Project name: db407efe02e27ed

Chain sequence(s)	H: EVQLVESGGGLVQPGRSLRLSCAASGFTFDDYAMHWVRQAPGKGLEWVSAITWNSGHIDYADSVEGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCAKVSYLSTASSLDYWGQGTLVTVSS; L: DIQMTQSPSSLSASVGDRVTITCRASQGIRNYLAWYQQKPGKAPKLLIYAASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDVATYYCQRYNRAPYTFGQGTKVEIK
Selected Chain(s)	H,L
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:01)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:01)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:01)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:01)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:01)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:01:38)
[INFO]       Main:     Simulation completed successfully.                                          (00:01:40)

• Minimal score value: -2.8461
• Maximal score value: 1.9221
• Average score: -0.6466
• Total score value: -147.4147

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	H	-2.1323
2	V	H	-1.1704
3	Q	H	-1.2323
4	L	H	0.0000
5	V	H	1.0366
6	E	H	0.4013
7	S	H	-0.1423
8	G	H	-0.4120
9	G	H	0.1962
10	G	H	0.8463
11	L	H	1.5233
12	V	H	-0.0856
13	Q	H	-1.1572
14	P	H	-1.7161
15	G	H	-1.9973
16	R	H	-2.5595
17	S	H	-1.6210
18	L	H	-0.8297
19	R	H	-1.1291
20	L	H	0.0000
21	S	H	-0.1920
22	C	H	0.0000
23	A	H	-0.1650
24	A	H	0.0000
25	S	H	-1.0794
26	G	H	-1.2720
27	F	H	-0.8260
28	T	H	-0.8133
29	F	H	0.0000
30	D	H	-2.1201
31	D	H	-0.8882
32	Y	H	0.2215
33	A	H	0.0000
34	M	H	0.0000
35	H	H	0.0000
36	W	H	0.0000
37	V	H	0.0000
38	R	H	0.0000
39	Q	H	-0.7004
40	A	H	-1.0801
41	P	H	-0.8473
42	G	H	-1.4773
43	K	H	-2.3613
44	G	H	-1.6212
45	L	H	0.0000
46	E	H	-1.0240
47	W	H	0.0000
48	V	H	0.0000
49	S	H	0.0000
50	A	H	0.0000
51	I	H	0.0000
52	T	H	0.0000
53	W	H	-0.8903
54	N	H	-1.9282
55	S	H	-1.1482
56	G	H	-1.0940
57	H	H	-0.9242
58	I	H	0.3946
59	D	H	-0.4942
60	Y	H	-0.7596
61	A	H	0.0000
62	D	H	-2.6336
63	S	H	-1.7106
64	V	H	0.0000
65	E	H	-2.6558
66	G	H	-1.7493
67	R	H	-1.3017
68	F	H	0.0000
69	T	H	-0.5875
70	I	H	0.0000
71	S	H	-0.1221
72	R	H	-1.0923
73	D	H	-1.6233
74	N	H	-2.0891
75	A	H	-1.4875
76	K	H	-2.4477
77	N	H	-2.0887
78	S	H	-1.1666
79	L	H	0.0000
80	Y	H	-0.2754
81	L	H	0.0000
82	Q	H	-0.7193
83	M	H	0.0000
84	N	H	-1.5836
85	S	H	-1.6331
86	L	H	0.0000
87	R	H	-2.0539
88	A	H	-1.4432
89	E	H	-2.1185
90	D	H	0.0000
91	T	H	-0.3430
92	A	H	0.0000
93	V	H	0.5225
94	Y	H	0.0000
95	Y	H	0.0000
96	C	H	0.0000
97	A	H	0.0000
98	K	H	0.0000
99	V	H	0.0000
100	S	H	1.0596
101	Y	H	1.9129
102	L	H	1.9221
103	S	H	0.8347
104	T	H	0.1053
105	A	H	-0.2431
106	S	H	0.2906
107	S	H	0.0000
108	L	H	0.0000
109	D	H	0.1615
110	Y	H	0.3384
111	W	H	0.0000
112	G	H	0.0000
113	Q	H	-1.1933
114	G	H	-0.3605
115	T	H	0.4456
116	L	H	1.3812
117	V	H	0.0000
118	T	H	0.4565
119	V	H	0.0000
120	S	H	-0.4075
121	S	H	-0.3160
122	D	L	-2.4938
123	I	L	0.0000
124	Q	L	-2.3362
125	M	L	0.0000
126	T	L	-1.4600
127	Q	L	0.0000
128	S	L	-0.7395
129	P	L	-0.5508
130	S	L	-0.9421
131	S	L	-1.1597
132	L	L	-0.9113
133	S	L	-1.0969
134	A	L	-1.0794
135	S	L	-0.8257
136	V	L	0.0971
137	G	L	-0.6832
138	D	L	-1.7453
139	R	L	-2.3061
140	V	L	0.0000
141	T	L	-0.6494
142	I	L	0.0000
143	T	L	-0.8171
144	C	L	0.0000
145	R	L	-2.8461
146	A	L	0.0000
147	S	L	-2.1291
148	Q	L	-2.5900
149	G	L	-2.1503
150	I	L	0.0000
151	R	L	-2.6284
152	N	L	-1.6804
153	Y	L	-0.9104
154	L	L	0.0000
155	A	L	0.0000
156	W	L	0.0000
157	Y	L	0.0000
158	Q	L	-0.8596
159	Q	L	0.0000
160	K	L	-1.8411
161	P	L	-1.2815
162	G	L	-1.6945
163	K	L	-2.5591
164	A	L	-1.5536
165	P	L	0.0000
166	K	L	-1.1910
167	L	L	0.0000
168	L	L	0.0000
169	I	L	0.0000
170	Y	L	0.1367
171	A	L	-0.3318
172	A	L	0.0000
173	S	L	-0.3253
174	T	L	-0.0052
175	L	L	0.2541
176	Q	L	-0.2348
177	S	L	-0.3712
178	G	L	-0.5026
179	V	L	-0.3285
180	P	L	-0.3594
181	S	L	-0.4444
182	R	L	-0.8497
183	F	L	0.0000
184	S	L	-0.3710
185	G	L	-0.2828
186	S	L	-0.7306
187	G	L	-1.4130
188	S	L	-1.7996
189	G	L	-2.1510
190	T	L	-2.2969
191	D	L	-2.4742
192	F	L	0.0000
193	T	L	-0.7513
194	L	L	0.0000
195	T	L	-0.6628
196	I	L	0.0000
197	S	L	-1.4562
198	S	L	-1.2096
199	L	L	0.0000
200	Q	L	-0.7252
201	P	L	-1.0990
202	E	L	-1.7511
203	D	L	0.0000
204	V	L	-0.8593
205	A	L	0.0000
206	T	L	-1.1888
207	Y	L	0.0000
208	Y	L	0.0000
209	C	L	0.0000
210	Q	L	0.0000
211	R	L	0.0000
212	Y	L	0.0000
213	N	L	-1.5046
214	R	L	-2.0313
215	A	L	-1.1518
216	P	L	-1.4549
217	Y	L	0.0000
218	T	L	-1.0013
219	F	L	-0.4737
220	G	L	0.0000
221	Q	L	-1.8629
222	G	L	0.0000
223	T	L	0.0000
224	K	L	-2.0035
225	V	L	0.0000
226	E	L	-1.8024
227	I	L	-1.0419
228	K	L	-1.6008