Project name: obj1 [mutate: SA85C, LH86C, AM88C, YF80C]

Chain sequence(s)	C: EVQLVESGGGLVQPGGSLRLSCAASDFTFRSYEMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARLRDGFNKGFDYWGQGTLVTVSS
Selected Chain(s)	C
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No
Mutated residues	AM88C,YF80C,SA85C,LH86C
Energy difference between WT (input) and mutated protein (by FoldX)	5.07755 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with C chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       FoldX:    Building mutant model                                                       (00:00:24)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:53)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:01:20)
[INFO]       Main:     Simulation completed successfully.                                          (00:01:21)

• Minimal score value: -3.3333
• Maximal score value: 1.7634
• Average score: -0.6145
• Total score value: -73.7389

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	C	-2.0063
2	V	C	-0.9165
3	Q	C	-1.2436
4	L	C	0.0000
5	V	C	0.5640
6	E	C	0.2402
7	S	C	-0.3968
8	G	C	-0.7767
9	G	C	0.0408
10	G	C	0.9020
11	L	C	1.4211
12	V	C	0.0262
13	Q	C	-1.2172
14	P	C	-1.2840
15	G	C	-1.2494
16	G	C	-0.9160
17	S	C	-1.1647
18	L	C	-0.8874
19	R	C	-2.0901
20	L	C	0.0000
21	S	C	-0.4862
22	C	C	0.0000
23	A	C	-0.1824
24	A	C	0.0000
25	S	C	-0.2007
26	D	C	0.0000
27	F	C	1.5458
28	T	C	0.2525
29	F	C	0.0000
30	R	C	-1.9945
31	S	C	-0.8863
32	Y	C	-1.2160
33	E	C	-1.1454
34	M	C	0.0000
35	S	C	0.0000
36	W	C	0.0000
37	V	C	0.0000
38	R	C	0.0000
39	Q	C	-0.2966
40	A	C	-0.9851
41	P	C	-1.2937
42	G	C	-1.4348
43	K	C	-2.1350
44	G	C	-1.0592
45	L	C	0.3948
46	E	C	-0.3909
47	W	C	0.3405
48	V	C	0.0000
49	S	C	0.0000
50	A	C	0.0000
51	I	C	0.0000
52	S	C	-0.5842
53	G	C	-1.2168
54	S	C	-1.1896
55	G	C	-1.0423
56	G	C	-0.7355
57	S	C	-0.3032
58	T	C	0.1978
59	Y	C	0.6077
60	Y	C	-0.3557
61	A	C	-1.1382
62	D	C	-2.3439
63	S	C	-1.7141
64	V	C	0.0000
65	K	C	-2.3844
66	G	C	-1.5975
67	R	C	0.0000
68	F	C	0.0000
69	T	C	-0.6654
70	I	C	0.0000
71	S	C	-0.4690
72	R	C	-1.1932
73	D	C	-1.6806
74	N	C	-2.0565
75	S	C	-1.7020
76	K	C	-2.2279
77	N	C	-1.5724
78	T	C	0.0000
79	L	C	0.0000
80	F	C	-0.4969	mutated: YF80C
81	L	C	0.0000
82	Q	C	-1.2135
83	M	C	0.0000
84	N	C	-1.2553
85	A	C	-1.0305	mutated: SA85C
86	H	C	0.0000	mutated: LH86C
87	R	C	-2.0737
88	M	C	-1.1704	mutated: AM88C
89	E	C	-1.9978
90	D	C	0.0000
91	T	C	-0.2159
92	A	C	0.0000
93	I	C	1.0770
94	Y	C	0.0000
95	Y	C	0.6448
96	C	C	0.0000
97	A	C	0.0000
98	R	C	0.0000
99	L	C	0.0000
100	R	C	-3.1907
101	D	C	-3.3333
102	G	C	-2.0866
103	F	C	-1.2310
104	N	C	-2.4286
105	K	C	-3.1848
106	G	C	-1.9076
107	F	C	-0.9859
108	D	C	-1.0994
109	Y	C	-0.2104
110	W	C	0.5562
111	G	C	-0.0696
112	Q	C	-0.8806
113	G	C	0.1091
114	T	C	0.5842
115	L	C	1.7634
116	V	C	0.0000
117	T	C	0.4571
118	V	C	0.0000
119	S	C	-0.5618
120	S	C	-0.8119