| Chain sequence(s) |
A: MLPARCARLLTPHLLLVLVQLSPARGHRTTGPRFLISDRDPQCNLHCSRTQPKPICASDGRSYESMCEYQRAKCRDPTLGVVHRGRCKDAGQSKCRLERAQALEQAKKPQEAVFVPECGEDGSFTQVQCHTYTGYCWCVTPDGKPISGSSVQNKTPVCSGSVTDKPLSQGNSGRKDDGSKPTPTMETQPVFDGDEITAPTLWIKHLVIKDSKLNNTNIRNSEKVYSCDQERQSALEEAQQNPREGIVIPECAPGGLYKPVQCHQSTGYCWCVLVDTGRPLPGTSTRYVMPSCESDARAKTTEADDPFKDRELPGCPEGKKMEFITSLLDALTTDMVQAINSAAPTGGGRFSEPDPSHTLEERVVHWYFSQLDSNSSNDINKREMKPFKRYVKKKAKPKKCARRFTDYCDLNKDKVISLPELKGCLGVSKEGRLV
input PDB |
| Selected Chain(s) | A |
| Distance of aggregation | 10 Å |
| FoldX usage | Yes |
| Dynamic mode | No |
| Automated mutations | No |
| Mutated residues | RH278A,RH286A,AT296A,EG302A,GR85A |
| Energy difference between WT (input) and mutated protein (by FoldX) | 6.06687 kcal/mol
CAUTION: Your mutation/s can destabilize the protein structure |
| Downloads | Download all the data |
| Simulation log |
[INFO] Logger: Verbosity set to: 2 - [INFO] (00:00:09)
[WARNING] runJob: Working directory already exists (possibly overwriting previous results -ow
to prevent this behavior) (00:00:09)
[INFO] runJob: Starting aggrescan3d job on: input.pdb with A chain(s) selected (00:00:09)
[INFO] runJob: Creating pdb object from: input.pdb (00:00:09)
[INFO] FoldX: Starting FoldX energy minimalization (00:00:09)
[INFO] FoldX: Building mutant model (00:03:33)
[INFO] FoldX: Starting FoldX energy minimalization (00:04:42)
[INFO] Analysis: Starting Aggrescan3D on folded.pdb (00:07:24)
[INFO] Main: Simulation completed successfully. (00:07:27)
|
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 1 | M | A | 1.7444 | |
| 2 | L | A | 1.9255 | |
| 3 | P | A | 0.5086 | |
| 4 | A | A | -0.5895 | |
| 5 | R | A | -1.4974 | |
| 6 | C | A | -0.5203 | |
| 7 | A | A | -0.5448 | |
| 8 | R | A | -0.5617 | |
| 9 | L | A | 1.4785 | |
| 10 | L | A | 1.9147 | |
| 11 | T | A | 0.6297 | |
| 12 | P | A | -0.1161 | |
| 13 | H | A | 0.0422 | |
| 14 | L | A | 2.0011 | |
| 15 | L | A | 3.2949 | |
| 16 | L | A | 3.8656 | |
| 17 | V | A | 3.9922 | |
| 18 | L | A | 3.4102 | |
| 19 | V | A | 2.7398 | |
| 20 | Q | A | 0.6727 | |
| 21 | L | A | 1.1414 | |
| 22 | S | A | -0.0424 | |
| 23 | P | A | -0.3903 | |
| 24 | A | A | -1.2144 | |
| 25 | R | A | -2.5193 | |
| 26 | G | A | -2.2562 | |
| 27 | H | A | -2.5327 | |
| 28 | R | A | -2.7361 | |
| 29 | T | A | -1.5283 | |
| 30 | T | A | -1.0886 | |
| 31 | G | A | -0.9795 | |
| 32 | P | A | -0.6663 | |
| 33 | R | A | -0.3896 | |
| 34 | F | A | 2.3104 | |
| 35 | L | A | 2.6150 | |
| 36 | I | A | 2.2830 | |
| 37 | S | A | 0.0985 | |
| 38 | D | A | -2.6309 | |
| 39 | R | A | -3.5644 | |
| 40 | D | A | -3.8261 | |
| 41 | P | A | -2.6286 | |
| 42 | Q | A | -2.5102 | |
| 43 | C | A | 0.0000 | |
| 44 | N | A | -2.7440 | |
| 45 | L | A | -1.0893 | |
| 46 | H | A | -1.4463 | |
| 47 | C | A | -1.1454 | |
| 48 | S | A | -1.3230 | |
| 49 | R | A | -2.3368 | |
| 50 | T | A | -1.8827 | |
| 51 | Q | A | -2.1965 | |
| 52 | P | A | -2.0836 | |
| 53 | K | A | -2.7867 | |
| 54 | P | A | -3.0825 | |
| 55 | I | A | 0.0000 | |
| 56 | C | A | -2.4566 | |
| 57 | A | A | 0.0000 | |
| 58 | S | A | -0.7271 | |
| 59 | D | A | -1.9166 | |
| 60 | G | A | -2.5612 | |
| 61 | R | A | -3.1151 | |
| 62 | S | A | -3.0107 | |
| 63 | Y | A | -2.0105 | |
| 64 | E | A | -2.3134 | |
| 65 | S | A | 0.0000 | |
| 66 | M | A | -0.4608 | |
| 67 | C | A | -1.0479 | |
| 68 | E | A | -1.1592 | |
| 69 | Y | A | 0.0000 | |
| 70 | Q | A | -1.4150 | |
| 71 | R | A | -2.0296 | |
| 72 | A | A | -1.7167 | |
| 73 | K | A | -1.9792 | |
| 74 | C | A | -1.8113 | |
| 75 | R | A | -2.8343 | |
| 76 | D | A | -2.1712 | |
| 77 | P | A | -1.3238 | |
| 78 | T | A | -0.9300 | |
| 79 | L | A | 0.0000 | |
| 80 | G | A | -0.0384 | |
| 81 | V | A | 0.3015 | |
| 82 | V | A | 0.7520 | |
| 83 | H | A | -1.6446 | |
| 84 | R | A | -3.3462 | |
| 85 | R | A | -4.3152 | mutated: GR85A |
| 86 | R | A | -4.6032 | |
| 87 | C | A | -3.4866 | |
| 88 | K | A | -3.6404 | |
| 89 | D | A | -3.5881 | |
| 90 | A | A | -2.4943 | |
| 91 | G | A | -2.0799 | |
| 92 | Q | A | -2.6895 | |
| 93 | S | A | -3.0257 | |
| 94 | K | A | -3.3199 | |
| 95 | C | A | 0.0000 | |
| 96 | R | A | -2.8716 | |
| 97 | L | A | -2.2431 | |
| 98 | E | A | -2.9281 | |
| 99 | R | A | -2.1593 | |
| 100 | A | A | -1.6397 | |
| 101 | Q | A | -2.3127 | |
| 102 | A | A | 0.0000 | |
| 103 | L | A | -0.9983 | |
| 104 | E | A | -3.0237 | |
| 105 | Q | A | -2.8451 | |
| 106 | A | A | -2.3203 | |
| 107 | K | A | -3.4343 | |
| 108 | K | A | -3.6962 | |
| 109 | P | A | -2.6139 | |
| 110 | Q | A | -3.0203 | |
| 111 | E | A | -3.3644 | |
| 112 | A | A | -1.2480 | |
| 113 | V | A | -0.6183 | |
| 114 | F | A | 0.8390 | |
| 115 | V | A | 0.0511 | |
| 116 | P | A | 0.0000 | |
| 117 | E | A | -1.3440 | |
| 118 | C | A | -1.9758 | |
| 119 | G | A | -2.0266 | |
| 120 | E | A | -2.8085 | |
| 121 | D | A | -2.6814 | |
| 122 | G | A | 0.0000 | |
| 123 | S | A | -2.2001 | |
| 124 | F | A | -2.0522 | |
| 125 | T | A | -1.2353 | |
| 126 | Q | A | -1.3616 | |
| 127 | V | A | 0.0503 | |
| 128 | Q | A | 0.0000 | |
| 129 | C | A | 0.3136 | |
| 130 | H | A | 0.7572 | |
| 131 | T | A | 0.8150 | |
| 132 | Y | A | 1.3455 | |
| 133 | T | A | 0.2700 | |
| 134 | G | A | -0.3909 | |
| 135 | Y | A | -0.1348 | |
| 136 | C | A | -0.2535 | |
| 137 | W | A | 0.3200 | |
| 138 | C | A | 0.0000 | |
| 139 | V | A | 0.0000 | |
| 140 | T | A | -1.4219 | |
| 141 | P | A | -1.7070 | |
| 142 | D | A | -2.9623 | |
| 143 | G | A | -2.3907 | |
| 144 | K | A | -2.2951 | |
| 145 | P | A | -0.9611 | |
| 146 | I | A | 0.2565 | |
| 147 | S | A | -0.2879 | |
| 148 | G | A | -0.0993 | |
| 149 | S | A | 0.4447 | |
| 150 | S | A | -0.1077 | |
| 151 | V | A | -0.5987 | |
| 152 | Q | A | -1.8645 | |
| 153 | N | A | -2.3988 | |
| 154 | K | A | -2.2320 | |
| 155 | T | A | -0.5979 | |
| 156 | P | A | 0.0000 | |
| 157 | V | A | 1.5064 | |
| 158 | C | A | 0.7490 | |
| 159 | S | A | -0.2092 | |
| 160 | G | A | -0.4088 | |
| 161 | S | A | 0.1324 | |
| 162 | V | A | 0.9213 | |
| 163 | T | A | -0.6476 | |
| 164 | D | A | -2.2424 | |
| 165 | K | A | -2.1881 | |
| 166 | P | A | -0.9996 | |
| 167 | L | A | 0.2779 | |
| 168 | S | A | -0.5950 | |
| 169 | Q | A | -1.4573 | |
| 170 | G | A | -1.6688 | |
| 171 | N | A | -2.0322 | |
| 172 | S | A | -1.6791 | |
| 173 | G | A | -2.1592 | |
| 174 | R | A | -3.4006 | |
| 175 | K | A | -4.1097 | |
| 176 | D | A | -4.0979 | |
| 177 | D | A | -3.3007 | |
| 178 | G | A | -2.3410 | |
| 179 | S | A | -1.7997 | |
| 180 | K | A | -2.0534 | |
| 181 | P | A | -1.1994 | |
| 182 | T | A | -0.6754 | |
| 183 | P | A | -0.1504 | |
| 184 | T | A | -0.0524 | |
| 185 | M | A | 0.0570 | |
| 186 | E | A | -1.6770 | |
| 187 | T | A | -1.1655 | |
| 188 | Q | A | -1.3672 | |
| 189 | P | A | 0.2744 | |
| 190 | V | A | 1.9339 | |
| 191 | F | A | 1.7726 | |
| 192 | D | A | -1.5358 | |
| 193 | G | A | -2.6662 | |
| 194 | D | A | -3.5692 | |
| 195 | E | A | -2.7411 | |
| 196 | I | A | -0.3364 | |
| 197 | T | A | -0.2280 | |
| 198 | A | A | -0.0943 | |
| 199 | P | A | 0.0090 | |
| 200 | T | A | 0.3114 | |
| 201 | L | A | 0.5755 | |
| 202 | W | A | 1.1126 | |
| 203 | I | A | 2.0536 | |
| 204 | K | A | 0.7931 | |
| 205 | H | A | 0.8084 | |
| 206 | L | A | 1.6016 | |
| 207 | V | A | 1.8311 | |
| 208 | I | A | 1.2551 | |
| 209 | K | A | -1.0231 | |
| 210 | D | A | -1.6769 | |
| 211 | S | A | -1.2413 | |
| 212 | K | A | -2.1056 | |
| 213 | L | A | -0.9860 | |
| 214 | N | A | -2.3583 | |
| 215 | N | A | -2.1419 | |
| 216 | T | A | -1.1709 | |
| 217 | N | A | -1.1798 | |
| 218 | I | A | 0.0342 | |
| 219 | R | A | -1.9948 | |
| 220 | N | A | -2.5540 | |
| 221 | S | A | -2.1088 | |
| 222 | E | A | -2.5955 | |
| 223 | K | A | -1.6310 | |
| 224 | V | A | 0.9648 | |
| 225 | Y | A | 0.7864 | |
| 226 | S | A | -0.2593 | |
| 227 | C | A | 0.0000 | |
| 228 | D | A | -2.8350 | |
| 229 | Q | A | -2.2502 | |
| 230 | E | A | -2.0616 | |
| 231 | R | A | -2.5724 | |
| 232 | Q | A | -2.7134 | |
| 233 | S | A | -2.2257 | |
| 234 | A | A | 0.0000 | |
| 235 | L | A | -1.5762 | |
| 236 | E | A | -3.0576 | |
| 237 | E | A | -2.8543 | |
| 238 | A | A | -2.6016 | |
| 239 | Q | A | -2.9128 | |
| 240 | Q | A | -3.1903 | |
| 241 | N | A | -3.1333 | |
| 242 | P | A | -2.6444 | |
| 243 | R | A | -3.5205 | |
| 244 | E | A | -3.4031 | |
| 245 | G | A | -1.6369 | |
| 246 | I | A | -1.2804 | |
| 247 | V | A | 0.0000 | |
| 248 | I | A | -0.3811 | |
| 249 | P | A | 0.0000 | |
| 250 | E | A | -1.2763 | |
| 251 | C | A | -1.0994 | |
| 252 | A | A | -0.5871 | |
| 253 | P | A | -0.5797 | |
| 254 | G | A | -0.6275 | |
| 255 | G | A | -0.9065 | |
| 256 | L | A | 0.0792 | |
| 257 | Y | A | 0.0000 | |
| 258 | K | A | -0.8830 | |
| 259 | P | A | -1.2165 | |
| 260 | V | A | -0.1814 | |
| 261 | Q | A | 0.0000 | |
| 262 | C | A | 0.2594 | |
| 263 | H | A | 0.0000 | |
| 264 | Q | A | -0.9949 | |
| 265 | S | A | -0.9388 | |
| 266 | T | A | -0.1594 | |
| 267 | G | A | 0.2967 | |
| 268 | Y | A | 0.5718 | |
| 269 | C | A | 0.0000 | |
| 270 | W | A | 0.0000 | |
| 271 | C | A | 0.0000 | |
| 272 | V | A | 0.0000 | |
| 273 | L | A | -1.5104 | |
| 274 | V | A | -1.1887 | |
| 275 | D | A | -0.5475 | |
| 276 | T | A | -0.0127 | |
| 277 | G | A | -0.4535 | |
| 278 | H | A | -1.0507 | mutated: RH278A |
| 279 | P | A | -0.4800 | |
| 280 | L | A | -0.2259 | |
| 281 | P | A | -0.4865 | |
| 282 | G | A | -0.7754 | |
| 283 | T | A | -0.2509 | |
| 284 | S | A | -0.1086 | |
| 285 | T | A | 0.5980 | |
| 286 | H | A | 0.7600 | mutated: RH286A |
| 287 | Y | A | 2.0015 | |
| 288 | V | A | 2.6925 | |
| 289 | M | A | 2.1136 | |
| 290 | P | A | 0.7625 | |
| 291 | S | A | -0.1264 | |
| 292 | C | A | -1.0146 | |
| 293 | E | A | -2.6355 | |
| 294 | S | A | -2.2469 | |
| 295 | D | A | -3.0267 | |
| 296 | T | A | -2.5553 | mutated: AT296A |
| 297 | R | A | -3.1867 | |
| 298 | A | A | -2.0610 | |
| 299 | K | A | -2.3299 | |
| 300 | T | A | -1.6453 | |
| 301 | T | A | -1.8221 | |
| 302 | G | A | -2.3351 | mutated: EG302A |
| 303 | A | A | -1.8193 | |
| 304 | D | A | -2.8872 | |
| 305 | D | A | -3.1062 | |
| 306 | P | A | -2.1923 | |
| 307 | F | A | 0.0000 | |
| 308 | K | A | -3.7551 | |
| 309 | D | A | -3.8742 | |
| 310 | R | A | -3.2862 | |
| 311 | E | A | -3.3837 | |
| 312 | L | A | 0.0000 | |
| 313 | P | A | -1.9143 | |
| 314 | G | A | -1.8301 | |
| 315 | C | A | -2.4667 | |
| 316 | P | A | -2.0466 | |
| 317 | E | A | -2.6870 | |
| 318 | G | A | -2.2617 | |
| 319 | K | A | -2.7819 | |
| 320 | K | A | -2.0332 | |
| 321 | M | A | -0.8748 | |
| 322 | E | A | -2.2268 | |
| 323 | F | A | 0.0000 | |
| 324 | I | A | 0.0000 | |
| 325 | T | A | -1.1544 | |
| 326 | S | A | -1.5606 | |
| 327 | L | A | 0.0000 | |
| 328 | L | A | -1.1037 | |
| 329 | D | A | -2.0255 | |
| 330 | A | A | -1.1209 | |
| 331 | L | A | 0.0000 | |
| 332 | T | A | -0.6303 | |
| 333 | T | A | -0.5234 | |
| 334 | D | A | -0.7138 | |
| 335 | M | A | 0.0000 | |
| 336 | V | A | 0.4686 | |
| 337 | Q | A | -0.9710 | |
| 338 | A | A | -0.2368 | |
| 339 | I | A | 0.0506 | |
| 340 | N | A | -1.1001 | |
| 341 | S | A | -0.7031 | |
| 342 | A | A | -0.6584 | |
| 343 | A | A | -0.4494 | |
| 344 | P | A | -0.6340 | |
| 345 | T | A | -0.5466 | |
| 346 | G | A | -1.1188 | |
| 347 | G | A | -1.3182 | |
| 348 | G | A | -1.5271 | |
| 349 | R | A | -2.1606 | |
| 350 | F | A | -0.8089 | |
| 351 | S | A | -1.5609 | |
| 352 | E | A | -2.3910 | |
| 353 | P | A | -1.8669 | |
| 354 | D | A | -2.5549 | |
| 355 | P | A | -1.5012 | |
| 356 | S | A | -1.0477 | |
| 357 | H | A | -1.3679 | |
| 358 | T | A | -0.6703 | |
| 359 | L | A | 0.2891 | |
| 360 | E | A | -1.0340 | |
| 361 | E | A | 0.0000 | |
| 362 | R | A | -1.1833 | |
| 363 | V | A | 0.0000 | |
| 364 | V | A | 0.0000 | |
| 365 | H | A | -1.0185 | |
| 366 | W | A | -0.8197 | |
| 367 | Y | A | 0.0000 | |
| 368 | F | A | 0.0000 | |
| 369 | S | A | -1.3693 | |
| 370 | Q | A | -1.4423 | |
| 371 | L | A | 0.0000 | |
| 372 | D | A | -1.9237 | |
| 373 | S | A | -1.7836 | |
| 374 | N | A | -1.9334 | |
| 375 | S | A | -1.6874 | |
| 376 | S | A | -1.6376 | |
| 377 | N | A | -2.0232 | |
| 378 | D | A | -1.8409 | |
| 379 | I | A | 0.0000 | |
| 380 | N | A | -2.9642 | |
| 381 | K | A | -3.4686 | |
| 382 | R | A | -3.2248 | |
| 383 | E | A | 0.0000 | |
| 384 | M | A | 0.0000 | |
| 385 | K | A | -3.1474 | |
| 386 | P | A | -2.1203 | |
| 387 | F | A | 0.0000 | |
| 388 | K | A | -3.3054 | |
| 389 | R | A | -3.6959 | |
| 390 | Y | A | -2.5441 | |
| 391 | V | A | 0.0000 | |
| 392 | K | A | -4.7584 | |
| 393 | K | A | -3.8627 | |
| 394 | K | A | -2.8908 | |
| 395 | A | A | 0.0000 | |
| 396 | K | A | -3.7991 | |
| 397 | P | A | -3.2573 | |
| 398 | K | A | -4.2628 | |
| 399 | K | A | -3.8905 | |
| 400 | C | A | 0.0000 | |
| 401 | A | A | 0.0000 | |
| 402 | R | A | -3.8124 | |
| 403 | R | A | -2.7060 | |
| 404 | F | A | 0.0000 | |
| 405 | T | A | 0.0000 | |
| 406 | D | A | -2.5031 | |
| 407 | Y | A | 0.0000 | |
| 408 | C | A | 0.0000 | |
| 409 | D | A | -2.0696 | |
| 410 | L | A | -1.6460 | |
| 411 | N | A | -2.8619 | |
| 412 | K | A | -3.7300 | |
| 413 | D | A | -3.6972 | |
| 414 | K | A | -3.7478 | |
| 415 | V | A | -2.6288 | |
| 416 | I | A | 0.0000 | |
| 417 | S | A | -0.9631 | |
| 418 | L | A | -0.5081 | |
| 419 | P | A | -0.6023 | |
| 420 | E | A | -0.4995 | |
| 421 | L | A | 0.0000 | |
| 422 | K | A | 0.0000 | |
| 423 | G | A | -1.0777 | |
| 424 | C | A | 0.0000 | |
| 425 | L | A | 0.0000 | |
| 426 | G | A | -1.1552 | |
| 427 | V | A | 0.0000 | |
| 428 | S | A | -2.4066 | |
| 429 | K | A | -3.3115 | |
| 430 | E | A | -3.2829 | |
| 431 | G | A | -2.2480 | |
| 432 | R | A | -1.6117 | |
| 433 | L | A | 1.0854 | |
| 434 | V | A | 1.9700 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine