Project name: seed10

Chain sequence(s)	A: NASCEYSASHKQDLLLGSTGSISCPSLSCQSDAQSPAVTWYKNGKLLSVERSNRIVVDEVYDYHQGTYVCDYTQSDTVSSWTVRAVVQVRTIVGDTKDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALGAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK; B: NASCEYSASHKQDLLLGSTGSISCPSLSCQSDAQSPAVTWYKNGKLLSVERSNRIVVDEVYDYHQGTYVCDYTQSDTVSSWTVRAVVQVRTIVGDTKDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALGAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
Selected Chain(s)	A,B
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:01)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:01)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:01)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:01)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:01)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:12:51)
[INFO]       Main:     Simulation completed successfully.                                          (00:13:00)

• Minimal score value: -3.6696
• Maximal score value: 1.5119
• Average score: -0.7183
• Total score value: -465.4359

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	N	A	-1.2488
2	A	A	-0.8368
3	S	A	-0.6985
4	C	A	-0.2258
5	E	A	-0.9779
6	Y	A	0.5805
7	S	A	-0.0489
8	A	A	-0.4615
9	S	A	-1.3347
10	H	A	-2.0965
11	K	A	-2.9456
12	Q	A	-2.2484
13	D	A	-1.8402
14	L	A	0.0000
15	L	A	0.2431
16	L	A	0.4601
17	G	A	0.0000
18	S	A	0.0000
19	T	A	-0.6115
20	G	A	0.0000
21	S	A	0.1514
22	I	A	0.0000
23	S	A	-0.0866
24	C	A	0.0000
25	P	A	0.0000
26	S	A	-0.0897
27	L	A	0.0000
28	S	A	0.0050
29	C	A	0.2471
30	Q	A	-0.6430
31	S	A	-0.9265
32	D	A	-1.2030
33	A	A	-0.9415
34	Q	A	-0.9507
35	S	A	0.0000
36	P	A	-0.7414
37	A	A	0.0000
38	V	A	0.0000
39	T	A	0.0000
40	W	A	0.0000
41	Y	A	0.0000
42	K	A	-1.1254
43	N	A	-1.7829
44	G	A	-1.2274
45	K	A	-1.8269
46	L	A	-0.3982
47	L	A	0.4024
48	S	A	0.2140
49	V	A	0.0000
50	E	A	-0.7784
51	R	A	-1.1240
52	S	A	-0.8288
53	N	A	0.0000
54	R	A	-0.2849
55	I	A	0.0000
56	V	A	1.1681
57	V	A	0.0000
58	D	A	-1.4733
59	E	A	-0.9273
60	V	A	0.0000
61	Y	A	0.2457
62	D	A	0.0980
63	Y	A	0.6888
64	H	A	0.0000
65	Q	A	-1.4878
66	G	A	-1.2009
67	T	A	-1.2900
68	Y	A	0.0000
69	V	A	0.0000
70	C	A	0.0000
71	D	A	0.0000
72	Y	A	0.0000
73	T	A	-0.3423
74	Q	A	-0.9458
75	S	A	-0.8533
76	D	A	-1.1034
77	T	A	0.0257
78	V	A	1.1930
79	S	A	0.1969
80	S	A	-0.2718
81	W	A	-0.0649
82	T	A	-0.4839
83	V	A	-0.1485
84	R	A	-0.2055
85	A	A	0.0000
86	V	A	-0.2358
87	V	A	0.0000
88	Q	A	-2.0834
89	V	A	0.0000
90	R	A	-2.1141
91	T	A	-0.4194
92	I	A	0.5562
93	V	A	0.8303
94	G	A	-0.7042
95	D	A	-2.2927
96	T	A	-2.3174
97	K	A	-3.3077
98	D	A	-3.6655
99	K	A	-3.3189
100	T	A	-1.8661
101	H	A	-1.6945
102	T	A	-0.6167
103	C	A	0.2020
104	P	A	-0.0664
105	P	A	0.1639
106	C	A	0.7027
107	P	A	-0.2884
108	A	A	-0.4842
109	P	A	-1.0493
110	E	A	-1.9727
111	A	A	-1.0502
112	A	A	-0.6638
113	G	A	-0.8136
114	G	A	-0.8163
115	P	A	0.0000
116	S	A	-0.3595
117	V	A	0.0000
118	F	A	0.4985
119	L	A	0.2688
120	F	A	0.0643
121	P	A	-0.1900
122	P	A	0.0000
123	K	A	-0.9486
124	P	A	-0.6765
125	K	A	-0.5902
126	D	A	-0.5383
127	T	A	0.0000
128	L	A	0.0000
129	M	A	0.4678
130	I	A	1.5119
131	S	A	0.3972
132	R	A	-0.4556
133	T	A	-0.1859
134	P	A	0.0000
135	E	A	-0.8265
136	V	A	0.0000
137	T	A	0.0000
138	C	A	0.0000
139	V	A	0.0000
140	V	A	0.0000
141	V	A	0.0000
142	D	A	-1.1029
143	V	A	0.0000
144	S	A	-2.1400
145	H	A	-2.6133
146	E	A	-2.9874
147	D	A	-2.6242
148	P	A	-2.7000
149	E	A	-3.1950
150	V	A	-2.0400
151	K	A	-2.2339
152	F	A	-1.1004
153	N	A	-1.0021
154	W	A	0.0000
155	Y	A	-0.6343
156	V	A	-0.9206
157	D	A	-2.1317
158	G	A	-0.9091
159	V	A	0.5437
160	E	A	-0.7120
161	V	A	-0.5547
162	H	A	-1.8957
163	N	A	-2.1895
164	A	A	-1.7962
165	K	A	-2.2199
166	T	A	-1.5734
167	K	A	-1.1873
168	P	A	-1.5750
169	R	A	-2.6748
170	E	A	0.0000
171	E	A	-2.3796
172	Q	A	0.0000
173	Y	A	0.0625
174	N	A	-0.8113
175	S	A	-0.8923
176	T	A	-1.4643
177	Y	A	0.0000
178	R	A	0.0000
179	V	A	0.0000
180	V	A	0.0000
181	S	A	0.0000
182	V	A	0.0000
183	L	A	0.0000
184	T	A	-0.5965
185	V	A	0.0000
186	L	A	0.9281
187	H	A	0.0152
188	Q	A	-1.0614
189	D	A	-1.0611
190	W	A	0.0000
191	L	A	-0.9601
192	N	A	-1.8649
193	G	A	-1.7412
194	K	A	-2.0970
195	E	A	-2.2206
196	Y	A	0.0000
197	K	A	-1.2338
198	C	A	0.0000
199	K	A	-0.9663
200	V	A	0.0000
201	S	A	-1.3852
202	N	A	0.0000
203	K	A	-2.5743
204	A	A	-1.4840
205	L	A	-0.9299
206	G	A	-0.7163
207	A	A	-0.4448
208	P	A	-0.7691
209	I	A	-0.5545
210	E	A	-0.8049
211	K	A	-0.6423
212	T	A	-0.7921
213	I	A	0.0000
214	S	A	0.0000
215	K	A	0.0000
216	A	A	-0.8451
217	K	A	-0.4226
218	G	A	-0.8949
219	Q	A	-2.1007
220	P	A	-1.7316
221	R	A	-2.3832
222	E	A	-2.7669
223	P	A	0.0000
224	Q	A	-1.2873
225	V	A	0.0000
226	Y	A	0.0000
227	T	A	-0.7715
228	L	A	0.0000
229	P	A	-0.4033
230	P	A	-0.8764
231	S	A	0.0000
232	R	A	-2.6706
233	D	A	-2.7876
234	E	A	0.0000
235	L	A	-1.7857
236	T	A	-1.5172
237	K	A	-2.0322
238	N	A	-2.3902
239	Q	A	-2.1999
240	V	A	0.0000
241	S	A	0.0000
242	L	A	0.0000
243	T	A	0.0000
244	C	A	0.0000
245	L	A	0.0000
246	V	A	0.0000
247	K	A	-0.7399
248	G	A	-1.3187
249	F	A	0.0000
250	Y	A	0.0000
251	P	A	0.0000
252	S	A	-0.8166
253	D	A	-0.9569
254	I	A	0.0000
255	A	A	-0.4348
256	V	A	0.0000
257	E	A	-1.0617
258	W	A	0.0000
259	E	A	-1.6810
260	S	A	0.0000
261	N	A	-1.8280
262	G	A	-1.7359
263	Q	A	-2.2532
264	P	A	-1.8780
265	E	A	0.0000
266	N	A	-1.9926
267	N	A	-1.5480
268	Y	A	-1.1108
269	K	A	-1.0750
270	T	A	-0.4359
271	T	A	0.0000
272	P	A	-0.2449
273	P	A	-0.2434
274	V	A	-0.1011
275	L	A	-0.2191
276	D	A	-1.1431
277	S	A	-1.4482
278	D	A	-2.1238
279	G	A	-1.3019
280	S	A	0.0000
281	F	A	0.0000
282	F	A	0.0000
283	L	A	0.0000
284	Y	A	0.0000
285	S	A	0.0000
286	K	A	0.0000
287	L	A	0.0000
288	T	A	-0.9234
289	V	A	0.0000
290	D	A	-2.4019
291	K	A	-2.4731
292	S	A	-2.1852
293	R	A	-1.9521
294	W	A	0.0000
295	Q	A	-2.3426
296	Q	A	-2.1204
297	G	A	-1.1800
298	N	A	-0.8094
299	V	A	0.2705
300	F	A	0.0000
301	S	A	0.0000
302	C	A	0.0000
303	S	A	0.0000
304	V	A	0.0000
305	M	A	0.0000
306	H	A	0.0000
307	E	A	-1.1448
308	A	A	-1.6085
309	L	A	-1.4407
310	H	A	-1.7419
311	N	A	-1.4185
312	H	A	-0.9007
313	Y	A	-0.3592
314	T	A	-0.6026
315	Q	A	-0.8831
316	K	A	-0.9305
317	S	A	-0.4185
318	L	A	0.0000
319	S	A	-0.1113
320	L	A	-0.3487
321	S	A	-0.6049
322	P	A	-1.2333
323	G	A	-1.4261
324	K	A	-2.0333
1	N	B	-1.2331
2	A	B	-0.8007
3	S	B	-0.6565
4	C	B	-0.1660
5	E	B	-0.9482
6	Y	B	0.5793
7	S	B	-0.1026
8	A	B	-0.5022
9	S	B	-1.3719
10	H	B	-2.1383
11	K	B	-2.9911
12	Q	B	-2.2992
13	D	B	-1.8916
14	L	B	0.0000
15	L	B	0.2537
16	L	B	0.3932
17	G	B	0.0000
18	S	B	0.0000
19	T	B	-0.6053
20	G	B	0.0000
21	S	B	0.1214
22	I	B	0.0000
23	S	B	-0.1723
24	C	B	0.0000
25	P	B	0.0000
26	S	B	-0.1659
27	L	B	0.0000
28	S	B	-0.0836
29	C	B	0.1414
30	Q	B	-0.5921
31	S	B	-0.8060
32	D	B	-1.0199
33	A	B	-0.7980
34	Q	B	-0.7715
35	S	B	0.0000
36	P	B	-0.5642
37	A	B	0.0000
38	V	B	0.0000
39	T	B	0.0000
40	W	B	0.0000
41	Y	B	0.0000
42	K	B	-1.1354
43	N	B	-1.7848
44	G	B	-1.2096
45	K	B	-1.7199
46	L	B	-0.1811
47	L	B	0.5013
48	S	B	0.2211
49	V	B	0.0000
50	E	B	-0.6117
51	R	B	-0.8107
52	S	B	-0.6799
53	N	B	0.0000
54	R	B	-0.3658
55	I	B	0.0000
56	V	B	1.1151
57	V	B	0.0000
58	D	B	-1.5603
59	E	B	-1.1076
60	V	B	0.0000
61	Y	B	0.1022
62	D	B	-0.0267
63	Y	B	0.4673
64	H	B	-0.4789
65	Q	B	-1.7135
66	G	B	-1.3262
67	T	B	-1.3496
68	Y	B	0.0000
69	V	B	0.0000
70	C	B	0.0000
71	D	B	0.0000
72	Y	B	0.0000
73	T	B	0.0000
74	Q	B	-0.9719
75	S	B	-0.9558
76	D	B	-1.3331
77	T	B	-0.0887
78	V	B	1.1114
79	S	B	0.0854
80	S	B	-0.3281
81	W	B	-0.1279
82	T	B	-0.5535
83	V	B	-0.2215
84	R	B	-0.5210
85	A	B	0.0000
86	V	B	-0.2794
87	V	B	0.0000
88	Q	B	-2.1611
89	V	B	0.0000
90	R	B	-2.2933
91	T	B	-0.5276
92	I	B	0.5560
93	V	B	0.7357
94	G	B	-0.6901
95	D	B	-2.2937
96	T	B	-2.3925
97	K	B	-3.2822
98	D	B	-3.6696
99	K	B	-3.3065
100	T	B	-1.8889
101	H	B	-1.7072
102	T	B	-0.7025
103	C	B	0.1532
104	P	B	-0.0959
105	P	B	0.1421
106	C	B	0.5603
107	P	B	-0.3528
108	A	B	-0.4695
109	P	B	-1.0378
110	E	B	-1.9406
111	A	B	-1.0345
112	A	B	-0.6536
113	G	B	-0.8344
114	G	B	-1.0169
115	P	B	0.0000
116	S	B	-0.4352
117	V	B	0.0000
118	F	B	0.4441
119	L	B	0.3194
120	F	B	0.0055
121	P	B	-0.2444
122	P	B	0.0000
123	K	B	-1.1141
124	P	B	0.0000
125	K	B	-0.4827
126	D	B	0.0000
127	T	B	0.0000
128	L	B	0.0000
129	M	B	0.4700
130	I	B	1.4599
131	S	B	0.2594
132	R	B	-0.7377
133	T	B	-0.3071
134	P	B	0.0000
135	E	B	-0.7935
136	V	B	0.0000
137	T	B	-0.1565
138	C	B	0.0000
139	V	B	0.0000
140	V	B	0.0000
141	V	B	0.0000
142	D	B	-1.2951
143	V	B	0.0000
144	S	B	-2.1308
145	H	B	-2.5916
146	E	B	-2.9634
147	D	B	-2.5663
148	P	B	-2.6529
149	E	B	-3.1526
150	V	B	-2.0124
151	K	B	-2.2467
152	F	B	-1.1024
153	N	B	-0.9941
154	W	B	0.0000
155	Y	B	-0.5818
156	V	B	-0.8728
157	D	B	-2.0858
158	G	B	-0.8703
159	V	B	0.5873
160	E	B	-0.6453
161	V	B	-0.5278
162	H	B	-1.8831
163	N	B	-2.1648
164	A	B	-1.7549
165	K	B	-2.1979
166	T	B	-1.5799
167	K	B	-1.2259
168	P	B	-1.5633
169	R	B	-2.5561
170	E	B	0.0000
171	E	B	-2.2294
172	Q	B	0.0000
173	Y	B	0.1392
174	N	B	-0.8160
175	S	B	-0.8627
176	T	B	-1.4599
177	Y	B	-1.6461
178	R	B	0.0000
179	V	B	0.0000
180	V	B	0.0000
181	S	B	0.0000
182	V	B	-0.7824
183	L	B	0.0000
184	T	B	-0.5423
185	V	B	0.0000
186	L	B	0.9213
187	H	B	0.0020
188	Q	B	-1.0811
189	D	B	-1.0844
190	W	B	0.0000
191	L	B	-0.9838
192	N	B	-1.8708
193	G	B	-1.7275
194	K	B	-2.0833
195	E	B	-2.1136
196	Y	B	0.0000
197	K	B	-1.1740
198	C	B	0.0000
199	K	B	-1.0316
200	V	B	0.0000
201	S	B	-1.4389
202	N	B	0.0000
203	K	B	-2.5893
204	A	B	-1.4771
205	L	B	-0.9474
206	G	B	-0.7350
207	A	B	-0.6687
208	P	B	-0.9013
209	I	B	-0.6652
210	E	B	-0.8389
211	K	B	-0.6203
212	T	B	-0.8094
213	I	B	0.0000
214	S	B	-1.0588
215	K	B	0.0000
216	A	B	-0.6372
217	K	B	-0.3099
218	G	B	-0.7428
219	Q	B	-1.8464
220	P	B	-1.5861
221	R	B	-2.0848
222	E	B	-2.6551
223	P	B	0.0000
224	Q	B	-1.2318
225	V	B	0.0000
226	Y	B	0.0000
227	T	B	-0.7578
228	L	B	0.0000
229	P	B	-0.4050
230	P	B	-0.8671
231	S	B	-1.5284
232	R	B	-2.6548
233	D	B	-2.7994
234	E	B	0.0000
235	L	B	-1.7476
236	T	B	-1.5515
237	K	B	-2.1326
238	N	B	-2.3915
239	Q	B	-2.1345
240	V	B	0.0000
241	S	B	0.0000
242	L	B	0.0000
243	T	B	0.0000
244	C	B	0.0000
245	L	B	0.0000
246	V	B	0.0000
247	K	B	-0.7413
248	G	B	-1.2526
249	F	B	0.0000
250	Y	B	0.0000
251	P	B	0.0000
252	S	B	-0.7539
253	D	B	-0.8643
254	I	B	0.0000
255	A	B	0.0000
256	V	B	0.0000
257	E	B	-1.0060
258	W	B	0.0000
259	E	B	-1.7156
260	S	B	0.0000
261	N	B	-1.8996
262	G	B	-1.7661
263	Q	B	-2.2517
264	P	B	-1.8585
265	E	B	0.0000
266	N	B	-1.9915
267	N	B	-1.4695
268	Y	B	-1.0483
269	K	B	-1.0424
270	T	B	-0.4209
271	T	B	0.0000
272	P	B	-0.2322
273	P	B	-0.2153
274	V	B	-0.0545
275	L	B	-0.1361
276	D	B	-1.0834
277	S	B	-1.4019
278	D	B	-2.0483
279	G	B	-1.1073
280	S	B	0.0000
281	F	B	0.0000
282	F	B	0.0000
283	L	B	0.0000
284	Y	B	0.0000
285	S	B	0.0000
286	K	B	0.0000
287	L	B	0.0000
288	T	B	-0.8528
289	V	B	0.0000
290	D	B	-2.2243
291	K	B	-2.3809
292	S	B	-2.1217
293	R	B	-1.9397
294	W	B	0.0000
295	Q	B	-2.2932
296	Q	B	-2.1500
297	G	B	-1.3179
298	N	B	-1.0115
299	V	B	-0.0708
300	F	B	0.0000
301	S	B	0.0000
302	C	B	0.0000
303	S	B	0.0000
304	V	B	0.0000
305	M	B	0.0000
306	H	B	0.0000
307	E	B	-1.1190
308	A	B	-1.5979
309	L	B	0.0000
310	H	B	-1.8426
311	N	B	-1.4880
312	H	B	-0.9855
313	Y	B	-0.5154
314	T	B	-0.7027
315	Q	B	-0.9438
316	K	B	-0.8980
317	S	B	-0.4808
318	L	B	0.0000
319	S	B	-0.2535
320	L	B	-0.3944
321	S	B	-0.6408
322	P	B	-1.1168
323	G	B	-1.4220
324	K	B	-2.0348