Project name: d092b2fae0fe28d [mutate: AG9H, GR47H, QR48H, VA55H, AV80H, YF103H, AS105H, QA120H, LS123H]

Chain sequence(s)	H: QVQLQQPGAELVKPGASVKMSCKASGYTFTSYNMHWVKQTPGQGLEWIGVIYSGNGDTSYNQKFKGKATLTADKSSSTAYMQINSLTSEDSAVYYCARERDTRFGNWGQGTLVTVSA; L: NIVLTQSPASLAVSLGLRATISCRASESVDIYGNSFMHWYQQKPGQPPKLLIYLASNLESGVPARFSGSGSRTDFTLTIDPVEADDAATYYCQQNNEDPYTFGGGTKLEIK
Selected Chain(s)	H,L
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No
Mutated residues	AS105H,AG9H,LS123H,QA120H,GR47H,VA55H,AV80H,YF103H,QR48H
Energy difference between WT (input) and mutated protein (by FoldX)	1.332 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:02)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:02)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:02)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:02)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:02)
[INFO]       FoldX:    Building mutant model                                                       (00:01:20)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:05:37)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:06:49)
[INFO]       Main:     Simulation completed successfully.                                          (00:06:51)

• Minimal score value: -3.0738
• Maximal score value: 1.1396
• Average score: -0.7295
• Total score value: -166.3246

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	Q	H	-1.3332
2	V	H	-0.8473
3	Q	H	-1.3167
4	L	H	0.0000
5	Q	H	-1.3431
6	Q	H	-1.0050
7	P	H	-1.0265
8	G	H	-1.0465
9	G	H	-0.8567	mutated: AG9H
11	E	H	-0.7113
12	L	H	0.6104
13	V	H	-0.4349
14	K	H	-1.6230
15	P	H	-1.3905
16	G	H	-1.1816
17	A	H	-0.9893
18	S	H	-1.0712
19	V	H	0.0000
20	K	H	-1.2816
21	M	H	0.0000
22	S	H	-0.6201
23	C	H	0.0000
24	K	H	-1.3723
25	A	H	0.0000
26	S	H	-0.9229
27	G	H	-0.8294
28	Y	H	-0.2879
29	T	H	-0.1812
30	F	H	0.0000
35	T	H	-0.5994
36	S	H	-0.5197
37	Y	H	-0.7185
38	N	H	-0.9459
39	M	H	0.0000
40	H	H	0.0000
41	W	H	0.0000
42	V	H	0.0000
43	K	H	0.0000
44	Q	H	-0.8393
45	T	H	-1.6304
46	P	H	-1.7072
47	R	H	-2.9484	mutated: GR47H
48	R	H	-3.0431	mutated: QR48H
49	G	H	-1.8340
50	L	H	0.0000
51	E	H	-1.0257
52	W	H	0.0000
53	I	H	0.0000
54	G	H	0.0000
55	A	H	0.0000	mutated: VA55H
56	I	H	0.0000
57	Y	H	-0.4782
58	S	H	0.0000
59	G	H	-1.1764
62	N	H	-1.8605
63	G	H	-1.7315
64	D	H	-2.1489
65	T	H	-0.9819
66	S	H	-0.9081
67	Y	H	-1.2558
68	N	H	0.0000
69	Q	H	-2.9539
70	K	H	-2.8802
71	F	H	0.0000
72	K	H	-2.8335
74	G	H	-1.9263
75	K	H	-1.6783
76	A	H	0.0000
77	T	H	-0.7316
78	L	H	0.0000
79	T	H	-0.3749
80	V	H	-0.3293	mutated: AV80H
81	D	H	-1.0930
82	K	H	-1.3409
83	S	H	-0.8566
84	S	H	-0.7822
85	S	H	-0.8661
86	T	H	0.0000
87	A	H	0.0000
88	Y	H	-0.2181
89	M	H	0.0000
90	Q	H	-0.8956
91	I	H	0.0000
92	N	H	-0.9498
93	S	H	-0.9544
94	L	H	0.0000
95	T	H	-1.3005
96	S	H	-1.4473
97	E	H	-1.9701
98	D	H	0.0000
99	S	H	-0.6111
100	A	H	0.0000
101	V	H	0.1351
102	Y	H	0.0000
103	F	H	0.0000	mutated: YF103H
104	C	H	0.0000
105	S	H	0.0000	mutated: AS105H
106	R	H	0.0000
107	E	H	-1.7735
108	R	H	-3.0738
109	D	H	-2.8363
113	T	H	-1.5223
114	R	H	-1.4806
115	F	H	0.0000
116	G	H	-1.4854
117	N	H	-0.9354
118	W	H	-0.6072
119	G	H	0.0000
120	A	H	-0.6423	mutated: QA120H
121	G	H	0.0000
122	T	H	0.0000
123	S	H	-0.3183	mutated: LS123H
124	V	H	0.0000
125	T	H	-0.3068
126	V	H	0.0000
127	S	H	-0.4209
128	A	H	-0.4371
1	N	L	-1.6261
2	I	L	0.0000
3	V	L	0.7869
4	L	L	0.0000
5	T	L	-0.6505
6	Q	L	-0.7012
7	S	L	-0.6436
8	P	L	-0.4621
9	A	L	-0.5459
10	S	L	-0.9597
11	L	L	-0.5068
12	A	L	-0.8658
13	V	L	0.0000
14	S	L	-0.4650
15	L	L	-0.0097
16	G	L	-0.4522
17	L	L	-0.0459
18	R	L	-1.9576
19	A	L	0.0000
20	T	L	-0.7491
21	I	L	0.0000
22	S	L	-0.9125
23	C	L	0.0000
24	R	L	-2.5897
25	A	L	0.0000
26	S	L	-1.2099
27	E	L	-2.1053
28	S	L	-1.9246
29	V	L	0.0000
30	D	L	-0.9854
31	I	L	0.5052
34	Y	L	1.1396
35	G	L	-0.0926
36	N	L	-0.6926
37	S	L	-0.7535
38	F	L	-0.1957
39	M	L	0.0000
40	H	L	0.0000
41	W	L	0.0000
42	Y	L	0.0000
43	Q	L	0.0000
44	Q	L	-1.2636
45	K	L	-1.6348
46	P	L	-1.0965
47	G	L	-1.2955
48	Q	L	-1.8338
49	P	L	-1.0809
50	P	L	0.0000
51	K	L	-1.5170
52	L	L	0.0000
53	L	L	0.0000
54	I	L	0.0000
55	Y	L	-0.1507
56	L	L	-0.3118
57	A	L	0.0000
65	S	L	-0.4616
66	N	L	-0.4534
67	L	L	0.0284
68	E	L	-0.5559
69	S	L	-0.5662
70	G	L	-0.5710
71	V	L	0.0000
72	P	L	-0.2816
74	A	L	-0.3805
75	R	L	-1.2939
76	F	L	0.0000
77	S	L	-0.5654
78	G	L	-0.3525
79	S	L	-0.7401
80	G	L	-1.4292
83	S	L	-2.0038
84	R	L	-2.9152
85	T	L	-2.6017
86	D	L	-2.6178
87	F	L	0.0000
88	T	L	-0.7976
89	L	L	0.0000
90	T	L	-1.0110
91	I	L	0.0000
92	D	L	-2.4104
93	P	L	-1.6441
94	V	L	0.0000
95	E	L	-1.8306
96	A	L	-1.5170
97	D	L	-2.1332
98	D	L	0.0000
99	A	L	-1.6584
100	A	L	0.0000
101	T	L	-1.1233
102	Y	L	0.0000
103	Y	L	0.0000
104	C	L	0.0000
105	Q	L	0.0000
106	Q	L	0.0000
107	N	L	-0.6172
108	N	L	-0.9707
109	E	L	-1.6050
114	D	L	-2.1572
115	P	L	0.0000
116	Y	L	-0.6283
117	T	L	-0.2628
118	F	L	0.0000
119	G	L	0.0000
120	G	L	-1.1214
121	G	L	-0.7437
122	T	L	0.0000
123	K	L	-1.9143
124	L	L	0.0000
125	E	L	-1.9623
126	I	L	-1.1348
127	K	L	-1.3508