lysine: Energy difference: 0.6324 kcal/mol, Difference in average score from the base case: -0.0109 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 110 from chain L (valine) into aspartic acid: Energy difference: 0.7857 kcal/mol, Difference in average score from the base case: -0.0117 (00:18:36) [INFO] Auto_mut: Effect of mutation residue number 110 from chain L (valine) into arginine: Energy difference: 0.7427 kcal/mol, Difference in average score from the base case: -0.0114 (00:18:36) [INFO] Main: Simulation completed successfully. (00:18:42) |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
residue index | residue name | chain | Aggrescan3D score | mutation |
---|---|---|---|---|
residue index | residue name | chain | Aggrescan3D score | |
1 | E | H | -1.8405 | |
2 | V | H | -0.3707 | |
3 | Q | H | -0.8862 | |
4 | L | H | 0.0000 | |
5 | V | H | 1.7728 | |
6 | E | H | 0.0000 | |
7 | S | H | -0.2632 | |
8 | G | H | -0.3487 | |
9 | G | H | -0.2671 | |
10 | G | H | -0.0740 | |
11 | L | H | 0.2685 | |
12 | V | H | 0.0000 | |
13 | Q | H | -0.7896 | |
14 | P | H | -0.3325 | |
15 | G | H | -0.8523 | |
16 | R | H | -1.9565 | |
17 | S | H | -0.5093 | |
18 | L | H | -0.1566 | |
19 | R | H | -1.8639 | |
20 | L | H | 0.0000 | |
21 | S | H | -0.0076 | |
22 | C | H | 0.0000 | |
23 | A | H | 0.0362 | |
24 | A | H | 0.0000 | |
25 | S | H | -0.2988 | |
26 | G | H | -0.6476 | |
27 | F | H | 0.1971 | |
28 | T | H | -0.0165 | |
29 | F | H | 0.0000 | |
30 | D | H | -1.3954 | |
31 | D | H | -1.9399 | |
32 | Y | H | 0.1182 | |
33 | A | H | 0.0000 | |
34 | M | H | 0.0000 | |
35 | H | H | 0.0000 | |
36 | W | H | 0.0000 | |
37 | V | H | 0.0000 | |
38 | R | H | 0.0000 | |
39 | Q | H | -0.1414 | |
40 | A | H | -0.0634 | |
41 | P | H | -0.3390 | |
42 | G | H | -0.7438 | |
43 | K | H | -1.3586 | |
44 | G | H | -0.3186 | |
45 | L | H | 0.0000 | |
46 | E | H | -0.4232 | |
47 | W | H | 0.0000 | |
48 | V | H | 0.0000 | |
49 | S | H | 0.0000 | |
50 | A | H | 0.0000 | |
51 | I | H | 0.0000 | |
52 | T | H | 0.0950 | |
53 | W | H | 0.4483 | |
54 | N | H | -1.1786 | |
55 | S | H | -0.4735 | |
56 | G | H | -0.6475 | |
57 | H | H | -0.7604 | |
58 | I | H | 0.5566 | |
59 | D | H | -0.1000 | |
60 | Y | H | 0.1319 | |
61 | A | H | 0.0000 | |
62 | D | H | -1.8185 | |
63 | S | H | -0.4424 | |
64 | V | H | -0.1726 | |
65 | E | H | -1.8695 | |
66 | G | H | -0.8354 | |
67 | R | H | -0.3388 | |
68 | F | H | 0.0000 | |
69 | T | H | -0.0458 | |
70 | I | H | 0.0000 | |
71 | S | H | -0.1452 | |
72 | R | H | -0.3027 | |
73 | D | H | -0.5544 | |
74 | N | H | -0.4986 | |
75 | A | H | -0.3203 | |
76 | K | H | -1.7826 | |
77 | N | H | -0.8675 | |
78 | S | H | 0.0000 | |
79 | L | H | 0.0000 | |
80 | Y | H | 0.3365 | |
81 | L | H | 0.0000 | |
82 | D | H | -0.6722 | |
83 | M | H | 0.0000 | |
84 | N | H | -0.5819 | |
85 | S | H | -0.3300 | |
86 | L | H | 0.0000 | |
87 | R | H | -1.3384 | |
88 | A | H | -0.5208 | |
89 | E | H | -1.8091 | |
90 | D | H | 0.0000 | |
91 | T | H | -0.0118 | |
92 | A | H | 0.0000 | |
93 | V | H | 0.4092 | |
94 | Y | H | 0.0000 | |
95 | Y | H | 0.0000 | |
96 | C | H | 0.0000 | |
97 | A | H | 0.0000 | |
98 | K | H | 0.0000 | |
99 | V | H | 0.0000 | |
100 | S | H | 0.0000 | |
101 | Y | H | 1.5411 | |
102 | L | H | 1.4971 | |
103 | S | H | 0.1225 | |
104 | T | H | 0.0000 | |
105 | A | H | 0.0098 | |
106 | S | H | 0.0000 | |
107 | S | H | 0.0000 | |
108 | L | H | 0.0000 | |
109 | D | H | -0.2611 | |
110 | Y | H | 0.4600 | |
111 | W | H | 0.0000 | |
112 | G | H | 0.0000 | |
113 | Q | H | -1.1983 | |
114 | G | H | 0.0000 | |
115 | T | H | 0.0334 | |
116 | L | H | 0.2814 | |
117 | V | H | 0.0000 | |
118 | T | H | 0.0233 | |
119 | V | H | 0.0000 | |
120 | S | H | -0.0824 | |
121 | S | H | -0.2950 | |
122 | A | H | -0.0289 | |
123 | S | H | -0.2122 | |
124 | T | H | -0.2150 | |
125 | K | H | -0.7434 | |
126 | G | H | -0.2810 | |
127 | P | H | -0.0675 | |
128 | S | H | -0.0793 | |
129 | V | H | 0.0000 | |
130 | F | H | 0.1846 | |
131 | P | H | 0.0061 | |
132 | L | H | 0.0000 | |
133 | A | H | 0.0200 | |
134 | P | H | -0.1020 | |
135 | S | H | -0.2640 | |
136 | S | H | -0.2514 | |
142 | G | H | -0.4793 | |
143 | T | H | -0.1537 | |
144 | A | H | 0.0007 | |
145 | A | H | 0.0000 | |
146 | L | H | 0.0000 | |
147 | G | H | 0.0000 | |
148 | C | H | 0.0000 | |
149 | L | H | 0.0000 | |
150 | V | H | 0.0000 | |
151 | K | H | 0.0000 | |
152 | D | H | -0.5717 | |
153 | Y | H | 0.0000 | |
154 | F | H | 0.1910 | |
155 | P | H | 0.0000 | |
156 | E | H | -0.5199 | |
157 | P | H | -0.3110 | |
158 | V | H | 0.0000 | |
159 | T | H | -0.0357 | |
160 | V | H | 0.1599 | |
161 | S | H | -0.0660 | |
162 | W | H | 0.0000 | |
163 | N | H | -0.2461 | |
164 | S | H | -0.3374 | |
165 | G | H | -0.4491 | |
166 | A | H | 0.0281 | |
167 | L | H | 0.2580 | |
168 | T | H | -0.0618 | |
169 | S | H | -0.2546 | |
170 | G | H | -0.1305 | |
171 | V | H | 0.2991 | |
172 | H | H | -0.0443 | |
173 | T | H | -0.0525 | |
174 | F | H | 0.0000 | |
175 | P | H | -0.0990 | |
176 | A | H | 0.0502 | |
177 | V | H | 0.5408 | |
178 | L | H | 1.3999 | |
179 | Q | H | 0.0169 | |
180 | S | H | -0.2877 | |
181 | S | H | -0.3016 | |
182 | G | H | -0.2636 | |
183 | L | H | 0.2344 | |
184 | Y | H | 0.4066 | |
185 | S | H | 0.0161 | |
186 | L | H | 0.0000 | |
187 | S | H | 0.0000 | |
188 | S | H | 0.0000 | |
189 | V | H | 0.0000 | |
190 | V | H | 0.0000 | |
191 | T | H | -0.0156 | |
192 | V | H | 0.0000 | |
193 | P | H | -0.2937 | |
194 | S | H | -0.2982 | |
195 | S | H | -0.2657 | |
196 | S | H | -0.0218 | |
197 | L | H | 0.3312 | |
198 | G | H | -0.3851 | |
199 | T | H | -0.2859 | |
200 | Q | H | -0.6898 | |
201 | T | H | -0.1996 | |
202 | Y | H | 0.0000 | |
203 | I | H | 0.2101 | |
204 | C | H | 0.0000 | |
205 | N | H | -0.4942 | |
206 | V | H | 0.0000 | |
207 | N | H | -0.3717 | |
208 | H | H | 0.0000 | |
209 | K | H | -1.8843 | |
210 | P | H | -0.4054 | |
211 | S | H | -0.2894 | |
212 | N | H | -1.5117 | |
213 | T | H | -0.5746 | |
214 | K | H | -1.6292 | |
215 | V | H | -0.1223 | |
216 | D | H | -1.7786 | |
217 | K | H | -0.9410 | |
218 | K | H | -1.6270 | |
219 | I | H | 0.1265 | |
1 | D | L | -1.7500 | |
2 | I | L | -0.3248 | |
3 | Q | L | -1.1565 | |
4 | M | L | 0.0000 | |
5 | T | L | -0.0272 | |
6 | Q | L | 0.0000 | |
7 | S | L | -0.2420 | |
8 | P | L | -0.2013 | |
9 | S | L | -0.2709 | |
10 | S | L | -0.4018 | |
11 | L | L | 0.1682 | |
12 | S | L | -0.2020 | |
13 | A | L | -0.0357 | |
14 | S | L | -0.0932 | |
15 | V | L | 0.8977 | |
16 | G | L | -0.4180 | |
17 | D | L | -1.5498 | |
18 | R | L | -2.0766 | |
19 | V | L | 0.0000 | |
20 | T | L | -0.0519 | |
21 | I | L | 0.0000 | |
22 | T | L | -0.0340 | |
23 | C | L | 0.0000 | |
24 | R | L | -2.0532 | |
25 | A | L | 0.0000 | |
26 | S | L | -0.3179 | |
27 | Q | L | -0.6925 | |
28 | G | L | -0.5892 | |
29 | I | L | 0.0000 | |
30 | R | L | -1.9601 | |
31 | N | L | -0.7931 | |
32 | Y | L | 0.4410 | |
33 | L | L | 0.0000 | |
34 | A | L | 0.0000 | |
35 | W | L | 0.0000 | |
36 | Y | L | 0.0000 | |
37 | Q | L | 0.0000 | |
38 | Q | L | 0.0000 | |
39 | K | L | -0.4932 | |
40 | P | L | -0.2618 | |
41 | G | L | -0.7973 | |
42 | K | L | -1.7831 | |
43 | A | L | -0.2910 | |
44 | P | L | 0.0000 | |
45 | K | L | -1.1206 | |
46 | L | L | 0.0000 | |
47 | L | L | 0.0000 | |
48 | I | L | 0.0000 | |
49 | Y | L | 0.2012 | |
50 | A | L | 0.0059 | |
51 | A | L | 0.0000 | |
52 | S | L | -0.1893 | |
53 | T | L | 0.0059 | |
54 | L | L | 0.3835 | |
55 | Q | L | -0.1432 | |
56 | S | L | -0.3328 | |
57 | G | L | -0.5058 | |
58 | V | L | 0.0000 | |
59 | P | L | -0.1750 | |
60 | S | L | -0.2979 | |
61 | R | L | -0.3661 | |
62 | F | L | 0.0000 | |
63 | S | L | -0.1157 | |
64 | G | L | 0.0000 | |
65 | S | L | -0.2161 | |
66 | G | L | -0.2478 | |
67 | S | L | -0.2647 | |
68 | G | L | -0.4591 | |
69 | T | L | -0.4066 | |
70 | D | L | -1.9379 | |
71 | F | L | 0.0000 | |
72 | T | L | -0.0219 | |
73 | L | L | 0.0000 | |
74 | T | L | -0.0295 | |
75 | I | L | 0.0000 | |
76 | S | L | -0.3428 | |
77 | S | L | -0.3791 | |
78 | L | L | 0.0000 | |
79 | Q | L | -0.3457 | |
80 | P | L | -0.3141 | |
81 | E | L | -1.1371 | |
82 | D | L | 0.0000 | |
83 | V | L | 0.0000 | |
84 | A | L | 0.0000 | |
85 | T | L | 0.0000 | |
86 | Y | L | 0.0000 | |
87 | Y | L | 0.0000 | |
88 | C | L | 0.0000 | |
89 | Q | L | 0.0000 | |
90 | R | L | 0.0000 | |
91 | Y | L | 0.0000 | |
92 | N | L | -0.9337 | |
93 | R | L | -1.9599 | |
94 | A | L | -0.3505 | |
95 | P | L | -0.0567 | |
96 | Y | L | 0.0000 | |
97 | T | L | -0.0180 | |
98 | F | L | 0.0000 | |
99 | G | L | 0.0000 | |
100 | Q | L | -0.9578 | |
101 | G | L | 0.0000 | |
102 | T | L | 0.0000 | |
103 | K | L | -1.7245 | |
104 | V | L | 0.0000 | |
105 | E | L | -0.3217 | |
106 | I | L | 0.0000 | |
107 | K | L | -1.8011 | |
108 | R | L | -0.8488 | |
109 | T | L | 0.0468 | |
110 | V | L | 1.1448 | |
111 | A | L | 0.2467 | |
112 | A | L | 0.0082 | |
113 | P | L | 0.0000 | |
114 | S | L | -0.1710 | |
115 | V | L | 0.0000 | |
116 | F | L | 0.3053 | |
117 | I | L | 0.0000 | |
118 | F | L | 0.0000 | |
119 | P | L | -0.1141 | |
120 | P | L | -0.0500 | |
121 | S | L | -0.3617 | |
122 | D | L | -1.9659 | |
123 | E | L | -1.6109 | |
124 | Q | L | 0.0000 | |
125 | L | L | -0.2111 | |
126 | K | L | -1.8007 | |
127 | S | L | -0.5605 | |
128 | G | L | -0.3178 | |
129 | T | L | -0.1740 | |
130 | A | L | 0.0000 | |
131 | S | L | 0.0000 | |
132 | V | L | 0.0000 | |
133 | V | L | 0.0000 | |
134 | C | L | 0.0000 | |
135 | L | L | 0.0000 | |
136 | L | L | 0.0000 | |
137 | N | L | 0.0000 | |
138 | N | L | -0.5250 | |
139 | F | L | 0.0000 | |
140 | Y | L | 0.1515 | |
141 | P | L | -0.1913 | |
142 | R | L | -1.4286 | |
143 | E | L | -2.0161 | |
144 | A | L | -0.5965 | |
145 | K | L | -1.4410 | |
146 | V | L | -0.1072 | |
147 | Q | L | -0.2922 | |
148 | W | L | 0.0000 | |
149 | K | L | 0.0131 | |
150 | V | L | 0.0000 | |
151 | D | L | -1.4623 | |
152 | N | L | -1.4896 | |
153 | A | L | 0.0939 | |
154 | L | L | 1.4835 | |
155 | Q | L | 0.0165 | |
156 | S | L | -0.3427 | |
157 | G | L | -0.7415 | |
158 | N | L | -1.3735 | |
159 | S | L | -0.4552 | |
160 | Q | L | -1.1221 | |
161 | E | L | -1.7924 | |
162 | S | L | -0.2377 | |
163 | V | L | 0.4788 | |
164 | T | L | -0.2103 | |
165 | E | L | -1.5499 | |
166 | Q | L | 0.0000 | |
167 | D | L | -0.5601 | |
168 | S | L | -0.7465 | |
169 | K | L | -1.8025 | |
170 | D | L | -0.6681 | |
171 | S | L | 0.0000 | |
172 | T | L | 0.0000 | |
173 | Y | L | 0.0000 | |
174 | S | L | 0.0000 | |
175 | L | L | 0.0000 | |
176 | S | L | 0.0000 | |
177 | S | L | 0.0000 | |
178 | T | L | -0.0119 | |
179 | L | L | 0.0000 | |
180 | T | L | -0.0171 | |
181 | L | L | 0.0000 | |
182 | S | L | -0.2784 | |
183 | K | L | -0.7711 | |
184 | A | L | -0.1846 | |
185 | D | L | -0.5822 | |
186 | Y | L | 0.0000 | |
187 | E | L | -2.1320 | |
188 | K | L | -2.0711 | |
189 | H | L | -0.7903 | |
190 | K | L | -1.4682 | |
191 | V | L | 0.1427 | |
192 | Y | L | 0.0000 | |
193 | A | L | 0.0000 | |
194 | C | L | 0.0000 | |
195 | E | L | -0.4050 | |
196 | V | L | 0.0000 | |
197 | T | L | -0.0472 | |
198 | H | L | 0.0000 | |
199 | Q | L | -1.2333 | |
200 | G | L | -0.3641 | |
201 | L | L | 0.1473 | |
202 | S | L | -0.2110 | |
203 | S | L | -0.2827 | |
204 | P | L | -0.1187 | |
205 | V | L | 0.4582 | |
206 | T | L | -0.1414 | |
207 | K | L | -0.8031 | |
208 | S | L | -0.3164 | |
209 | F | L | 0.0361 | |
210 | N | L | -0.6554 | |
211 | R | L | -0.5576 | |
212 | G | L | -0.8635 | |
213 | E | L | -1.9053 |
Automated mutations analysis
In the automated mutations mode, the server selects aggregation prone resides
and each selected residue is mutated to glutamic acid, lysine, aspartic acid and arginine.
The table below shows 2 best scored mutants for each mutated residue. Protein variants
are ordered according to the mutation effect they had on protein stability
(energetic effect) together with the difference in the average per-residue aggregation score
between the wild type and the mutant (in the table green values indicate a positive change,
grey are neutral, and orange/red mean destabilizing or more aggregation prone mutants).
Summary for all the mutants can be found in this
CSV file.
Mutant |
Energetic effect |
Score comparison |
|||
LR102H | -1.1506 | -0.0084 | View | CSV | PDB |
VR5H | -1.011 | -0.0081 | View | CSV | PDB |
YD101H | -0.4267 | -0.0094 | View | CSV | PDB |
YR101H | -0.2973 | -0.0095 | View | CSV | PDB |
LE178H | -0.0744 | -0.0112 | View | CSV | PDB |
VE5H | -0.0334 | -0.0099 | View | CSV | PDB |
LR178H | 0.092 | -0.011 | View | CSV | PDB |
LK154L | 0.1506 | -0.0108 | View | CSV | PDB |
LD154L | 0.1969 | -0.0119 | View | CSV | PDB |
LE102H | 0.3954 | -0.0111 | View | CSV | PDB |
VE110L | 0.549 | -0.0118 | View | CSV | PDB |
VK110L | 0.6324 | -0.0109 | View | CSV | PDB |