Project name: obj1 [mutate: QR3C, GD8C, GC10C, SG25C, TV28C, EW33C, LM45C, WH47C, TI58C, TD69C, KR76C, QT82C, LS86C, IP93C, DH101C, TE114C]

Chain sequence(s)	C: EVQLVESGGGLVQPGGSLRLSCAASDFTFRSYEMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARLRDGFNKGFDYWGQGTLVTVSS
Selected Chain(s)	C
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No
Mutated residues	KR76C,LM45C,QT82C,WH47C,LS86C,SG25C,TV28C,TD69C,IP93C,TI58C,GD8C,QR3C,TE114C,GC10C,DH101C,EW33C
Energy difference between WT (input) and mutated protein (by FoldX)	18.5079 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with C chain(s) selected             (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       FoldX:    Building mutant model                                                       (00:00:24)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:03:49)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:04:17)
[INFO]       Main:     Simulation completed successfully.                                          (00:04:18)

• Minimal score value: -3.0127
• Maximal score value: 1.8848
• Average score: -0.667
• Total score value: -80.0385

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	C	-2.1678
2	V	C	-1.2537
3	R	C	-2.1317	mutated: QR3C
4	L	C	0.0000
5	V	C	-0.2025
6	E	C	0.0000
7	S	C	-0.8151
8	D	C	-1.4147	mutated: GD8C
9	G	C	0.0110
10	C	C	1.5499	mutated: GC10C
11	L	C	1.8848
12	V	C	0.1348
13	Q	C	-1.3563
14	P	C	-1.5021
15	G	C	-1.4286
16	G	C	-1.0478
17	S	C	-1.2419
18	L	C	0.0000
19	R	C	-2.1294
20	L	C	0.0000
21	S	C	-0.7755
22	C	C	0.0000
23	A	C	-0.5622
24	A	C	0.0000
25	G	C	-0.6754	mutated: SG25C
26	D	C	0.0000
27	F	C	1.7214
28	V	C	0.7193	mutated: TV28C
29	F	C	0.0000
30	R	C	-1.7453
31	S	C	-0.5570
32	Y	C	-0.5884
33	W	C	-0.1828	mutated: EW33C
34	M	C	0.0000
35	S	C	0.0000
36	W	C	0.0000
37	V	C	0.0000
38	R	C	0.0000
39	Q	C	-0.9197
40	A	C	-1.2744
41	P	C	-1.4043
42	G	C	-1.5027
43	K	C	-2.2803
44	G	C	-1.3577
45	M	C	-0.5004	mutated: LM45C
46	E	C	-1.1435
47	H	C	-0.7343	mutated: WH47C
48	V	C	0.0000
49	S	C	0.0000
50	A	C	0.0000
51	I	C	0.0000
52	S	C	0.0337
53	G	C	-0.9036
54	S	C	-1.1157
55	G	C	-0.7037
56	G	C	-0.4496
57	S	C	0.4607
58	I	C	1.5579	mutated: TI58C
59	Y	C	1.4300
60	Y	C	-0.1972
61	A	C	-1.2652
62	D	C	-2.3036
63	S	C	-1.7130
64	V	C	0.0000
65	K	C	-2.4855
66	G	C	-1.7930
67	R	C	0.0000
68	F	C	0.0000
69	D	C	-1.3489	mutated: TD69C
70	I	C	0.0000
71	S	C	-0.6409
72	R	C	-1.4346
73	D	C	-2.0499
74	N	C	-2.1809
75	S	C	-1.8579
76	R	C	-2.4592	mutated: KR76C
77	N	C	-1.7104
78	T	C	0.0000
79	L	C	0.0000
80	Y	C	0.0000
81	L	C	0.0000
82	T	C	-1.2321	mutated: QT82C
83	M	C	0.0000
84	N	C	-1.4208
85	S	C	-1.2667
86	S	C	0.0000	mutated: LS86C
87	R	C	-2.4804
88	A	C	-1.8930
89	E	C	-2.3450
90	D	C	0.0000
91	T	C	-0.4642
92	A	C	0.0000
93	P	C	0.2922	mutated: IP93C
94	Y	C	0.0000
95	Y	C	0.0953
96	C	C	0.0000
97	A	C	0.0000
98	R	C	0.0000
99	L	C	0.0000
100	R	C	-2.6927
101	H	C	-2.4365	mutated: DH101C
102	G	C	-1.6177
103	F	C	-1.0649
104	N	C	-2.3831
105	K	C	-3.0127
106	G	C	-1.8148
107	F	C	-0.8560
108	D	C	-0.9929
109	Y	C	-0.3979
110	W	C	0.2184
111	G	C	-0.5112
112	Q	C	-1.2874
113	G	C	-0.3925
114	E	C	-0.1652	mutated: TE114C
115	L	C	1.4196
116	V	C	0.0000
117	T	C	0.5118
118	V	C	0.0000
119	S	C	-0.7805
120	S	C	-1.0628