Project name: CD8 VHH

Chain sequence(s)	A: EVQLVESGGGLVQPGGSLRLSCAASGFTFDEYAIGWFRQAPGKEREGVSCLRVSDGRTYYPDSVKGRFTISRDNSKNTVYLQMNSLRAEDTAVYYCASGSYYGCTVDDYDFWGQGTQVTVSSGGGGSGGGGSC
Selected Chain(s)	A
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with A chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:00:45)
[INFO]       Main:     Simulation completed successfully.                                          (00:00:46)

• Minimal score value: -3.3952
• Maximal score value: 1.5738
• Average score: -0.8308
• Total score value: -110.4922

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	A	-1.9797
2	V	A	-1.1102
3	Q	A	-0.8853
4	L	A	0.0000
5	V	A	1.2696
6	E	A	0.0000
7	S	A	-0.4860
8	G	A	-1.1552
9	G	A	-0.8324
10	G	A	-0.1183
11	L	A	0.8650
12	V	A	0.0000
13	Q	A	-1.4997
14	P	A	-1.6885
15	G	A	-1.4419
16	G	A	-1.0292
17	S	A	-1.3703
18	L	A	-1.0845
19	R	A	-2.1511
20	L	A	0.0000
21	S	A	-0.3626
22	C	A	0.0000
23	A	A	-0.0843
24	A	A	0.0000
25	S	A	-0.8847
26	G	A	-1.1148
27	F	A	-0.8726
28	T	A	-1.0984
29	F	A	0.0000
30	D	A	-2.9344
31	E	A	-2.4129
32	Y	A	-0.7077
33	A	A	0.0000
34	I	A	0.0000
35	G	A	0.0000
36	W	A	0.0000
37	F	A	0.0000
38	R	A	0.0000
39	Q	A	-1.8965
40	A	A	-1.8707
41	P	A	-1.3288
42	G	A	-1.8907
43	K	A	-3.2529
44	E	A	-3.3952
45	R	A	-2.3527
46	E	A	-2.0594
47	G	A	-0.5191
48	V	A	0.0000
49	S	A	0.0000
50	C	A	0.0000
51	L	A	-0.4633
52	R	A	-0.8448
53	V	A	-1.0383
54	S	A	-1.2505
55	D	A	-1.8004
56	G	A	-1.9115
57	R	A	-2.1915
58	T	A	-0.8380
59	Y	A	-0.1943
60	Y	A	-0.6133
61	P	A	-1.4347
62	D	A	-2.5051
63	S	A	-1.7619
64	V	A	0.0000
65	K	A	-2.5778
66	G	A	-1.7956
67	R	A	-1.5624
68	F	A	0.0000
69	T	A	-0.7808
70	I	A	0.0000
71	S	A	-0.5575
72	R	A	-1.4983
73	D	A	-2.2065
74	N	A	-2.8695
75	S	A	-2.0063
76	K	A	-2.6937
77	N	A	-2.3739
78	T	A	0.0000
79	V	A	0.0000
80	Y	A	-0.6261
81	L	A	0.0000
82	Q	A	-1.2637
83	M	A	0.0000
84	N	A	-1.5533
85	S	A	-1.2779
86	L	A	0.0000
87	R	A	-2.3669
88	A	A	-1.7481
89	E	A	-2.2517
90	D	A	0.0000
91	T	A	-0.8804
92	A	A	0.0000
93	V	A	-0.4290
94	Y	A	0.0000
95	Y	A	-0.1460
96	C	A	0.0000
97	A	A	0.0000
98	S	A	0.0000
99	G	A	0.0000
100	S	A	0.1795
101	Y	A	1.1379
102	Y	A	1.5738
103	G	A	0.7003
104	C	A	0.3232
105	T	A	-0.3315
106	V	A	-0.8816
107	D	A	-1.7845
108	D	A	-0.6830
109	Y	A	0.0000
110	D	A	-1.6077
111	F	A	-0.3533
112	W	A	0.0308
113	G	A	0.0775
114	Q	A	-0.7860
115	G	A	0.0000
116	T	A	-0.6513
117	Q	A	-1.0052
118	V	A	0.0000
119	T	A	-0.3576
120	V	A	0.0000
121	S	A	-0.9855
122	S	A	-1.1352
123	G	A	-1.0912
124	G	A	-1.1762
125	G	A	-1.2255
126	G	A	-1.2316
127	S	A	-1.0941
128	G	A	-1.2032
129	G	A	-1.2108
130	G	A	-1.0565
131	G	A	-0.7995
132	S	A	-0.2675
133	C	A	0.4524