Project name: GS linker_20

Chain sequence(s)	A: GGGGSGGGGSGGGGS; C: GGGGSGGGGSGGGGS; B: GGGGSGGGGSGGGGS; E: GGGGSGGGGSGGGGS; D: GGGGSGGGGSGGGGS; G: GGGGSGGGGSGGGGS; F: GGGGSGGGGSGGGGS; I: GGGGSGGGGSGGGGS; H: GGGGSGGGGSGGGGS; K: GGGGSGGGGSGGGGS; J: GGGGSGGGGSGGGGS; M: GGGGSGGGGSGGGGS; L: GGGGSGGGGSGGGGS; O: GGGGSGGGGSGGGGS; N: GGGGSGGGGSGGGGS; Q: GGGGSGGGGSGGGGS; P: GGGGSGGGGSGGGGS; S: GGGGSGGGGSGGGGS; R: GGGGSGGGGSGGGGS; T: GGGGSGGGGSGGGGS
Selected Chain(s)	A,C,B,E,D,G,F,I,H,K,J,M,L,O,N,Q,P,S,R,T
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:00:05)
[INFO]       Main:     Simulation completed successfully.                                          (00:00:10)

• Minimal score value: -2.0134
• Maximal score value: 0.0
• Average score: -1.3859
• Total score value: -415.7813

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	G	A	-1.4400
2	G	A	-1.4200
3	G	A	-1.7122
4	G	A	-1.8148
5	S	A	-1.8269
6	G	A	-1.7399
7	G	A	-1.9507
8	G	A	-1.8618
9	G	A	-1.6562
10	S	A	-1.5087
11	G	A	-1.5522
12	G	A	-1.7475
13	G	A	-1.7753
14	G	A	-1.6888
15	S	A	-1.5280
1	G	B	-1.5380
2	G	B	0.0000
3	G	B	-1.7319
4	G	B	-1.7018
5	S	B	-1.5261
6	G	B	-1.7746
7	G	B	-1.5467
8	G	B	-1.3902
9	G	B	-1.2507
10	S	B	-1.1183
11	G	B	-1.5482
12	G	B	-1.5156
13	G	B	-1.4044
14	G	B	-1.5335
15	S	B	-0.9954
1	G	C	-1.1845
2	G	C	-1.3227
3	G	C	-1.4185
4	G	C	-1.3635
5	S	C	-1.2951
6	G	C	-1.6159
7	G	C	-1.9003
8	G	C	-1.9787
9	G	C	-2.0134
10	S	C	-1.6954
11	G	C	-1.8809
12	G	C	-1.6817
13	G	C	-1.3278
14	G	C	-1.4722
15	S	C	-1.0489
1	G	D	-1.1949
2	G	D	-1.2871
3	G	D	-1.4622
4	G	D	-1.3061
5	S	D	-1.4874
6	G	D	-1.6909
7	G	D	-1.5211
8	G	D	-1.5393
9	G	D	0.0000
10	S	D	-1.6337
11	G	D	0.0000
12	G	D	0.0000
13	G	D	0.0000
14	G	D	0.0000
15	S	D	-1.0001
1	G	E	-1.3558
2	G	E	-1.5285
3	G	E	-1.7212
4	G	E	-1.7389
5	S	E	-1.6995
6	G	E	-1.8735
7	G	E	-1.8003
8	G	E	-1.7755
9	G	E	-1.4988
10	S	E	-1.3303
11	G	E	-1.7221
12	G	E	-1.6148
13	G	E	-1.6558
14	G	E	-1.4616
15	S	E	-0.9670
1	G	F	-1.2279
2	G	F	-1.5591
3	G	F	-1.5495
4	G	F	-1.4011
5	S	F	-1.2189
6	G	F	-1.4342
7	G	F	-1.6393
8	G	F	-1.8165
9	G	F	-1.6844
10	S	F	-1.4601
11	G	F	-1.4180
12	G	F	0.0000
13	G	F	-1.5318
14	G	F	-1.4906
15	S	F	-1.1224
1	G	G	-1.0891
2	G	G	-1.3791
3	G	G	-1.5002
4	G	G	-1.6152
5	S	G	-1.5605
6	G	G	-1.5088
7	G	G	-1.5730
8	G	G	-1.7095
9	G	G	-1.5870
10	S	G	-1.3298
11	G	G	-1.4276
12	G	G	-1.4232
13	G	G	-1.3437
14	G	G	-1.3297
15	S	G	-0.7879
1	G	H	-1.3108
2	G	H	-1.5938
3	G	H	-1.5719
4	G	H	-1.5356
5	S	H	-1.4197
6	G	H	-1.5927
7	G	H	-1.6985
8	G	H	-1.5143
9	G	H	-1.2419
10	S	H	-1.1675
11	G	H	-1.3831
12	G	H	-1.4553
13	G	H	-1.5959
14	G	H	-1.1590
15	S	H	-0.6127
1	G	I	-1.6734
2	G	I	-1.5933
3	G	I	-1.6566
4	G	I	-1.6477
5	S	I	-1.4606
6	G	I	-1.7622
7	G	I	-1.7775
8	G	I	-1.4889
9	G	I	-1.4601
10	S	I	-1.3031
11	G	I	-1.5985
12	G	I	-1.5376
13	G	I	-1.4948
14	G	I	-1.6429
15	S	I	-1.4345
1	G	J	-1.2124
2	G	J	-1.5199
3	G	J	-1.6948
4	G	J	-1.6151
5	S	J	-1.4375
6	G	J	-1.5669
7	G	J	-1.6437
8	G	J	-1.8325
9	G	J	-1.6855
10	S	J	-1.6754
11	G	J	-1.9156
12	G	J	0.0000
13	G	J	-1.6338
14	G	J	-1.3987
15	S	J	-0.8415
1	G	K	-1.2413
2	G	K	-1.3272
3	G	K	-1.4972
4	G	K	-1.4244
5	S	K	-1.3491
6	G	K	-1.6075
7	G	K	-1.6884
8	G	K	-1.7986
9	G	K	-1.7692
10	S	K	-1.2786
11	G	K	-1.4826
12	G	K	-1.3607
13	G	K	-1.2641
14	G	K	-1.3587
15	S	K	-0.8926
1	G	L	-1.2172
2	G	L	-1.1160
3	G	L	-1.7192
4	G	L	-1.7638
5	S	L	-1.7211
6	G	L	-1.7363
7	G	L	-1.8406
8	G	L	-1.7480
9	G	L	-1.5060
10	S	L	-1.1614
11	G	L	-1.6368
12	G	L	-1.3689
13	G	L	-1.6022
14	G	L	-1.5281
15	S	L	-1.3849
1	G	M	-1.1411
2	G	M	-1.4869
3	G	M	-1.5694
4	G	M	-1.6944
5	S	M	-1.5122
6	G	M	-1.4170
7	G	M	-1.2479
8	G	M	-1.1762
9	G	M	-1.2305
10	S	M	-1.2931
11	G	M	-1.3328
12	G	M	-1.6749
13	G	M	-1.4996
14	G	M	-1.2260
15	S	M	-0.9112
1	G	N	-1.1482
2	G	N	-1.2517
3	G	N	-1.4388
4	G	N	-1.3398
5	S	N	-1.5119
6	G	N	-1.5906
7	G	N	-1.5362
8	G	N	-1.5329
9	G	N	-1.4145
10	S	N	-1.4530
11	G	N	-1.4286
12	G	N	-1.4237
13	G	N	-1.4338
14	G	N	-1.0080
15	S	N	-0.5950
1	G	O	-1.1944
2	G	O	-1.4244
3	G	O	-1.4590
4	G	O	-1.6539
5	S	O	-1.6463
6	G	O	-1.7165
7	G	O	-1.6835
8	G	O	-1.5555
9	G	O	-1.2310
10	S	O	-1.3330
11	G	O	0.0000
12	G	O	0.0000
13	G	O	-1.4433
14	G	O	-1.2553
15	S	O	-1.0219
1	G	P	-1.5400
2	G	P	-1.6569
3	G	P	-1.8008
4	G	P	-1.7979
5	S	P	-1.6364
6	G	P	-1.5352
7	G	P	-1.6832
8	G	P	0.0000
9	G	P	-1.6042
10	S	P	0.0000
11	G	P	-1.5859
12	G	P	-1.7297
13	G	P	-1.7907
14	G	P	-1.4542
15	S	P	-0.9703
1	G	Q	-1.2181
2	G	Q	-1.4139
3	G	Q	-1.6204
4	G	Q	-1.6691
5	S	Q	-1.5472
6	G	Q	-1.6473
7	G	Q	-1.7160
8	G	Q	-1.7633
9	G	Q	-1.4780
10	S	Q	-1.2506
11	G	Q	-1.4470
12	G	Q	-1.4719
13	G	Q	-1.3753
14	G	Q	-1.3690
15	S	Q	-0.8501
1	G	R	-0.9933
2	G	R	-1.2726
3	G	R	-1.4551
4	G	R	-1.4571
5	S	R	-1.5023
6	G	R	0.0000
7	G	R	-1.4845
8	G	R	0.0000
9	G	R	-1.4284
10	S	R	-1.4273
11	G	R	-1.2609
12	G	R	-1.4700
13	G	R	-1.4491
14	G	R	-1.1010
15	S	R	-0.7311
1	G	S	-1.5562
2	G	S	-1.5945
3	G	S	-1.5463
4	G	S	-1.6832
5	S	S	-1.5585
6	G	S	-1.7257
7	G	S	-1.5167
8	G	S	-1.6214
9	G	S	-1.4480
10	S	S	-1.5296
11	G	S	-1.5361
12	G	S	-1.5980
13	G	S	-1.5225
14	G	S	-1.3341
15	S	S	-0.6992
1	G	T	-1.1026
2	G	T	-1.2883
3	G	T	0.0000
4	G	T	-1.5438
5	S	T	-1.4494
6	G	T	0.0000
7	G	T	-1.6676
8	G	T	-1.4018
9	G	T	-1.2899
10	S	T	0.0000
11	G	T	-1.4300
12	G	T	-1.3167
13	G	T	-1.1747
14	G	T	-1.0789
15	S	T	-0.7436