Project name: obj1 [mutate: VA118C, VG116C, QH112C, WK110C, TW114C]

Chain sequence(s)	C: EVQLVESGGGLVQPGGSLRLSCAASDFTFRSYEMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARLRDGFNKGFDYWGQGTLVTVSS
Selected Chain(s)	C
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No
Mutated residues	VG116C,WK110C,QH112C,TW114C,VA118C
Energy difference between WT (input) and mutated protein (by FoldX)	16.5614 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with C chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       FoldX:    Building mutant model                                                       (00:00:25)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:01:05)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:01:30)
[INFO]       Main:     Simulation completed successfully.                                          (00:01:30)

• Minimal score value: -3.2343
• Maximal score value: 1.9788
• Average score: -0.6698
• Total score value: -80.3786

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	C	-2.0054
2	V	C	-1.2229
3	Q	C	-1.7611
4	L	C	0.0000
5	V	C	-0.0964
6	E	C	0.0000
7	S	C	-0.1624
8	G	C	-0.4823
9	G	C	0.2959
10	G	C	1.0026
11	L	C	1.4107
12	V	C	-0.0793
13	Q	C	-1.3502
14	P	C	-1.4913
15	G	C	-1.4131
16	G	C	-0.9714
17	S	C	-1.2360
18	L	C	-0.9141
19	R	C	-2.0731
20	L	C	0.0000
21	S	C	-0.4525
22	C	C	0.0000
23	A	C	-0.3418
24	A	C	0.0000
25	S	C	-0.2791
26	D	C	0.0000
27	F	C	1.5458
28	T	C	0.2525
29	F	C	0.0000
30	R	C	-2.0325
31	S	C	-0.8863
32	Y	C	-1.2160
33	E	C	-1.0755
34	M	C	0.0000
35	S	C	0.0000
36	W	C	0.0000
37	V	C	0.0000
38	R	C	0.0000
39	Q	C	-0.3476
40	A	C	-1.0175
41	P	C	-1.3235
42	G	C	-1.4330
43	K	C	-2.1226
44	G	C	-1.0118
45	L	C	0.4949
46	E	C	-0.3411
47	W	C	0.3563
48	V	C	0.0000
49	S	C	0.0000
50	A	C	0.0000
51	I	C	0.0000
52	S	C	-0.5831
53	G	C	-1.2447
54	S	C	-1.2286
55	G	C	-1.0817
56	G	C	-0.7345
57	S	C	-0.3025
58	T	C	0.1989
59	Y	C	0.6077
60	Y	C	-0.3562
61	A	C	-1.1390
62	D	C	-2.3443
63	S	C	-1.7150
64	V	C	0.0000
65	K	C	-2.3847
66	G	C	-1.6175
67	R	C	0.0000
68	F	C	0.0000
69	T	C	-0.6722
70	I	C	0.0000
71	S	C	-0.5624
72	R	C	-1.3626
73	D	C	-1.9805
74	N	C	-2.1901
75	S	C	-1.7905
76	K	C	-2.3163
77	N	C	-1.6492
78	T	C	0.0000
79	L	C	0.0000
80	Y	C	-0.6264
81	L	C	0.0000
82	Q	C	-1.2306
83	M	C	0.0000
84	N	C	-1.3292
85	S	C	-1.2273
86	L	C	0.0000
87	R	C	-2.4744
88	A	C	-1.8965
89	E	C	-2.3439
90	D	C	0.0000
91	T	C	-0.4747
92	A	C	0.0000
93	I	C	1.1584
94	Y	C	0.0000
95	Y	C	0.1798
96	C	C	0.0000
97	A	C	0.0000
98	R	C	0.0000
99	L	C	0.0000
100	R	C	-2.9677
101	D	C	-3.2343
102	G	C	-2.0119
103	F	C	-1.1504
104	N	C	-2.3475
105	K	C	-3.0491
106	G	C	-1.5987
107	F	C	-0.8138
108	D	C	-1.3269
109	Y	C	-1.1803
110	K	C	-1.7337	mutated: WK110C
111	G	C	0.0000
112	H	C	-1.2221	mutated: QH112C
113	G	C	-0.0035
114	W	C	1.0815	mutated: TW114C
115	L	C	1.9788
116	G	C	1.2327	mutated: VG116C
117	T	C	0.2900
118	A	C	0.0000	mutated: VA118C
119	S	C	-0.7694
120	S	C	-1.0574