Project name: balance

Chain sequence(s)	A: MKLKALGGKIIFELEEPVKPEKGGAIVVGDPGEESQYGRVVKVGKGRRETVQGEVPLSIKEGDKVVIKLDKKVIEIEVNGKKYYICDESEILAIVEE; C: MKLKALGGKIIFELEEPVKPEKGGAIVVGDPGEESQYGRVVKVGKGRRETVQGEVPLSIKEGDKVVIKLDKKVIEIEVNGKKYYICDESEILAIVEE; B: MKLKALGGKIIFELEEPVKPEKGGAIVVGDPGEESQYGRVVKVGKGRRETVQGEVPLSIKEGDKVVIKLDKKVIEIEVNGKKYYICDESEILAIVEE; E: MKLKALGGKIIFELEEPVKPEKGGAIVVGDPGEESQYGRVVKVGKGRRETVQGEVPLSIKEGDKVVIKLDKKVIEIEVNGKKYYICDESEILAIVEE; D: MKLKALGGKIIFELEEPVKPEKGGAIVVGDPGEESQYGRVVKVGKGRRETVQGEVPLSIKEGDKVVIKLDKKVIEIEVNGKKYYICDESEILAIVEE; G: MKLKALGGKIIFELEEPVKPEKGGAIVVGDPGEESQYGRVVKVGKGRRETVQGEVPLSIKEGDKVVIKLDKKVIEIEVNGKKYYICDESEILAIVEE; F: MKLKALGGKIIFELEEPVKPEKGGAIVVGDPGEESQYGRVVKVGKGRRETVQGEVPLSIKEGDKVVIKLDKKVIEIEVNGKKYYICDESEILAIVEE
Selected Chain(s)	A,C,B,E,D,G,F
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:19:15)
[INFO]       Main:     Simulation completed successfully.                                          (00:19:21)

• Minimal score value: -3.591
• Maximal score value: 3.064
• Average score: -1.0691
• Total score value: -725.9258

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	M	A	-0.2407
2	K	A	-1.9012
3	L	A	0.0000
4	K	A	-1.3643
5	A	A	0.0000
6	L	A	0.0000
7	G	A	-1.4457
8	G	A	0.0000
9	K	A	-1.0734
10	I	A	0.0000
11	I	A	0.0000
12	F	A	0.0000
13	E	A	-1.1033
14	L	A	-0.7154
15	E	A	-1.0112
16	E	A	-1.8026
17	P	A	-0.8249
18	V	A	-0.0342
19	K	A	-2.0849
20	P	A	-1.9231
21	E	A	-3.2167
22	K	A	-3.0128
23	G	A	-1.8036
24	G	A	-1.3652
25	A	A	1.0081
26	I	A	3.0193
27	V	A	2.7166
28	V	A	2.5334
29	G	A	-0.2841
30	D	A	-2.0996
31	P	A	-1.6665
32	G	A	-2.4732
33	E	A	-2.9877
34	E	A	-2.8852
35	S	A	-1.7974
36	Q	A	-0.8866
37	Y	A	-0.1288
38	G	A	0.0000
39	R	A	-1.0156
40	V	A	0.0000
41	V	A	-0.9744
42	K	A	-1.6739
43	V	A	-1.6697
44	G	A	0.0000
45	K	A	-2.1256
46	G	A	0.0000
47	R	A	-1.8253
48	R	A	-1.7075
49	E	A	-2.2817
50	T	A	-1.5764
51	V	A	-0.9284
52	Q	A	-1.3781
53	G	A	-0.9610
54	E	A	-1.4363
55	V	A	0.0000
56	P	A	-0.9773
57	L	A	0.0000
58	S	A	-0.8350
59	I	A	0.0000
60	K	A	-2.8139
61	E	A	-2.9697
62	G	A	-2.0041
63	D	A	-2.2889
64	K	A	-1.6268
65	V	A	0.0000
66	V	A	0.0000
67	I	A	0.0000
68	K	A	-1.6514
69	L	A	-1.5454
70	D	A	-2.2103
71	K	A	-2.7950
72	K	A	-2.6102
73	V	A	-1.4084
74	I	A	-1.2693
75	E	A	-1.7338
76	I	A	0.0000
77	E	A	-3.2688
78	V	A	0.0000
79	N	A	-2.4109
80	G	A	-2.3935
81	K	A	-2.7446
82	K	A	-2.8415
83	Y	A	0.0000
84	Y	A	0.0000
85	I	A	0.0000
86	C	A	0.0000
87	D	A	-1.2283
88	E	A	-0.8307
89	S	A	-0.6718
90	E	A	-0.9371
91	I	A	0.0000
92	L	A	0.0000
93	A	A	0.0000
94	I	A	0.0000
95	V	A	0.0000
96	E	A	-2.8256
97	E	A	-2.8201
1	M	B	-0.3548
2	K	B	-1.8545
3	L	B	0.0000
4	K	B	-1.3965
5	A	B	0.0000
6	L	B	0.0000
7	G	B	-1.5204
8	G	B	0.0000
9	K	B	-1.0652
10	I	B	0.0000
11	I	B	0.0000
12	F	B	0.0000
13	E	B	-1.5154
14	L	B	-0.8261
15	E	B	-1.0818
16	E	B	-1.8330
17	P	B	-0.8249
18	V	B	-0.0357
19	K	B	-2.0806
20	P	B	-1.9081
21	E	B	-3.2174
22	K	B	-3.0090
23	G	B	-1.8015
24	G	B	-1.3800
25	A	B	1.0084
26	I	B	3.0194
27	V	B	2.6467
28	V	B	2.3229
29	G	B	-0.3422
30	D	B	-2.1473
31	P	B	-1.7037
32	G	B	-2.5522
33	E	B	-3.1582
34	E	B	-2.7708
35	S	B	-1.8787
36	Q	B	-0.9029
37	Y	B	-0.1473
38	G	B	0.0000
39	R	B	-1.0880
40	V	B	0.0000
41	V	B	-1.0536
42	K	B	-1.7688
43	V	B	-1.6796
44	G	B	0.0000
45	K	B	-2.2172
46	G	B	0.0000
47	R	B	-2.0125
48	R	B	-2.0050
49	E	B	-2.4488
50	T	B	-1.6423
51	V	B	-0.9839
52	Q	B	-1.3982
53	G	B	-1.0551
54	E	B	-1.6583
55	V	B	0.0000
56	P	B	-1.1051
57	L	B	0.0000
58	S	B	-0.7959
59	I	B	0.0000
60	K	B	-2.7147
61	E	B	-2.9861
62	G	B	-1.9970
63	D	B	-2.2572
64	K	B	-1.6149
65	V	B	0.0000
66	V	B	0.0000
67	I	B	0.0000
68	K	B	-1.7640
69	L	B	-1.6584
70	D	B	-2.2983
71	K	B	-2.9363
72	K	B	-2.7306
73	V	B	-1.5451
74	I	B	-1.5465
75	E	B	-2.3168
76	I	B	0.0000
77	E	B	-3.5910
78	V	B	0.0000
79	N	B	-2.6091
80	G	B	-2.6614
81	K	B	-3.5153
82	K	B	-3.5139
83	Y	B	0.0000
84	Y	B	-1.0053
85	I	B	0.0000
86	C	B	0.0000
87	D	B	-1.2078
88	E	B	-0.8153
89	S	B	-0.6799
90	E	B	-0.9774
91	I	B	0.0000
92	L	B	0.0000
93	A	B	0.0000
94	I	B	0.0000
95	V	B	-1.6145
96	E	B	-2.9062
97	E	B	-2.8503
1	M	C	-0.2180
2	K	C	-1.8249
3	L	C	0.0000
4	K	C	-1.3484
5	A	C	0.0000
6	L	C	0.0000
7	G	C	-1.4756
8	G	C	0.0000
9	K	C	-0.9818
10	I	C	0.0000
11	I	C	0.0000
12	F	C	0.0000
13	E	C	-1.1615
14	L	C	-0.7958
15	E	C	-1.0803
16	E	C	-1.8405
17	P	C	-0.8535
18	V	C	-0.0569
19	K	C	-2.0753
20	P	C	-1.8617
21	E	C	-3.1745
22	K	C	-2.9623
23	G	C	-1.7313
24	G	C	-1.2648
25	A	C	1.0817
26	I	C	3.0582
27	V	C	2.6498
28	V	C	2.3564
29	G	C	-0.2765
30	D	C	-2.0840
31	P	C	-1.6252
32	G	C	-2.4832
33	E	C	-3.0045
34	E	C	-2.5261
35	S	C	-1.8030
36	Q	C	-0.8603
37	Y	C	-0.1699
38	G	C	0.0000
39	R	C	-0.8274
40	V	C	0.0000
41	V	C	-0.6081
42	K	C	-1.2850
43	V	C	-1.3232
44	G	C	0.0000
45	K	C	-2.1803
46	G	C	0.0000
47	R	C	-1.8700
48	R	C	-1.9093
49	E	C	-2.0451
50	T	C	-1.5035
51	V	C	-1.0731
52	Q	C	-1.4433
53	G	C	-1.0493
54	E	C	-1.6176
55	V	C	0.0000
56	P	C	-1.0319
57	L	C	-0.9817
58	S	C	-0.8171
59	I	C	0.0000
60	K	C	-2.2716
61	E	C	-1.8975
62	G	C	-1.4753
63	D	C	-1.9519
64	K	C	-1.4926
65	V	C	0.0000
66	V	C	0.0000
67	I	C	0.0000
68	K	C	-1.5743
69	L	C	-1.6256
70	D	C	-2.3776
71	K	C	-2.9649
72	K	C	-2.7364
73	V	C	-1.5229
74	I	C	-1.5197
75	E	C	-2.0765
76	I	C	0.0000
77	E	C	-3.4199
78	V	C	0.0000
79	N	C	-2.4552
80	G	C	-2.4671
81	K	C	-2.9082
82	K	C	-3.1762
83	Y	C	0.0000
84	Y	C	0.0000
85	I	C	0.0000
86	C	C	0.0000
87	D	C	-1.1065
88	E	C	-0.7603
89	S	C	-0.6733
90	E	C	-1.0088
91	I	C	0.0000
92	L	C	0.0000
93	A	C	0.0000
94	I	C	0.0000
95	V	C	0.0000
96	E	C	-2.8659
97	E	C	-2.8234
1	M	D	-0.2922
2	K	D	-1.9307
3	L	D	0.0000
4	K	D	-1.4289
5	A	D	0.0000
6	L	D	0.0000
7	G	D	-1.4411
8	G	D	0.0000
9	K	D	-1.0674
10	I	D	0.0000
11	I	D	0.0000
12	F	D	0.0000
13	E	D	-1.1697
14	L	D	-0.7933
15	E	D	-1.0929
16	E	D	-1.8456
17	P	D	-0.8566
18	V	D	-0.0504
19	K	D	-2.0811
20	P	D	-1.9141
21	E	D	-3.1777
22	K	D	-2.9736
23	G	D	-1.7329
24	G	D	-1.2715
25	A	D	1.0866
26	I	D	3.0640
27	V	D	2.6822
28	V	D	2.3162
29	G	D	-0.4394
30	D	D	-2.1437
31	P	D	-1.7045
32	G	D	-2.5619
33	E	D	-3.0992
34	E	D	-2.7531
35	S	D	-1.8554
36	Q	D	-0.9418
37	Y	D	-0.1897
38	G	D	0.0000
39	R	D	-0.8768
40	V	D	0.0000
41	V	D	-0.6724
42	K	D	-1.4561
43	V	D	-1.4301
44	G	D	0.0000
45	K	D	-2.1265
46	G	D	0.0000
47	R	D	-1.7795
48	R	D	-1.6136
49	E	D	-2.0966
50	T	D	-1.4389
51	V	D	-0.8963
52	Q	D	-1.2942
53	G	D	-0.8787
54	E	D	-1.3345
55	V	D	0.0000
56	P	D	-1.0100
57	L	D	-0.9517
58	S	D	-0.8113
59	I	D	0.0000
60	K	D	-2.2977
61	E	D	-1.9606
62	G	D	-1.4972
63	D	D	-1.9740
64	K	D	-1.5251
65	V	D	0.0000
66	V	D	0.0000
67	I	D	0.0000
68	K	D	-1.6472
69	L	D	-1.6740
70	D	D	-2.3495
71	K	D	-2.9854
72	K	D	-2.7586
73	V	D	-1.5811
74	I	D	-1.6483
75	E	D	-2.2930
76	I	D	0.0000
77	E	D	-3.3343
78	V	D	0.0000
79	N	D	-2.3822
80	G	D	-2.3370
81	K	D	-2.6398
82	K	D	-2.9448
83	Y	D	0.0000
84	Y	D	0.0000
85	I	D	0.0000
86	C	D	0.0000
87	D	D	-1.2366
88	E	D	-0.8029
89	S	D	-0.6642
90	E	D	-0.9748
91	I	D	0.0000
92	L	D	0.0000
93	A	D	0.0000
94	I	D	0.0000
95	V	D	0.0000
96	E	D	-3.0343
97	E	D	-2.9013
1	M	E	-0.2028
2	K	E	-1.8086
3	L	E	0.0000
4	K	E	-1.4684
5	A	E	0.0000
6	L	E	0.0000
7	G	E	-1.4575
8	G	E	0.0000
9	K	E	-1.1250
10	I	E	0.0000
11	I	E	0.0000
12	F	E	0.0000
13	E	E	-1.2946
14	L	E	-0.9340
15	E	E	-1.1732
16	E	E	-1.8786
17	P	E	-0.8723
18	V	E	-0.0514
19	K	E	-2.0701
20	P	E	-1.8770
21	E	E	-3.1898
22	K	E	-2.9849
23	G	E	-1.7566
24	G	E	-1.2659
25	A	E	1.0616
26	I	E	3.0432
27	V	E	2.7460
28	V	E	2.3416
29	G	E	-0.2860
30	D	E	-2.0481
31	P	E	-1.5990
32	G	E	-2.4244
33	E	E	-2.7867
34	E	E	-2.5791
35	S	E	-1.7480
36	Q	E	-0.9332
37	Y	E	-0.2498
38	G	E	0.0000
39	R	E	-1.1113
40	V	E	0.0000
41	V	E	-0.9718
42	K	E	-1.7085
43	V	E	-1.6838
44	G	E	0.0000
45	K	E	-2.1532
46	G	E	0.0000
47	R	E	-1.7619
48	R	E	-1.5460
49	E	E	-1.8936
50	T	E	-1.3438
51	V	E	-0.8498
52	Q	E	-1.2990
53	G	E	-0.8553
54	E	E	-1.2993
55	V	E	0.0000
56	P	E	-1.0364
57	L	E	0.0000
58	S	E	-0.8150
59	I	E	0.0000
60	K	E	-2.7253
61	E	E	-2.9747
62	G	E	-1.9880
63	D	E	-2.2609
64	K	E	-1.6469
65	V	E	0.0000
66	V	E	0.0000
67	I	E	0.0000
68	K	E	-1.6566
69	L	E	-1.7597
70	D	E	-2.4116
71	K	E	-3.0517
72	K	E	-2.8468
73	V	E	-1.7092
74	I	E	-1.7613
75	E	E	-2.5409
76	I	E	0.0000
77	E	E	-3.3909
78	V	E	0.0000
79	N	E	-2.3645
80	G	E	-2.3237
81	K	E	-2.6492
82	K	E	-3.0479
83	Y	E	0.0000
84	Y	E	0.0000
85	I	E	0.0000
86	C	E	0.0000
87	D	E	-1.2586
88	E	E	-0.8284
89	S	E	-0.6891
90	E	E	-1.0574
91	I	E	0.0000
92	L	E	-0.2924
93	A	E	0.0000
94	I	E	0.0000
95	V	E	0.0000
96	E	E	-2.7802
97	E	E	-2.7875
1	M	F	-0.2763
2	K	F	-1.8885
3	L	F	0.0000
4	K	F	-1.4984
5	A	F	0.0000
6	L	F	0.0000
7	G	F	-1.5516
8	G	F	0.0000
9	K	F	-1.1011
10	I	F	0.0000
11	I	F	0.0000
12	F	F	0.0000
13	E	F	-1.1320
14	L	F	-0.8606
15	E	F	-1.1494
16	E	F	-1.8736
17	P	F	-0.8702
18	V	F	-0.0509
19	K	F	-2.0817
20	P	F	-1.9141
21	E	F	-3.2200
22	K	F	-3.0059
23	G	F	-1.8044
24	G	F	-1.4089
25	A	F	0.9984
26	I	F	3.0155
27	V	F	2.7625
28	V	F	2.4025
29	G	F	-0.1749
30	D	F	-1.9657
31	P	F	-1.5404
32	G	F	-2.3416
33	E	F	-2.6350
34	E	F	-2.2673
35	S	F	-1.5702
36	Q	F	-0.8078
37	Y	F	-0.1676
38	G	F	0.0000
39	R	F	-0.8721
40	V	F	0.0000
41	V	F	-0.6279
42	K	F	-1.3665
43	V	F	-1.3938
44	G	F	0.0000
45	K	F	-2.1534
46	G	F	0.0000
47	R	F	-2.0017
48	R	F	-1.9164
49	E	F	-2.5893
50	T	F	-1.6181
51	V	F	-0.9236
52	Q	F	-1.3787
53	G	F	-0.9851
54	E	F	-1.4707
55	V	F	0.0000
56	P	F	-0.9887
57	L	F	-0.9397
58	S	F	-0.7612
59	I	F	0.0000
60	K	F	-2.2647
61	E	F	-1.9061
62	G	F	-1.4693
63	D	F	-1.9599
64	K	F	-1.4999
65	V	F	0.0000
66	V	F	0.0000
67	I	F	0.0000
68	K	F	-1.4464
69	L	F	-1.6461
70	D	F	-2.6126
71	K	F	-3.1228
72	K	F	-2.8679
73	V	F	-1.6465
74	I	F	-1.5933
75	E	F	-2.0176
76	I	F	0.0000
77	E	F	-3.2306
78	V	F	0.0000
79	N	F	-2.3668
80	G	F	-2.3428
81	K	F	-2.6310
82	K	F	-2.7940
83	Y	F	0.0000
84	Y	F	0.0000
85	I	F	0.0000
86	C	F	0.0000
87	D	F	-1.2940
88	E	F	-0.8356
89	S	F	-0.7005
90	E	F	-1.0805
91	I	F	0.0000
92	L	F	0.0000
93	A	F	0.0000
94	I	F	0.0000
95	V	F	0.0000
96	E	F	-2.9076
97	E	F	-2.8644
1	M	G	-0.2740
2	K	G	-1.8637
3	L	G	0.0000
4	K	G	-1.4613
5	A	G	0.0000
6	L	G	0.0000
7	G	G	-1.4990
8	G	G	0.0000
9	K	G	-1.0696
10	I	G	0.0000
11	I	G	0.0000
12	F	G	0.0000
13	E	G	-1.1143
14	L	G	-0.8341
15	E	G	-1.1368
16	E	G	-1.8760
17	P	G	-0.8818
18	V	G	-0.0659
19	K	G	-2.0928
20	P	G	-1.8549
21	E	G	-3.1812
22	K	G	-2.9689
23	G	G	-1.7341
24	G	G	-1.0683
25	A	G	1.0857
26	I	G	3.0618
27	V	G	2.7165
28	V	G	2.3768
29	G	G	-0.2339
30	D	G	-2.0539
31	P	G	-1.5484
32	G	G	-2.4240
33	E	G	-2.9260
34	E	G	-2.3255
35	S	G	-1.6844
36	Q	G	-0.8722
37	Y	G	-0.1984
38	G	G	0.0000
39	R	G	-1.0614
40	V	G	0.0000
41	V	G	-1.1207
42	K	G	-1.9552
43	V	G	-1.7621
44	G	G	0.0000
45	K	G	-2.1795
46	G	G	0.0000
47	R	G	-1.9905
48	R	G	-2.0333
49	E	G	-2.3461
50	T	G	-1.5421
51	V	G	-0.9869
52	Q	G	-1.4212
53	G	G	-1.0212
54	E	G	-1.5540
55	V	G	0.0000
56	P	G	-0.9997
57	L	G	-0.9271
58	S	G	-0.7493
59	I	G	0.0000
60	K	G	-2.8569
61	E	G	-3.1159
62	G	G	-2.0777
63	D	G	-2.3485
64	K	G	-1.6611
65	V	G	0.0000
66	V	G	0.0000
67	I	G	0.0000
68	K	G	-1.5897
69	L	G	-1.5670
70	D	G	-2.3439
71	K	G	-2.9070
72	K	G	-2.7520
73	V	G	-1.5243
74	I	G	-1.3830
75	E	G	-1.7765
76	I	G	0.0000
77	E	G	-3.1442
78	V	G	0.0000
79	N	G	-2.3386
80	G	G	-2.2647
81	K	G	-2.5136
82	K	G	-2.6306
83	Y	G	0.0000
84	Y	G	0.0000
85	I	G	0.0000
86	C	G	0.0000
87	D	G	-1.2499
88	E	G	-0.8115
89	S	G	-0.7099
90	E	G	-1.0607
91	I	G	0.0000
92	L	G	-0.2827
93	A	G	0.0000
94	I	G	0.0000
95	V	G	0.0000
96	E	G	-2.9031
97	E	G	-2.8489