Project name: NT11???

Status: done

Started: 2026-02-28 14:10:18
Settings
Chain sequence(s) A: MVSEPHDYNYEKANTPHGGVLKDLFARDAAKHDELLNEAQSLPKLKLSDRQLCDLELILNGGFSPLEGFLSEKDYIGVRDNMALADGSLWAIPINLDVSKDEIDKLSIAPGRRIVLQDSRDDNLLAIITVEDVYQPDKALEAKNVFGGDPEHPAIVYLNESVKDYYVGGKVQAISRPFHYDYVGLRHTPAELRAEFAKLGWTRVVAFQTRNPMHRAHRELTVRAARSQRANVLIQPVVGLTKPGDIDHFTRVRVYKALLPRYPNGMATLSLLPLAMRMGGPREAVWHACIRKNYGASHFIIGRDHAGPGKNSKGVDFYGPYDAQTLVESVKDKLDIEIVPFQMMTYLPDKDIYMPKDEVPEGVKTLDISGTELRRRLRLGLPIPEWFSYPEVVKVLRESNPPSTNITFHASALTRSERTELRNQRGLTIWLTGLSASGKSTLAVELEHQLVRDRRVHAYRLDGDNIRFGLNKDLGFSEADRNENIRRIAEVAKLFADSNSIAITSFISPYRKDRDTARQLHEVATPGEETGLPFVEVYVDVPVEVAEQRDPKGLYKKAREGVIKEFTGISAPYEAPANPEVHVKNYELPVQDAVKQIIDYLDTKGYLPAKKEKLAAALEHHHHHH
input PDB
Selected Chain(s) A
Distance of aggregation 5 Å
FoldX usage Yes
Dynamic mode No
Automated mutations Yes
Downloads Download all the data
Simulation log 
[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:02)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:02)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with A chain(s) selected             (00:00:02)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:02)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:02)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:13:55)
[INFO]       Auto_mut: Residue number 2 from chain A and a score of 1.928 (valine) selected for    
                       automated muatation                                                         (00:14:03)
[INFO]       Auto_mut: Residue number 178 from chain A and a score of 1.902 (phenylalanine)        
                       selected for automated muatation                                            (00:14:03)
[INFO]       Auto_mut: Residue number 408 from chain A and a score of 1.859 (phenylalanine)        
                       selected for automated muatation                                            (00:14:03)
[INFO]       Auto_mut: Residue number 476 from chain A and a score of 1.855 (phenylalanine)        
                       selected for automated muatation                                            (00:14:03)
[INFO]       Auto_mut: Residue number 562 from chain A and a score of 1.830 (valine) selected for  
                       automated muatation                                                         (00:14:03)
[INFO]       Auto_mut: Residue number 183 from chain A and a score of 1.719 (valine) selected for  
                       automated muatation                                                         (00:14:03)
[INFO]       Auto_mut: Mutating residue number 2 from chain A (valine) into glutamic acid          (00:14:03)
[INFO]       Auto_mut: Mutating residue number 2 from chain A (valine) into aspartic acid          (00:14:03)
[INFO]       Auto_mut: Mutating residue number 178 from chain A (phenylalanine) into glutamic acid 
                       Mutating residue number 178 from chain A (phenylalanine) into glutamic acid (00:14:03)
[INFO]       Auto_mut: Mutating residue number 2 from chain A (valine) into lysine                 (00:21:19)
[INFO]       Auto_mut: Mutating residue number 2 from chain A (valine) into arginine               (00:21:21)
[INFO]       Auto_mut: Mutating residue number 178 from chain A (phenylalanine) into lysine        (00:21:39)
[INFO]       Auto_mut: Mutating residue number 178 from chain A (phenylalanine) into aspartic acid 
                       Mutating residue number 178 from chain A (phenylalanine) into aspartic acid (00:28:36)
[INFO]       Auto_mut: Mutating residue number 408 from chain A (phenylalanine) into glutamic acid 
                       Mutating residue number 408 from chain A (phenylalanine) into glutamic acid (00:28:44)
[INFO]       Auto_mut: Mutating residue number 408 from chain A (phenylalanine) into aspartic acid 
                       Mutating residue number 408 from chain A (phenylalanine) into aspartic acid (00:29:33)
[INFO]       Auto_mut: Mutating residue number 178 from chain A (phenylalanine) into arginine      (00:36:07)
[INFO]       Auto_mut: Mutating residue number 408 from chain A (phenylalanine) into lysine        (00:36:14)
[INFO]       Auto_mut: Mutating residue number 408 from chain A (phenylalanine) into arginine      (00:36:54)
[INFO]       Auto_mut: Mutating residue number 476 from chain A (phenylalanine) into glutamic acid 
                       Mutating residue number 476 from chain A (phenylalanine) into glutamic acid (00:43:40)
[INFO]       Auto_mut: Mutating residue number 476 from chain A (phenylalanine) into aspartic acid 
                       Mutating residue number 476 from chain A (phenylalanine) into aspartic acid (00:43:48)
[INFO]       Auto_mut: Mutating residue number 562 from chain A (valine) into glutamic acid        (00:44:35)
[INFO]       Auto_mut: Mutating residue number 476 from chain A (phenylalanine) into lysine        (00:51:41)
[INFO]       Auto_mut: Mutating residue number 476 from chain A (phenylalanine) into arginine      (00:51:44)
[INFO]       Auto_mut: Mutating residue number 562 from chain A (valine) into lysine               (00:52:35)
[INFO]       Auto_mut: Mutating residue number 562 from chain A (valine) into aspartic acid        (00:59:50)
[INFO]       Auto_mut: Mutating residue number 183 from chain A (valine) into glutamic acid        (00:59:51)
[INFO]       Auto_mut: Mutating residue number 183 from chain A (valine) into aspartic acid        (01:00:21)
[INFO]       Auto_mut: Mutating residue number 562 from chain A (valine) into arginine             (01:07:37)
[INFO]       Auto_mut: Mutating residue number 183 from chain A (valine) into lysine               (01:07:40)
[INFO]       Auto_mut: Mutating residue number 183 from chain A (valine) into arginine             (01:08:14)
[INFO]       Auto_mut: Effect of mutation residue number 2 from chain A (valine) into glutamic     
                       acid: Energy difference: -0.6494 kcal/mol, Difference in average score from 
                       the base case: -0.0046                                                      (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 2 from chain A (valine) into lysine:      
                       Energy difference: -0.2330 kcal/mol, Difference in average score from the   
                       base case: -0.0041                                                          (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 2 from chain A (valine) into aspartic     
                       acid: Energy difference: -0.4381 kcal/mol, Difference in average score from 
                       the base case: -0.0045                                                      (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 2 from chain A (valine) into arginine:    
                       Energy difference: -0.3741 kcal/mol, Difference in average score from the   
                       base case: -0.0050                                                          (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 178 from chain A (phenylalanine) into     
                       glutamic acid: Energy difference: 0.5696 kcal/mol, Difference in average    
                       score from the base case: -0.0046                                           (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 178 from chain A (phenylalanine) into     
                       lysine: Energy difference: 0.0966 kcal/mol, Difference in average score     
                       from the base case: -0.0037                                                 (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 178 from chain A (phenylalanine) into     
                       aspartic acid: Energy difference: 0.7520 kcal/mol, Difference in average    
                       score from the base case: -0.0053                                           (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 178 from chain A (phenylalanine) into     
                       arginine: Energy difference: -0.7417 kcal/mol, Difference in average score  
                       from the base case: -0.0038                                                 (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 408 from chain A (phenylalanine) into     
                       glutamic acid: Energy difference: 0.8612 kcal/mol, Difference in average    
                       score from the base case: -0.0049                                           (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 408 from chain A (phenylalanine) into     
                       lysine: Energy difference: 0.1186 kcal/mol, Difference in average score     
                       from the base case: -0.0051                                                 (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 408 from chain A (phenylalanine) into     
                       aspartic acid: Energy difference: 1.3359 kcal/mol, Difference in average    
                       score from the base case: -0.0046                                           (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 408 from chain A (phenylalanine) into     
                       arginine: Energy difference: 0.0667 kcal/mol, Difference in average score   
                       from the base case: -0.0056                                                 (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 476 from chain A (phenylalanine) into     
                       glutamic acid: Energy difference: -0.0430 kcal/mol, Difference in average   
                       score from the base case: -0.0056                                           (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 476 from chain A (phenylalanine) into     
                       lysine: Energy difference: 0.1189 kcal/mol, Difference in average score     
                       from the base case: -0.0069                                                 (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 476 from chain A (phenylalanine) into     
                       aspartic acid: Energy difference: -0.3040 kcal/mol, Difference in average   
                       score from the base case: -0.0058                                           (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 476 from chain A (phenylalanine) into     
                       arginine: Energy difference: 0.0553 kcal/mol, Difference in average score   
                       from the base case: -0.0060                                                 (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 562 from chain A (valine) into glutamic   
                       acid: Energy difference: 0.0805 kcal/mol, Difference in average score from  
                       the base case: -0.0044                                                      (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 562 from chain A (valine) into lysine:    
                       Energy difference: -0.4932 kcal/mol, Difference in average score from the   
                       base case: -0.0042                                                          (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 562 from chain A (valine) into aspartic   
                       acid: Energy difference: 0.4365 kcal/mol, Difference in average score from  
                       the base case: -0.0040                                                      (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 562 from chain A (valine) into arginine:  
                       Energy difference: -0.8588 kcal/mol, Difference in average score from the   
                       base case: -0.0048                                                          (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 183 from chain A (valine) into glutamic   
                       acid: Energy difference: -0.2591 kcal/mol, Difference in average score from 
                       the base case: -0.0045                                                      (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 183 from chain A (valine) into lysine:    
                       Energy difference: -0.2257 kcal/mol, Difference in average score from the   
                       base case: -0.0043                                                          (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 183 from chain A (valine) into aspartic   
                       acid: Energy difference: -0.1919 kcal/mol, Difference in average score from 
                       the base case: -0.0044                                                      (01:15:39)
[INFO]       Auto_mut: Effect of mutation residue number 183 from chain A (valine) into arginine:  
                       Energy difference: -0.6485 kcal/mol, Difference in average score from the   
                       base case: -0.0043                                                          (01:15:39)
[INFO]       Main:     Simulation completed successfully.                                          (01:15:51)
Show buried residues
Minimal score value
-2.4528
Maximal score value
1.9281
Average score
-0.3394
Total score value
-212.0958
Download the table (csv)

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index residue name chain Aggrescan3D score mutation
residue index residue name chain Aggrescan3D score
mutation
1 M A 1.3805
2 V A 1.9281
3 S A -0.2228
4 E A -1.9056
5 P A -0.7724
6 H A -1.3676
7 D A -1.7421
8 Y A 0.6622
9 N A -0.9335
10 Y A 0.0421
11 E A -1.8762
12 K A -1.2226
13 A A -0.1950
14 N A -0.2157
15 T A -0.0932
16 P A -0.0383
17 H A 0.0000
18 G A -0.5495
19 G A -0.2964
20 V A 1.3687
21 L A 0.4016
22 K A -0.2865
23 D A -0.3128
24 L A 0.0000
25 F A 0.3391
26 A A -0.0775
27 R A -1.0848
28 D A 0.0000
29 A A 0.0690
30 A A -0.0653
31 K A -0.8369
32 H A -0.9536
33 D A -2.2060
34 E A -2.1419
35 L A 0.0000
36 L A 0.3512
37 N A -1.2701
38 E A -0.7942
39 A A 0.0000
40 Q A -1.2366
41 S A -0.3836
42 L A 0.1990
43 P A -0.1085
44 K A -0.5559
45 L A 0.0000
46 K A -1.7152
47 L A 0.0000
48 S A -0.4151
49 D A -1.8534
50 R A -0.5836
51 Q A 0.0000
52 L A 0.0000
53 C A 0.0000
54 D A 0.0000
55 L A 0.0000
56 E A 0.0000
57 L A 0.0000
58 I A 0.0000
59 L A 0.0000
60 N A 0.0000
61 G A 0.0000
62 G A 0.0000
63 F A 0.0000
64 S A 0.0000
65 P A -0.0348
66 L A 0.0000
67 E A -0.4505
68 G A 0.0000
69 F A 0.0000
70 L A 0.0000
71 S A -0.2206
72 E A -1.3948
73 K A -1.9621
74 D A -0.6622
75 Y A 0.0000
76 I A 0.6309
77 G A 0.0494
78 V A 0.0000
79 R A 0.0000
80 D A -1.0227
81 N A -1.3963
82 M A -0.1123
83 A A 0.0292
84 L A 0.0000
85 A A -0.2978
86 D A -1.8355
87 G A -0.6436
88 S A -0.0998
89 L A 0.0000
90 W A 0.0000
91 A A 0.0000
92 I A 0.0000
93 P A 0.0000
94 I A 0.0000
95 N A -0.1293
96 L A 0.0000
97 D A -0.1985
98 V A 0.0000
99 S A -0.3473
100 K A -2.0324
101 D A -2.1307
102 E A -0.4504
103 I A -0.0909
104 D A -2.0591
105 K A -1.9870
106 L A -0.1617
107 S A -0.3055
108 I A 0.0000
109 A A -0.0179
110 P A -0.3338
111 G A -0.6915
112 R A -1.1602
113 R A -0.5686
114 I A 0.0000
115 V A 0.0000
116 L A 0.0000
117 Q A -0.3071
118 D A 0.0000
119 S A -0.4727
120 R A -1.7090
121 D A -1.7630
122 D A -2.0737
123 N A -0.6959
124 L A 0.0822
125 L A 0.0000
126 A A 0.0000
127 I A 0.0000
128 I A 0.0000
129 T A -0.0715
130 V A 0.0000
131 E A -1.2317
132 D A -0.4132
133 V A 0.3928
134 Y A 0.0000
135 Q A -1.1744
136 P A 0.0000
137 D A -1.7504
138 K A -0.5836
139 A A 0.0823
140 L A 0.4270
141 E A 0.0000
142 A A 0.0000
143 K A -1.8563
144 N A -0.6684
145 V A 0.0000
146 F A 0.0000
147 G A -0.7018
148 G A -0.6730
149 D A -0.5366
150 P A -0.4810
151 E A -0.8571
152 H A 0.0000
153 P A -0.0499
154 A A 0.0000
155 I A 0.0000
156 V A 0.7494
157 Y A 0.4316
158 L A 0.0000
159 N A -1.5983
160 E A -2.0759
161 S A -0.4457
162 V A 0.0924
163 K A -0.8252
164 D A -1.7651
165 Y A -0.2620
166 Y A 0.0000
167 V A 0.0000
168 G A 0.0000
169 G A 0.0000
170 K A -1.3238
171 V A 0.0000
172 Q A -0.2193
173 A A 0.0000
174 I A 0.0000
175 S A -0.1645
176 R A -0.7315
177 P A 0.0000
178 F A 1.9017
179 H A 0.3801
180 Y A 1.2766
181 D A 0.0000
182 Y A 0.4822
183 V A 1.7187
184 G A -0.1098
185 L A 0.1010
186 R A -0.1786
187 H A -0.1807
188 T A 0.0000
189 P A 0.0000
190 A A -0.1738
191 E A -1.0037
192 L A 0.0000
193 R A 0.0000
194 A A -0.0822
195 E A -0.5036
196 F A 0.0000
197 A A -0.3403
198 K A -1.5561
199 L A 0.3960
200 G A -0.3241
201 W A 0.0000
202 T A -0.2882
203 R A -1.2395
204 V A 0.0000
205 V A 0.0000
206 A A 0.0000
207 F A 0.0000
208 Q A -0.1161
209 T A 0.0000
210 R A -0.4508
211 N A -0.6448
212 P A 0.0000
213 M A 0.0000
214 H A 0.0000
215 R A -0.3884
216 A A -0.0983
217 H A -0.2581
218 R A -0.4165
219 E A -0.3030
220 L A 0.0773
221 T A 0.0000
222 V A 0.0114
223 R A -0.8493
224 A A 0.0000
225 A A 0.0000
226 R A -2.0777
227 S A -0.5877
228 Q A -0.6042
229 R A -2.0807
230 A A -0.5202
231 N A -0.2246
232 V A 0.0000
233 L A 0.0000
234 I A 0.0000
235 Q A 0.0000
236 P A 0.0000
237 V A 0.0000
238 V A 0.0000
239 G A -0.0822
240 L A 0.2192
241 T A -0.0736
242 K A -0.6204
243 P A -0.4416
244 G A -0.5924
245 D A -0.4673
246 I A 0.2118
247 D A -0.1905
248 H A -0.2135
249 F A 0.3465
250 T A 0.0000
251 R A 0.0000
252 V A 0.0000
253 R A 0.0000
254 V A 0.0000
255 Y A 0.0000
256 K A -0.8016
257 A A -0.1384
258 L A 0.0000
259 L A 0.2432
260 P A -0.3343
261 R A -0.7784
262 Y A 0.0000
263 P A -0.4102
264 N A -1.3904
265 G A -0.6776
266 M A 0.0331
267 A A 0.0290
268 T A -0.0072
269 L A 0.0000
270 S A 0.0000
271 L A 0.0000
272 L A 0.0000
273 P A 0.0000
274 L A 0.0000
275 A A 0.0000
276 M A 0.0000
277 R A 0.0000
278 M A 0.0000
279 G A 0.0000
280 G A 0.0000
281 P A -0.0610
282 R A -0.1912
283 E A 0.0000
284 A A 0.0000
285 V A 0.0000
286 W A 0.0000
287 H A 0.0000
288 A A 0.0000
289 C A 0.0000
290 I A 0.0000
291 R A 0.0000
292 K A 0.0000
293 N A 0.0000
294 Y A 0.0000
295 G A 0.0000
296 A A 0.0000
297 S A -0.0306
298 H A 0.0000
299 F A 0.0000
300 I A 0.0000
301 I A 0.0000
302 G A -0.2763
303 R A -1.3763
304 D A -0.9796
305 H A 0.0000
306 A A 0.0061
307 G A 0.0000
308 P A 0.0000
309 G A -0.4626
310 K A -2.0251
311 N A -0.5109
312 S A -0.5441
313 K A -1.7923
314 G A -0.2907
315 V A 1.3924
316 D A -1.6676
317 F A 0.0028
318 Y A 0.1888
319 G A -0.1316
320 P A -0.0660
321 Y A 0.6573
322 D A -0.8207
323 A A 0.0000
324 Q A -0.1514
325 T A -0.0398
326 L A 0.2965
327 V A 0.0000
328 E A -1.8580
329 S A -0.5053
330 V A -0.1396
331 K A -1.9199
332 D A -2.3458
333 K A -1.7946
334 L A 0.0000
335 D A -1.7894
336 I A 0.0000
337 E A -1.7735
338 I A -0.0773
339 V A 0.0000
340 P A -0.2119
341 F A 0.1049
342 Q A -0.0691
343 M A 0.9016
344 M A 0.0000
345 T A 0.0000
346 Y A 0.0000
347 L A 0.0000
348 P A -0.5602
349 D A -2.1035
350 K A -2.3402
351 D A -1.9681
352 I A 0.4052
353 Y A 0.0000
354 M A 0.1485
355 P A -0.3084
356 K A -2.0298
357 D A -2.4254
358 E A -2.1018
359 V A -0.1308
360 P A -0.4672
361 E A -1.9357
362 G A -0.7097
363 V A 0.0721
364 K A -1.6191
365 T A -0.2692
366 L A 0.0262
367 D A -1.6829
368 I A -0.1320
369 S A -0.2209
370 G A -0.5111
371 T A -0.2206
372 E A -0.4902
373 L A 0.0000
374 R A -1.4203
375 R A -1.4235
376 R A -0.4932
377 L A 0.0000
378 R A -1.6627
379 L A 0.6321
380 G A 0.0186
381 L A 0.3955
382 P A -0.1224
383 I A 0.1472
384 P A -0.3423
385 E A -1.7980
386 W A -0.1284
387 F A 0.2263
388 S A 0.0000
389 Y A 0.0000
390 P A -0.3458
391 E A -0.5954
392 V A 0.0000
393 V A 0.0000
394 K A -0.5066
395 V A 0.0000
396 L A 0.0000
397 R A -0.3481
398 E A 0.0000
399 S A 0.0000
400 N A 0.0000
401 P A 0.0000
402 P A -0.0482
403 S A -0.0888
404 T A -0.3149
405 N A -1.1458
406 I A 0.5196
407 T A 0.4292
408 F A 1.8586
409 H A -0.0561
410 A A -0.0251
411 S A -0.0324
412 A A 0.1677
413 L A 0.7964
414 T A 0.0000
415 R A 0.0000
416 S A 0.0000
417 E A 0.0000
418 R A -0.3945
419 T A 0.0000
420 E A 0.0000
421 L A -0.0835
422 R A -1.9552
423 N A -1.1996
424 Q A 0.0000
425 R A -0.2614
426 G A 0.0000
427 L A 0.0000
428 T A 0.0000
429 I A 0.0000
430 W A 0.0000
431 L A 0.0000
432 T A 0.0000
433 G A 0.0000
434 L A 0.0000
435 S A -0.0266
436 A A 0.0076
437 S A 0.0000
438 G A -0.1130
439 K A 0.0000
440 S A -0.2252
441 T A -0.1070
442 L A 0.0000
443 A A 0.0000
444 V A 0.6497
445 E A -0.3750
446 L A 0.0000
447 E A -0.3859
448 H A -0.8783
449 Q A 0.0000
450 L A 0.0000
451 V A 0.1803
452 R A -2.0430
453 D A -2.1613
454 R A -0.8374
455 R A -1.7961
456 V A 0.0000
457 H A -0.9901
458 A A 0.0000
459 Y A 0.6290
460 R A -0.2572
461 L A 0.0000
462 D A -0.2844
463 G A -0.7561
464 D A -1.9754
465 N A -0.9579
466 I A 0.0000
467 R A -0.6838
468 F A 1.6706
469 G A 0.0206
470 L A 0.6730
471 N A 0.0000
472 K A -2.0234
473 D A -2.0966
474 L A 0.0000
475 G A -0.0221
476 F A 1.8554
477 S A -0.0721
478 E A -1.8261
479 A A -0.3466
480 D A -0.5674
481 R A -1.0336
482 N A -1.3124
483 E A -0.7536
484 N A -0.4170
485 I A 0.0000
486 R A -0.9333
487 R A -0.4769
488 I A 0.0000
489 A A 0.0000
490 E A -0.8054
491 V A 0.1515
492 A A 0.0000
493 K A 0.0110
494 L A 1.5927
495 F A 0.8353
496 A A 0.0000
497 D A -0.0781
498 S A -0.1702
499 N A 0.0000
500 S A 0.0000
501 I A 0.0000
502 A A 0.0000
503 I A 0.0000
504 T A 0.0000
505 S A 0.0145
506 F A 0.3185
507 I A 0.6489
508 S A 0.0000
509 P A 0.0806
510 Y A 0.2994
511 R A -1.3647
512 K A -1.9437
513 D A -0.5033
514 R A 0.0000
515 D A -1.8188
516 T A -0.3585
517 A A 0.0000
518 R A -0.6549
519 Q A -1.3812
520 L A 0.0673
521 H A 0.0000
522 E A -1.6321
523 V A 1.4392
524 A A 0.3360
525 T A -0.0510
526 P A -0.2680
527 G A -0.1417
528 E A 0.0000
529 E A -0.2028
530 T A -0.0558
531 G A -0.1607
532 L A 0.0000
533 P A -0.0254
534 F A 0.0000
535 V A 0.0000
536 E A 0.0000
537 V A 0.0000
538 Y A 0.1011
539 V A 0.0000
540 D A -0.2614
541 V A 0.0000
542 P A -0.0189
543 V A 0.0727
544 E A -1.6803
545 V A 0.0263
546 A A 0.0000
547 E A -0.4963
548 Q A -1.5810
549 R A -2.0600
550 D A 0.0000
551 P A -0.5592
552 K A -1.7610
553 G A -0.3220
554 L A 0.3536
555 Y A 0.0000
556 K A -1.7817
557 K A -1.3628
558 A A 0.0000
559 R A -2.1735
560 E A -2.2787
561 G A -0.2043
562 V A 1.8302
563 I A 0.5252
564 K A -1.9357
565 E A -2.1152
566 F A -0.1445
567 T A 0.0058
568 G A -0.0317
569 I A 0.4514
570 S A -0.3136
571 A A -0.2661
572 P A -0.1953
573 Y A 0.0260
574 E A -0.6608
575 A A -0.1149
576 P A -0.0118
577 A A -0.1755
578 N A -1.2715
579 P A -0.3707
580 E A -0.5117
581 V A 0.0000
582 H A -0.5300
583 V A 0.0000
584 K A -0.7126
585 N A -0.2298
586 Y A 0.1605
587 E A -1.6159
588 L A 0.2335
589 P A -0.0593
590 V A 0.2150
591 Q A -1.4068
592 D A -1.9724
593 A A 0.0000
594 V A 0.0000
595 K A -1.4269
596 Q A -0.7193
597 I A 0.0000
598 I A 0.0000
599 D A -1.7563
600 Y A -0.1388
601 L A 0.0000
602 D A -0.5162
603 T A -0.3501
604 K A -1.0823
605 G A -0.2809
606 Y A 0.1323
607 L A 0.0000
608 P A -0.0362
609 A A -0.2614
610 K A -2.0057
611 K A -2.3585
612 E A -2.4528
613 K A -1.8021
614 L A 0.9792
615 A A 0.2666
616 A A 0.0327
617 A A 0.2851
618 L A 1.0669
619 E A -1.7499
620 H A -1.4982
621 H A -1.3497
622 H A -1.3491
623 H A -1.3475
624 H A -1.3478
625 H A -1.1698
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Automated mutations analysis

In the automated mutations mode, the server selects aggregation prone resides and each selected residue is mutated to glutamic acid, lysine, aspartic acid and arginine. The table below shows 2 best scored mutants for each mutated residue. Protein variants are ordered according to the mutation effect they had on protein stability (energetic effect) together with the difference in the average per-residue aggregation score between the wild type and the mutant (in the table green values indicate a positive change, grey are neutral, and orange/red mean destabilizing or more aggregation prone mutants).
Summary for all the mutants can be found in this CSV file.

Mutant
Energetic effect
Score comparison
VR562A -0.8588 -0.0048 View CSV PDB
VE2A -0.6494 -0.0046 View CSV PDB
VR183A -0.6485 -0.0043 View CSV PDB
FR178A -0.7417 -0.0038 View CSV PDB
FD476A -0.304 -0.0058 View CSV PDB
VR2A -0.3741 -0.005 View CSV PDB
VK562A -0.4932 -0.0042 View CSV PDB
VE183A -0.2591 -0.0045 View CSV PDB
FE476A -0.043 -0.0056 View CSV PDB
FR408A 0.0667 -0.0056 View CSV PDB
FK408A 0.1186 -0.0051 View CSV PDB
FK178A 0.0966 -0.0037 View CSV PDB
 
Laboratory of Theory of Biopolymers 2018