Aggrescan3D: c6fa987f9f5c124

Project name: c6fa987f9f5c124

Status: done

Started: 2026-04-03 10:44:24

Settings

Chain sequence(s)	A: PNNQTYANYSAQGQPDLYPETLATLTLSFPDCEHGPLKNNLVCDSSAGYVERAQALISLFTLEELILNTQNSGPGVPRLGLPNYQVWNEALHGLDRANFATKGGQFEWATSFPMPILTTAALNRTLIHQIADIISTQARAFSNSGRYGLDVYAPNVNGFRSPLWGRGQETPGEDAFFLSSAYTYEYITGIQGGVDPEHHLKVAATVKHFAGYDLENWNNQSRLGFDAIITQQDLSEYYTPQFLAAARYAKSRSLMCAYNSVNGVPSCANSFFLQTLLRESWGFPEWGYVSSDCDAVYNVFNPHDYASNQSSSAAASSLRAGTDIDCGQTYPWHLNESFVAGEVSRGEIERSVTRLYANLVRLGYFDKKNQYRSSLGWKDVVKTDAWNISYEAAVEGIVLLKNDGTLPLSKKVRSIALIGPWANATTQMQGNYYGPAPYLISPLEAAKKAGYHHVNFELGTEIAGNSTTGFAKAIAAAKKSDAIIYLGGIDNTIEQEGADRTDIAWPGNQLDLIKQLSEVGKPLVVLQMGGGQVDSSSLKSNKKVNSLVWGGYPGQSGGVALFDILSGKRAPAGRLVTTQYPAEYVHQFPQNDMNLRPDGKSNPGQTYIWYTGKPVYEFGSGLFYTTFKETLALKFNTSSILSAPHPGYTYSEQIPVFTFEANIKNSGKTESPYTAMLFVRTSSNAGPAPYPNKWLVGFDRLADIKPGHSSKLSIPIPVSALARVDSSHGNRIVYPGKYELALNTDESVKLEFELVGEEVTIENWPLEE input PDB
Selected Chain(s)	A
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	Yes
Downloads	Download all the data

Simulation log

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[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with A chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:11:27)
[INFO]       Auto_mut: Residue number 337 from chain A and a score of 1.224 (valine) selected for  
                       automated muatation                                                         (00:11:37)
[INFO]       Auto_mut: Residue number 227 from chain A and a score of 1.197 (isoleucine) selected  
                       for automated muatation                                                     (00:11:37)
[INFO]       Auto_mut: Residue number 194 from chain A and a score of 1.153 (valine) selected for  
                       automated muatation                                                         (00:11:37)
[INFO]       Auto_mut: Residue number 435 from chain A and a score of 0.692 (tyrosine) selected    
                       for automated muatation                                                     (00:11:37)
[INFO]       Auto_mut: Residue number 618 from chain A and a score of 0.676 (leucine) selected for 
                       automated muatation                                                         (00:11:37)
[INFO]       Auto_mut: Residue number 6 from chain A and a score of 0.615 (tyrosine) selected for  
                       automated muatation                                                         (00:11:37)
[INFO]       Auto_mut: Mutating residue number 337 from chain A (valine) into glutamic acid        (00:11:37)
[INFO]       Auto_mut: Mutating residue number 337 from chain A (valine) into aspartic acid        (00:11:37)
[INFO]       Auto_mut: Mutating residue number 227 from chain A (isoleucine) into glutamic acid    (00:11:37)
[INFO]       Auto_mut: Mutating residue number 337 from chain A (valine) into arginine             (00:18:31)
[INFO]       Auto_mut: Mutating residue number 337 from chain A (valine) into lysine               (00:18:37)
[INFO]       Auto_mut: Mutating residue number 227 from chain A (isoleucine) into lysine           (00:18:47)
[INFO]       Auto_mut: Mutating residue number 227 from chain A (isoleucine) into aspartic acid    (00:24:37)
[INFO]       Auto_mut: Mutating residue number 194 from chain A (valine) into glutamic acid        (00:24:39)
[INFO]       Auto_mut: Mutating residue number 194 from chain A (valine) into aspartic acid        (00:24:53)
[INFO]       Auto_mut: Mutating residue number 227 from chain A (isoleucine) into arginine         (00:29:47)
[INFO]       Auto_mut: Mutating residue number 194 from chain A (valine) into lysine               (00:30:37)
[INFO]       Auto_mut: Mutating residue number 194 from chain A (valine) into arginine             (00:30:50)
[INFO]       Auto_mut: Mutating residue number 435 from chain A (tyrosine) into glutamic acid      (00:35:24)
[INFO]       Auto_mut: Mutating residue number 435 from chain A (tyrosine) into aspartic acid      (00:36:23)
[INFO]       Auto_mut: Mutating residue number 618 from chain A (leucine) into glutamic acid       (00:37:00)
[INFO]       Auto_mut: Mutating residue number 435 from chain A (tyrosine) into lysine             (00:41:17)
[INFO]       Auto_mut: Mutating residue number 435 from chain A (tyrosine) into arginine           (00:42:16)
[INFO]       Auto_mut: Mutating residue number 618 from chain A (leucine) into lysine              (00:43:07)
[INFO]       Auto_mut: Mutating residue number 618 from chain A (leucine) into aspartic acid       (00:47:36)
[INFO]       Auto_mut: Mutating residue number 6 from chain A (tyrosine) into glutamic acid        (00:48:08)
[INFO]       Auto_mut: Mutating residue number 6 from chain A (tyrosine) into aspartic acid        (00:48:59)
[INFO]       Auto_mut: Mutating residue number 618 from chain A (leucine) into arginine            (00:52:28)
[INFO]       Auto_mut: Mutating residue number 6 from chain A (tyrosine) into lysine               (00:52:57)
[INFO]       Auto_mut: Mutating residue number 6 from chain A (tyrosine) into arginine             (00:53:29)
[INFO]       Auto_mut: Effect of mutation residue number 337 from chain A (valine) into glutamic   
                       acid: Energy difference: 0.0108 kcal/mol, Difference in average score from  
                       the base case: -0.0098                                                      (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 337 from chain A (valine) into lysine:    
                       Energy difference: -0.3939 kcal/mol, Difference in average score from the   
                       base case: -0.0061                                                          (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 337 from chain A (valine) into aspartic   
                       acid: Energy difference: 0.3358 kcal/mol, Difference in average score from  
                       the base case: -0.0106                                                      (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 337 from chain A (valine) into arginine:  
                       Energy difference: -0.6735 kcal/mol, Difference in average score from the   
                       base case: -0.0066                                                          (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 227 from chain A (isoleucine) into        
                       glutamic acid: Energy difference: 0.9042 kcal/mol, Difference in average    
                       score from the base case: -0.0101                                           (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 227 from chain A (isoleucine) into        
                       lysine: Energy difference: 0.3074 kcal/mol, Difference in average score     
                       from the base case: -0.0102                                                 (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 227 from chain A (isoleucine) into        
                       aspartic acid: Energy difference: 0.6253 kcal/mol, Difference in average    
                       score from the base case: -0.0095                                           (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 227 from chain A (isoleucine) into        
                       arginine: Energy difference: -0.3230 kcal/mol, Difference in average score  
                       from the base case: -0.0061                                                 (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 194 from chain A (valine) into glutamic   
                       acid: Energy difference: -0.0473 kcal/mol, Difference in average score from 
                       the base case: -0.0091                                                      (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 194 from chain A (valine) into lysine:    
                       Energy difference: -0.0880 kcal/mol, Difference in average score from the   
                       base case: -0.0061                                                          (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 194 from chain A (valine) into aspartic   
                       acid: Energy difference: 0.4262 kcal/mol, Difference in average score from  
                       the base case: -0.0085                                                      (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 194 from chain A (valine) into arginine:  
                       Energy difference: -0.7149 kcal/mol, Difference in average score from the   
                       base case: -0.0047                                                          (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 435 from chain A (tyrosine) into glutamic 
                       acid: Energy difference: 2.9812 kcal/mol, Difference in average score from  
                       the base case: -0.0057                                                      (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 435 from chain A (tyrosine) into lysine:  
                       Energy difference: 1.7770 kcal/mol, Difference in average score from the    
                       base case: -0.0066                                                          (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 435 from chain A (tyrosine) into aspartic 
                       acid: Energy difference: 2.8670 kcal/mol, Difference in average score from  
                       the base case: -0.0057                                                      (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 435 from chain A (tyrosine) into          
                       arginine: Energy difference: 1.7569 kcal/mol, Difference in average score   
                       from the base case: -0.0042                                                 (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 618 from chain A (leucine) into glutamic  
                       acid: Energy difference: 1.2334 kcal/mol, Difference in average score from  
                       the base case: -0.0036                                                      (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 618 from chain A (leucine) into lysine:   
                       Energy difference: -0.2124 kcal/mol, Difference in average score from the   
                       base case: -0.0011                                                          (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 618 from chain A (leucine) into aspartic  
                       acid: Energy difference: 1.0885 kcal/mol, Difference in average score from  
                       the base case: -0.0035                                                      (00:57:55)
[INFO]       Auto_mut: Effect of mutation residue number 618 from chain A (leucine) into arginine: 
                       Energy difference: 0.1514 kcal/mol, Difference in average score from the    
                       base case: -0.0054                                                          (00:57:56)
[INFO]       Auto_mut: Effect of mutation residue number 6 from chain A (tyrosine) into glutamic   
                       acid: Energy difference: 0.1477 kcal/mol, Difference in average score from  
                       the base case: -0.0069                                                      (00:57:56)
[INFO]       Auto_mut: Effect of mutation residue number 6 from chain A (tyrosine) into lysine:    
                       Energy difference: 0.0756 kcal/mol, Difference in average score from the    
                       base case: -0.0084                                                          (00:57:56)
[INFO]       Auto_mut: Effect of mutation residue number 6 from chain A (tyrosine) into aspartic   
                       acid: Energy difference: 0.1426 kcal/mol, Difference in average score from  
                       the base case: -0.0067                                                      (00:57:56)
[INFO]       Auto_mut: Effect of mutation residue number 6 from chain A (tyrosine) into arginine:  
                       Energy difference: 0.1533 kcal/mol, Difference in average score from the    
                       base case: -0.0040                                                          (00:57:56)
[INFO]       Main:     Simulation completed successfully.                                          (00:58:10)

Note Your prediction results will be deleted after 360 days.

Drag cursor over the plot to display residue labels.

Show buried residues

Minimal score value: -3.5847
Maximal score value: 1.2239
Average score: -0.5353
Total score value: -407.8642

Download the table (csv)

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
residue index	residue name	chain	Aggrescan3D score	mutation
1	P	A	-0.6785
2	N	A	-1.1034
3	N	A	-1.3059
4	Q	A	-1.3822
5	T	A	-0.3668
6	Y	A	0.6145
7	A	A	-0.5270
8	N	A	-1.2479
9	Y	A	0.0000
10	S	A	-1.1730
11	A	A	-1.6163
12	Q	A	-1.4736
13	G	A	0.0000
14	Q	A	-0.9767
15	P	A	-0.8836
16	D	A	-1.0843
17	L	A	-0.3509
18	Y	A	-0.3371
19	P	A	-0.4866
20	E	A	-0.8655
21	T	A	0.0000
22	L	A	0.3681
23	A	A	-0.0814
24	T	A	-0.2150
25	L	A	-0.1548
26	T	A	-0.2824
27	L	A	0.0000
28	S	A	-0.4293
29	F	A	-0.5214
30	P	A	0.0000
31	D	A	-3.0470
32	C	A	-3.0631
33	E	A	-3.5847
34	H	A	-2.9540
35	G	A	-2.5263
36	P	A	-2.0176
37	L	A	0.0000
38	K	A	-3.2209
39	N	A	-2.3756
40	N	A	-1.0109
41	L	A	-0.7543
42	V	A	0.0000
43	C	A	-1.5114
44	D	A	-1.5080
45	S	A	-0.9738
46	S	A	-0.4915
47	A	A	-0.6537
48	G	A	-0.8078
49	Y	A	-0.5424
50	V	A	-0.8207
51	E	A	-1.9847
52	R	A	0.0000
53	A	A	0.0000
54	Q	A	-1.8280
55	A	A	-0.6877
56	L	A	0.0000
57	I	A	0.0000
58	S	A	-0.2647
59	L	A	0.3140
60	F	A	0.0000
61	T	A	-0.4848
62	L	A	-0.8475
63	E	A	-1.7414
64	E	A	-0.8090
65	L	A	0.0000
66	I	A	0.0000
67	L	A	0.0650
68	N	A	0.0000
69	T	A	0.0000
70	Q	A	0.0000
71	N	A	0.0000
72	S	A	0.0000
73	G	A	0.0000
74	P	A	-0.2069
75	G	A	0.0000
76	V	A	0.0000
77	P	A	-1.2483
78	R	A	-1.9101
79	L	A	0.0000
80	G	A	-1.4749
81	L	A	0.0000
82	P	A	-0.6857
83	N	A	-0.4056
84	Y	A	0.0000
85	Q	A	0.0000
86	V	A	0.0000
87	W	A	0.0000
88	N	A	0.0000
89	E	A	0.0000
90	A	A	0.0000
91	L	A	0.0000
92	H	A	0.0000
93	G	A	0.0000
94	L	A	0.0000
95	D	A	-0.3884
96	R	A	-0.7261
97	A	A	0.0000
98	N	A	-0.6893
99	F	A	-0.7030
100	A	A	-1.1526
101	T	A	-1.3052
102	K	A	-2.3819
103	G	A	-1.9568
104	G	A	-1.8173
105	Q	A	-1.9842
106	F	A	0.0000
107	E	A	-1.3652
108	W	A	-0.2048
109	A	A	0.0000
110	T	A	0.0000
111	S	A	0.0000
112	F	A	0.0000
113	P	A	0.0000
114	M	A	0.0000
115	P	A	0.0000
116	I	A	0.0000
117	L	A	0.0000
118	T	A	0.0000
119	T	A	0.0000
120	A	A	0.0000
121	A	A	0.0000
122	L	A	0.0000
123	N	A	-0.7084
124	R	A	-0.8537
125	T	A	-0.6567
126	L	A	0.0000
127	I	A	0.0000
128	H	A	-0.9268
129	Q	A	-0.8594
130	I	A	0.0000
131	A	A	0.0000
132	D	A	-0.9133
133	I	A	0.0000
134	I	A	0.0000
135	S	A	0.0000
136	T	A	-0.1874
137	Q	A	0.0000
138	A	A	0.0000
139	R	A	0.0000
140	A	A	0.0000
141	F	A	0.0000
142	S	A	0.0000
143	N	A	-0.5355
144	S	A	-0.5473
145	G	A	0.0000
146	R	A	-0.8051
147	Y	A	0.0000
148	G	A	0.0000
149	L	A	0.0000
150	D	A	0.0000
151	V	A	0.0000
152	Y	A	0.0000
153	A	A	0.0000
154	P	A	0.0000
155	N	A	0.0000
156	V	A	0.0000
157	N	A	0.0000
158	G	A	0.0000
159	F	A	0.0000
160	R	A	0.0000
161	S	A	0.0000
162	P	A	0.0000
163	L	A	0.0000
164	W	A	0.0000
165	G	A	0.0000
166	R	A	0.0000
167	G	A	0.0000
168	Q	A	0.0000
169	E	A	0.0000
170	T	A	0.0000
171	P	A	0.0000
172	G	A	0.0000
173	E	A	0.0000
174	D	A	0.0000
175	A	A	0.0000
176	F	A	0.3718
177	F	A	0.3872
178	L	A	0.0000
179	S	A	0.0000
180	S	A	0.2686
181	A	A	0.0718
182	Y	A	0.0000
183	T	A	0.0000
184	Y	A	0.4017
185	E	A	-0.4846
186	Y	A	0.0000
187	I	A	0.0000
188	T	A	0.0000
189	G	A	0.0000
190	I	A	0.0000
191	Q	A	0.0000
192	G	A	-0.5356
193	G	A	-0.0165
194	V	A	1.1529
195	D	A	-0.3660
196	P	A	-1.0591
197	E	A	-2.2363
198	H	A	-2.2223
199	L	A	0.0000
200	K	A	0.0000
201	V	A	0.0000
202	A	A	0.0000
203	A	A	0.0000
204	T	A	0.0000
205	V	A	0.0000
206	K	A	0.0000
207	H	A	0.0000
208	F	A	0.0000
209	A	A	0.0000
210	G	A	0.0000
211	Y	A	0.0000
212	D	A	0.0000
213	L	A	0.0000
214	E	A	0.0000
215	N	A	-1.4520
216	W	A	-1.2796
217	N	A	-2.1529
218	N	A	-2.3360
219	Q	A	-1.8822
220	S	A	0.0000
221	R	A	0.0000
222	L	A	0.0000
223	G	A	-0.9440
224	F	A	-0.3115
225	D	A	-0.5869
226	A	A	0.0000
227	I	A	1.1968
228	I	A	0.0000
229	T	A	-0.2911
230	Q	A	-0.7903
231	Q	A	-0.9777
232	D	A	0.0000
233	L	A	0.0000
234	S	A	0.0000
235	E	A	-0.3952
236	Y	A	0.0000
237	Y	A	0.0000
238	T	A	0.0000
239	P	A	0.0000
240	Q	A	0.0000
241	F	A	0.0000
242	L	A	0.1255
243	A	A	0.0000
244	A	A	0.0000
245	A	A	0.0000
246	R	A	0.0000
247	Y	A	0.4696
248	A	A	0.0000
249	K	A	0.1094
250	S	A	0.0000
251	R	A	0.0000
252	S	A	0.0000
253	L	A	0.0000
254	M	A	0.0000
255	C	A	0.0000
256	A	A	0.0000
257	Y	A	0.0000
258	N	A	0.0000
259	S	A	0.0000
260	V	A	0.0000
261	N	A	-0.5288
262	G	A	-0.3018
263	V	A	-0.0059
264	P	A	0.0000
265	S	A	0.0000
266	C	A	0.0000
267	A	A	0.0000
268	N	A	0.0000
269	S	A	-1.1337
270	F	A	0.0000
271	F	A	0.0000
272	L	A	0.0000
273	Q	A	-1.4380
274	T	A	-1.4899
275	L	A	0.0000
276	L	A	0.0000
277	R	A	-1.8735
278	E	A	-2.2696
279	S	A	-1.4163
280	W	A	0.0000
281	G	A	-1.2599
282	F	A	0.0000
283	P	A	0.0000
284	E	A	-1.8731
285	W	A	-0.9535
286	G	A	0.0000
287	Y	A	0.0000
288	V	A	0.0000
289	S	A	0.0000
290	S	A	0.0000
291	D	A	0.0000
292	C	A	0.0000
293	D	A	0.0000
294	A	A	0.0000
295	V	A	0.0000
296	Y	A	0.0000
297	N	A	0.0000
298	V	A	0.0000
299	F	A	-0.6518
300	N	A	-0.9805
301	P	A	-0.8857
302	H	A	-0.8722
303	D	A	-1.5333
304	Y	A	-0.0013
305	A	A	-0.4815
306	S	A	-0.8877
307	N	A	-1.1469
308	Q	A	-0.7697
309	S	A	-0.6214
310	S	A	-0.5300
311	A	A	0.0000
312	A	A	0.0000
313	A	A	0.0000
314	S	A	-0.6191
315	S	A	0.0000
316	L	A	0.0000
317	R	A	-2.0744
318	A	A	0.0000
319	G	A	-0.9475
320	T	A	0.0000
321	D	A	0.0000
322	I	A	0.0000
323	D	A	0.0000
324	C	A	0.0000
325	G	A	0.0000
326	Q	A	0.0000
327	T	A	0.0000
328	Y	A	0.0000
329	P	A	0.0000
330	W	A	0.0548
331	H	A	-0.3529
332	L	A	0.0000
333	N	A	-0.8386
334	E	A	-0.7481
335	S	A	0.0000
336	F	A	0.3806
337	V	A	1.2239
338	A	A	0.3182
339	G	A	-0.2184
340	E	A	-0.6885
341	V	A	0.0000
342	S	A	-0.7914
343	R	A	-1.2379
344	G	A	-1.3996
345	E	A	-1.9997
346	I	A	0.0000
347	E	A	-1.8612
348	R	A	-2.5399
349	S	A	0.0000
350	V	A	0.0000
351	T	A	0.0000
352	R	A	-1.1635
353	L	A	0.0000
354	Y	A	0.0000
355	A	A	0.0000
356	N	A	0.0000
357	L	A	0.0000
358	V	A	0.0000
359	R	A	-0.7999
360	L	A	0.0000
361	G	A	0.0000
362	Y	A	0.0000
363	F	A	0.0000
364	D	A	-2.2342
365	K	A	-3.2379
366	K	A	-2.9708
367	N	A	0.0000
368	Q	A	-2.0607
369	Y	A	-1.2234
370	R	A	-1.5633
371	S	A	-1.1141
372	L	A	-0.5567
373	G	A	-0.7789
374	W	A	-1.2858
375	K	A	-2.1259
376	D	A	-1.4579
377	V	A	0.0000
378	V	A	-0.8058
379	K	A	-1.7126
380	T	A	0.0000
381	D	A	0.0000
382	A	A	-0.1037
383	W	A	0.3772
384	N	A	-0.2210
385	I	A	0.1578
386	S	A	0.0000
387	Y	A	0.0542
388	E	A	-0.2714
389	A	A	0.0000
390	A	A	0.0000
391	V	A	0.0000
392	E	A	-0.4025
393	G	A	0.0000
394	I	A	0.0000
395	V	A	0.0000
396	L	A	0.0000
397	L	A	0.0000
398	K	A	-0.9941
399	N	A	-1.5105
400	D	A	-2.4966
401	G	A	-1.5367
402	T	A	-1.0332
403	L	A	0.0000
404	P	A	-0.8761
405	L	A	0.0000
406	S	A	-1.9570
407	K	A	-2.9968
408	K	A	-3.1429
409	V	A	0.0000
410	R	A	-3.0052
411	S	A	-1.8862
412	I	A	0.0000
413	A	A	0.0000
414	L	A	0.0000
415	I	A	0.0000
416	G	A	0.0000
417	P	A	0.0000
418	W	A	0.0000
419	A	A	0.0000
420	N	A	-0.8953
421	A	A	0.0000
422	T	A	-0.2324
423	T	A	-0.1644
424	Q	A	-0.4961
425	M	A	0.0000
426	Q	A	0.0000
427	G	A	0.0000
428	N	A	-0.0974
429	Y	A	0.2510
430	Y	A	0.4075
431	G	A	-0.0691
432	P	A	0.0155
433	A	A	0.2019
434	P	A	0.2641
435	Y	A	0.6915
436	L	A	0.5359
437	I	A	0.2879
438	S	A	0.0000
439	P	A	0.0000
440	L	A	-1.0517
441	E	A	-1.9348
442	A	A	0.0000
443	A	A	0.0000
444	K	A	-3.0792
445	K	A	-2.9328
446	A	A	-1.7259
447	G	A	-1.9955
448	Y	A	-1.9525
449	H	A	-1.9479
450	V	A	-0.8601
451	N	A	-0.1323
452	F	A	0.5003
453	E	A	0.0000
454	L	A	0.1085
455	G	A	0.0000
456	T	A	0.0000
457	E	A	-2.0288
458	I	A	0.0000
459	A	A	-0.9976
460	G	A	-1.5532
461	N	A	-1.8523
462	S	A	-1.3018
463	T	A	-0.8964
464	T	A	-0.5929
465	G	A	-0.6689
466	F	A	0.0000
467	A	A	-0.6864
468	K	A	-1.5441
469	A	A	0.0000
470	I	A	-1.0110
471	A	A	-1.3780
472	A	A	0.0000
473	A	A	0.0000
474	K	A	-2.7904
475	K	A	-2.6177
476	S	A	0.0000
477	D	A	-1.9580
478	A	A	0.0000
479	I	A	0.0000
480	I	A	0.0000
481	Y	A	0.0000
482	L	A	0.0000
483	G	A	0.0000
484	G	A	0.0000
485	I	A	0.0000
486	D	A	0.0000
487	N	A	-0.4987
488	T	A	-0.1662
489	I	A	-0.0186
490	E	A	0.0000
491	Q	A	-0.7658
492	E	A	-0.7624
493	G	A	0.0000
494	A	A	-0.6737
495	D	A	-0.7812
496	R	A	0.0000
497	T	A	-0.8625
498	D	A	-1.4674
499	I	A	0.0000
500	A	A	-0.6218
501	W	A	0.0000
502	P	A	-0.6261
503	G	A	-1.2280
504	N	A	-1.2370
505	Q	A	0.0000
506	L	A	-0.6822
507	D	A	-1.4600
508	L	A	0.0000
509	I	A	0.0000
510	K	A	-2.5592
511	Q	A	-1.7801
512	L	A	0.0000
513	S	A	0.0000
514	E	A	-2.8162
515	V	A	0.0000
516	G	A	-1.8755
517	K	A	-1.8994
518	P	A	-1.0167
519	L	A	0.0000
520	V	A	0.0000
521	V	A	0.0000
522	L	A	0.0000
523	Q	A	0.0000
524	M	A	0.0000
525	G	A	0.0000
526	G	A	0.0000
527	G	A	0.0000
528	Q	A	0.0000
529	V	A	0.0000
530	D	A	-0.7192
531	S	A	0.0000
532	S	A	-0.7416
533	S	A	-1.0124
534	L	A	0.0000
535	K	A	-2.0100
536	S	A	-1.6157
537	N	A	-2.3287
538	K	A	-3.1639
539	K	A	-3.5392
540	V	A	0.0000
541	N	A	-2.1047
542	S	A	0.0000
543	L	A	0.0000
544	V	A	0.0000
545	W	A	0.0000
546	G	A	0.0000
547	G	A	0.0000
548	Y	A	0.0000
549	P	A	0.0000
550	G	A	0.0000
551	Q	A	0.0000
552	S	A	0.0000
553	G	A	0.0000
554	G	A	0.0000
555	V	A	0.1103
556	A	A	0.0000
557	L	A	0.0000
558	F	A	0.0000
559	D	A	0.0000
560	I	A	0.0000
561	L	A	0.0000
562	S	A	-1.0476
563	G	A	-1.2670
564	K	A	-2.0629
565	R	A	-1.6335
566	A	A	-1.1383
567	P	A	0.0000
568	A	A	0.0000
569	G	A	0.0000
570	R	A	0.0000
571	L	A	0.0000
572	V	A	0.0000
573	T	A	0.0000
574	T	A	0.0000
575	Q	A	0.0000
576	Y	A	0.0000
577	P	A	-1.0157
578	A	A	-1.4485
579	E	A	-2.5827
580	Y	A	0.0000
581	V	A	0.0000
582	H	A	-2.3081
583	Q	A	-2.1925
584	F	A	0.0000
585	P	A	-0.9189
586	Q	A	0.0000
587	N	A	-0.5432
588	D	A	-0.2994
589	M	A	0.0000
590	N	A	-0.8213
591	L	A	0.0000
592	R	A	-0.9012
593	P	A	-1.6781
594	D	A	-2.8061
595	G	A	-2.2469
596	K	A	-2.7166
597	S	A	-1.8768
598	N	A	0.0000
599	P	A	-1.1488
600	G	A	0.0000
601	Q	A	0.0000
602	T	A	0.0000
603	Y	A	0.0000
604	I	A	-0.1163
605	W	A	0.0000
606	Y	A	0.0000
607	T	A	-0.6243
608	G	A	-1.0146
609	K	A	-1.9552
610	P	A	-1.2740
611	V	A	0.0000
612	Y	A	0.0000
613	E	A	-1.2606
614	F	A	0.0000
615	G	A	0.0000
616	S	A	-0.5084
617	G	A	-0.0290
618	L	A	0.6763
619	F	A	0.3842
620	Y	A	-0.3866
621	T	A	-0.8535
622	T	A	-0.8814
623	F	A	-1.2216
624	K	A	-2.1651
625	E	A	-1.4491
626	T	A	-0.8585
627	L	A	0.1646
628	A	A	-0.2437
634	L	A	0.2545
635	K	A	-1.4527
636	F	A	-0.4506
637	N	A	-1.0957
638	T	A	0.0000
639	S	A	-0.7666
640	S	A	-0.1704
641	I	A	0.1655
642	L	A	0.1160
643	S	A	-0.1907
644	A	A	-0.1524
645	P	A	-0.3657
646	H	A	-0.3685
647	P	A	-0.4310
648	G	A	-0.3468
649	Y	A	0.2797
650	T	A	0.2077
651	Y	A	0.3165
652	S	A	0.1092
653	E	A	0.0000
654	Q	A	-0.1344
655	I	A	0.0000
656	P	A	-0.0706
657	V	A	0.0000
658	F	A	0.3240
659	T	A	-0.2803
660	F	A	0.0000
661	E	A	-1.1619
662	A	A	0.0000
663	N	A	-1.5710
664	I	A	0.0000
665	K	A	-2.4386
666	N	A	0.0000
667	S	A	-1.8049
668	G	A	-1.8416
669	K	A	-2.5180
670	T	A	-2.2438
671	E	A	-3.4085
672	S	A	0.0000
673	P	A	0.0000
674	Y	A	0.0000
675	T	A	0.0000
676	A	A	0.0000
677	M	A	0.0000
678	L	A	0.0000
679	F	A	0.0000
680	V	A	0.0000
681	R	A	-1.2636
682	T	A	0.0000
683	S	A	-1.2004
684	N	A	-1.6763
685	A	A	0.0000
686	G	A	-0.7146
687	P	A	-0.4128
688	A	A	-0.2405
689	P	A	-0.2465
690	Y	A	-0.1934
691	P	A	-0.3313
692	N	A	-0.7231
693	K	A	-0.7211
694	W	A	0.0000
695	L	A	0.0000
696	V	A	0.0000
697	G	A	0.0000
698	F	A	0.0000
699	D	A	-0.6667
700	R	A	-0.6758
701	L	A	-0.6900
702	A	A	-1.0960
703	D	A	-2.6713
704	I	A	0.0000
705	K	A	-3.4655
706	P	A	-2.7081
707	G	A	-2.0568
708	H	A	-2.4325
709	S	A	-2.0773
710	S	A	-1.7933
711	K	A	-2.1681
712	L	A	0.0000
713	S	A	-1.0534
714	I	A	0.0000
715	P	A	-0.3292
716	I	A	0.0000
717	P	A	-0.1788
718	V	A	0.0000
719	S	A	-0.0186
720	A	A	0.0000
721	L	A	0.0000
722	A	A	0.0000
723	R	A	0.0000
724	V	A	0.0000
725	D	A	-0.9178
726	S	A	-1.0338
727	H	A	-1.3482
728	G	A	0.0000
729	N	A	-1.1778
730	R	A	0.0000
731	I	A	-0.4477
732	V	A	0.0000
733	Y	A	0.0000
734	P	A	-1.4867
735	G	A	-1.5010
736	K	A	-2.3811
737	Y	A	0.0000
738	E	A	-2.0507
739	L	A	0.0000
740	A	A	0.0000
741	L	A	0.0000
742	N	A	0.0000
743	T	A	-1.2533
744	D	A	-2.0630
745	E	A	-1.7727
746	S	A	-1.4903
747	V	A	0.0000
748	K	A	-1.9593
749	L	A	-1.2626
750	E	A	-2.5680
751	F	A	0.0000
752	E	A	-1.7475
753	L	A	0.0000
754	V	A	-1.2342
755	G	A	-1.7381
756	E	A	-2.8234
757	E	A	-2.6378
758	V	A	-1.1411
759	T	A	-0.7471
760	I	A	-0.2556
761	E	A	-0.7589
762	N	A	-1.3503
763	W	A	0.0000
764	P	A	-0.7430
765	L	A	-0.0795
766	E	A	-1.7911
767	E	A	-2.1908

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Automated mutations analysis

In the automated mutations mode, the server selects aggregation prone resides and each selected residue is mutated to glutamic acid, lysine, aspartic acid and arginine. The table below shows 2 best scored mutants for each mutated residue. Protein variants are ordered according to the mutation effect they had on protein stability (energetic effect) together with the difference in the average per-residue aggregation score between the wild type and the mutant (in the table green values indicate a positive change, grey are neutral, and orange/red mean destabilizing or more aggregation prone mutants).
Summary for all the mutants can be found in this CSV file.

Mutant	Energetic effect	Score comparison
VR337A	-0.6735	-0.0066	View	CSV	PDB
VR194A	-0.7149	-0.0047	View	CSV	PDB
VK337A	-0.3939	-0.0061	View	CSV	PDB
IR227A	-0.323	-0.0061	View	CSV	PDB
VE194A	-0.0473	-0.0091	View	CSV	PDB
LK618A	-0.2124	-0.0011	View	CSV	PDB
YK6A	0.0756	-0.0084	View	CSV	PDB
YD6A	0.1426	-0.0067	View	CSV	PDB
LR618A	0.1514	-0.0054	View	CSV	PDB
IK227A	0.3074	-0.0102	View	CSV	PDB
YK435A	1.777	-0.0066	View	CSV	PDB
YR435A	1.7569	-0.0042	View	CSV	PDB

Project name: c6fa987f9f5c124

Status: done

Started: 2026-04-03 10:44:24

Automated mutations analysis

Mutant

Energetic effect

Score comparison