Aggrescan4D: 445bd48d49b53be

Project name: 445bd48d49b53be

Status: done

Started: 2026-05-20 08:01:34

Chain sequence(s)	A: EVQLVESGGGLVKPGGSLKLSCAASGYTFTSYVMHWVRQAPGKGLEWIGYINPYNDGTKYNEKFQGRVTISSDKSISTAYMELSSLRSEDTAMYYCARGTYYYGTRVFDYWGQGTLVTVSSGGGGSGGGGSGGGGSDIVMTQSPATLSLSPGERATLSCRSSKSLQNVNGNTYLYWFQQKPGQSPQLLIYRMSNLNSGVPDRFSGSGSGTEFTLTISSLEPEDFAVYYCMQHLEYPITFGAGTKLEIKSGGGGSEVQLVESGGGLVQPGRSLRLSCAASGFTFDDYAMHWVRQAPGKGLEWVSGISWNSGSIGYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTALYYCAKDSRGYGDYRLGGAYWGQGTLVTVSSGGGGSGGGGSGGGGSEIVMTQSPATLSVSPGERATLSCRASQSVSSNLAWYQQKPGQAPRLLIYGASTRATGIPARFSGSGSGTEFTLTISSLQSEDFAVYYCQQYNNWPWTFGQGTKVEIKGGGGDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLYITREPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALAAPIEKTISKAKGQPREPQVYTLPPCREEMTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK B: DVQLVESGGGLVQAGGSLRLSCRASGHTFSDTAVGWFRRVPGKEREFVATIAGSGSVRYSESVKGRFTISGDNAKNMVYLQMNRLKPEDTGVYYCAAAVGISYDYWGQGTQVTVSSEPKSSDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLYITREPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALAAPIEKTISKAKGQPREPQVCTLPPSREEMTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK input PDB
Selected Chain(s)	A,B
Distance of aggregation	10 Å
FoldX usage	Yes
pH calculations	No
alphaCutter usage	No
Dynamic mode	No
Automated mutations	Yes
Downloads	Download all the data

Simulation log

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[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:12)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:12)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:12)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:12)
[INFO]       FoldX:    Starting FoldX energy minimization                                          (00:00:13)
[INFO]       Analysis: Starting Aggrescan4D on folded.pdb                                          (00:23:33)
[INFO]       AutoMut:  Residue number 101 from chain B and a score of 1.808 (isoleucine) selected  
                       for automated mutation                                                      (00:23:52)
[INFO]       AutoMut:  Residue number 536 from chain A and a score of 1.370 (isoleucine) selected  
                       for automated mutation                                                      (00:23:52)
[INFO]       AutoMut:  Residue number 76 from chain A and a score of 1.353 (isoleucine) selected   
                       for automated mutation                                                      (00:23:52)
[INFO]       AutoMut:  Residue number 11 from chain A and a score of 1.235 (leucine) selected for  
                       automated mutation                                                          (00:23:52)
[INFO]       AutoMut:  Residue number 154 from chain B and a score of 1.186 (isoleucine) selected  
                       for automated mutation                                                      (00:23:52)
[INFO]       AutoMut:  Residue number 116 from chain A and a score of 1.095 (leucine) selected for 
                       automated mutation                                                          (00:23:52)
[INFO]       AutoMut:  Mutating residue number 101 from chain B (isoleucine) into glutamic acid    (00:23:52)
[INFO]       AutoMut:  Mutating residue number 536 from chain A (isoleucine) into glutamic acid    (00:23:52)
[INFO]       AutoMut:  Mutating residue number 101 from chain B (isoleucine) into aspartic acid    (00:23:52)
[INFO]       AutoMut:  Mutating residue number 101 from chain B (isoleucine) into arginine         (00:24:40)
[INFO]       AutoMut:  Mutating residue number 101 from chain B (isoleucine) into lysine           (00:24:42)
[INFO]       AutoMut:  Mutating residue number 536 from chain A (isoleucine) into lysine           (00:24:55)
[INFO]       AutoMut:  Mutating residue number 536 from chain A (isoleucine) into aspartic acid    (00:25:29)
[INFO]       AutoMut:  Mutating residue number 76 from chain A (isoleucine) into glutamic acid     (00:25:47)
[INFO]       AutoMut:  Mutating residue number 76 from chain A (isoleucine) into aspartic acid     (00:25:57)
[INFO]       AutoMut:  Mutating residue number 536 from chain A (isoleucine) into arginine         (00:26:17)
[INFO]       AutoMut:  Mutating residue number 76 from chain A (isoleucine) into lysine            (00:26:39)
[INFO]       AutoMut:  Mutating residue number 76 from chain A (isoleucine) into arginine          (00:26:41)
[INFO]       AutoMut:  Mutating residue number 11 from chain A (leucine) into glutamic acid        (00:27:11)
[INFO]       AutoMut:  Mutating residue number 11 from chain A (leucine) into aspartic acid        (00:27:41)
[INFO]       AutoMut:  Mutating residue number 154 from chain B (isoleucine) into glutamic acid    (00:27:43)
[INFO]       AutoMut:  Mutating residue number 11 from chain A (leucine) into lysine               (00:27:55)
[INFO]       AutoMut:  Mutating residue number 11 from chain A (leucine) into arginine             (00:28:23)
[INFO]       AutoMut:  Mutating residue number 154 from chain B (isoleucine) into lysine           (00:28:40)
[INFO]       AutoMut:  Mutating residue number 154 from chain B (isoleucine) into aspartic acid    (00:28:49)
[INFO]       AutoMut:  Mutating residue number 116 from chain A (leucine) into glutamic acid       (00:29:12)
[INFO]       AutoMut:  Mutating residue number 154 from chain B (isoleucine) into arginine         (00:29:37)
[INFO]       AutoMut:  Mutating residue number 116 from chain A (leucine) into lysine              (00:30:05)
[INFO]       AutoMut:  Mutating residue number 116 from chain A (leucine) into aspartic acid       (00:30:12)
[INFO]       AutoMut:  Mutating residue number 116 from chain A (leucine) into arginine            (00:30:57)
[INFO]       AutoMut:  Effect of mutation residue number 101 from chain B (isoleucine) into        
                       glutamic acid: Energy difference: 0.0420 kcal/mol, Difference in average    
                       score from the base case: -0.0108                                           (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 101 from chain B (isoleucine) into        
                       lysine: Energy difference: 0.4433 kcal/mol, Difference in average score     
                       from the base case: -0.0111                                                 (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 101 from chain B (isoleucine) into        
                       aspartic acid: Energy difference: 0.6124 kcal/mol, Difference in average    
                       score from the base case: -0.0103                                           (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 101 from chain B (isoleucine) into        
                       arginine: Energy difference: 0.4937 kcal/mol, Difference in average score   
                       from the base case: -0.0097                                                 (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 536 from chain A (isoleucine) into        
                       glutamic acid: Energy difference: -0.3878 kcal/mol, Difference in average   
                       score from the base case: -0.0108                                           (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 536 from chain A (isoleucine) into        
                       lysine: Energy difference: -0.1376 kcal/mol, Difference in average score    
                       from the base case: -0.0092                                                 (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 536 from chain A (isoleucine) into        
                       aspartic acid: Energy difference: 0.1775 kcal/mol, Difference in average    
                       score from the base case: -0.0109                                           (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 536 from chain A (isoleucine) into        
                       arginine: Energy difference: -0.3727 kcal/mol, Difference in average score  
                       from the base case: -0.0096                                                 (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 76 from chain A (isoleucine) into         
                       glutamic acid: Energy difference: 0.9971 kcal/mol, Difference in average    
                       score from the base case: -0.0106                                           (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 76 from chain A (isoleucine) into lysine: 
                       Energy difference: -0.6080 kcal/mol, Difference in average score from the   
                       base case: -0.0102                                                          (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 76 from chain A (isoleucine) into         
                       aspartic acid: Energy difference: 0.5784 kcal/mol, Difference in average    
                       score from the base case: -0.0105                                           (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 76 from chain A (isoleucine) into         
                       arginine: Energy difference: -0.6768 kcal/mol, Difference in average score  
                       from the base case: -0.0098                                                 (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain A (leucine) into glutamic   
                       acid: Energy difference: 0.5965 kcal/mol, Difference in average score from  
                       the base case: -0.0111                                                      (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain A (leucine) into lysine:    
                       Energy difference: 0.2619 kcal/mol, Difference in average score from the    
                       base case: -0.0107                                                          (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain A (leucine) into aspartic   
                       acid: Energy difference: 0.2786 kcal/mol, Difference in average score from  
                       the base case: -0.0114                                                      (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain A (leucine) into arginine:  
                       Energy difference: 0.4033 kcal/mol, Difference in average score from the    
                       base case: -0.0108                                                          (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 154 from chain B (isoleucine) into        
                       glutamic acid: Energy difference: -0.5567 kcal/mol, Difference in average   
                       score from the base case: -0.0126                                           (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 154 from chain B (isoleucine) into        
                       lysine: Energy difference: -0.2677 kcal/mol, Difference in average score    
                       from the base case: -0.0118                                                 (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 154 from chain B (isoleucine) into        
                       aspartic acid: Energy difference: 0.0230 kcal/mol, Difference in average    
                       score from the base case: -0.0125                                           (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 154 from chain B (isoleucine) into        
                       arginine: Energy difference: -0.6515 kcal/mol, Difference in average score  
                       from the base case: -0.0126                                                 (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 116 from chain A (leucine) into glutamic  
                       acid: Energy difference: 0.7894 kcal/mol, Difference in average score from  
                       the base case: -0.0094                                                      (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 116 from chain A (leucine) into lysine:   
                       Energy difference: -0.0303 kcal/mol, Difference in average score from the   
                       base case: -0.0098                                                          (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 116 from chain A (leucine) into aspartic  
                       acid: Energy difference: 1.1461 kcal/mol, Difference in average score from  
                       the base case: -0.0089                                                      (00:31:46)
[INFO]       AutoMut:  Effect of mutation residue number 116 from chain A (leucine) into arginine: 
                       Energy difference: -0.0100 kcal/mol, Difference in average score from the   
                       base case: -0.0102                                                          (00:31:46)
[INFO]       Main:     Simulation completed successfully.                                          (00:32:00)

Note Your prediction results will be deleted after 360 days.

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Show buried residues

Minimal score value: -3.1499
Maximal score value: 1.8075
Average score: -0.7154
Total score value: -771.1533

Download the table (csv)

The table below lists A4D score for protein residues. Residues with A4D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan4D score

1	E	A	-1.9717
2	V	A	0.0000
3	Q	A	-1.5169
4	L	A	0.0000
5	V	A	0.6402
6	E	A	0.0000
7	S	A	-0.2954
8	G	A	-0.6650
9	G	A	-0.0175
10	G	A	0.7269
11	L	A	1.2349
12	V	A	-0.3317
13	K	A	-1.9360
14	P	A	-1.9624
15	G	A	-1.4783
16	G	A	-1.1611
17	S	A	-1.2496
18	L	A	-1.0694
19	K	A	-2.1220
20	L	A	0.0000
21	S	A	-0.4415
22	C	A	0.0000
23	A	A	0.5016
24	A	A	0.0000
25	S	A	-0.5872
26	G	A	-0.8660
27	Y	A	-0.1781
28	T	A	-0.1145
29	F	A	0.0000
30	T	A	-0.2540
31	S	A	0.0000
32	Y	A	0.0000
33	V	A	0.0000
34	M	A	0.0000
35	H	A	0.0000
36	W	A	0.0000
37	V	A	0.0000
38	R	A	0.0000
39	Q	A	-0.6543
40	A	A	-1.0640
41	P	A	-0.9016
42	G	A	-1.4536
43	K	A	-2.1359
44	G	A	-1.1317
45	L	A	0.0000
46	E	A	-0.5955
47	W	A	0.0000
48	I	A	0.0000
49	G	A	0.0000
50	Y	A	0.0000
51	I	A	0.0000
52	N	A	0.0000
53	P	A	0.0000
54	Y	A	-0.4135
55	N	A	-1.3419
56	D	A	-2.0028
57	G	A	-1.1308
58	T	A	-0.7961
59	K	A	-0.5877
60	Y	A	-0.9664
61	N	A	-1.5828
62	E	A	-3.1056
63	K	A	-3.0950
64	F	A	0.0000
65	Q	A	-2.7224
66	G	A	-1.6993
67	R	A	-1.5748
68	V	A	0.0000
69	T	A	-0.9007
70	I	A	0.0000
71	S	A	-0.7811
72	S	A	-0.7177
73	D	A	-0.8440
74	K	A	-0.8846
75	S	A	0.0517
76	I	A	1.3525
77	S	A	0.2574
78	T	A	0.0000
79	A	A	0.0000
80	Y	A	-0.6411
81	M	A	0.0000
82	E	A	-1.6294
83	L	A	0.0000
84	S	A	-1.1358
85	S	A	-1.1807
86	L	A	0.0000
87	R	A	-2.6576
88	S	A	-2.0816
89	E	A	-2.2144
90	D	A	0.0000
91	T	A	-0.5330
92	A	A	0.0000
93	M	A	0.4469
94	Y	A	0.0000
95	Y	A	0.0000
96	C	A	0.0000
97	A	A	0.0000
98	R	A	0.0000
99	G	A	0.0000
100	T	A	0.0000
101	Y	A	0.0000
102	Y	A	0.0000
103	Y	A	-0.0586
104	G	A	0.0000
105	T	A	-0.4551
106	R	A	-0.6870
107	V	A	0.0000
108	F	A	0.0000
109	D	A	0.0000
110	Y	A	-0.2294
111	W	A	0.0000
112	G	A	0.0000
113	Q	A	-1.1745
114	G	A	0.0000
115	T	A	0.3188
116	L	A	1.0952
117	V	A	0.0000
118	T	A	0.1092
119	V	A	0.0000
120	S	A	-1.0674
121	S	A	-1.6082
122	G	A	-1.3422
123	G	A	-1.1118
124	G	A	-1.2044
125	G	A	-1.1420
126	S	A	-0.9968
127	G	A	-1.1689
128	G	A	-1.1924
129	G	A	-1.1834
130	G	A	-1.1855
131	S	A	-1.0137
132	G	A	-1.3706
133	G	A	-1.1724
134	G	A	-1.2702
135	G	A	-1.4933
136	S	A	-1.1697
137	D	A	-1.2903
138	I	A	0.0000
139	V	A	0.7665
140	M	A	0.0000
141	T	A	-0.4756
142	Q	A	0.0000
143	S	A	-0.5522
144	P	A	-0.2819
145	A	A	-0.1993
146	T	A	-0.4783
147	L	A	-0.0838
148	S	A	-0.1203
149	L	A	-0.3036
150	S	A	0.0000
151	P	A	-0.8041
152	G	A	0.0000
153	E	A	-1.7689
154	R	A	-2.3169
155	A	A	0.0000
156	T	A	-0.5844
157	L	A	0.0000
158	S	A	-0.9882
159	C	A	0.0000
160	R	A	-2.6373
161	S	A	0.0000
162	S	A	-1.2521
163	K	A	-2.2596
164	S	A	-1.8248
165	L	A	0.0000
166	Q	A	-0.9114
167	N	A	0.0777
168	V	A	0.9782
169	N	A	0.0000
170	G	A	-0.5419
171	N	A	-0.4605
172	T	A	0.0000
173	Y	A	0.0000
174	L	A	0.0000
175	Y	A	0.0000
176	W	A	0.0000
177	F	A	0.0000
178	Q	A	-0.6925
179	Q	A	0.0000
180	K	A	-1.4480
181	P	A	-0.9838
182	G	A	-1.3703
183	Q	A	-1.9401
184	S	A	-1.3246
185	P	A	0.0000
186	Q	A	-0.9882
187	L	A	0.0000
188	L	A	0.0000
189	I	A	0.0000
190	Y	A	-0.4308
191	R	A	-0.8386
192	M	A	-0.5754
193	S	A	-0.6474
194	N	A	-0.8810
195	L	A	-0.4288
196	N	A	0.0000
197	S	A	-0.4679
198	G	A	-0.6251
199	V	A	-0.5345
200	P	A	-1.0029
201	D	A	-1.8975
202	R	A	-1.4073
203	F	A	0.0000
204	S	A	-0.6985
205	G	A	0.0000
206	S	A	-0.8624
207	G	A	-1.2859
208	S	A	-1.2816
209	G	A	-1.5890
210	T	A	-2.2685
211	E	A	-2.9250
212	F	A	0.0000
213	T	A	-0.9087
214	L	A	0.0000
215	T	A	-0.5891
216	I	A	0.0000
217	S	A	-1.4058
218	S	A	-1.3738
219	L	A	0.0000
220	E	A	-1.5991
221	P	A	-1.2472
222	E	A	-2.1988
223	D	A	0.0000
224	F	A	0.0000
225	A	A	0.0000
226	V	A	-0.4081
227	Y	A	0.0000
228	Y	A	0.0000
229	C	A	0.0000
230	M	A	0.0000
231	Q	A	0.0000
232	H	A	0.0000
233	L	A	-0.5382
234	E	A	-0.8156
235	Y	A	0.1524
236	P	A	-0.5173
237	I	A	0.0000
238	T	A	0.2150
239	F	A	0.3953
240	G	A	0.0000
241	A	A	-0.1905
242	G	A	0.0000
243	T	A	0.0000
244	K	A	-0.8042
245	L	A	0.0000
246	E	A	-0.3768
247	I	A	1.0105
248	K	A	-0.0866
249	S	A	-0.3653
250	G	A	-0.8029
251	G	A	-1.0224
252	G	A	-1.0559
253	G	A	-1.0546
254	S	A	-1.2546
255	E	A	-1.6557
256	V	A	-0.5882
257	Q	A	-0.5167
258	L	A	0.0000
259	V	A	0.3123
260	E	A	0.0000
261	S	A	-0.6454
262	G	A	-0.9742
263	G	A	-0.7192
264	G	A	-0.4357
265	L	A	0.1498
266	V	A	-0.7671
267	Q	A	-1.6943
268	P	A	-2.0716
269	G	A	-2.1013
270	R	A	-2.6810
271	S	A	-1.9364
272	L	A	-1.5800
273	R	A	-2.1618
274	L	A	0.0000
275	S	A	-0.5999
276	C	A	0.0000
277	A	A	-0.2803
278	A	A	0.0000
279	S	A	-0.5129
280	G	A	0.0000
281	F	A	-0.4746
282	T	A	-0.5294
283	F	A	0.0000
284	D	A	-1.5195
285	D	A	-0.8580
286	Y	A	-0.3661
287	A	A	0.0000
288	M	A	0.0000
289	H	A	0.0000
290	W	A	0.0000
291	V	A	0.0000
292	R	A	0.0000
293	Q	A	-0.7714
294	A	A	-1.3994
295	P	A	0.0000
296	G	A	-1.7038
297	K	A	-2.5248
298	G	A	-1.5823
299	L	A	0.0000
300	E	A	-1.0592
301	W	A	0.0000
302	V	A	0.0000
303	S	A	0.0000
304	G	A	0.0000
305	I	A	0.0000
306	S	A	0.0000
307	W	A	-0.2851
308	N	A	-1.2632
309	S	A	-0.7698
310	G	A	-0.5423
311	S	A	0.1445
312	I	A	1.0274
313	G	A	0.2392
314	Y	A	-0.6169
315	A	A	0.0000
316	D	A	-2.7039
317	S	A	-1.7978
318	V	A	0.0000
319	K	A	-2.5324
320	G	A	-1.6365
321	R	A	0.0000
322	F	A	0.0000
323	T	A	-0.6589
324	I	A	0.0000
325	S	A	-0.3724
326	R	A	-1.1980
327	D	A	-1.8646
328	N	A	-2.0289
329	A	A	-1.5080
330	K	A	-2.2780
331	N	A	-1.8658
332	S	A	-1.2403
333	L	A	0.0000
334	Y	A	-0.6408
335	L	A	0.0000
336	Q	A	-1.2306
337	M	A	0.0000
338	N	A	-1.8733
339	S	A	-1.7141
340	L	A	0.0000
341	R	A	-2.3499
342	A	A	-2.0027
343	E	A	-2.4521
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276	S	B	0.0000
277	D	B	-1.0577
278	I	B	-0.5059
279	A	B	0.0000
280	V	B	0.0000
281	E	B	-0.2012
282	W	B	0.0000
283	E	B	0.0000
284	S	B	0.0000
285	N	B	-0.7118
286	G	B	0.0000
287	Q	B	-0.1383
288	P	B	0.0000
289	E	B	0.0000
290	N	B	0.0000
291	N	B	0.0758
292	Y	B	-0.2215
293	K	B	-0.3675
294	T	B	-0.1637
295	T	B	0.0000
296	P	B	-0.3947
297	P	B	-0.0635
298	V	B	0.0000
299	L	B	-0.0289
300	D	B	-0.9065
301	S	B	-1.2913
302	D	B	-2.0229
303	G	B	-1.1099
304	S	B	0.0000
305	F	B	0.0000
306	F	B	0.0000
307	L	B	0.0000
308	V	B	0.0000
309	S	B	0.0000
310	K	B	0.0000
311	L	B	0.0000
312	T	B	-0.2529
313	V	B	0.0000
314	D	B	-2.2913
315	K	B	-2.3913
316	S	B	-2.1464
317	R	B	-1.9680
318	W	B	0.0000
319	Q	B	-2.3213
320	Q	B	-2.1132
321	G	B	-1.2100
322	N	B	-0.7822
323	V	B	0.2537
324	F	B	0.0000
325	S	B	0.0000
326	C	B	0.0000
327	S	B	0.0000
328	V	B	0.0000
329	M	B	0.0000
330	H	B	0.0000
331	E	B	-1.1488
332	A	B	-1.6038
333	L	B	0.0000
334	H	B	-1.9414
335	N	B	-1.8668
336	H	B	-1.4294
337	Y	B	-0.7151
338	T	B	-0.5165
339	Q	B	0.0000
340	K	B	-0.5398
341	S	B	-0.3644
342	L	B	0.0000
343	S	B	-0.1655
344	L	B	-0.2808
345	S	B	-0.6493
346	P	B	-1.0564
347	G	B	-1.4034
348	K	B	-2.0273

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Automated mutations analysis - charged mutations

In the automated mutations mode, the server selects aggregation prone resides and each selected residue is mutated to glutamic acid, lysine, aspartic acid and arginine. The table below shows 2 best scored mutants for each mutated residue. Protein variants are ordered according to the mutation effect they had on protein stability (energetic effect) together with the difference in the average per-residue aggregation score between the wild type and the mutant (in the table green values indicate a positive change, grey are neutral, and orange/red mean destabilizing or more aggregation prone mutants).
Summary for all the mutants can be found in this CSV file .

Mutant	Energetic effect	Score comparison
IR154B	-0.6515	-0.0126	View	CSV	PDB
IE154B	-0.5567	-0.0126	View	CSV	PDB
IR76A	-0.6768	-0.0098	View	CSV	PDB
IK76A	-0.608	-0.0102	View	CSV	PDB
IE536A	-0.3878	-0.0108	View	CSV	PDB
IR536A	-0.3727	-0.0096	View	CSV	PDB
LR116A	-0.01	-0.0102	View	CSV	PDB
LK116A	-0.0303	-0.0098	View	CSV	PDB
IE101B	0.042	-0.0108	View	CSV	PDB
LD11A	0.2786	-0.0114	View	CSV	PDB
LK11A	0.2619	-0.0107	View	CSV	PDB
IK101B	0.4433	-0.0111	View	CSV	PDB

Project name: 445bd48d49b53be

Status: done

Started: 2026-05-20 08:01:34

Automated mutations analysis - charged mutations

Mutant

Energetic effect

Score comparison