Aggrescan4D: E75K17_K0

Project name: E75K17_K0_H0

Status: done

Started: 2026-05-29 06:29:30

Chain sequence(s)	A: EVQLVESGGGLVKPGGSLKLSCAASGYTFTSYVMHWVRQAPGKGLEWIGYINPYNDGTKYNEKFQGRVTISSDKSISTAYMELSSLRSEDTAMYYCARGTYYYGTRVFDYWGQGTLVTVSSGGGGSGGGGSGGGGSDIVMTQSPATLSLSPGERATLSCRSSKSLQNVNGNTYLYWFQQKPGQSPQLLIYRMSNLNSGVPDRFSGSGSGTEFTLTISSLEPEDFAVYYCMQHLEYPITFGAGTKLEIKSGGGGSEVQLVESGGALVKPGGSLRLSCAASGFTFTEAWMHWVRQAPGKQLEWVAQIKDRSQTYATYYAESVKGRFTISRDDSKNTLYLQMNSLKTEDTAVYYCRGVYYANAPFDYWGQGTLVTVSSGGGGSGGGGSGGGGSDIVMTQSPLSLPVTPGEPASISCRSSQSLVHNTGNTYLSWYLQKPGQSPQSLIYKVSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCGQGTQAPFTFGSGTKVEIKGGGGDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLYITREPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALAAPIEKTISKAKGQPREPQVYTLPPCREEMTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK B: DVQLVESGGGLVQAGGSLRLSCRASGHTFSDTAVGWFRRVPGKEREFVATIAGSGSVRYSESVKGRFTISGDNAKNMVYLQMNRLKPEDTGVYYCAAAVGISYDYWGQGTQVTVSSEPKSSDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLYITREPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALAAPIEKTISKAKGQPREPQVCTLPPSREEMTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK input PDB
Selected Chain(s)	A,B
Distance of aggregation	10 Å
FoldX usage	Yes
pH calculations	No
alphaCutter usage	No
Dynamic mode	No
Automated mutations	Yes
Downloads	Download all the data

Simulation log

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[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:01)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:01)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:01)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:01)
[INFO]       FoldX:    Starting FoldX energy minimization                                          (00:00:01)
[INFO]       Analysis: Starting Aggrescan4D on folded.pdb                                          (00:22:05)
[INFO]       AutoMutEv:Residue number 370 from chain A and a score of 1.550 (leucine) selected for 
                       automated mutation                                                          (00:22:23)
[INFO]       AutoMutEv:Residue number 101 from chain A and a score of 1.514 (tyrosine) selected    
                       for automated mutation                                                      (00:22:23)
[INFO]       AutoMutEv:Residue number 539 from chain A and a score of 1.449 (isoleucine) selected  
                       for automated mutation                                                      (00:22:23)
[INFO]       AutoMutEv:Residue number 265 from chain A and a score of 1.417 (leucine) selected for 
                       automated mutation                                                          (00:22:23)
[INFO]       AutoMutEv:Residue number 11 from chain A and a score of 1.256 (leucine) selected for  
                       automated mutation                                                          (00:22:23)
[INFO]       AutoMutEv:Residue number 76 from chain A and a score of 1.177 (isoleucine) selected   
                       for automated mutation                                                      (00:22:23)
[INFO]       AutoMutEv:Mutating residue number 370 from chain A (leucine) into methionine          (00:22:23)
[INFO]       AutoMutEv:Mutating residue number 101 from chain A (tyrosine) into histidine          (00:22:23)
[INFO]       AutoMutEv:Mutating residue number 101 from chain A (tyrosine) into cysteine           (00:22:23)
[INFO]       AutoMutEv:Mutating residue number 101 from chain A (tyrosine) into tryptophan         (00:23:10)
[INFO]       AutoMutEv:Mutating residue number 539 from chain A (isoleucine) into threonine        (00:23:18)
[INFO]       AutoMutEv:Mutating residue number 539 from chain A (isoleucine) into methionine       (00:23:52)
[INFO]       AutoMutEv:Mutating residue number 539 from chain A (isoleucine) into leucine          (00:24:02)
[INFO]       AutoMutEv:Mutating residue number 265 from chain A (leucine) into methionine          (00:24:07)
[INFO]       AutoMutEv:Mutating residue number 11 from chain A (leucine) into methionine           (00:24:37)
[INFO]       AutoMutEv:Mutating residue number 76 from chain A (isoleucine) into threonine         (00:24:49)
[INFO]       AutoMutEv:Mutating residue number 76 from chain A (isoleucine) into methionine        (00:24:50)
[INFO]       AutoMutEv:Mutating residue number 76 from chain A (isoleucine) into leucine           (00:25:21)
[INFO]       AutoMutEv:Effect of mutation residue number 370 from chain A (leucine) into           
                       methionine: Energy difference: -0.4233 kcal/mol, Difference in average      
                       score from the base case: -0.0033                                           (00:26:02)
[INFO]       AutoMutEv:Effect of mutation residue number 101 from chain A (tyrosine) into          
                       histidine: Energy difference: 0.2462 kcal/mol, Difference in average score  
                       from the base case: -0.0054                                                 (00:26:02)
[INFO]       AutoMutEv:Effect of mutation residue number 101 from chain A (tyrosine) into          
                       cysteine: Energy difference: 0.3913 kcal/mol, Difference in average score   
                       from the base case: -0.0027                                                 (00:26:02)
[INFO]       AutoMutEv:Effect of mutation residue number 101 from chain A (tyrosine) into          
                       tryptophan: Energy difference: -0.8040 kcal/mol, Difference in average      
                       score from the base case: -0.0029                                           (00:26:02)
[INFO]       AutoMutEv:Effect of mutation residue number 539 from chain A (isoleucine) into        
                       threonine: Energy difference: 0.0929 kcal/mol, Difference in average score  
                       from the base case: -0.0076                                                 (00:26:02)
[INFO]       AutoMutEv:Effect of mutation residue number 539 from chain A (isoleucine) into        
                       methionine: Energy difference: -0.7583 kcal/mol, Difference in average      
                       score from the base case: -0.0020                                           (00:26:02)
[INFO]       AutoMutEv:Effect of mutation residue number 539 from chain A (isoleucine) into        
                       leucine: Energy difference: -0.4909 kcal/mol, Difference in average score   
                       from the base case: -0.0011                                                 (00:26:02)
[INFO]       AutoMutEv:Effect of mutation residue number 265 from chain A (leucine) into           
                       methionine: Energy difference: 0.0224 kcal/mol, Difference in average score 
                       from the base case: -0.0016                                                 (00:26:02)
[INFO]       AutoMutEv:Effect of mutation residue number 11 from chain A (leucine) into            
                       methionine: Energy difference: 0.0078 kcal/mol, Difference in average score 
                       from the base case: -0.0016                                                 (00:26:02)
[INFO]       AutoMutEv:Effect of mutation residue number 76 from chain A (isoleucine) into         
                       threonine: Energy difference: 1.0343 kcal/mol, Difference in average score  
                       from the base case: -0.0057                                                 (00:26:02)
[INFO]       AutoMutEv:Effect of mutation residue number 76 from chain A (isoleucine) into         
                       methionine: Energy difference: 0.4396 kcal/mol, Difference in average score 
                       from the base case: -0.0024                                                 (00:26:02)
[INFO]       AutoMutEv:Effect of mutation residue number 76 from chain A (isoleucine) into         
                       leucine: Energy difference: 0.6092 kcal/mol, Difference in average score    
                       from the base case: -0.0007                                                 (00:26:02)
[INFO]       Main:     Simulation completed successfully.                                          (00:26:16)

Note Your prediction results will be deleted after 360 days.

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Show buried residues

Minimal score value: -3.3714
Maximal score value: 1.5498
Average score: -0.7202
Total score value: -778.5251

Download the table (csv)

The table below lists A4D score for protein residues. Residues with A4D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan4D score

1	E	A	-2.5619
2	V	A	-1.3159
3	Q	A	-1.5771
4	L	A	0.0000
5	V	A	-0.0127
6	E	A	0.0000
7	S	A	-0.4450
8	G	A	-0.6351
9	G	A	0.0269
10	G	A	0.7499
11	L	A	1.2560
12	V	A	-0.3180
13	K	A	-1.9088
14	P	A	-1.9463
15	G	A	-1.4320
16	G	A	-1.1161
17	S	A	-1.2155
18	L	A	-1.0464
19	K	A	-2.0962
20	L	A	0.0000
21	S	A	-0.5371
22	C	A	0.0000
23	A	A	0.2520
24	A	A	0.0000
25	S	A	-0.7636
26	G	A	-1.5541
27	Y	A	0.0000
28	T	A	-0.3453
29	F	A	0.0000
30	T	A	-0.2512
31	S	A	0.3615
32	Y	A	0.0000
33	V	A	0.0000
34	M	A	0.0000
35	H	A	0.0000
36	W	A	0.0000
37	V	A	0.0000
38	R	A	0.0000
39	Q	A	-0.6365
40	A	A	-1.0968
41	P	A	-1.0815
42	G	A	-1.4494
43	K	A	-2.1225
44	G	A	-1.0874
45	L	A	0.0000
46	E	A	-0.5722
47	W	A	0.0000
48	I	A	0.0000
49	G	A	0.0000
50	Y	A	-0.2700
51	I	A	0.0000
52	N	A	0.0000
53	P	A	0.0000
54	Y	A	0.0531
55	N	A	-1.5610
56	D	A	-2.3718
57	G	A	-1.4126
58	T	A	-1.0598
59	K	A	-1.0357
60	Y	A	-1.2090
61	N	A	0.0000
62	E	A	-3.3714
63	K	A	-3.1571
64	F	A	0.0000
65	Q	A	-2.7596
66	G	A	-1.6938
67	R	A	-1.5519
68	V	A	0.0000
69	T	A	-0.9101
70	I	A	0.0000
71	S	A	-0.8622
72	S	A	-0.7753
73	D	A	-1.1821
74	K	A	-1.3502
75	S	A	-0.2108
76	I	A	1.1775
77	S	A	0.0383
78	T	A	0.0000
79	A	A	0.0000
80	Y	A	-0.6541
81	M	A	0.0000
82	E	A	-1.5804
83	L	A	0.0000
84	S	A	-1.0881
85	S	A	-1.0728
86	L	A	0.0000
87	R	A	-2.7098
88	S	A	-2.2374
89	E	A	-2.4677
90	D	A	0.0000
91	T	A	-0.6053
92	A	A	0.0000
93	M	A	0.4607
94	Y	A	0.0000
95	Y	A	0.0000
96	C	A	0.0000
97	A	A	0.0000
98	R	A	0.0000
99	G	A	0.0000
100	T	A	0.7226
101	Y	A	1.5145
102	Y	A	0.8077
103	Y	A	0.6661
104	G	A	0.0000
105	T	A	0.3719
106	R	A	0.0499
107	V	A	0.0000
108	F	A	0.0000
109	D	A	0.0000
110	Y	A	0.1203
111	W	A	-0.2723
112	G	A	0.0000
113	Q	A	-1.2767
114	G	A	0.0000
115	T	A	0.2547
116	L	A	1.1481
117	V	A	0.0000
118	T	A	0.1030
119	V	A	0.0000
120	S	A	-0.8509
121	S	A	-1.5707
122	G	A	-1.5292
123	G	A	-1.1413
124	G	A	-1.2154
125	G	A	-1.2171
126	S	A	-1.0700
127	G	A	-1.1900
128	G	A	-1.2137
129	G	A	-1.2087
130	G	A	-1.1995
131	S	A	-1.0723
132	G	A	-1.3930
133	G	A	-1.4408
134	G	A	-1.2875
135	G	A	-1.7857
136	S	A	-1.1850
137	D	A	-1.4954
138	I	A	0.0000
139	V	A	0.8339
140	M	A	0.0000
141	T	A	-0.5784
142	Q	A	-0.7107
143	S	A	-0.6889
144	P	A	-0.3550
145	A	A	-0.2769
146	T	A	-0.4286
147	L	A	-0.1202
148	S	A	-0.2690
149	L	A	-0.4532
150	S	A	0.0000
151	P	A	0.0000
152	G	A	0.0000
153	E	A	-1.6496
154	R	A	-2.1193
155	A	A	0.0000
156	T	A	-0.5856
157	L	A	0.0000
158	S	A	-1.0497
159	C	A	0.0000
160	R	A	-2.7018
161	S	A	0.0000
162	S	A	-1.2112
163	K	A	-2.4419
164	S	A	-1.9501
165	L	A	0.0000
166	Q	A	-1.3722
167	N	A	-0.5151
168	V	A	0.9049
169	N	A	-0.4769
170	G	A	-0.6280
171	N	A	0.0000
172	T	A	0.0000
173	Y	A	0.0000
174	L	A	0.0000
175	Y	A	0.0000
176	W	A	0.0000
177	F	A	0.0000
178	Q	A	0.0000
179	Q	A	0.0000
180	K	A	-1.2512
181	P	A	-0.8339
182	G	A	-1.3029
183	Q	A	-1.7779
184	S	A	-1.1765
185	P	A	0.0000
186	Q	A	-0.7982
187	L	A	0.0000
188	L	A	0.0000
189	I	A	0.0000
190	Y	A	0.0000
191	R	A	0.0000
192	M	A	-0.2680
193	S	A	-0.5042
194	N	A	-0.5516
195	L	A	-0.1237
196	N	A	0.0000
197	S	A	-0.4617
198	G	A	-0.7144
199	V	A	-0.4938
200	P	A	-0.9290
201	D	A	-1.8512
202	R	A	0.0000
203	F	A	0.0000
204	S	A	-0.5431
205	G	A	0.0000
206	S	A	-0.9957
207	G	A	-1.2141
208	S	A	-1.4451
209	G	A	-1.6978
210	T	A	-2.3127
211	E	A	-2.9090
212	F	A	0.0000
213	T	A	-0.8895
214	L	A	0.0000
215	T	A	-0.5338
216	I	A	0.0000
217	S	A	-1.1706
218	S	A	0.0000
219	L	A	0.0000
220	E	A	0.0000
221	P	A	-1.0736
222	E	A	-2.0590
223	D	A	0.0000
224	F	A	-0.5463
225	A	A	0.0000
226	V	A	-0.1640
227	Y	A	0.0000
228	Y	A	0.0000
229	C	A	0.0000
230	M	A	0.0000
231	Q	A	0.0000
232	H	A	0.0000
233	L	A	-0.9090
234	E	A	-1.7044
235	Y	A	-0.9876
236	P	A	-1.2308
237	I	A	0.0000
238	T	A	0.0718
239	F	A	0.3803
240	G	A	0.0000
241	A	A	-0.2569
242	G	A	0.0000
243	T	A	0.0000
244	K	A	-0.7704
245	L	A	0.0000
246	E	A	-0.2392
247	I	A	0.4787
248	K	A	-1.1034
249	S	A	-0.8634
250	G	A	-1.0246
251	G	A	0.0000
252	G	A	-1.0371
253	G	A	0.0000
254	S	A	0.0000
255	E	A	-1.4434
256	V	A	-0.4235
257	Q	A	-0.8860
258	L	A	0.0000
259	V	A	0.0714
260	E	A	0.0000
261	S	A	-0.3718
262	G	A	-0.5415
263	G	A	0.2706
264	A	A	0.6470
265	L	A	1.4166
266	V	A	-0.1664
267	K	A	-1.8039
268	P	A	-1.7598
269	G	A	-1.4095
270	G	A	-1.0580
271	S	A	-1.3564
272	L	A	-1.0900
273	R	A	-2.0786
274	L	A	0.0000
275	S	A	-0.5254
276	C	A	0.0000
277	A	A	-0.2653
278	A	A	0.0000
279	S	A	0.0000
280	G	A	0.0000
281	F	A	0.0000
282	T	A	-1.2091
283	F	A	0.0000
284	T	A	-1.6400
285	E	A	-1.7163
286	A	A	0.0000
287	W	A	-0.0486
288	M	A	0.0000
289	H	A	0.0000
290	W	A	0.0000
291	V	A	0.0000
292	R	A	0.0000
293	Q	A	-0.7303
294	A	A	-1.1126
295	P	A	-0.8832
296	G	A	-1.5156
297	K	A	-2.3531
298	Q	A	-1.5857
299	L	A	0.0000
300	E	A	-0.9990
301	W	A	0.0000
302	V	A	0.0000
303	A	A	0.0000
304	Q	A	0.0000
305	I	A	0.0000
306	K	A	-0.6543
307	D	A	0.0000
308	R	A	-2.3360
309	S	A	-1.6115
310	Q	A	-1.4158
311	T	A	-0.9785
312	Y	A	-0.5138
313	A	A	-0.2304
314	T	A	0.2593
315	Y	A	0.1500
316	Y	A	-0.6469
317	A	A	0.0000
318	E	A	-2.7048
319	S	A	-2.0611
320	V	A	0.0000
321	K	A	-2.5935
322	G	A	-1.7977
323	R	A	-1.6906
324	F	A	0.0000
325	T	A	-0.8102
326	I	A	0.0000
327	S	A	-0.2901
328	R	A	-0.8589
329	D	A	-1.1889
330	D	A	-1.5161
331	S	A	-1.4771
332	K	A	-1.9422
333	N	A	0.0000
334	T	A	-0.6900
335	L	A	0.0000
336	Y	A	-0.5495
337	L	A	0.0000
338	Q	A	-1.2669
339	M	A	0.0000
340	N	A	-1.4881
341	S	A	-1.2514
342	L	A	0.0000
343	K	A	-2.0852
344	T	A	-1.7875
345	E	A	-2.2743
346	D	A	0.0000
347	T	A	-0.3401
348	A	A	0.0000
349	V	A	0.7376
350	Y	A	0.0000
351	Y	A	0.0000
352	C	A	0.0000
353	R	A	0.0000
354	G	A	0.0000
355	V	A	0.0000
356	Y	A	0.1948
357	Y	A	0.1530
358	A	A	-0.4490
359	N	A	-1.1028
360	A	A	-0.4225
361	P	A	-0.0324
362	F	A	0.4860
363	D	A	0.0000
364	Y	A	0.1555
365	W	A	-0.2378
366	G	A	0.0000
367	Q	A	-1.2883
368	G	A	0.0000
369	T	A	0.4985
370	L	A	1.5498
371	V	A	0.0000
372	T	A	0.3780
373	V	A	0.0000
374	S	A	-0.7234
375	S	A	-1.4522
376	G	A	-1.3756
377	G	A	-1.1371
378	G	A	-1.2145
379	G	A	-1.2210
380	S	A	-1.0644
381	G	A	-1.1878
382	G	A	-1.3900
383	G	A	-1.2055
384	G	A	-1.4154
385	S	A	-1.0912
386	G	A	-1.4276
387	G	A	-1.6484
388	G	A	-1.2898
389	G	A	-1.5982
390	S	A	-1.1303
391	D	A	-1.2316
392	I	A	0.0000
393	V	A	0.8936
394	M	A	0.0000
395	T	A	-0.3528
396	Q	A	0.0000
397	S	A	-0.2137
398	P	A	0.2420
399	L	A	0.9667
400	S	A	-0.0830
401	L	A	-0.3967
402	P	A	-1.1119
403	V	A	0.0000
404	T	A	-1.3928
405	P	A	0.0000
406	G	A	0.0000
407	E	A	-1.6330
408	P	A	-1.4335
409	A	A	0.0000
410	S	A	-0.8807
411	I	A	0.0000
412	S	A	-0.9181
413	C	A	0.0000
414	R	A	-2.3808
415	S	A	0.0000
416	S	A	-0.9242
417	Q	A	-1.5488
418	S	A	-0.9507
419	L	A	0.0000
420	V	A	0.1641
421	H	A	-0.7761
422	N	A	-1.3762
423	T	A	-0.9292
424	G	A	-0.8493
425	N	A	-0.7294
426	T	A	-0.2466
427	Y	A	-0.1115
428	L	A	0.0000
429	S	A	0.0000
430	W	A	0.0000
431	Y	A	0.0000
432	L	A	0.0000
433	Q	A	-1.1206
434	K	A	-1.7596
435	P	A	-1.1184
436	G	A	-1.5013
437	Q	A	-2.1599
438	S	A	-1.4667
439	P	A	0.0000
440	Q	A	-1.1123
441	S	A	0.0000
442	L	A	0.0000
443	I	A	0.0000
444	Y	A	-0.3802
445	K	A	-0.5700
446	V	A	0.0000
447	S	A	-0.9110
448	N	A	-1.2482
449	R	A	-1.5614
450	A	A	-0.7590
451	S	A	-0.8447
452	G	A	-1.1311
453	V	A	0.0000
454	P	A	-1.4317
455	D	A	-2.5304
456	R	A	-1.6831
457	F	A	0.0000
458	S	A	-1.2657
459	G	A	0.0000
460	S	A	-0.8323
461	G	A	-1.1333
462	S	A	-0.8121
463	G	A	-0.7843
464	T	A	-1.5710
465	D	A	-2.2798
466	F	A	0.0000
467	T	A	-1.0569
468	L	A	0.0000
469	K	A	-1.2865
470	I	A	0.0000
471	S	A	-1.8333
472	R	A	-1.7382
473	V	A	0.0000
474	E	A	-1.0993
475	A	A	-1.1124
476	E	A	-1.7071
477	D	A	0.0000
478	V	A	-0.8940
479	G	A	0.0000
480	V	A	-0.3670
481	Y	A	0.0000
482	Y	A	0.0000
483	C	A	0.0000
484	G	A	0.0000
485	Q	A	0.0000
486	G	A	-0.3703
487	T	A	0.0000
488	Q	A	-1.3055
489	A	A	-0.5750
490	P	A	-0.8216
491	F	A	-0.1584
492	T	A	-0.0562
493	F	A	0.0000
494	G	A	0.0000
495	S	A	-0.2749
496	G	A	0.0000
497	T	A	0.0000
498	K	A	-0.8197
499	V	A	0.0000
500	E	A	-1.7417
501	I	A	-1.5208
502	K	A	-2.1269
503	G	A	-2.1387
504	G	A	-1.8608
505	G	A	-2.0238
506	G	A	-2.4007
507	D	A	-3.2578
508	K	A	-3.0588
509	T	A	-2.0094
510	H	A	-2.1049
511	T	A	-1.0734
512	C	A	-0.3732
513	P	A	-0.4311
514	P	A	-0.1520
515	C	A	0.0248
516	P	A	-0.5845
517	A	A	-0.4682
518	P	A	-0.7365
519	E	A	-0.8608
520	A	A	-0.2475
521	A	A	-0.4418
522	G	A	-0.4959
523	G	A	0.0000
524	P	A	0.0000
525	S	A	0.0000
526	V	A	0.0000
527	F	A	0.0000
528	L	A	0.0000
529	F	A	0.1638
530	P	A	0.0000
531	P	A	0.0000
532	K	A	-1.7905
533	P	A	-0.9190
534	K	A	-1.0067
535	D	A	-1.3365
536	T	A	0.0000
537	L	A	0.0000
538	Y	A	0.3811
539	I	A	1.4491
540	T	A	0.1390
541	R	A	-1.4447
542	E	A	-2.0972
543	P	A	0.0000
544	E	A	-1.4746
545	V	A	0.0000
546	T	A	0.0000
547	C	A	0.0000
548	V	A	0.0000
549	V	A	0.0000
550	V	A	0.0000
551	D	A	-0.9478
552	V	A	0.0000
553	S	A	-2.0960
554	H	A	-2.4027
555	E	A	-2.8381
556	D	A	-2.3517
557	P	A	-2.5016
558	E	A	-3.0501
559	V	A	-1.9889
560	K	A	-2.2476
561	F	A	-1.4170
562	N	A	-1.1228
563	W	A	0.0000
564	Y	A	-0.5401
565	V	A	-0.7940
566	D	A	-2.0729
567	G	A	-0.8429
568	V	A	0.6303
569	E	A	-0.5901
570	V	A	-0.5458
571	H	A	-1.8709
572	N	A	-2.1718
573	A	A	-1.8837
574	K	A	-2.4167
575	T	A	-1.9423
576	K	A	-2.1514
577	P	A	-2.2540
578	R	A	-3.3516
579	E	A	-3.0582
580	E	A	-3.0356
581	Q	A	-1.4568
582	Y	A	-0.2571
583	N	A	-1.0837
584	S	A	-1.1078
585	T	A	-1.7294
586	Y	A	-2.1992
587	R	A	-1.8482
588	V	A	0.0000
589	V	A	0.0000
590	S	A	0.0000
591	V	A	0.0000
592	L	A	0.0000
593	T	A	-1.0658
594	V	A	0.0000
595	L	A	0.4359
596	H	A	0.0000
597	Q	A	-0.9675
598	D	A	-1.2483
599	W	A	0.0000
600	L	A	-0.8747
601	N	A	-1.9558
602	G	A	-1.9651
603	K	A	-2.0135
604	E	A	-1.9299
605	Y	A	0.0000
606	K	A	-1.3318
607	C	A	0.0000
608	K	A	-1.4035
609	V	A	0.0000
610	S	A	-1.4700
611	N	A	0.0000
612	K	A	-2.4045
613	A	A	-1.2685
614	L	A	-0.4779
615	A	A	-0.1765
616	A	A	-0.5508
617	P	A	-0.9786
618	I	A	0.0000
619	E	A	-2.0167
620	K	A	-1.1329
621	T	A	-1.0034
622	I	A	-0.5154
623	S	A	-1.3705
624	K	A	0.0000
625	A	A	-1.5265
626	K	A	-2.5928
627	G	A	-1.9565
628	Q	A	-2.1141
629	P	A	-1.6844
630	R	A	-1.9833
631	E	A	-2.5558
632	P	A	0.0000
633	Q	A	-1.2122
634	V	A	0.0000
635	Y	A	0.0000
636	T	A	-0.6973
637	L	A	0.0000
638	P	A	-0.3168
639	P	A	0.0000
640	C	A	-1.2644
641	R	A	-2.4501
642	E	A	-2.4509
643	E	A	0.0000
644	M	A	-1.8203
645	T	A	-1.6410
646	K	A	-2.3539
647	N	A	-2.5257
648	Q	A	-2.2483
649	V	A	0.0000
650	S	A	0.0000
651	L	A	0.0000
652	W	A	0.0000
653	C	A	0.0000
654	L	A	0.0000
655	V	A	0.0000
656	K	A	-0.6209
657	G	A	-1.1542
658	F	A	0.0000
659	Y	A	-1.1491
660	P	A	0.0000
661	S	A	-0.1298
662	D	A	-0.6678
663	I	A	-0.3385
664	A	A	0.0000
665	V	A	0.0000
666	E	A	-1.3435
667	W	A	0.0000
668	E	A	-1.7320
669	S	A	0.0000
670	N	A	-1.8228
671	G	A	-1.7336
672	Q	A	-2.2469
673	P	A	-1.8885
674	E	A	0.0000
675	N	A	-1.9116
676	N	A	-1.1702
677	Y	A	-0.8291
678	K	A	-0.8170
679	T	A	-0.3752
680	T	A	0.0000
681	P	A	0.0000
682	P	A	0.0000
683	V	A	0.2220
684	L	A	0.0308
685	D	A	-0.8446
686	S	A	-1.6173
687	D	A	-2.0427
688	G	A	-1.1493
689	S	A	0.0000
690	F	A	0.0000
691	F	A	0.0000
692	L	A	0.0000
693	Y	A	0.0000
694	S	A	0.0000
695	K	A	0.0000
696	L	A	0.0000
697	T	A	-0.7368
698	V	A	0.0000
699	D	A	-2.2442
700	K	A	-2.4402
701	S	A	-2.1112
702	R	A	-1.8731
703	W	A	0.0000
704	Q	A	-2.3248
705	Q	A	-2.0859
706	G	A	-1.1718
707	N	A	-0.8195
708	V	A	0.2623
709	F	A	0.0000
710	S	A	0.0000
711	C	A	0.0000
712	S	A	0.0000
713	V	A	0.0000
714	M	A	0.0000
715	H	A	0.0000
716	E	A	-0.9891
717	A	A	-1.4768
718	L	A	-1.4319
719	H	A	-1.7770
720	N	A	-1.6552
721	H	A	-1.1592
722	Y	A	-0.4496
723	T	A	-0.6206
724	Q	A	-0.8927
725	K	A	-0.9116
726	S	A	-0.4007
727	L	A	0.0000
728	S	A	-0.1266
729	L	A	-0.3761
730	S	A	-0.5982
731	P	A	-1.0985
732	G	A	-1.4333
733	K	A	-2.0276
1	D	B	-2.0999
2	V	B	0.0000
3	Q	B	-1.9781
4	L	B	0.0000
5	V	B	-0.5929
6	E	B	0.0000
7	S	B	-0.2798
8	G	B	0.0000
9	G	B	0.0000
10	G	B	0.0000
11	L	B	0.0000
12	V	B	0.0000
13	Q	B	-1.4442
14	A	B	0.0000
15	G	B	0.0000
16	G	B	-0.8040
17	S	B	-0.6508
18	L	B	0.0000
19	R	B	-0.5080
20	L	B	0.0000
21	S	B	0.0000
22	C	B	0.0000
23	R	B	-1.0432
24	A	B	0.0000
25	S	B	-1.3820
26	G	B	-1.6512
27	H	B	-1.5179
28	T	B	-0.9051
29	F	B	0.0000
30	S	B	-1.1731
31	D	B	-1.8237
32	T	B	0.0000
33	A	B	-0.1858
34	V	B	0.0000
35	G	B	0.0000
36	W	B	0.0000
37	F	B	0.0000
38	R	B	0.0000
39	R	B	-0.8709
40	V	B	-1.2092
41	P	B	-1.2569
42	G	B	-1.5043
43	K	B	-2.3668
44	E	B	-1.7028
45	R	B	0.0000
46	E	B	-0.9277
47	F	B	0.0000
48	V	B	0.0000
49	A	B	0.0000
50	T	B	0.0000
51	I	B	0.0000
52	A	B	-0.3842
53	G	B	-0.8092
54	S	B	-0.6514
55	G	B	0.0000
56	S	B	-0.2421
57	V	B	0.0000
58	R	B	-0.4694
59	Y	B	-0.5468
60	S	B	-1.0175
61	E	B	-2.2734
62	S	B	-1.4817
63	V	B	0.0000
64	K	B	-1.4090
65	G	B	-1.0377
66	R	B	0.0000
67	F	B	0.0000
68	T	B	-0.2565
69	I	B	0.0000
70	S	B	-0.1319
71	G	B	0.0000
72	D	B	0.0000
73	N	B	-0.4864
74	A	B	-0.6929
75	K	B	-0.9722
76	N	B	-0.9470
77	M	B	0.0000
78	V	B	0.0000
79	Y	B	0.0000
80	L	B	0.0000
81	Q	B	-0.5781
82	M	B	0.0000
83	N	B	-0.6277
84	R	B	0.0000
85	L	B	0.0000
86	K	B	-1.2828
87	P	B	-1.4207
88	E	B	-1.9893
89	D	B	0.0000
90	T	B	-0.6664
91	G	B	0.0000
92	V	B	-0.1528
93	Y	B	0.0000
94	Y	B	0.0000
95	C	B	0.0000
96	A	B	0.0000
97	A	B	0.0000
98	A	B	0.0000
99	V	B	0.2225
100	G	B	0.0919
101	I	B	0.9371
102	S	B	0.0000
103	Y	B	0.0000
104	D	B	-0.3122
105	Y	B	-0.2884
106	W	B	-0.4715
107	G	B	0.0000
108	Q	B	-1.2563
109	G	B	-0.6053
110	T	B	0.0000
111	Q	B	-0.3437
112	V	B	0.0000
113	T	B	0.0000
114	V	B	0.0000
115	S	B	0.0000
116	S	B	-1.8939
117	E	B	-2.7085
118	P	B	-2.2553
119	K	B	-2.7363
120	S	B	-2.1646
121	S	B	-2.0609
122	D	B	-3.2336
123	K	B	-2.9270
124	T	B	-2.0760
125	H	B	-1.7996
126	T	B	-1.2134
127	C	B	-0.4544
128	P	B	-0.3719
129	P	B	-0.6826
130	C	B	-0.0430
131	P	B	-0.4763
132	A	B	-0.8356
133	P	B	-1.0690
134	E	B	-1.9260
135	A	B	-0.9267
136	A	B	-0.5516
137	G	B	-0.7791
138	G	B	-0.6040
139	P	B	0.0000
140	S	B	-0.0953
141	V	B	0.0000
142	F	B	0.0000
143	L	B	0.0000
144	F	B	0.0216
145	P	B	0.0000
146	P	B	0.0000
147	K	B	-1.9507
148	P	B	-0.9575
149	K	B	-1.2192
150	D	B	-1.7625
151	T	B	0.0000
152	L	B	0.0000
153	Y	B	-0.5309
154	I	B	-0.5245
155	T	B	-0.8060
156	R	B	-2.1628
157	E	B	-2.9057
158	P	B	0.0000
159	E	B	-1.7952
160	V	B	0.0000
161	T	B	-0.3242
162	C	B	0.0000
163	V	B	0.0000
164	V	B	0.0000
165	V	B	0.0000
166	D	B	-0.6701
167	V	B	0.0000
168	S	B	-2.0139
169	H	B	-2.4530
170	E	B	-2.9795
171	D	B	-2.5676
172	P	B	-2.5912
173	E	B	-3.0871
174	V	B	-2.0257
175	K	B	-2.2592
176	F	B	-1.3046
177	N	B	-1.0073
178	W	B	0.0000
179	Y	B	-0.4764
180	V	B	-0.7616
181	D	B	-2.0392
182	G	B	-0.8008
183	V	B	0.7109
184	E	B	-0.4305
185	V	B	-0.4301
186	H	B	-1.8169
187	N	B	-2.4144
188	A	B	-1.8870
189	K	B	-2.3990
190	T	B	-1.8506
191	K	B	-1.9235
192	P	B	-1.8336
193	R	B	-2.5318
194	E	B	-2.3431
195	E	B	-2.1329
196	Q	B	0.0000
197	Y	B	-0.7146
198	N	B	-0.6107
199	S	B	-0.8344
200	T	B	-1.3541
201	Y	B	0.0000
202	R	B	-1.4178
203	V	B	0.0000
204	V	B	0.0000
205	S	B	0.0000
206	V	B	0.0000
207	L	B	0.0000
208	T	B	-1.6583
209	V	B	0.0000
210	L	B	-0.5994
211	H	B	-0.8332
212	Q	B	-0.8288
213	D	B	-1.2038
214	W	B	0.0000
215	L	B	-0.7981
216	N	B	-1.8624
217	G	B	-1.9135
218	K	B	-2.1949
219	E	B	-1.9607
220	Y	B	0.0000
221	K	B	-1.2853
222	C	B	0.0000
223	K	B	-1.1018
224	V	B	0.0000
225	S	B	-1.3314
226	N	B	0.0000
227	K	B	-2.4643
228	A	B	-1.4878
229	L	B	-0.3605
230	A	B	-0.1284
231	A	B	-0.1674
232	P	B	-0.6384
233	I	B	-0.1739
234	E	B	-1.1319
235	K	B	-0.7667
236	T	B	-0.9716
237	I	B	0.0000
238	S	B	-1.2160
239	K	B	0.0000
240	A	B	0.0000
241	K	B	-2.4615
242	G	B	-2.0053
243	Q	B	-2.1524
244	P	B	-1.7626
245	R	B	-2.0891
246	E	B	-2.6166
247	P	B	0.0000
248	Q	B	-1.1210
249	V	B	0.0000
250	C	B	0.0000
251	T	B	0.0000
252	L	B	0.0000
253	P	B	-0.3705
254	P	B	-0.8207
255	S	B	-1.4214
256	R	B	-2.5314
257	E	B	-2.4722
258	E	B	0.0000
259	M	B	-1.7421
260	T	B	-1.4560
261	K	B	-1.9154
262	N	B	-2.3301
263	Q	B	-2.1117
264	V	B	0.0000
265	S	B	0.0000
266	L	B	0.0000
267	S	B	0.0000
268	C	B	0.0000
269	A	B	0.0000
270	V	B	0.0000
271	K	B	-0.7562
272	G	B	-1.2589
273	F	B	0.0000
274	Y	B	-1.2913
275	P	B	0.0000
276	S	B	0.0000
277	D	B	-0.5954
278	I	B	-0.2769
279	A	B	-0.4258
280	V	B	0.0000
281	E	B	-1.1784
282	W	B	0.0000
283	E	B	-1.5365
284	S	B	0.0000
285	N	B	-1.7680
286	G	B	-1.6579
287	Q	B	-2.2313
288	P	B	-1.8431
289	E	B	0.0000
290	N	B	-2.1886
291	N	B	-1.4626
292	Y	B	-0.8478
293	K	B	-0.8487
294	T	B	-0.3146
295	T	B	0.0000
296	P	B	-0.0831
297	P	B	-0.1533
298	V	B	0.0000
299	L	B	0.0000
300	D	B	-1.1762
301	S	B	-1.3899
302	D	B	-2.0888
303	G	B	-1.2721
304	S	B	0.0000
305	F	B	0.0000
306	F	B	0.0000
307	L	B	0.0000
308	V	B	0.0000
309	S	B	0.0000
310	K	B	0.0000
311	L	B	0.0000
312	T	B	-0.7853
313	V	B	0.0000
314	D	B	-2.2241
315	K	B	-2.4676
316	S	B	-2.1585
317	R	B	-1.8959
318	W	B	0.0000
319	Q	B	-2.3369
320	Q	B	-2.0986
321	G	B	-1.1661
322	N	B	-0.8439
323	V	B	0.2363
324	F	B	0.0000
325	S	B	0.0000
326	C	B	0.0000
327	S	B	0.0000
328	V	B	0.0000
329	M	B	0.0000
330	H	B	0.0000
331	E	B	-0.9151
332	A	B	-1.4246
333	L	B	0.0000
334	H	B	-1.2623
335	N	B	-0.3003
336	H	B	-0.7290
337	Y	B	-0.2668
338	T	B	-0.5690
339	Q	B	-0.7796
340	K	B	-0.8370
341	S	B	-0.4161
342	L	B	0.0000
343	S	B	-0.1181
344	L	B	-0.3250
345	S	B	-0.5703
346	P	B	-1.0832
347	G	B	-1.4239
348	K	B	-2.0278

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Automated mutations analysis - evolutionary conserved mutations

In the automated mutations mode, the server selects aggregation prone resides and each selected residue is mutated based off an evolutionary approach. The table below shows 2 best scored mutants for each mutated residue. Protein variants are ordered according to the mutation effect they had on protein stability (energetic effect) together with the difference in the average per-residue aggregation score between the wild type and the mutant (in the table green values indicate a positive change, grey are neutral, and orange/red mean destabilizing or more aggregation prone mutants).
Summary for all the mutants can be found in this CSV file .

Mutant	Energetic effect	Score comparison
YW101A	-0.804	-0.0029	View	CSV	PDB
LM370A	-0.4233	-0.0033	View	CSV	PDB
IM539A	-0.7583	-0.002	View	CSV	PDB
IL539A	-0.4909	-0.0011	View	CSV	PDB
LM11A	0.0078	-0.0016	View	CSV	PDB
LM265A	0.0224	-0.0016	View	CSV	PDB
YH101A	0.2462	-0.0054	View	CSV	PDB
IT76A	1.0343	-0.0057	View	CSV	PDB
IM76A	0.4396	-0.0024	View	CSV	PDB

Project name: E75K17_K0_H0

Status: done

Started: 2026-05-29 06:29:30

Automated mutations analysis - evolutionary conserved mutations

Mutant

Energetic effect

Score comparison