Project name: 2FB4

Chain sequence(s)	H: EVQLVQSGGGVVQPGRSLRLSCSSSGFIFSSYAMYWVRQAPGKGLEWVAIIWDDGSDQHYADSVKGRFTISRNDSKNTLFLQMDSLRPEDTGVYFCARDGGHGFCSSASCFGPDYWGQGTPVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPQPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC; L: QSVLTQPPSASGTPGQRVTISCSGTSSNIGSSTVNWYQQLPGMAPKLLIYRDAMRPSGVPDRFSGSKSGASASLAIGGLQSEDETDYYCAAWDVSLNAYVFGTGTKVTVLGQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS
Selected Chain(s)	H,L
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:03:33)
[INFO]       Main:     Simulation completed successfully.                                          (00:03:36)

• Minimal score value: -3.3019
• Maximal score value: 1.8246
• Average score: -0.7149
• Total score value: -318.1256

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	Q	L	-1.0702
2	S	L	-0.3478
3	V	L	0.8329
4	L	L	0.0000
5	T	L	0.0357
6	Q	L	0.0000
7	P	L	-0.5014
8	P	L	-0.6815
9	S	L	-0.7585
10	A	L	-0.4869
11	S	L	-0.5346
12	G	L	0.0000
13	T	L	-0.8593
14	P	L	-1.2633
15	G	L	-1.7535
16	Q	L	-2.2938
17	R	L	-2.6855
18	V	L	0.0000
19	T	L	-0.7355
20	I	L	0.0000
21	S	L	-0.4544
22	C	L	0.0000
23	S	L	-0.3285
24	G	L	-0.4084
25	T	L	-0.4721
26	S	L	-0.5343
27	S	L	-0.3595
27A	N	L	0.0000
27B	I	L	0.0000
28	G	L	-0.8174
29	S	L	-0.4161
30	S	L	-0.4088
31	T	L	-0.7394
32	V	L	0.0000
33	N	L	0.0000
34	W	L	0.0000
35	Y	L	0.0000
36	Q	L	-0.6936
37	Q	L	0.0000
38	L	L	0.7085
39	P	L	0.0402
40	G	L	-0.0491
41	M	L	0.7303
42	A	L	-0.0398
43	P	L	0.0000
44	K	L	-1.3620
45	L	L	0.0000
46	L	L	0.0000
47	I	L	0.0000
48	Y	L	-0.6014
49	R	L	-1.3940
50	D	L	-1.1700
51	A	L	-0.6123
52	M	L	-0.3135
53	R	L	-1.3826
54	P	L	-0.6449
55	S	L	-0.8685
56	G	L	-0.9976
57	V	L	0.0000
58	P	L	-1.4263
59	D	L	-2.2941
60	R	L	-1.6376
61	F	L	0.0000
62	S	L	-0.8389
63	G	L	-0.6366
64	S	L	-0.8166
65	K	L	-1.2560
66	S	L	-0.9200
67	G	L	-0.9557
68	A	L	-0.7959
69	S	L	-0.8183
70	A	L	0.0000
71	S	L	-0.5399
72	L	L	0.0000
73	A	L	-0.6801
74	I	L	0.0000
75	G	L	-1.8343
76	G	L	-1.7018
77	L	L	0.0000
78	Q	L	-2.1092
79	S	L	-1.6160
80	E	L	-2.3192
81	D	L	0.0000
82	E	L	-1.2990
83	T	L	-0.7097
84	D	L	-0.5537
85	Y	L	0.0000
86	Y	L	0.0000
87	C	L	0.0000
88	A	L	0.0000
89	A	L	0.0000
90	W	L	0.0000
91	D	L	0.0000
92	V	L	0.2130
93	S	L	-0.0261
94	L	L	0.0568
95	N	L	-0.4253
96	A	L	-0.4190
97	Y	L	0.0000
98	V	L	0.0000
99	F	L	0.0000
100	G	L	0.0000
101	T	L	-0.2583
102	G	L	-0.7296
103	T	L	0.0000
104	K	L	-1.1252
105	V	L	0.0000
106	T	L	-0.4737
107	V	L	0.0000
108	L	L	0.1419
109	G	L	-0.7817
110	Q	L	-1.3388
111	P	L	-1.5504
112	K	L	-2.6717
113	A	L	-2.0436
114	N	L	-2.1263
115	P	L	0.0000
116	T	L	-0.5300
117	V	L	0.0000
118	T	L	-0.3058
119	L	L	-0.2738
120	F	L	0.0000
121	P	L	-0.1489
122	P	L	0.0000
123	S	L	0.0000
124	S	L	-1.4882
125	E	L	-2.0699
126	E	L	0.0000
127	L	L	-1.9710
128	Q	L	-2.3028
129	A	L	-1.8857
130	N	L	-2.7254
131	K	L	-2.6454
132	A	L	0.0000
133	T	L	0.0000
134	L	L	0.0000
135	V	L	0.0000
136	C	L	0.0000
137	L	L	0.0000
138	I	L	0.0000
139	S	L	-0.9263
140	D	L	-1.8496
141	F	L	0.0000
142	Y	L	-1.8863
143	P	L	-1.5692
144	G	L	-1.2110
145	A	L	-0.3882
146	V	L	-0.2589
147	T	L	-0.0575
148	V	L	0.1196
149	A	L	-0.3756
150	W	L	0.0000
151	K	L	-1.4231
152	A	L	0.0000
153	D	L	-1.9832
154	G	L	-1.3981
155	S	L	-1.1408
156	P	L	-1.0719
157	V	L	-0.9280
158	K	L	-1.6136
159	A	L	-0.7315
160	G	L	-0.3779
161	V	L	0.0947
162	E	L	-0.2111
163	T	L	-0.2764
164	T	L	-0.7643
165	K	L	-1.9161
166	P	L	-1.3389
167	S	L	-1.6546
168	K	L	-2.8022
169	Q	L	-2.2524
170	S	L	-1.6702
171	N	L	-2.2928
172	N	L	-2.7468
173	K	L	-2.5629
174	Y	L	-1.8249
175	A	L	0.0000
176	A	L	0.0000
177	S	L	0.0000
178	S	L	0.0000
179	Y	L	0.0000
180	L	L	0.0000
181	S	L	-0.5645
182	L	L	0.0000
183	T	L	-2.0197
184	P	L	-2.5734
185	E	L	-3.3019
186	Q	L	-2.5904
187	W	L	0.0000
188	K	L	-3.0846
189	S	L	-2.5104
190	H	L	-2.5558
191	R	L	-2.6413
192	S	L	-1.7047
193	Y	L	0.0000
194	S	L	0.0000
195	C	L	0.0000
196	Q	L	-1.2156
197	V	L	0.0000
198	T	L	-0.8929
199	H	L	0.0000
200	E	L	-2.5076
201	G	L	-1.4208
202	S	L	-1.0975
203	T	L	-0.9773
204	V	L	-1.0240
205	E	L	-2.1846
206	K	L	-1.5513
207	T	L	-0.8954
208	V	L	-0.5667
209	A	L	-1.0856
210	P	L	-1.2105
211	T	L	-0.9476
212	E	L	-1.1617
213	C	L	0.0420
214	S	L	-0.1958
1	E	H	-2.1265
2	V	H	-1.1189
3	Q	H	-1.4125
4	L	H	0.0000
5	V	H	0.7822
6	Q	H	0.0000
7	S	H	-0.5221
8	G	H	-1.1144
9	G	H	-0.6299
10	G	H	0.0160
11	V	H	0.7469
12	V	H	-0.2880
13	Q	H	-1.1457
14	P	H	-2.0159
15	G	H	-2.1916
16	R	H	-2.5596
17	S	H	-2.0378
18	L	H	-1.3161
19	R	H	-2.0008
20	L	H	0.0000
21	S	H	-0.3768
22	C	H	0.0000
23	S	H	-0.3781
24	S	H	-0.4049
25	S	H	-0.6634
26	G	H	-0.9277
27	F	H	0.4374
28	I	H	1.8246
29	F	H	0.0000
30	S	H	-0.7094
31	S	H	-0.0848
32	Y	H	0.0583
33	A	H	0.0000
34	M	H	0.0000
35	Y	H	0.0000
36	W	H	0.0000
37	V	H	0.0000
38	R	H	0.0000
39	Q	H	0.0000
40	A	H	-1.3278
41	P	H	-1.1032
42	G	H	-1.5240
43	K	H	-2.2805
44	G	H	-1.4618
45	L	H	0.0000
46	E	H	-1.1205
47	W	H	0.0000
48	V	H	0.0000
49	A	H	0.0000
50	I	H	0.0000
51	I	H	0.0000
52	W	H	-0.8749
53	D	H	-1.5093
54	D	H	-2.3391
55	G	H	-1.6748
56	S	H	-1.4427
57	D	H	-1.4578
58	Q	H	-0.9346
59	H	H	-0.9734
60	Y	H	-1.1249
61	A	H	-1.5231
62	D	H	-2.5148
63	S	H	-1.8221
64	V	H	0.0000
65	K	H	-2.6342
66	G	H	-1.8235
67	R	H	-1.6171
68	F	H	0.0000
69	T	H	-0.8783
70	I	H	0.0000
71	S	H	-0.6645
72	R	H	-1.2813
73	N	H	-1.8890
74	D	H	-2.0523
75	S	H	-1.7754
76	K	H	-2.5416
77	N	H	-1.7165
78	T	H	-1.1344
79	L	H	0.0000
80	F	H	-0.3646
81	L	H	0.0000
82	Q	H	-1.0693
83	M	H	0.0000
84	D	H	-2.0978
85	S	H	-1.9992
86	L	H	0.0000
87	R	H	-3.0795
88	P	H	-2.0262
89	E	H	-2.5017
90	D	H	0.0000
91	T	H	-0.8520
92	G	H	0.0000
93	V	H	0.0047
94	Y	H	0.0000
95	F	H	0.0000
96	C	H	0.0000
97	A	H	0.0000
98	R	H	0.0000
99	D	H	0.0000
100	G	H	-0.0000
101	G	H	-0.2872
101A	H	H	-0.7054
101B	G	H	0.0000
101C	F	H	0.5020
101D	C	H	0.0000
102	S	H	0.0776
103	S	H	-0.2589
104	A	H	0.0122
104A	S	H	-0.2450
104B	C	H	0.0000
104C	F	H	-0.1519
104D	G	H	0.0000
105	P	H	0.0000
106	D	H	-0.0948
107	Y	H	-0.0580
108	W	H	-0.1726
109	G	H	0.0000
110	Q	H	-0.7372
111	G	H	0.0000
112	T	H	0.0000
113	P	H	-0.3403
114	V	H	0.0000
115	T	H	-0.1911
116	V	H	0.0000
117	S	H	-0.6880
118	S	H	-0.6929
119	A	H	-0.4175
120	S	H	-0.5874
121	T	H	-0.8441
122	K	H	-1.8375
123	G	H	-1.7592
124	P	H	0.0000
125	S	H	-0.4492
126	V	H	0.0000
127	F	H	-0.6883
128	P	H	-1.0917
129	L	H	0.0000
130	A	H	-0.9658
131	P	H	0.0000
132	S	H	-1.2705
133	S	H	-1.2694
134	K	H	-1.9653
135	S	H	-1.3736
136	T	H	-0.9460
137	S	H	-0.6114
138	G	H	-0.9093
139	G	H	-0.9689
140	T	H	-0.6518
141	A	H	0.0000
142	A	H	-0.0780
143	L	H	0.0000
144	G	H	0.0000
145	C	H	0.0000
146	L	H	0.0000
147	V	H	0.0000
148	K	H	-0.3765
149	D	H	-0.6399
150	Y	H	0.0000
151	F	H	0.0000
152	P	H	0.0000
153	Q	H	-0.5758
154	P	H	-0.8043
155	V	H	-0.6477
156	T	H	-0.5186
157	V	H	-0.3127
158	S	H	-0.3310
159	W	H	0.0000
160	N	H	-0.8114
161	S	H	-0.6619
162	G	H	-0.4373
163	A	H	-0.2091
164	L	H	-0.0194
165	T	H	-0.1990
166	S	H	-0.3121
167	G	H	-0.4092
168	V	H	0.0749
169	H	H	-0.2040
170	T	H	-0.0824
171	F	H	0.0000
172	P	H	-0.1561
173	A	H	0.2607
174	V	H	0.0000
175	L	H	1.2665
176	Q	H	0.3586
177	S	H	-0.1034
178	S	H	-0.1798
179	G	H	0.0267
180	L	H	-0.0491
181	Y	H	0.2677
182	S	H	0.0000
183	L	H	0.0000
184	S	H	0.0000
185	S	H	0.0000
186	V	H	0.0000
187	V	H	0.0000
188	T	H	-0.2931
189	V	H	0.0000
190	P	H	-0.6728
191	S	H	-0.6942
192	S	H	-0.6395
193	S	H	-0.6701
194	L	H	-0.8787
195	G	H	-1.2062
196	T	H	-0.8824
197	Q	H	-1.5796
198	T	H	-1.3474
199	Y	H	0.0000
200	I	H	-1.4532
201	C	H	0.0000
202	N	H	-1.5453
203	V	H	0.0000
204	N	H	-1.8167
205	H	H	0.0000
206	K	H	-2.7428
207	P	H	-1.6784
208	S	H	-1.9987
209	N	H	-2.6631
210	T	H	-2.1258
211	K	H	-2.6531
212	V	H	-1.8807
213	D	H	-2.6693
214	K	H	-2.1902
215	R	H	-2.7865
216	V	H	0.0000
217	E	H	-2.7167
218	P	H	-1.3059
219	K	H	-1.1247
220	S	H	-0.4630
221	C	H	0.3028