Aggrescan4D: 7cc8956f57d549b

Project name: 7cc8956f57d549b

Status: done

Started: 2026-05-20 08:57:31

Chain sequence(s)	A: EVQLVESGGGLVKPGGSLKLSCAASGYTFTSYVMHWVRQAPGKGLEWIGYINPYNDGTKYNEKFQGRVTISSDKSISTAYMELSSLRSEDTAMYYCARGTYYYGTRVFDYWGQGTLVTVSSGGGGSGGGGSGGGGSDIVMTQSPATLSLSPGERATLSCRSSKSLQNVNGNTYLYWFQQKPGQSPQLLIYRMSNLNSGVPDRFSGSGSGTEFTLTISSLEPEDFAVYYCMQHLEYPITFGAGTKLEIKSGGGGSEVQLVESGGGLVQPGRSLRLSCAASGFTFDDYAMHWVRQAPGKGLEWVSGISWNSGSIGYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTALYYCAKDSRGYGDYRLGGAYWGQGTLVTVSSGGGGSGGGGSGGGGSEIVMTQSPATLSVSPGERATLSCRASQSVSSNLAWYQQKPGQAPRLLIYGASTRATGIPARFSGSGSGTEFTLTISSLQSEDFAVYYCQQYNNWPWTFGQGTKVEIKGGGGDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLYITREPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALAAPIEKTISKAKGQPREPQVYTLPPCREEMTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK B: EVQLLESGGGLVQPGGSLRLSCAASGFTFDDYAIGWFRQAPGKEREGVSCIRVSDGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAAGSLYTCVQSIVWPARPYYDMDYWGQGTLVTVSSEPKSSDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLYITREPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALAAPIEKTISKAKGQPREPQVCTLPPSREEMTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK input PDB
Selected Chain(s)	A,B
Distance of aggregation	10 Å
FoldX usage	Yes
pH calculations	No
alphaCutter usage	No
Dynamic mode	No
Automated mutations	Yes
Downloads	Download all the data

Simulation log

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[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:04)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:04)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:04)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:04)
[INFO]       FoldX:    Starting FoldX energy minimization                                          (00:00:04)
[INFO]       Analysis: Starting Aggrescan4D on folded.pdb                                          (00:22:12)
[INFO]       AutoMut:  Residue number 169 from chain B and a score of 1.712 (isoleucine) selected  
                       for automated mutation                                                      (00:22:28)
[INFO]       AutoMut:  Residue number 372 from chain A and a score of 1.565 (leucine) selected for 
                       automated mutation                                                          (00:22:28)
[INFO]       AutoMut:  Residue number 265 from chain A and a score of 1.380 (leucine) selected for 
                       automated mutation                                                          (00:22:29)
[INFO]       AutoMut:  Residue number 76 from chain A and a score of 1.341 (isoleucine) selected   
                       for automated mutation                                                      (00:22:29)
[INFO]       AutoMut:  Residue number 536 from chain A and a score of 1.330 (isoleucine) selected  
                       for automated mutation                                                      (00:22:29)
[INFO]       AutoMut:  Residue number 11 from chain A and a score of 1.281 (leucine) selected for  
                       automated mutation                                                          (00:22:29)
[INFO]       AutoMut:  Mutating residue number 169 from chain B (isoleucine) into glutamic acid    (00:22:29)
[INFO]       AutoMut:  Mutating residue number 169 from chain B (isoleucine) into aspartic acid    (00:22:29)
[INFO]       AutoMut:  Mutating residue number 372 from chain A (leucine) into glutamic acid       (00:22:29)
[INFO]       AutoMut:  Mutating residue number 372 from chain A (leucine) into lysine              (00:23:13)
[INFO]       AutoMut:  Mutating residue number 169 from chain B (isoleucine) into arginine         (00:23:16)
[INFO]       AutoMut:  Mutating residue number 169 from chain B (isoleucine) into lysine           (00:23:26)
[INFO]       AutoMut:  Mutating residue number 372 from chain A (leucine) into aspartic acid       (00:24:15)
[INFO]       AutoMut:  Mutating residue number 265 from chain A (leucine) into glutamic acid       (00:24:15)
[INFO]       AutoMut:  Mutating residue number 265 from chain A (leucine) into aspartic acid       (00:24:25)
[INFO]       AutoMut:  Mutating residue number 372 from chain A (leucine) into arginine            (00:24:52)
[INFO]       AutoMut:  Mutating residue number 265 from chain A (leucine) into lysine              (00:24:53)
[INFO]       AutoMut:  Mutating residue number 265 from chain A (leucine) into arginine            (00:25:05)
[INFO]       AutoMut:  Mutating residue number 76 from chain A (isoleucine) into glutamic acid     (00:25:38)
[INFO]       AutoMut:  Mutating residue number 76 from chain A (isoleucine) into aspartic acid     (00:25:38)
[INFO]       AutoMut:  Mutating residue number 536 from chain A (isoleucine) into glutamic acid    (00:25:45)
[INFO]       AutoMut:  Mutating residue number 76 from chain A (isoleucine) into arginine          (00:26:23)
[INFO]       AutoMut:  Mutating residue number 536 from chain A (isoleucine) into lysine           (00:26:24)
[INFO]       AutoMut:  Mutating residue number 76 from chain A (isoleucine) into lysine            (00:26:25)
[INFO]       AutoMut:  Mutating residue number 536 from chain A (isoleucine) into aspartic acid    (00:27:23)
[INFO]       AutoMut:  Mutating residue number 11 from chain A (leucine) into glutamic acid        (00:27:24)
[INFO]       AutoMut:  Mutating residue number 11 from chain A (leucine) into aspartic acid        (00:27:29)
[INFO]       AutoMut:  Mutating residue number 536 from chain A (isoleucine) into arginine         (00:28:02)
[INFO]       AutoMut:  Mutating residue number 11 from chain A (leucine) into lysine               (00:28:03)
[INFO]       AutoMut:  Mutating residue number 11 from chain A (leucine) into arginine             (00:28:08)
[INFO]       AutoMut:  Effect of mutation residue number 169 from chain B (isoleucine) into        
                       glutamic acid: Energy difference: -0.1720 kcal/mol, Difference in average   
                       score from the base case: -0.0130                                           (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 169 from chain B (isoleucine) into        
                       lysine: Energy difference: 0.0169 kcal/mol, Difference in average score     
                       from the base case: -0.0106                                                 (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 169 from chain B (isoleucine) into        
                       aspartic acid: Energy difference: -0.1926 kcal/mol, Difference in average   
                       score from the base case: -0.0130                                           (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 169 from chain B (isoleucine) into        
                       arginine: Energy difference: 0.0462 kcal/mol, Difference in average score   
                       from the base case: -0.0131                                                 (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 372 from chain A (leucine) into glutamic  
                       acid: Energy difference: 0.3567 kcal/mol, Difference in average score from  
                       the base case: -0.0120                                                      (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 372 from chain A (leucine) into lysine:   
                       Energy difference: -0.0700 kcal/mol, Difference in average score from the   
                       base case: -0.0117                                                          (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 372 from chain A (leucine) into aspartic  
                       acid: Energy difference: 1.3456 kcal/mol, Difference in average score from  
                       the base case: -0.0116                                                      (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 372 from chain A (leucine) into arginine: 
                       Energy difference: -0.5927 kcal/mol, Difference in average score from the   
                       base case: -0.0116                                                          (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 265 from chain A (leucine) into glutamic  
                       acid: Energy difference: 0.5124 kcal/mol, Difference in average score from  
                       the base case: -0.0093                                                      (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 265 from chain A (leucine) into lysine:   
                       Energy difference: 0.2816 kcal/mol, Difference in average score from the    
                       base case: -0.0090                                                          (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 265 from chain A (leucine) into aspartic  
                       acid: Energy difference: 0.8488 kcal/mol, Difference in average score from  
                       the base case: -0.0093                                                      (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 265 from chain A (leucine) into arginine: 
                       Energy difference: 0.3949 kcal/mol, Difference in average score from the    
                       base case: -0.0093                                                          (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 76 from chain A (isoleucine) into         
                       glutamic acid: Energy difference: 0.8881 kcal/mol, Difference in average    
                       score from the base case: -0.0105                                           (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 76 from chain A (isoleucine) into lysine: 
                       Energy difference: -0.6321 kcal/mol, Difference in average score from the   
                       base case: -0.0099                                                          (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 76 from chain A (isoleucine) into         
                       aspartic acid: Energy difference: 0.3486 kcal/mol, Difference in average    
                       score from the base case: -0.0102                                           (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 76 from chain A (isoleucine) into         
                       arginine: Energy difference: -0.5279 kcal/mol, Difference in average score  
                       from the base case: -0.0105                                                 (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 536 from chain A (isoleucine) into        
                       glutamic acid: Energy difference: 0.1090 kcal/mol, Difference in average    
                       score from the base case: -0.0113                                           (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 536 from chain A (isoleucine) into        
                       lysine: Energy difference: -0.1310 kcal/mol, Difference in average score    
                       from the base case: -0.0107                                                 (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 536 from chain A (isoleucine) into        
                       aspartic acid: Energy difference: 0.4253 kcal/mol, Difference in average    
                       score from the base case: -0.0124                                           (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 536 from chain A (isoleucine) into        
                       arginine: Energy difference: -0.4101 kcal/mol, Difference in average score  
                       from the base case: -0.0119                                                 (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain A (leucine) into glutamic   
                       acid: Energy difference: 0.4979 kcal/mol, Difference in average score from  
                       the base case: -0.0111                                                      (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain A (leucine) into lysine:    
                       Energy difference: 0.2227 kcal/mol, Difference in average score from the    
                       base case: -0.0111                                                          (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain A (leucine) into aspartic   
                       acid: Energy difference: 0.2842 kcal/mol, Difference in average score from  
                       the base case: -0.0115                                                      (00:28:54)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain A (leucine) into arginine:  
                       Energy difference: 0.1334 kcal/mol, Difference in average score from the    
                       base case: -0.0114                                                          (00:28:54)
[INFO]       Main:     Simulation completed successfully.                                          (00:29:05)

Note Your prediction results will be deleted after 360 days.

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Show buried residues

Minimal score value: -3.3493
Maximal score value: 1.7115
Average score: -0.6524
Total score value: -713.0292

Download the table (csv)

The table below lists A4D score for protein residues. Residues with A4D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan4D score

1	E	A	-2.2381
2	V	A	-1.4424
3	Q	A	-1.4896
4	L	A	0.0000
5	V	A	0.6874
6	E	A	0.0000
7	S	A	-0.2527
8	G	A	-0.6197
9	G	A	0.0565
10	G	A	0.8130
11	L	A	1.2812
12	V	A	-0.2473
13	K	A	-1.8389
14	P	A	-1.7845
15	G	A	-1.3815
16	G	A	-1.0718
17	S	A	-1.1881
18	L	A	-1.0904
19	K	A	-2.1318
20	L	A	0.0000
21	S	A	-0.4111
22	C	A	0.0000
23	A	A	0.5129
24	A	A	0.0000
25	S	A	-0.6736
26	G	A	-1.4370
27	Y	A	-0.7425
28	T	A	-0.0781
29	F	A	0.0000
30	T	A	-0.0089
31	S	A	0.0000
32	Y	A	0.4310
33	V	A	0.0000
34	M	A	0.0000
35	H	A	0.0000
36	W	A	0.0000
37	V	A	0.0000
38	R	A	0.0000
39	Q	A	-0.6358
40	A	A	-1.0835
41	P	A	-0.8902
42	G	A	-1.4476
43	K	A	-2.1217
44	G	A	-1.1440
45	L	A	0.0000
46	E	A	-0.6209
47	W	A	0.0000
48	I	A	0.0000
49	G	A	0.0000
50	Y	A	0.0000
51	I	A	0.0000
52	N	A	-0.7947
53	P	A	0.0000
54	Y	A	-0.3060
55	N	A	-1.5954
56	D	A	-2.1305
57	G	A	-1.5170
58	T	A	-0.9750
59	K	A	-0.9395
60	Y	A	-1.1795
61	N	A	0.0000
62	E	A	-3.3493
63	K	A	-3.1598
64	F	A	0.0000
65	Q	A	-2.6912
66	G	A	-1.6428
67	R	A	-1.4542
68	V	A	0.0000
69	T	A	-0.9186
70	I	A	0.0000
71	S	A	-0.7964
72	S	A	-0.6391
73	D	A	-0.8757
74	K	A	-0.8575
75	S	A	0.0429
76	I	A	1.3415
77	S	A	0.2518
78	T	A	0.0000
79	A	A	0.0000
80	Y	A	-0.6536
81	M	A	0.0000
82	E	A	-1.6361
83	L	A	0.0000
84	S	A	-1.0338
85	S	A	-1.0212
86	L	A	0.0000
87	R	A	-2.3850
88	S	A	-2.1269
89	E	A	-2.4460
90	D	A	0.0000
91	T	A	-0.5101
92	A	A	0.0000
93	M	A	0.4631
94	Y	A	0.0000
95	Y	A	0.0000
96	C	A	0.0000
97	A	A	0.0000
98	R	A	0.0000
99	G	A	0.0000
100	T	A	0.1278
101	Y	A	0.3947
102	Y	A	0.2760
103	Y	A	0.0938
104	G	A	0.0000
105	T	A	-0.3468
106	R	A	-0.5803
107	V	A	0.0000
108	F	A	0.0000
109	D	A	0.0000
110	Y	A	-0.1335
111	W	A	0.0000
112	G	A	0.0000
113	Q	A	-1.1073
114	G	A	0.0000
115	T	A	0.3730
116	L	A	1.1794
117	V	A	0.0000
118	T	A	0.1610
119	V	A	0.0000
120	S	A	-1.0972
121	S	A	-1.5129
122	G	A	-1.2358
123	G	A	-1.1697
124	G	A	-1.1929
125	G	A	-1.2444
126	S	A	-1.0194
127	G	A	-1.6116
128	G	A	-1.1955
129	G	A	-1.3739
130	G	A	-1.2032
131	S	A	-1.0277
132	G	A	-1.4496
133	G	A	-1.3951
134	G	A	-1.2931
135	G	A	-1.7921
136	S	A	-1.2569
137	D	A	-1.6384
138	I	A	0.0000
139	V	A	1.0222
140	M	A	0.0000
141	T	A	0.2685
142	Q	A	0.0000
143	S	A	-0.2212
144	P	A	-0.1047
145	A	A	-0.1220
146	T	A	-0.4835
147	L	A	-0.1308
148	S	A	-0.3230
149	L	A	0.0000
150	S	A	-0.6588
151	P	A	-0.9658
152	G	A	-1.2306
153	E	A	-1.3577
154	R	A	-1.4238
155	A	A	0.0000
156	T	A	-0.2161
157	L	A	0.0000
158	S	A	0.0000
159	C	A	0.0000
160	R	A	-0.3777
161	S	A	0.0000
162	S	A	-0.6004
163	K	A	-1.9421
164	S	A	-1.1559
165	L	A	0.0000
166	Q	A	-1.1651
167	N	A	0.0000
168	V	A	-0.7965
169	N	A	-0.8069
170	G	A	-1.1501
171	N	A	-0.7894
172	T	A	0.0000
173	Y	A	0.0000
174	L	A	0.0000
175	Y	A	0.0000
176	W	A	0.0000
177	F	A	0.0000
178	Q	A	0.0000
179	Q	A	0.0000
180	K	A	-1.3779
181	P	A	-0.9118
182	G	A	-1.2950
183	Q	A	-1.7539
184	S	A	-1.2354
185	P	A	0.0000
186	Q	A	-0.9106
187	L	A	0.0000
188	L	A	0.0000
189	I	A	0.0000
190	Y	A	-0.4838
191	R	A	-0.8497
192	M	A	-0.3830
193	S	A	-0.4261
194	N	A	-0.7199
195	L	A	-0.2577
196	N	A	0.0000
197	S	A	-0.4892
198	G	A	-0.7775
199	V	A	-0.3082
200	P	A	-0.6295
201	D	A	-0.8089
202	R	A	-0.7104
203	F	A	0.0000
204	S	A	0.0000
205	G	A	0.0000
206	S	A	0.0000
207	G	A	0.0000
208	S	A	0.0000
209	G	A	0.0000
210	T	A	-0.8054
211	E	A	0.0000
212	F	A	0.0000
213	T	A	0.0000
214	L	A	0.0000
215	T	A	0.0000
216	I	A	0.0000
217	S	A	0.0000
218	S	A	-1.1179
219	L	A	0.0000
220	E	A	-1.9800
221	P	A	-1.4719
222	E	A	-2.2837
223	D	A	0.0000
224	F	A	-0.6854
225	A	A	0.0000
226	V	A	-0.3099
227	Y	A	0.0000
228	Y	A	0.0000
229	C	A	0.0000
230	M	A	0.0000
231	Q	A	0.0000
232	H	A	0.0000
233	L	A	-1.0390
234	E	A	-2.0440
235	Y	A	-1.1653
236	P	A	-1.3473
237	I	A	0.0000
238	T	A	-0.0857
239	F	A	0.0000
240	G	A	0.0000
241	A	A	-0.2331
242	G	A	0.0000
243	T	A	0.0000
244	K	A	-0.6987
245	L	A	0.0000
246	E	A	-0.4346
247	I	A	0.9994
248	K	A	-0.3285
249	S	A	-0.4505
250	G	A	-0.8821
251	G	A	-1.1992
252	G	A	-1.3047
253	G	A	-1.4559
254	S	A	-1.2135
255	E	A	-1.4931
256	V	A	-0.7070
257	Q	A	-0.6485
258	L	A	0.0000
259	V	A	1.1943
260	E	A	0.0000
261	S	A	-0.1370
262	G	A	-0.7699
263	G	A	0.1218
264	G	A	0.6572
265	L	A	1.3804
266	V	A	0.0000
267	Q	A	-1.9077
268	P	A	-2.1272
269	G	A	-2.1758
270	R	A	-2.7187
271	S	A	-2.0833
272	L	A	-1.4283
273	R	A	-2.0982
274	L	A	0.0000
275	S	A	-0.3767
276	C	A	0.0000
277	A	A	-0.0545
278	A	A	0.0000
279	S	A	-0.6160
280	G	A	-0.7044
281	F	A	-0.2806
282	T	A	-0.3013
283	F	A	0.0000
284	D	A	-1.3346
285	D	A	0.0000
286	Y	A	0.0000
287	A	A	0.0000
288	M	A	0.0000
289	H	A	0.0000
290	W	A	0.0000
291	V	A	0.0000
292	R	A	0.0000
293	Q	A	-0.6470
294	A	A	-1.0290
295	P	A	-0.8478
296	G	A	-1.4553
297	K	A	-2.3893
298	G	A	-1.5892
299	L	A	0.0000
300	E	A	-1.0384
301	W	A	0.0000
302	V	A	0.0000
303	S	A	0.0000
304	G	A	0.0000
305	I	A	0.0000
306	S	A	0.0000
307	W	A	-0.6046
308	N	A	-1.2996
309	S	A	-0.7675
310	G	A	-0.6037
311	S	A	0.0756
312	I	A	0.9457
313	G	A	0.2166
314	Y	A	-0.6166
315	A	A	0.0000
316	D	A	-2.7924
317	S	A	-1.8660
318	V	A	0.0000
319	K	A	-2.5377
320	G	A	-1.6460
321	R	A	0.0000
322	F	A	0.0000
323	T	A	-0.6600
324	I	A	0.0000
325	S	A	-0.1926
326	R	A	-1.0740
327	D	A	-1.5645
328	N	A	-1.7793
329	A	A	-1.4026
330	K	A	-2.3519
331	N	A	-1.8860
332	S	A	0.0000
333	L	A	0.0000
334	Y	A	-0.5369
335	L	A	0.0000
336	Q	A	-1.2164
337	M	A	0.0000
338	N	A	-1.8625
339	S	A	-1.7508
340	L	A	0.0000
341	R	A	-2.5489
342	A	A	-1.8732
343	E	A	-2.3154
344	D	A	0.0000
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281	L	B	0.0000
282	S	B	0.0000
283	C	B	0.0000
284	A	B	0.0000
285	V	B	0.0000
286	K	B	-0.7179
287	G	B	-1.2125
288	F	B	0.0000
289	Y	B	-1.1932
290	P	B	0.0000
291	S	B	-0.3374
292	D	B	-0.9316
293	I	B	-0.3814
294	A	B	0.0000
295	V	B	0.0000
296	E	B	-0.5080
297	W	B	0.0000
298	E	B	-0.9936
299	S	B	0.0000
300	N	B	-1.4697
301	G	B	-1.5844
302	Q	B	-1.1591
303	P	B	-0.7624
304	E	B	-0.9240
305	N	B	-0.6131
306	N	B	-0.3864
307	Y	B	-0.2577
308	K	B	-0.3061
309	T	B	-0.1505
310	T	B	0.0000
311	P	B	0.0941
312	P	B	0.0000
313	V	B	0.0000
314	L	B	-0.5475
315	D	B	-1.1662
316	S	B	-1.5100
317	D	B	-2.0303
318	G	B	-1.1021
319	S	B	0.0000
320	F	B	0.0000
321	F	B	0.0000
322	L	B	0.0000
323	V	B	0.0000
324	S	B	0.0000
325	K	B	0.0000
326	L	B	0.0000
327	T	B	-0.4986
328	V	B	0.0000
329	D	B	-2.3418
330	K	B	-2.4276
331	S	B	-2.1872
332	R	B	-1.9689
333	W	B	0.0000
334	Q	B	-2.3704
335	Q	B	-2.1294
336	G	B	-1.1826
337	N	B	-0.8573
338	V	B	0.2789
339	F	B	0.0000
340	S	B	0.0000
341	C	B	0.0000
342	S	B	0.0000
343	V	B	0.0000
344	M	B	0.0000
345	H	B	0.0000
346	E	B	-1.0510
347	A	B	-1.5317
348	L	B	0.0000
349	H	B	-1.8571
350	N	B	-1.5050
351	H	B	-0.9737
352	Y	B	-0.5724
353	T	B	0.0000
354	Q	B	-0.9949
355	K	B	-0.8725
356	S	B	-0.3606
357	L	B	0.0000
358	S	B	-0.1170
359	L	B	-0.3757
360	S	B	-0.6139
361	P	B	-1.2322
362	G	B	-1.4296
363	K	B	-2.0240

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Automated mutations analysis - charged mutations

In the automated mutations mode, the server selects aggregation prone resides and each selected residue is mutated to glutamic acid, lysine, aspartic acid and arginine. The table below shows 2 best scored mutants for each mutated residue. Protein variants are ordered according to the mutation effect they had on protein stability (energetic effect) together with the difference in the average per-residue aggregation score between the wild type and the mutant (in the table green values indicate a positive change, grey are neutral, and orange/red mean destabilizing or more aggregation prone mutants).
Summary for all the mutants can be found in this CSV file .

Mutant	Energetic effect	Score comparison
LR372A	-0.5927	-0.0116	View	CSV	PDB
IK76A	-0.6321	-0.0099	View	CSV	PDB
IR536A	-0.4101	-0.0119	View	CSV	PDB
IR76A	-0.5279	-0.0105	View	CSV	PDB
ID169B	-0.1926	-0.013	View	CSV	PDB
IE169B	-0.172	-0.013	View	CSV	PDB
IK536A	-0.131	-0.0107	View	CSV	PDB
LK372A	-0.07	-0.0117	View	CSV	PDB
LR11A	0.1334	-0.0114	View	CSV	PDB
LK11A	0.2227	-0.0111	View	CSV	PDB
LK265A	0.2816	-0.009	View	CSV	PDB
LR265A	0.3949	-0.0093	View	CSV	PDB

Project name: 7cc8956f57d549b

Status: done

Started: 2026-05-20 08:57:31

Automated mutations analysis - charged mutations

Mutant

Energetic effect

Score comparison