Project name: ELK-GFP

Chain sequence(s)	A: LELELKLKLELELKLKENLYFQGRGSGSESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEE
Selected Chain(s)	A
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:01)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:01)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with A chain(s) selected             (00:00:01)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:01)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:01)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:04:05)
[INFO]       Main:     Simulation completed successfully.                                          (00:04:07)

• Minimal score value: -3.3571
• Maximal score value: 1.0179
• Average score: -0.8312
• Total score value: -215.2896

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	L	A	0.7086
2	E	A	-0.7349
3	L	A	0.5356
4	E	A	-0.7168
5	L	A	0.4260
6	K	A	-0.8027
7	L	A	0.3055
8	K	A	-0.7578
9	L	A	0.5330
10	E	A	-0.7982
11	L	A	0.2311
12	E	A	-0.8787
13	L	A	0.1694
14	K	A	-1.2667
15	L	A	0.2391
16	K	A	-0.5257
17	E	A	-0.6331
18	N	A	-0.2974
19	L	A	0.9022
20	Y	A	1.0161
21	F	A	0.1606
22	Q	A	-1.2898
23	G	A	-1.0872
24	R	A	-2.2847
25	G	A	-2.0051
26	S	A	-1.2708
27	G	A	-1.4854
28	S	A	-1.7559
29	E	A	-2.7172
30	S	A	-2.5881
31	D	A	-3.3571
32	E	A	-2.9957
33	S	A	-1.6502
34	G	A	-1.2710
35	L	A	0.3484
36	P	A	-0.1814
37	A	A	-1.2828
38	M	A	-1.3992
39	E	A	-2.9858
40	I	A	0.0000
41	E	A	-2.4856
42	C	A	0.0000
43	R	A	-2.2625
44	I	A	0.0000
45	T	A	-1.3295
46	G	A	0.0000
47	T	A	-0.8213
48	L	A	0.0000
49	N	A	-1.2316
50	G	A	-0.7277
51	V	A	-0.1816
52	E	A	-1.8048
53	F	A	0.0000
54	E	A	-1.6885
55	L	A	0.0000
56	V	A	-1.2440
57	G	A	-1.6479
58	G	A	-2.0981
59	G	A	-2.2287
60	E	A	-3.0630
61	G	A	0.0000
62	T	A	-1.7344
63	P	A	0.0000
64	E	A	-2.4658
65	Q	A	-2.6597
66	G	A	0.0000
67	R	A	-2.0858
68	M	A	0.0000
69	T	A	-1.4619
70	N	A	0.0000
71	K	A	-2.9745
72	M	A	0.0000
73	K	A	-3.1635
74	S	A	0.0000
75	T	A	-1.5015
76	K	A	-2.5162
77	G	A	-1.6326
78	A	A	-1.3417
79	L	A	0.0000
80	T	A	-0.1488
81	F	A	0.0000
82	S	A	0.0000
83	P	A	-0.8405
84	Y	A	-0.3410
85	L	A	0.0000
86	L	A	0.0000
87	S	A	0.0000
88	H	A	0.0000
89	V	A	0.0000
90	M	A	0.0000
91	G	A	0.0000
92	Y	A	0.0000
93	G	A	0.0000
94	F	A	0.0000
95	Y	A	0.0000
96	H	A	0.0000
97	F	A	0.0000
98	G	A	0.0000
99	T	A	-0.9517
100	Y	A	-0.2332
101	P	A	-0.1718
102	S	A	-0.4236
103	G	A	-0.6729
104	Y	A	-0.6297
105	E	A	-1.0583
106	N	A	0.0000
107	P	A	0.0000
108	F	A	0.0000
109	L	A	-0.0963
110	H	A	-0.6811
111	A	A	0.0000
112	I	A	-0.7604
113	N	A	-1.4292
114	N	A	-1.7372
115	G	A	-1.7489
116	G	A	0.0000
117	Y	A	0.0000
118	T	A	-0.5915
119	N	A	0.0000
120	T	A	-0.7356
121	R	A	0.0000
122	I	A	0.0442
123	E	A	0.0000
124	K	A	-1.2233
125	Y	A	0.0000
126	E	A	-2.2219
127	D	A	-2.1042
128	G	A	-1.6782
129	G	A	0.0000
130	V	A	-0.5000
131	L	A	0.0000
132	H	A	-0.4085
133	V	A	0.0000
134	S	A	-0.8467
135	F	A	0.0000
136	S	A	-1.1208
137	Y	A	-1.0700
138	R	A	-2.3959
139	Y	A	-1.8775
140	E	A	-2.4737
141	A	A	-1.3327
142	G	A	-1.5516
143	R	A	-2.6853
144	V	A	0.0000
145	I	A	-2.0355
146	G	A	0.0000
147	D	A	-2.4809
148	F	A	0.0000
149	K	A	-1.9631
150	V	A	0.0000
151	M	A	-0.1175
152	G	A	0.0000
153	T	A	-0.4853
154	G	A	-1.2072
155	F	A	0.0000
156	P	A	-1.9220
157	E	A	-2.7592
158	D	A	-2.6074
159	S	A	0.0000
160	V	A	-0.8716
161	I	A	0.0000
162	F	A	-0.5947
163	T	A	-1.0769
164	D	A	-2.1112
165	K	A	-2.2759
166	I	A	0.0000
167	I	A	-0.8847
168	R	A	-1.6241
169	S	A	0.0000
170	N	A	-0.4809
171	A	A	-0.3199
172	T	A	0.2928
173	V	A	1.0179
174	E	A	0.0000
175	H	A	-0.2373
176	L	A	0.0000
177	H	A	-0.5081
178	P	A	-0.5445
179	M	A	0.1210
180	G	A	-0.8789
181	D	A	-2.1327
182	N	A	-1.7321
183	D	A	-1.3487
184	L	A	0.0000
185	D	A	-1.1262
186	G	A	0.0000
187	S	A	-0.4580
188	F	A	0.0000
189	T	A	0.3811
190	R	A	0.0000
191	T	A	0.3104
192	F	A	0.0000
193	S	A	-0.5432
194	L	A	-1.5178
195	R	A	-3.0900
196	D	A	-2.7712
197	G	A	-1.6357
198	G	A	-0.7141
199	Y	A	0.5775
200	Y	A	0.0000
201	S	A	-0.1716
202	S	A	0.0000
203	V	A	0.8034
204	V	A	0.0000
205	D	A	-1.4639
206	S	A	0.0000
207	H	A	-1.1881
208	M	A	0.0000
209	H	A	-1.3042
210	F	A	0.0000
211	K	A	-2.3999
212	S	A	-1.8508
213	A	A	-1.4519
214	I	A	0.0000
215	H	A	-0.9976
216	P	A	-0.9264
217	S	A	-0.8282
218	I	A	0.0000
219	L	A	-0.8614
220	Q	A	-1.3004
221	N	A	-1.0069
222	G	A	-0.9594
223	G	A	-0.7305
224	P	A	-0.3919
225	M	A	0.0000
226	F	A	0.7409
227	A	A	0.0000
228	F	A	0.0000
229	R	A	0.0000
230	R	A	-0.7278
231	V	A	0.0000
232	E	A	-2.2150
233	E	A	-2.2428
234	D	A	-2.9540
235	H	A	-2.2154
236	S	A	-1.7663
237	N	A	-1.5884
238	T	A	-2.1504
239	E	A	-3.3390
240	L	A	0.0000
241	G	A	0.0000
242	I	A	0.0000
243	V	A	-1.2131
244	E	A	0.0000
245	Y	A	-0.7877
246	Q	A	0.0000
247	H	A	-0.1410
248	A	A	0.5450
249	F	A	0.7117
250	K	A	-0.3548
251	T	A	-0.6440
252	P	A	-1.4414
253	D	A	-2.4932
254	A	A	-1.8231
255	D	A	-2.7464
256	A	A	-2.0964
257	G	A	-2.4334
258	E	A	-3.2036
259	E	A	-2.8305