Aggrescan4D: 2fea1a93d2639e8

Project name: 2fea1a93d2639e8

Status: done

Started: 2026-08-12 08:38:31

Chain sequence(s)	A: EVQLVESGGGLVQPGGSLRLSCAASGFSFSDFWMSWVRQAPGKGLEWVATISQAGSEKDYVDSVKGRFTISRDNAKKSLYLQMNSLRAEDTAVYYCASGVYSFDYRGQGTLVTVSSSGGGGSEVQLVESGGALVKPGGSLRLSCAASGFTFTEAWMHWVRQAPGKQLEWVAQIKDRSQTYATYYAESVKGRFTISRDDSKNTLYLQMNSLKTEDTAVYYCRGVYYANAPFDYWGQGTLVTVSSGGGGSGGGGSGGGGSDIVMTQSPLSLPVTPGEPASISCRSSQSLVHNTGNTYLSWYLQKPGQSPQSLIYKVSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCGQGTQAPFTFGSGTKVEIKGGGGDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLYITREPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALAAPIEKTISKAKGQPREPQVYTLPPCREEMTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK B: EVQLLESGGGLVQPGGSLRLSCAASGFTFDDYAIGWFRQAPGKEREGVSCIRVSDGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAAGSLYTCVQSIVWPARPYYDMDYWGQGTLVTVSSEPKSSDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLYITREPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALAAPIEKTISKAKGQPREPQVCTLPPSREEMTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK input PDB
Selected Chain(s)	A,B
Distance of aggregation	10 Å
FoldX usage	Yes
pH calculations	No
alphaCutter usage	No
Dynamic mode	No
Automated mutations	Yes
Downloads	Download all the data

Simulation log

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[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:01)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:01)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:01)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:01)
[INFO]       FoldX:    Starting FoldX energy minimization                                          (00:00:02)
[INFO]       Analysis: Starting Aggrescan4D on folded.pdb                                          (00:11:20)
[INFO]       AutoMut:  Residue number 238 from chain A and a score of 1.737 (leucine) selected for 
                       automated mutation                                                          (00:11:28)
[INFO]       AutoMut:  Residue number 126 from chain B and a score of 1.717 (leucine) selected for 
                       automated mutation                                                          (00:11:28)
[INFO]       AutoMut:  Residue number 11 from chain B and a score of 1.515 (leucine) selected for  
                       automated mutation                                                          (00:11:28)
[INFO]       AutoMut:  Residue number 133 from chain A and a score of 1.456 (leucine) selected for 
                       automated mutation                                                          (00:11:28)
[INFO]       AutoMut:  Residue number 407 from chain A and a score of 1.405 (isoleucine) selected  
                       for automated mutation                                                      (00:11:28)
[INFO]       AutoMut:  Residue number 267 from chain A and a score of 1.253 (leucine) selected for 
                       automated mutation                                                          (00:11:28)
[INFO]       AutoMut:  Mutating residue number 238 from chain A (leucine) into glutamic acid       (00:11:28)
[INFO]       AutoMut:  Mutating residue number 238 from chain A (leucine) into aspartic acid       (00:11:28)
[INFO]       AutoMut:  Mutating residue number 126 from chain B (leucine) into glutamic acid       (00:11:28)
[INFO]       AutoMut:  Mutating residue number 238 from chain A (leucine) into arginine            (00:11:49)
[INFO]       AutoMut:  Mutating residue number 238 from chain A (leucine) into lysine              (00:11:53)
[INFO]       AutoMut:  Mutating residue number 126 from chain B (leucine) into lysine              (00:11:57)
[INFO]       AutoMut:  Mutating residue number 126 from chain B (leucine) into aspartic acid       (00:12:15)
[INFO]       AutoMut:  Mutating residue number 11 from chain B (leucine) into glutamic acid        (00:12:28)
[INFO]       AutoMut:  Mutating residue number 126 from chain B (leucine) into arginine            (00:12:39)
[INFO]       AutoMut:  Mutating residue number 11 from chain B (leucine) into aspartic acid        (00:12:42)
[INFO]       AutoMut:  Mutating residue number 11 from chain B (leucine) into lysine               (00:12:52)
[INFO]       AutoMut:  Mutating residue number 11 from chain B (leucine) into arginine             (00:13:03)
[INFO]       AutoMut:  Mutating residue number 133 from chain A (leucine) into glutamic acid       (00:13:17)
[INFO]       AutoMut:  Mutating residue number 133 from chain A (leucine) into aspartic acid       (00:13:32)
[INFO]       AutoMut:  Mutating residue number 407 from chain A (isoleucine) into glutamic acid    (00:13:32)
[INFO]       AutoMut:  Mutating residue number 133 from chain A (leucine) into lysine              (00:13:41)
[INFO]       AutoMut:  Mutating residue number 133 from chain A (leucine) into arginine            (00:13:53)
[INFO]       AutoMut:  Mutating residue number 407 from chain A (isoleucine) into lysine           (00:13:56)
[INFO]       AutoMut:  Mutating residue number 407 from chain A (isoleucine) into aspartic acid    (00:14:04)
[INFO]       AutoMut:  Mutating residue number 267 from chain A (leucine) into glutamic acid       (00:14:20)
[INFO]       AutoMut:  Mutating residue number 267 from chain A (leucine) into aspartic acid       (00:14:20)
[INFO]       AutoMut:  Mutating residue number 407 from chain A (isoleucine) into arginine         (00:14:23)
[INFO]       AutoMut:  Mutating residue number 267 from chain A (leucine) into arginine            (00:14:39)
[INFO]       AutoMut:  Mutating residue number 267 from chain A (leucine) into lysine              (00:14:40)
[INFO]       AutoMut:  Effect of mutation residue number 238 from chain A (leucine) into glutamic  
                       acid: Energy difference: 1.0196 kcal/mol, Difference in average score from  
                       the base case: -0.0127                                                      (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 238 from chain A (leucine) into lysine:   
                       Energy difference: -0.1524 kcal/mol, Difference in average score from the   
                       base case: -0.0132                                                          (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 238 from chain A (leucine) into aspartic  
                       acid: Energy difference: 1.4604 kcal/mol, Difference in average score from  
                       the base case: -0.0117                                                      (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 238 from chain A (leucine) into arginine: 
                       Energy difference: -0.2378 kcal/mol, Difference in average score from the   
                       base case: -0.0138                                                          (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 126 from chain B (leucine) into glutamic  
                       acid: Energy difference: 0.3020 kcal/mol, Difference in average score from  
                       the base case: -0.0107                                                      (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 126 from chain B (leucine) into lysine:   
                       Energy difference: -0.0936 kcal/mol, Difference in average score from the   
                       base case: -0.0107                                                          (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 126 from chain B (leucine) into aspartic  
                       acid: Energy difference: 0.9078 kcal/mol, Difference in average score from  
                       the base case: -0.0104                                                      (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 126 from chain B (leucine) into arginine: 
                       Energy difference: -1.0289 kcal/mol, Difference in average score from the   
                       base case: -0.0098                                                          (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain B (leucine) into glutamic   
                       acid: Energy difference: 0.7967 kcal/mol, Difference in average score from  
                       the base case: -0.0176                                                      (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain B (leucine) into lysine:    
                       Energy difference: -0.0505 kcal/mol, Difference in average score from the   
                       base case: -0.0123                                                          (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain B (leucine) into aspartic   
                       acid: Energy difference: 0.9538 kcal/mol, Difference in average score from  
                       the base case: -0.0175                                                      (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain B (leucine) into arginine:  
                       Energy difference: -0.0904 kcal/mol, Difference in average score from the   
                       base case: -0.0118                                                          (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 133 from chain A (leucine) into glutamic  
                       acid: Energy difference: 1.1177 kcal/mol, Difference in average score from  
                       the base case: -0.0120                                                      (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 133 from chain A (leucine) into lysine:   
                       Energy difference: 0.1858 kcal/mol, Difference in average score from the    
                       base case: -0.0117                                                          (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 133 from chain A (leucine) into aspartic  
                       acid: Energy difference: 1.3885 kcal/mol, Difference in average score from  
                       the base case: -0.0118                                                      (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 133 from chain A (leucine) into arginine: 
                       Energy difference: -0.0239 kcal/mol, Difference in average score from the   
                       base case: -0.0121                                                          (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 407 from chain A (isoleucine) into        
                       glutamic acid: Energy difference: -0.2952 kcal/mol, Difference in average   
                       score from the base case: -0.0125                                           (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 407 from chain A (isoleucine) into        
                       lysine: Energy difference: -0.1938 kcal/mol, Difference in average score    
                       from the base case: -0.0102                                                 (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 407 from chain A (isoleucine) into        
                       aspartic acid: Energy difference: 0.2481 kcal/mol, Difference in average    
                       score from the base case: -0.0119                                           (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 407 from chain A (isoleucine) into        
                       arginine: Energy difference: -0.5705 kcal/mol, Difference in average score  
                       from the base case: -0.0113                                                 (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 267 from chain A (leucine) into glutamic  
                       acid: Energy difference: -0.4981 kcal/mol, Difference in average score from 
                       the base case: -0.0114                                                      (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 267 from chain A (leucine) into lysine:   
                       Energy difference: 0.2064 kcal/mol, Difference in average score from the    
                       base case: -0.0111                                                          (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 267 from chain A (leucine) into aspartic  
                       acid: Energy difference: -0.2036 kcal/mol, Difference in average score from 
                       the base case: -0.0114                                                      (00:15:07)
[INFO]       AutoMut:  Effect of mutation residue number 267 from chain A (leucine) into arginine: 
                       Energy difference: 0.3008 kcal/mol, Difference in average score from the    
                       base case: -0.0114                                                          (00:15:07)
[INFO]       Main:     Simulation completed successfully.                                          (00:15:15)

Note Your prediction results will be deleted after 360 days.

Drag cursor over the plot to display residue labels.

Show buried residues

Minimal score value: -3.7673
Maximal score value: 1.7372
Average score: -0.8129
Total score value: -783.6657

Download the table (csv)

The table below lists A4D score for protein residues. Residues with A4D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan4D score

1	E	A	-2.1524
2	V	A	-1.6347
3	Q	A	-1.6193
4	L	A	0.0000
5	V	A	0.4240
6	E	A	0.0000
7	S	A	-0.5031
8	G	A	-1.0246
9	G	A	-0.6116
10	G	A	-0.1129
11	L	A	0.1562
12	V	A	-0.4021
13	Q	A	-1.5370
14	P	A	-1.6709
15	G	A	-1.3859
16	G	A	-1.0373
17	S	A	-1.2869
18	L	A	-1.1783
19	R	A	-2.1760
20	L	A	0.0000
21	S	A	-0.4886
22	C	A	0.0000
23	A	A	-0.5175
24	A	A	0.0000
25	S	A	-1.3331
26	G	A	-1.3143
27	F	A	-0.8228
28	S	A	-0.8838
29	F	A	0.0000
30	S	A	-1.4527
31	D	A	-1.7553
32	F	A	-0.5271
33	W	A	-0.0473
34	M	A	0.0000
35	S	A	0.0000
36	W	A	0.0000
37	V	A	0.0000
38	R	A	0.0000
39	Q	A	-1.1585
40	A	A	-1.4251
41	P	A	-1.3856
42	G	A	-1.8116
43	K	A	-2.2687
44	G	A	-1.8786
45	L	A	-1.2985
46	E	A	-1.2689
47	W	A	-0.1634
48	V	A	0.0000
49	A	A	0.0000
50	T	A	0.0000
51	I	A	0.0000
52	S	A	0.0000
53	Q	A	-1.2114
54	A	A	-0.9419
55	G	A	-1.2001
56	S	A	-1.4019
57	E	A	-2.6658
58	K	A	-2.3482
59	D	A	-2.3536
60	Y	A	-1.4975
61	V	A	-1.2406
62	D	A	-2.7112
63	S	A	-1.5911
64	V	A	0.0000
65	K	A	-2.6491
66	G	A	-1.6491
67	R	A	0.0000
68	F	A	0.0000
69	T	A	-1.0705
70	I	A	0.0000
71	S	A	-0.6642
72	R	A	-1.0949
73	D	A	-1.6499
74	N	A	-2.1707
75	A	A	-1.6012
76	K	A	-2.6774
77	K	A	-2.5974
78	S	A	-1.4425
79	L	A	0.0000
80	Y	A	-0.5395
81	L	A	0.0000
82	Q	A	-1.2555
83	M	A	0.0000
84	N	A	-1.4710
85	S	A	-1.2298
86	L	A	0.0000
87	R	A	-2.1404
88	A	A	-1.6191
89	E	A	-2.1669
90	D	A	0.0000
91	T	A	-0.7844
92	A	A	0.0000
93	V	A	0.0000
94	Y	A	0.0000
95	Y	A	0.0000
96	C	A	0.0000
97	A	A	0.0000
98	S	A	0.0000
99	G	A	0.1660
100	V	A	0.9691
101	Y	A	0.8823
102	S	A	0.2313
103	F	A	-0.1327
104	D	A	-1.3413
105	Y	A	-0.9434
106	R	A	-1.3944
107	G	A	-0.8799
108	Q	A	-1.5472
109	G	A	0.0000
110	T	A	-0.2962
111	L	A	0.0000
112	V	A	0.0000
113	T	A	0.0000
114	V	A	0.0000
115	S	A	-1.3319
116	S	A	-1.3286
117	S	A	-1.4723
118	G	A	-1.4633
119	G	A	-1.3521
120	G	A	-1.3258
121	G	A	-1.3898
122	S	A	-1.3982
123	E	A	-1.9810
124	V	A	-0.5686
125	Q	A	-0.3561
126	L	A	0.0000
127	V	A	0.0000
128	E	A	0.0000
129	S	A	0.0000
130	G	A	-0.7944
131	G	A	0.2958
132	A	A	0.7362
133	L	A	1.4565
134	V	A	-0.1862
135	K	A	-1.8477
136	P	A	-1.8329
137	G	A	-1.4729
138	G	A	-1.1002
139	S	A	-1.3948
140	L	A	-0.9166
141	R	A	-2.2028
142	L	A	0.0000
143	S	A	-0.7117
144	C	A	0.0000
145	A	A	-0.5231
146	A	A	0.0000
147	S	A	-0.5091
148	G	A	-0.7252
149	F	A	-0.2785
150	T	A	-0.2815
151	F	A	0.0000
152	T	A	-1.3392
153	E	A	-1.3568
154	A	A	0.0000
155	W	A	0.0484
156	M	A	0.0000
157	H	A	0.0000
158	W	A	0.0000
159	V	A	0.0000
160	R	A	0.0000
161	Q	A	-0.7675
162	A	A	-1.1234
163	P	A	-0.8982
164	G	A	-1.5695
165	K	A	-2.4368
166	Q	A	-1.7603
167	L	A	0.0000
168	E	A	-1.0887
169	W	A	0.0000
170	V	A	0.0000
171	A	A	0.0000
172	Q	A	0.0000
173	I	A	0.0000
174	K	A	-0.6309
175	D	A	-1.5176
176	R	A	-2.3436
177	S	A	-1.5904
178	Q	A	-1.4021
179	T	A	-0.9846
180	Y	A	-0.5124
181	A	A	-0.2182
182	T	A	0.2801
183	Y	A	0.2269
184	Y	A	-0.5883
185	A	A	0.0000
186	E	A	-2.5143
187	S	A	-1.7927
188	V	A	0.0000
189	K	A	-2.3679
190	G	A	-1.6820
191	R	A	-1.6067
192	F	A	0.0000
193	T	A	-0.7743
194	I	A	0.0000
195	S	A	-0.2507
196	R	A	-0.9449
197	D	A	-1.2876
198	D	A	-1.6803
199	S	A	-1.4183
200	K	A	-2.2577
201	N	A	-1.5978
202	T	A	-1.0701
203	L	A	0.0000
204	Y	A	-0.5000
205	L	A	0.0000
206	Q	A	-1.2958
207	M	A	0.0000
208	N	A	-1.5327
209	S	A	-1.3264
210	L	A	0.0000
211	K	A	-2.2102
212	T	A	-1.8413
213	E	A	-2.2624
214	D	A	0.0000
215	T	A	-0.3394
216	A	A	0.0000
217	V	A	0.8727
218	Y	A	0.0000
219	Y	A	0.0000
220	C	A	0.0000
221	R	A	0.0000
222	G	A	0.0000
223	V	A	0.5504
224	Y	A	0.3576
225	Y	A	0.2252
226	A	A	-0.4322
227	N	A	-1.1146
228	A	A	-0.4529
229	P	A	-0.1959
230	F	A	0.4465
231	D	A	0.0000
232	Y	A	0.4779
233	W	A	0.0305
234	G	A	0.0000
235	Q	A	-0.9666
236	G	A	-0.0292
237	T	A	0.5984
238	L	A	1.7372
239	V	A	0.0000
240	T	A	0.3744
241	V	A	0.0000
242	S	A	-0.9857
243	S	A	-1.4415
244	G	A	-1.3109
245	G	A	-1.1484
246	G	A	-1.1665
247	G	A	-1.2155
248	S	A	-1.0264
249	G	A	-1.3935
250	G	A	-1.1949
251	G	A	-1.1780
252	G	A	-1.1875
253	S	A	-1.0273
254	G	A	-1.1901
255	G	A	-1.3820
256	G	A	-1.2798
257	G	A	-1.5050
258	S	A	-1.0039
259	D	A	-1.1860
260	I	A	0.0000
261	V	A	0.8683
262	M	A	0.0000
263	T	A	-0.3492
264	Q	A	0.0000
265	S	A	-0.1071
266	P	A	0.4133
267	L	A	1.2530
268	S	A	0.3977
269	L	A	0.0783
270	P	A	-0.9095
271	V	A	0.0000
272	T	A	-1.5588
273	P	A	-1.8200
274	G	A	-2.0682
275	E	A	-2.8767
276	P	A	-2.4203
277	A	A	0.0000
278	S	A	-0.7515
279	I	A	0.0000
280	S	A	-0.9193
281	C	A	0.0000
282	R	A	-2.3543
283	S	A	0.0000
284	S	A	-0.9360
285	Q	A	-1.5399
286	S	A	-0.8443
287	L	A	0.0000
288	V	A	0.3235
289	H	A	-0.6978
290	N	A	-1.3372
291	T	A	-0.8827
292	G	A	-0.7875
293	N	A	-0.5994
294	T	A	-0.1453
295	Y	A	-0.0546
296	L	A	0.0000
297	S	A	0.0000
298	W	A	0.0000
299	Y	A	0.0000
300	L	A	0.0000
301	Q	A	-0.9832
302	K	A	-1.6810
303	P	A	-1.2072
304	G	A	-1.4332
305	Q	A	-1.8010
306	S	A	-1.1650
307	P	A	0.0000
308	Q	A	-1.1757
309	S	A	0.0000
310	L	A	0.0000
311	I	A	0.0000
312	Y	A	-0.7144
313	K	A	-0.7552
314	V	A	0.0000
315	S	A	-1.2710
316	N	A	-1.9349
317	R	A	-2.2773
318	A	A	-1.1312
319	S	A	-0.7908
320	G	A	-0.9277
321	V	A	-1.2184
322	P	A	-1.4082
323	D	A	-2.5026
324	R	A	-2.1899
325	F	A	0.0000
326	S	A	-1.6538
327	G	A	0.0000
328	S	A	-1.0330
329	G	A	-1.0626
330	S	A	-0.7722
331	G	A	-0.7494
332	T	A	-1.5291
333	D	A	-2.2316
334	F	A	0.0000
335	T	A	-1.1378
336	L	A	0.0000
337	K	A	-1.8787
338	I	A	0.0000
339	S	A	-2.4640
340	R	A	-3.4345
341	V	A	0.0000
342	E	A	-2.2594
343	A	A	-1.1916
344	E	A	-2.1371
345	D	A	0.0000
346	V	A	-0.8585
347	G	A	0.0000
348	V	A	-0.1615
349	Y	A	0.0000
350	Y	A	0.0000
351	C	A	0.0000
352	G	A	0.0000
353	Q	A	0.0000
354	G	A	-0.5417
355	T	A	0.0000
356	Q	A	-1.2932
357	A	A	-0.6062
358	P	A	-0.8557
359	F	A	0.0000
360	T	A	-0.1529
361	F	A	0.0000
362	G	A	0.0000
363	S	A	-0.3348
364	G	A	0.0000
365	T	A	0.0000
366	K	A	-0.2498
367	V	A	0.0000
368	E	A	-0.8759
369	I	A	-0.7821
370	K	A	-2.1644
371	G	A	-1.9639
372	G	A	-1.5467
373	G	A	-2.4410
374	G	A	-2.3668
375	D	A	-3.2952
376	K	A	-3.1885
377	T	A	-1.9492
378	H	A	-1.8665
379	T	A	-0.7690
380	C	A	-0.0850
381	P	A	-0.1712
382	P	A	0.0446
383	C	A	0.3906
384	P	A	-0.4395
385	A	A	-0.5751
386	P	A	-1.0775
387	E	A	-2.0186
388	A	A	-1.1511
389	A	A	-0.8345
390	G	A	-0.8728
391	G	A	-0.5332
392	P	A	0.0000
393	S	A	0.0000
394	V	A	0.0000
395	F	A	0.0000
396	L	A	0.0000
397	F	A	-0.1271
398	P	A	-0.6368
399	P	A	0.0000
400	K	A	-2.5712
401	P	A	-1.5728
402	K	A	-1.2501
403	D	A	0.0000
404	T	A	0.0000
405	L	A	0.0000
406	Y	A	0.4510
407	I	A	1.4046
408	T	A	0.0708
409	R	A	-1.5327
410	E	A	-2.2840
411	P	A	0.0000
412	E	A	-1.6848
413	V	A	0.0000
414	T	A	-0.3389
415	C	A	0.0000
416	V	A	0.0000
417	V	A	0.0000
418	V	A	0.0000
419	D	A	-1.0001
420	V	A	0.0000
421	S	A	-1.9369
422	H	A	-2.5574
423	E	A	-2.9891
424	D	A	-2.6472
425	P	A	-2.6721
426	E	A	-3.1427
427	V	A	-2.0289
428	K	A	-2.2396
429	F	A	-1.1520
430	N	A	-1.0826
431	W	A	0.0000
432	Y	A	-0.7204
433	V	A	-1.0582
434	D	A	-2.3213
435	G	A	-0.9778
436	V	A	0.5036
437	E	A	-0.7387
438	V	A	-0.6130
439	H	A	-1.8994
440	N	A	-2.1836
441	A	A	-1.8766
442	K	A	-2.4006
443	T	A	-1.8740
444	K	A	-2.1296
445	P	A	-2.2771
446	R	A	-3.4338
447	E	A	-3.4239
448	E	A	-3.2384
449	Q	A	-1.5266
450	Y	A	0.0755
451	N	A	-0.9375
452	S	A	-1.1060
453	T	A	-1.8224
454	Y	A	0.0000
455	R	A	-2.2183
456	V	A	0.0000
457	V	A	0.0000
458	S	A	0.0000
459	V	A	0.0000
460	L	A	0.0000
461	T	A	-1.1237
462	V	A	0.0000
463	L	A	0.1736
464	H	A	-0.4618
465	Q	A	-1.2541
466	D	A	-1.6608
467	W	A	0.0000
468	L	A	-1.0818
469	N	A	-2.2395
470	G	A	-2.3942
471	K	A	-2.5854
472	E	A	-2.7621
473	Y	A	0.0000
474	K	A	-1.4256
475	C	A	0.0000
476	K	A	-1.1355
477	V	A	0.0000
478	S	A	-1.4039
479	N	A	0.0000
480	K	A	-2.5033
481	A	A	-1.3400
482	L	A	-0.4403
483	A	A	-0.3166
484	A	A	-0.1850
485	P	A	-0.6646
486	I	A	-0.2275
487	E	A	-0.8777
488	K	A	0.0000
489	T	A	-0.8683
490	I	A	0.0000
491	S	A	-1.7411
492	K	A	-1.5998
493	A	A	-1.3979
494	K	A	-2.5613
495	G	A	-2.2265
496	Q	A	-2.1109
497	P	A	-1.7379
498	R	A	-1.9903
499	E	A	-2.5439
500	P	A	0.0000
501	Q	A	-1.2631
502	V	A	0.0000
503	Y	A	0.0000
504	T	A	-0.7274
505	L	A	0.0000
506	P	A	-0.3090
507	P	A	-0.5856
508	C	A	-0.9922
509	R	A	-2.0061
510	E	A	-1.5238
511	E	A	0.0000
512	M	A	-1.5124
513	T	A	-1.3806
514	K	A	-2.2103
515	N	A	-2.5450
516	Q	A	-2.2586
517	V	A	0.0000
518	S	A	0.0000
519	L	A	0.0000
520	W	A	0.0000
521	C	A	0.0000
522	L	A	0.0000
523	V	A	0.0000
524	K	A	-0.5631
525	G	A	-1.0460
526	F	A	0.0000
527	Y	A	-1.1684
528	P	A	0.0000
529	S	A	-0.4603
530	D	A	-1.6431
531	I	A	0.0000
532	A	A	0.0000
533	V	A	0.0000
534	E	A	-1.1792
535	W	A	0.0000
536	E	A	-1.7417
537	S	A	0.0000
538	N	A	-1.8819
539	G	A	-1.7674
540	Q	A	-2.2488
541	P	A	-1.8748
542	E	A	0.0000
543	N	A	-1.9364
544	N	A	-1.2868
545	Y	A	-0.8604
546	K	A	-1.1137
547	T	A	-0.7011
548	T	A	0.0000
549	P	A	0.0000
550	P	A	-0.3254
551	V	A	-0.0214
552	L	A	-0.1235
553	D	A	-0.9264
554	S	A	-1.3270
555	D	A	-2.0488
556	G	A	-1.1470
557	S	A	0.0000
558	F	A	-0.2013
559	F	A	0.0000
560	L	A	0.0000
561	Y	A	0.0000
562	S	A	0.0000
563	K	A	0.0000
564	L	A	0.0000
565	T	A	-0.7807
566	V	A	0.0000
567	D	A	-2.2971
568	K	A	-2.5969
569	S	A	-2.1906
570	R	A	-1.9846
571	W	A	0.0000
572	Q	A	-2.3695
573	Q	A	-2.1796
574	G	A	-1.2617
575	N	A	-0.9116
576	V	A	-0.0081
577	F	A	0.0000
578	S	A	0.0000
579	C	A	0.0000
580	S	A	0.0000
581	V	A	0.0000
582	M	A	-0.4831
583	H	A	0.0000
584	E	A	-1.1106
585	A	A	-1.5262
586	L	A	0.0000
587	H	A	-1.8192
588	N	A	-1.6460
589	H	A	-1.1417
590	Y	A	-0.4008
591	T	A	-0.5823
592	Q	A	-0.7948
593	K	A	-0.8332
594	S	A	-0.4437
595	L	A	0.0000
596	S	A	-0.2247
597	L	A	-0.3808
598	S	A	-0.6277
599	P	A	-1.2249
600	G	A	-1.4168
601	K	A	-2.0277
1	E	B	-2.3076
2	V	B	-0.9692
3	Q	B	-0.7858
4	L	B	0.0000
5	L	B	0.0000
6	E	B	0.0000
7	S	B	0.0000
8	G	B	0.0000
9	G	B	0.0000
10	G	B	0.9159
11	L	B	1.5153
12	V	B	0.1446
13	Q	B	-0.8843
14	P	B	-1.5800
15	G	B	-1.1610
16	G	B	-0.8365
17	S	B	-0.7344
18	L	B	-0.0622
19	R	B	-0.5203
20	L	B	0.0000
21	S	B	0.0000
22	C	B	0.0000
23	A	B	-0.4593
24	A	B	0.0000
25	S	B	-0.8935
26	G	B	-1.1570
27	F	B	-0.6801
28	T	B	-0.7291
29	F	B	0.0000
30	D	B	-2.5681
31	D	B	-1.8917
32	Y	B	-0.9753
33	A	B	0.0000
34	I	B	0.0000
35	G	B	0.0000
36	W	B	0.0000
37	F	B	0.0000
38	R	B	0.0000
39	Q	B	-1.0242
40	A	B	-1.4402
41	P	B	-1.6146
42	G	B	-1.7689
43	K	B	-2.2665
44	E	B	-2.0739
45	R	B	-1.4283
46	E	B	-1.2023
47	G	B	0.0000
48	V	B	0.0000
49	S	B	0.0000
50	C	B	0.0000
51	I	B	0.0000
52	R	B	-1.2742
53	V	B	0.0000
54	S	B	-2.0884
55	D	B	-2.3302
56	G	B	-1.5965
57	S	B	0.0000
58	T	B	-0.4356
59	Y	B	0.0000
60	Y	B	0.0000
61	A	B	0.0000
62	D	B	-0.5485
63	S	B	-0.6523
64	V	B	0.0000
65	K	B	-0.7291
66	G	B	-1.0375
67	R	B	0.0000
68	F	B	0.0000
69	T	B	-0.3113
70	I	B	0.0000
71	S	B	-0.8217
72	R	B	-1.6720
73	D	B	-1.6448
74	N	B	-1.7276
75	S	B	-0.9921
76	K	B	-1.3711
77	N	B	-1.6017
78	T	B	0.0000
79	L	B	0.0000
80	Y	B	0.0000
81	L	B	0.0000
82	Q	B	-0.4462
83	M	B	0.0000
84	N	B	-1.0152
85	S	B	-1.0239
86	L	B	0.0000
87	R	B	-2.1273
88	A	B	-1.6306
89	E	B	-2.2502
90	D	B	0.0000
91	T	B	-0.3835
92	A	B	0.0000
93	V	B	0.8442
94	Y	B	0.0000
95	Y	B	0.2302
96	C	B	0.0000
97	A	B	0.0000
98	A	B	0.0000
99	G	B	0.0000
100	S	B	-0.3692
101	L	B	0.1374
102	Y	B	0.1982
103	T	B	-0.1855
104	C	B	0.0000
105	V	B	0.0000
106	Q	B	-0.0126
107	S	B	0.0000
108	I	B	0.0000
109	V	B	0.3347
110	W	B	0.7465
111	P	B	-0.0075
112	A	B	-0.0082
113	R	B	-0.4168
114	P	B	-0.2927
115	Y	B	-0.1008
116	Y	B	0.6482
117	D	B	-0.7313
118	M	B	0.0000
119	D	B	-0.6683
120	Y	B	0.0000
121	W	B	0.1117
122	G	B	-0.2029
123	Q	B	-0.3435
124	G	B	0.0000
125	T	B	0.0000
126	L	B	1.7174
127	V	B	0.0000
128	T	B	0.5831
129	V	B	0.0000
130	S	B	-0.9922
131	S	B	-1.3618
132	E	B	-1.3383
133	P	B	-1.3153
134	K	B	-1.9021
135	S	B	-2.0826
136	S	B	-2.2877
137	D	B	-3.7673
138	K	B	-2.9885
139	T	B	-2.7637
140	H	B	-1.9983
141	T	B	-1.0800
142	C	B	-0.2435
143	P	B	-0.2676
144	P	B	-0.1099
145	C	B	0.0973
146	P	B	-0.6156
147	A	B	-0.7457
148	P	B	-1.1829
149	E	B	-2.0573
150	A	B	-1.1293
151	A	B	-0.9396
152	G	B	-0.9791
153	G	B	0.0000
154	P	B	0.0000
155	S	B	0.0000
156	V	B	0.0000
157	F	B	0.0000
158	L	B	0.0000
159	F	B	0.0000
160	P	B	-0.4058
161	P	B	0.0000
162	K	B	-1.9232
163	P	B	-1.3062
164	K	B	-1.3808
165	D	B	-1.6144
166	T	B	0.0000
167	L	B	0.0000
168	Y	B	0.2086
169	I	B	1.1675
170	T	B	-0.0785
171	R	B	-1.8343
172	E	B	-2.3641
173	P	B	0.0000
174	E	B	-1.7138
175	V	B	0.0000
176	T	B	-0.4503
177	C	B	0.0000
178	V	B	0.0000
179	V	B	0.0000
180	V	B	0.0000
181	D	B	-0.9403
182	V	B	0.0000
183	S	B	-2.0948
184	H	B	-2.5625
185	E	B	-2.9746
186	D	B	-2.5967
187	P	B	-2.6294
188	E	B	-3.1004
189	V	B	-1.9772
190	K	B	-2.2332
191	F	B	-1.3545
192	N	B	-1.3373
193	W	B	0.0000
194	Y	B	-1.0056
195	V	B	-1.1324
196	D	B	-2.3005
197	G	B	-1.1064
198	V	B	0.1756
199	E	B	-1.4725
200	V	B	-0.9096
201	H	B	-2.0568
202	N	B	-2.2557
203	A	B	-1.8843
204	K	B	-2.3822
205	T	B	-1.8340
206	K	B	-2.1067
207	P	B	-2.2707
208	R	B	-3.4144
209	E	B	-3.5416
210	E	B	-3.1758
211	Q	B	-1.3179
212	Y	B	0.3849
213	N	B	-0.2757
214	S	B	-0.8193
215	T	B	-1.6303
216	Y	B	0.0000
217	R	B	-2.0311
218	V	B	0.0000
219	V	B	-0.9922
220	S	B	0.0000
221	V	B	0.0000
222	L	B	0.0000
223	T	B	-1.0960
224	V	B	0.0000
225	L	B	0.4448
226	H	B	0.0000
227	Q	B	-0.9212
228	D	B	-1.3642
229	W	B	0.0000
230	L	B	-0.8963
231	N	B	-2.0173
232	G	B	-2.0428
233	K	B	-2.2386
234	E	B	-2.3580
235	Y	B	0.0000
236	K	B	-1.6689
237	C	B	0.0000
238	K	B	-1.4087
239	V	B	0.0000
240	S	B	-1.3808
241	N	B	0.0000
242	K	B	-2.4572
243	A	B	-1.3117
244	L	B	-0.3549
245	A	B	-0.1087
246	A	B	-0.2367
247	P	B	-0.7878
248	I	B	-0.5100
249	E	B	-1.8769
250	K	B	-1.1976
251	T	B	-1.3365
252	I	B	-0.6063
253	S	B	-1.2791
254	K	B	0.0000
255	A	B	-1.2895
256	K	B	-2.2918
257	G	B	-1.9594
258	Q	B	-2.1498
259	P	B	-1.8020
260	R	B	-2.2012
261	E	B	-2.6650
262	P	B	0.0000
263	Q	B	-0.9981
264	V	B	0.0000
265	C	B	0.0000
266	T	B	0.0000
267	L	B	0.0000
268	P	B	-0.3833
269	P	B	-0.8865
270	S	B	-1.5406
271	R	B	-2.7143
272	E	B	-2.8833
273	E	B	0.0000
274	M	B	-1.9649
275	T	B	-1.6918
276	K	B	-2.2637
277	N	B	-2.4821
278	Q	B	-2.2212
279	V	B	0.0000
280	S	B	0.0000
281	L	B	0.0000
282	S	B	0.0000
283	C	B	0.0000
284	A	B	0.0000
285	V	B	0.0000
286	K	B	-0.6977
287	G	B	-1.2516
288	F	B	0.0000
289	Y	B	-1.3821
290	P	B	0.0000
291	S	B	-0.7798
292	D	B	-1.5734
293	I	B	-0.7810
294	A	B	0.0000
295	V	B	0.0000
296	E	B	-1.2147
297	W	B	0.0000
298	E	B	-1.7891
299	S	B	0.0000
300	N	B	-1.8887
301	G	B	-1.7797
302	Q	B	-2.2604
303	P	B	-1.8822
304	E	B	0.0000
305	N	B	-1.9250
306	N	B	-1.2515
307	Y	B	-0.8600
308	K	B	-0.9883
309	T	B	-0.4258
310	T	B	0.0000
311	P	B	-0.1891
312	P	B	-0.2164
313	V	B	0.0144
314	L	B	-0.2317
315	D	B	-1.0326
316	S	B	-1.3563
317	D	B	-2.0863
318	G	B	-1.1778
319	S	B	0.0000
320	F	B	0.0000
321	F	B	0.0000
322	L	B	0.0000
323	V	B	0.0000
324	S	B	0.0000
325	K	B	0.0000
326	L	B	0.0000
327	T	B	-0.7803
328	V	B	0.0000
329	D	B	-2.2738
330	K	B	-2.5644
331	S	B	-2.1645
332	R	B	-1.9202
333	W	B	0.0000
334	Q	B	-2.3451
335	Q	B	-2.1503
336	G	B	-1.2334
337	N	B	-0.8704
338	V	B	-0.0023
339	F	B	0.0000
340	S	B	-0.9407
341	C	B	0.0000
342	S	B	0.0000
343	V	B	0.0000
344	M	B	0.0000
345	H	B	0.0000
346	E	B	-1.2260
347	A	B	-1.6062
348	L	B	0.0000
349	H	B	-1.8631
350	N	B	-1.4722
351	H	B	-1.0083
352	Y	B	-0.6070
353	T	B	0.0000
354	Q	B	-0.9959
355	K	B	-0.9214
356	S	B	-0.4983
357	L	B	0.0000
358	S	B	-0.2199
359	L	B	-0.3609
360	S	B	-0.6236
361	P	B	-1.2267
362	G	B	-1.4163
363	K	B	-2.0294

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Automated mutations analysis - charged mutations

In the automated mutations mode, the server selects aggregation prone resides and each selected residue is mutated to glutamic acid, lysine, aspartic acid and arginine. The table below shows 2 best scored mutants for each mutated residue. Protein variants are ordered according to the mutation effect they had on protein stability (energetic effect) together with the difference in the average per-residue aggregation score between the wild type and the mutant (in the table green values indicate a positive change, grey are neutral, and orange/red mean destabilizing or more aggregation prone mutants).
Summary for all the mutants can be found in this CSV file .

Mutant	Energetic effect	Score comparison
LR126B	-1.0289	-0.0098	View	CSV	PDB
IR407A	-0.5705	-0.0113	View	CSV	PDB
LE267A	-0.4981	-0.0114	View	CSV	PDB
LR238A	-0.2378	-0.0138	View	CSV	PDB
IE407A	-0.2952	-0.0125	View	CSV	PDB
LK238A	-0.1524	-0.0132	View	CSV	PDB
LD267A	-0.2036	-0.0114	View	CSV	PDB
LR11B	-0.0904	-0.0118	View	CSV	PDB
LK11B	-0.0505	-0.0123	View	CSV	PDB
LK126B	-0.0936	-0.0107	View	CSV	PDB
LR133A	-0.0239	-0.0121	View	CSV	PDB
LK133A	0.1858	-0.0117	View	CSV	PDB

Project name: 2fea1a93d2639e8

Status: done

Started: 2026-08-12 08:38:31

Automated mutations analysis - charged mutations

Mutant

Energetic effect

Score comparison