| Chain sequence(s) |
A: MGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETCLRTIPSHAFSNLPNISRIYVSIDVTLQQLESHSFYNLSKVTHIEIRNTPNLTYIDPDALKELPLLKFLGIFNTGLKMFPPLTKVYSTEIFFILEITDNPYMTSIPRNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTKLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLDVSQTSVTALPSKGLEHLKELRARNTWTLIEGRMDPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
input PDB |
| Selected Chain(s) | A |
| Distance of aggregation | 10 Å |
| FoldX usage | Yes |
| pH calculations | Yes |
| alphaCutter usage | No |
| Dynamic mode | No |
| Automated mutations | No |
| Downloads | Download all the data |
| Simulation log |
[INFO] Logger: Verbosity set to: 2 - [INFO] (00:00:00)
[WARNING] runJob: Working directory already exists (possibly overwriting previous results -ow
to prevent this behavior) (00:00:00)
[INFO] runJob: Starting aggrescan3d job on: input.pdb with A chain(s) selected (00:00:00)
[INFO] runJob: Creating pdb object from: input.pdb (00:00:00)
[INFO] PDB-Info: The input structure is globular. Max score is recommended for pH analysis. (00:00:02)
[INFO] FoldX: Starting FoldX energy minimization (00:00:02)
[INFO] Analysis: Starting Aggrescan4D on folded.pdb (00:07:15)
[INFO] agg3D: Running pKa-ANI on
/STORAGE/DATA/lcbio/aggreskan/363aa902884b75a/tmp/folded.pdb (00:07:15)
[INFO] Main: Simulation completed successfully. (00:09:57)
|
The table below lists A4D score for protein residues. Residues with A4D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan4D score | mutation |
|---|---|---|---|---|
| 1 | M | A | 0.9617 | |
| 2 | G | A | 0.3182 | |
| 3 | C | A | 0.0556 | |
| 4 | S | A | -0.2319 | |
| 5 | S | A | -0.6830 | |
| 6 | P | A | -0.8143 | |
| 7 | P | A | -0.9750 | |
| 8 | C | A | -1.5054 | |
| 9 | E | A | -2.5629 | |
| 10 | C | A | -1.9074 | |
| 11 | H | A | -2.6387 | |
| 12 | Q | A | -2.9264 | |
| 13 | E | A | -3.2561 | |
| 14 | E | A | -3.3719 | |
| 15 | D | A | -2.6783 | |
| 16 | F | A | -1.0283 | |
| 17 | R | A | -2.0095 | |
| 18 | V | A | 0.0000 | |
| 19 | T | A | -1.4670 | |
| 20 | C | A | 0.0000 | |
| 21 | K | A | -2.7310 | |
| 22 | D | A | -3.1479 | |
| 23 | I | A | 0.0000 | |
| 24 | Q | A | -2.3387 | |
| 25 | R | A | -2.0695 | |
| 26 | I | A | -0.5301 | |
| 27 | P | A | -0.5631 | |
| 28 | S | A | -0.4907 | |
| 29 | L | A | -0.3955 | |
| 30 | P | A | -0.5974 | |
| 31 | P | A | -0.8044 | |
| 32 | S | A | -0.5081 | |
| 33 | T | A | 0.0000 | |
| 34 | Q | A | -1.1363 | |
| 35 | T | A | 0.0000 | |
| 36 | L | A | 0.0000 | |
| 37 | K | A | -0.7812 | |
| 38 | L | A | 0.0000 | |
| 39 | I | A | -1.0042 | |
| 40 | E | A | -1.6073 | |
| 41 | T | A | 0.0000 | |
| 42 | C | A | -1.6282 | |
| 43 | L | A | 0.0000 | |
| 44 | R | A | -2.4000 | |
| 45 | T | A | -1.6684 | |
| 46 | I | A | 0.0000 | |
| 47 | P | A | -0.9700 | |
| 48 | S | A | -0.7682 | |
| 49 | H | A | -1.2421 | |
| 50 | A | A | -0.8037 | |
| 51 | F | A | 0.0000 | |
| 52 | S | A | -1.3434 | |
| 53 | N | A | -1.6863 | |
| 54 | L | A | 0.0000 | |
| 55 | P | A | -1.3493 | |
| 56 | N | A | -1.3237 | |
| 57 | I | A | 0.0000 | |
| 58 | S | A | -0.9821 | |
| 59 | R | A | -0.9792 | |
| 60 | I | A | 0.0000 | |
| 61 | Y | A | 0.0000 | |
| 62 | V | A | 0.0000 | |
| 63 | S | A | 0.0000 | |
| 64 | I | A | 0.5820 | |
| 65 | D | A | 0.0000 | |
| 66 | V | A | 1.0181 | |
| 67 | T | A | -0.3919 | |
| 68 | L | A | 0.0000 | |
| 69 | Q | A | -1.4320 | |
| 70 | Q | A | -1.4891 | |
| 71 | L | A | 0.0000 | |
| 72 | E | A | -1.1308 | |
| 73 | S | A | -0.9285 | |
| 74 | H | A | -1.0573 | |
| 75 | S | A | 0.0000 | |
| 76 | F | A | 0.0000 | |
| 77 | Y | A | -1.0282 | |
| 78 | N | A | -1.8406 | |
| 79 | L | A | 0.0000 | |
| 80 | S | A | -1.2184 | |
| 81 | K | A | -1.6278 | |
| 82 | V | A | 0.0000 | |
| 83 | T | A | -0.3743 | |
| 84 | H | A | 0.0000 | |
| 85 | I | A | 0.0000 | |
| 86 | E | A | 0.0000 | |
| 87 | I | A | 0.0000 | |
| 88 | R | A | -0.5586 | |
| 89 | N | A | -0.5622 | |
| 90 | T | A | 0.0000 | |
| 91 | P | A | -0.3505 | |
| 92 | N | A | -0.9856 | |
| 93 | L | A | 0.0000 | |
| 94 | T | A | -0.5890 | |
| 95 | Y | A | -0.3419 | |
| 96 | I | A | 0.0000 | |
| 97 | D | A | -0.6987 | |
| 98 | P | A | -0.6621 | |
| 99 | D | A | -0.9148 | |
| 100 | A | A | 0.0000 | |
| 101 | L | A | 0.0000 | |
| 102 | K | A | -1.0525 | |
| 103 | E | A | -1.1676 | |
| 104 | L | A | 0.0000 | |
| 105 | P | A | -0.3578 | |
| 106 | L | A | 0.2962 | |
| 107 | L | A | 0.0000 | |
| 108 | K | A | -0.0980 | |
| 109 | F | A | 0.0000 | |
| 110 | L | A | 0.0000 | |
| 111 | G | A | 0.0000 | |
| 112 | I | A | 0.0000 | |
| 113 | F | A | 0.0000 | |
| 114 | N | A | -1.1440 | |
| 115 | T | A | 0.0000 | |
| 116 | G | A | 0.0000 | |
| 117 | L | A | 0.0000 | |
| 118 | K | A | -1.0401 | |
| 119 | M | A | -0.0613 | |
| 120 | F | A | 0.0000 | |
| 121 | P | A | 0.0000 | |
| 122 | P | A | -0.2967 | |
| 123 | L | A | 0.0000 | |
| 124 | T | A | -0.5642 | |
| 125 | K | A | -0.6681 | |
| 126 | V | A | 0.0000 | |
| 127 | Y | A | -0.1720 | |
| 128 | S | A | 0.0000 | |
| 129 | T | A | -0.1575 | |
| 130 | E | A | -0.0405 | |
| 131 | I | A | -0.0393 | |
| 132 | F | A | 0.2125 | |
| 133 | F | A | 0.0000 | |
| 134 | I | A | 0.0000 | |
| 135 | L | A | 0.0000 | |
| 136 | E | A | 0.0000 | |
| 137 | I | A | 0.0000 | |
| 138 | T | A | 0.0000 | |
| 139 | D | A | -1.1815 | |
| 140 | N | A | 0.0000 | |
| 141 | P | A | -0.2778 | |
| 142 | Y | A | 0.1490 | |
| 143 | M | A | 0.0000 | |
| 144 | T | A | 0.0574 | |
| 145 | S | A | -0.3137 | |
| 146 | I | A | 0.0000 | |
| 147 | P | A | -1.2041 | |
| 148 | R | A | -2.2622 | |
| 149 | N | A | -1.6538 | |
| 150 | A | A | 0.0000 | |
| 151 | F | A | 0.0000 | |
| 152 | Q | A | -0.9317 | |
| 153 | G | A | -0.5009 | |
| 154 | L | A | 0.0000 | |
| 155 | C | A | 0.0000 | |
| 156 | N | A | -0.7478 | |
| 157 | E | A | -0.5624 | |
| 158 | T | A | -0.2823 | |
| 159 | L | A | 0.0000 | |
| 160 | T | A | 0.0000 | |
| 161 | L | A | 0.0000 | |
| 162 | K | A | 0.0000 | |
| 163 | L | A | 0.0000 | |
| 164 | Y | A | -0.5083 | |
| 165 | N | A | -1.1574 | |
| 166 | N | A | 0.0000 | |
| 167 | G | A | 0.0000 | |
| 168 | F | A | 0.0000 | |
| 169 | T | A | 0.4487 | |
| 170 | S | A | 0.3373 | |
| 171 | V | A | 0.0000 | |
| 172 | Q | A | -1.5557 | |
| 173 | G | A | -1.5828 | |
| 174 | Y | A | -1.1594 | |
| 175 | A | A | 0.0000 | |
| 176 | F | A | 0.0000 | |
| 177 | N | A | -0.8655 | |
| 178 | G | A | 0.0000 | |
| 179 | T | A | 0.0000 | |
| 180 | K | A | -0.4226 | |
| 181 | L | A | 0.0000 | |
| 182 | D | A | 0.0000 | |
| 183 | A | A | 0.0000 | |
| 184 | V | A | 0.0000 | |
| 185 | Y | A | 0.0209 | |
| 186 | L | A | 0.0000 | |
| 187 | N | A | -1.1691 | |
| 188 | K | A | -1.6040 | |
| 189 | N | A | 0.0000 | |
| 190 | K | A | -1.5254 | |
| 191 | Y | A | 0.3965 | |
| 192 | L | A | 0.0000 | |
| 193 | T | A | 0.6684 | |
| 194 | V | A | 1.0518 | |
| 195 | I | A | 0.0000 | |
| 196 | D | A | -1.4703 | |
| 197 | K | A | -2.3665 | |
| 198 | D | A | -1.7967 | |
| 199 | A | A | 0.0000 | |
| 200 | F | A | 0.0000 | |
| 201 | G | A | -0.9490 | |
| 202 | G | A | -0.2722 | |
| 203 | V | A | 0.0145 | |
| 204 | Y | A | 0.4129 | |
| 205 | S | A | -0.2941 | |
| 206 | G | A | -0.8814 | |
| 207 | P | A | 0.0000 | |
| 208 | S | A | -1.0459 | |
| 209 | L | A | 0.0000 | |
| 210 | L | A | 0.0000 | |
| 211 | D | A | 0.0000 | |
| 212 | V | A | 0.0000 | |
| 213 | S | A | 0.0000 | |
| 214 | Q | A | -1.7454 | |
| 215 | T | A | 0.0000 | |
| 216 | S | A | -0.0270 | |
| 217 | V | A | 0.0000 | |
| 218 | T | A | 1.0126 | |
| 219 | A | A | 0.3312 | |
| 220 | L | A | -0.0792 | |
| 221 | P | A | 0.0000 | |
| 222 | S | A | -1.7974 | |
| 223 | K | A | -2.5576 | |
| 224 | G | A | -1.7424 | |
| 225 | L | A | -1.5636 | |
| 226 | E | A | -2.7383 | |
| 227 | H | A | -2.2062 | |
| 228 | L | A | -1.4856 | |
| 229 | K | A | -2.1186 | |
| 230 | E | A | -1.0965 | |
| 231 | L | A | -0.0735 | |
| 232 | R | A | -0.6455 | |
| 233 | A | A | -0.7128 | |
| 234 | R | A | -1.5129 | |
| 235 | N | A | -1.6303 | |
| 236 | T | A | 0.0000 | |
| 237 | W | A | 1.0069 | |
| 238 | T | A | 0.8031 | |
| 239 | L | A | 1.0605 | |
| 240 | I | A | 1.1569 | |
| 241 | E | A | -1.2730 | |
| 242 | G | A | -1.5040 | |
| 243 | R | A | -2.3354 | |
| 244 | M | A | -1.4612 | |
| 245 | D | A | -2.4878 | |
| 246 | P | A | -1.8880 | |
| 247 | K | A | -2.2773 | |
| 248 | S | A | -1.6879 | |
| 249 | C | A | -1.2580 | |
| 250 | D | A | -2.6831 | |
| 251 | K | A | -2.7005 | |
| 252 | T | A | -1.7097 | |
| 253 | H | A | -1.5453 | |
| 254 | T | A | -0.5612 | |
| 255 | C | A | 0.3488 | |
| 256 | P | A | -0.0255 | |
| 257 | P | A | 0.0952 | |
| 258 | C | A | 0.5128 | |
| 259 | P | A | -0.2960 | |
| 260 | A | A | -0.2835 | |
| 261 | P | A | -0.4907 | |
| 262 | E | A | -0.6860 | |
| 263 | L | A | 1.2961 | |
| 264 | L | A | 1.5820 | |
| 265 | G | A | 0.3290 | |
| 266 | G | A | -0.1414 | |
| 267 | P | A | 0.0000 | |
| 268 | S | A | -0.1139 | |
| 269 | V | A | 0.0000 | |
| 270 | F | A | 0.0000 | |
| 271 | L | A | 0.0000 | |
| 272 | F | A | 0.0000 | |
| 273 | P | A | -0.3116 | |
| 274 | P | A | 0.0000 | |
| 275 | K | A | -1.0417 | |
| 276 | P | A | -0.9060 | |
| 277 | K | A | -1.2160 | |
| 278 | D | A | 0.0000 | |
| 279 | T | A | 0.0000 | |
| 280 | L | A | 0.0000 | |
| 281 | M | A | 0.1540 | |
| 282 | I | A | 1.3342 | |
| 283 | S | A | -0.0140 | |
| 284 | R | A | -1.3816 | |
| 285 | T | A | -0.6942 | |
| 286 | P | A | 0.0000 | |
| 287 | E | A | -0.9049 | |
| 288 | V | A | 0.0000 | |
| 289 | T | A | 0.0000 | |
| 290 | C | A | 0.0000 | |
| 291 | V | A | 0.0000 | |
| 292 | V | A | 0.0000 | |
| 293 | V | A | 0.0000 | |
| 294 | D | A | -0.9341 | |
| 295 | V | A | 0.0000 | |
| 296 | S | A | -2.1641 | |
| 297 | H | A | -2.5792 | |
| 298 | E | A | -2.9674 | |
| 299 | D | A | -2.5927 | |
| 300 | P | A | -2.5514 | |
| 301 | E | A | -3.0905 | |
| 302 | V | A | -1.9277 | |
| 303 | K | A | -2.2457 | |
| 304 | F | A | -1.1792 | |
| 305 | N | A | -1.1699 | |
| 306 | W | A | 0.0000 | |
| 307 | Y | A | -0.6909 | |
| 308 | V | A | -0.9156 | |
| 309 | D | A | -2.1725 | |
| 310 | G | A | -0.9295 | |
| 311 | V | A | 0.5243 | |
| 312 | E | A | -0.7644 | |
| 313 | V | A | -0.5822 | |
| 314 | H | A | -1.9007 | |
| 315 | N | A | -2.1815 | |
| 316 | A | A | -1.8553 | |
| 317 | K | A | -2.3971 | |
| 318 | T | A | -1.8477 | |
| 319 | K | A | -2.0454 | |
| 320 | P | A | -2.1216 | |
| 321 | R | A | -3.1009 | |
| 322 | E | A | -3.2946 | |
| 323 | E | A | -3.2153 | |
| 324 | Q | A | -1.6819 | |
| 325 | Y | A | -0.6956 | |
| 326 | N | A | -0.8316 | |
| 327 | S | A | -1.0509 | |
| 328 | T | A | -1.7740 | |
| 329 | Y | A | 0.0000 | |
| 330 | R | A | 0.0000 | |
| 331 | V | A | 0.0000 | |
| 332 | V | A | 0.0000 | |
| 333 | S | A | 0.0000 | |
| 334 | V | A | 0.0000 | |
| 335 | L | A | 0.0000 | |
| 336 | T | A | -0.6306 | |
| 337 | V | A | 0.0000 | |
| 338 | L | A | 0.6193 | |
| 339 | H | A | -0.1981 | |
| 340 | Q | A | -1.1797 | |
| 341 | D | A | -1.4016 | |
| 342 | W | A | 0.0000 | |
| 343 | L | A | -0.9641 | |
| 344 | N | A | -2.0602 | |
| 345 | G | A | -2.0529 | |
| 346 | K | A | -2.1519 | |
| 347 | E | A | -2.1668 | |
| 348 | Y | A | 0.0000 | |
| 349 | K | A | -1.5233 | |
| 350 | C | A | 0.0000 | |
| 351 | K | A | -1.3648 | |
| 352 | V | A | 0.0000 | |
| 353 | S | A | -1.4060 | |
| 354 | N | A | 0.0000 | |
| 355 | K | A | -2.5553 | |
| 356 | A | A | -1.3942 | |
| 357 | L | A | -0.5918 | |
| 358 | P | A | -0.5962 | |
| 359 | A | A | -0.5936 | |
| 360 | P | A | -0.7590 | |
| 361 | I | A | -0.4109 | |
| 362 | E | A | -1.6805 | |
| 363 | K | A | -1.0602 | |
| 364 | T | A | -0.8677 | |
| 365 | I | A | -0.3425 | |
| 366 | S | A | -1.2514 | |
| 367 | K | A | -1.2603 | |
| 368 | A | A | -1.1770 | |
| 369 | K | A | -2.3485 | |
| 370 | G | A | -1.9522 | |
| 371 | Q | A | -2.0850 | |
| 372 | P | A | -1.6663 | |
| 373 | R | A | -2.0449 | |
| 374 | E | A | -2.6891 | |
| 375 | P | A | 0.0000 | |
| 376 | Q | A | -1.3241 | |
| 377 | V | A | -0.4757 | |
| 378 | Y | A | 0.5471 | |
| 379 | T | A | 0.3000 | |
| 380 | L | A | 0.5006 | |
| 381 | P | A | -0.2085 | |
| 382 | P | A | -0.9774 | |
| 383 | S | A | -1.7416 | |
| 384 | R | A | -2.8903 | |
| 385 | D | A | -3.1312 | |
| 386 | E | A | -2.6698 | |
| 387 | L | A | -2.3404 | |
| 388 | T | A | -2.1601 | |
| 389 | K | A | -3.1030 | |
| 390 | N | A | -2.8985 | |
| 391 | Q | A | -2.5967 | |
| 392 | V | A | 0.0000 | |
| 393 | S | A | -0.7380 | |
| 394 | L | A | 0.0000 | |
| 395 | T | A | -0.1814 | |
| 396 | C | A | 0.0000 | |
| 397 | L | A | 0.4644 | |
| 398 | V | A | 0.0000 | |
| 399 | K | A | -0.8543 | |
| 400 | G | A | -1.4336 | |
| 401 | F | A | 0.0000 | |
| 402 | Y | A | -1.0880 | |
| 403 | P | A | 0.0000 | |
| 404 | S | A | -0.0571 | |
| 405 | D | A | -0.9817 | |
| 406 | I | A | -0.4902 | |
| 407 | A | A | -0.5158 | |
| 408 | V | A | 0.0000 | |
| 409 | E | A | -1.4056 | |
| 410 | W | A | 0.0000 | |
| 411 | E | A | -1.8408 | |
| 412 | S | A | 0.0000 | |
| 413 | N | A | -1.7997 | |
| 414 | G | A | -1.7435 | |
| 415 | Q | A | -2.2698 | |
| 416 | P | A | -1.9583 | |
| 417 | E | A | 0.0000 | |
| 418 | N | A | -2.3857 | |
| 419 | N | A | -2.2171 | |
| 420 | Y | A | -2.0779 | |
| 421 | K | A | -2.3547 | |
| 422 | T | A | -1.0099 | |
| 423 | T | A | -0.2904 | |
| 424 | P | A | -0.0262 | |
| 425 | P | A | 0.0000 | |
| 426 | V | A | 1.0501 | |
| 427 | L | A | 0.8824 | |
| 428 | D | A | -0.8142 | |
| 429 | S | A | -1.1872 | |
| 430 | D | A | -2.0629 | |
| 431 | G | A | -0.9733 | |
| 432 | S | A | 0.0000 | |
| 433 | F | A | 0.0000 | |
| 434 | F | A | 0.4196 | |
| 435 | L | A | 0.0000 | |
| 436 | Y | A | 0.3373 | |
| 437 | S | A | 0.0000 | |
| 438 | K | A | -1.7012 | |
| 439 | L | A | 0.0000 | |
| 440 | T | A | -1.4548 | |
| 441 | V | A | 0.0000 | |
| 442 | D | A | -2.3836 | |
| 443 | K | A | -2.6405 | |
| 444 | S | A | -2.0736 | |
| 445 | R | A | -1.8122 | |
| 446 | W | A | 0.0000 | |
| 447 | Q | A | -2.2355 | |
| 448 | Q | A | -2.0151 | |
| 449 | G | A | -1.0312 | |
| 450 | N | A | -0.6096 | |
| 451 | V | A | 0.4145 | |
| 452 | F | A | 0.0000 | |
| 453 | S | A | -1.0864 | |
| 454 | C | A | 0.0000 | |
| 455 | S | A | 0.0000 | |
| 456 | V | A | 0.0000 | |
| 457 | M | A | -0.5934 | |
| 458 | H | A | 0.0000 | |
| 459 | E | A | -1.0734 | |
| 460 | A | A | -1.4193 | |
| 461 | L | A | -1.4192 | |
| 462 | H | A | -1.7483 | |
| 463 | N | A | -1.6578 | |
| 464 | H | A | -1.1868 | |
| 465 | Y | A | -0.4954 | |
| 466 | T | A | -0.9845 | |
| 467 | Q | A | -1.5845 | |
| 468 | K | A | -1.2973 | |
| 469 | S | A | -0.4846 | |
| 470 | L | A | 0.3251 | |
| 471 | S | A | 0.0615 | |
| 472 | L | A | -0.2994 | |
| 473 | S | A | -0.6984 | |
| 474 | P | A | -0.9299 | |
| 475 | G | A | -1.4174 | |
| 476 | K | A | -2.0233 |
Calculations for various pH values
This page contains details and comparisons for all models calculated at different pH points.
Please find suggestions on interpreting the results below. More details can be found in the Tutorial.
The input structure is globular. Max score is recommended for pH analysis.
pH |
Average A4D Score |
Max A4D Score |
|||
| 4.0 | -0.5952 | 4.5618 | View | CSV | PDB |
| 4.5 | -0.6495 | 4.5618 | View | CSV | PDB |
| 5.0 | -0.7149 | 4.5618 | View | CSV | PDB |
| 5.5 | -0.7773 | 4.5618 | View | CSV | PDB |
| 6.0 | -0.8216 | 4.5618 | View | CSV | PDB |
| 6.5 | -0.8363 | 4.5618 | View | CSV | PDB |
| 7.0 | -0.8224 | 4.5618 | View | CSV | PDB |
| 7.5 | -0.7908 | 4.5618 | View | CSV | PDB |
| 8.0 | -0.7501 | 4.5618 | View | CSV | PDB |
| 8.5 | -0.7032 | 4.5618 | View | CSV | PDB |
| 9.0 | -0.6502 | 4.5618 | View | CSV | PDB |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine