Project name: kiana project

Status: done

Started: 2026-07-29 10:07:33
Chain sequence(s) A: HHHHHHSSGAFSADTPEIVYTHDTGLDYITYSDYELDPANPLAGGAAWIEGAFVPPSEARISIFDQGFYTSDATYTTFHVWNGNAFRLGDHIERLFSNAESIRLIPPLTQDEVKEIALELVAKTELREAMVTVTITRGYSSTPFERDITKHRPQVYMSACPYQWIVPFDRIRDGVHLMVAQSVRRTPRSSIDPQVKNFQWGDLIRAIQETHDRGFELPLLLDCDNLLAEGPGFNVVVIKDGVVRSPGRAALPGITRKTVLEIAESLGHEAILADITPAELYDADEVLGCSTGGGVWPFVSVDGNSISDGVPGPVTQSIIRRYWELNVEPSSLLTPVQY
B: HHHHHHSSGAFSADTPEIVYTHDTGLDYITYSDYELDPANPLAGGAAWIEGAFVPPSEARISIFDQGFYTSDATYTTFHVWNGNAFRLGDHIERLFSNAESIRLIPPLTQDEVKEIALELVAKTELREAMVTVTITRGYSSTPFERDITKHRPQVYMSACPYQWIVPFDRIRDGVHLMVAQSVRRTPRSSIDPQVKNFQWGDLIRAIQETHDRGFELPLLLDCDNLLAEGPGFNVVVIKDGVVRSPGRAALPGITRKTVLEIAESLGHEAILADITPAELYDADEVLGCSTGGGVWPFVSVDGNSISDGVPGPVTQSIIRRYWELNVEPSSLLTPVQY
input PDB
Selected Chain(s) A,B
Distance of aggregation 5 Å
FoldX usage Yes
pH calculations No
alphaCutter usage No
Dynamic mode No
Automated mutations Yes
Downloads Download all the data
Simulation log 
[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:01)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:01)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:01)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:01)
[INFO]       FoldX:    Starting FoldX energy minimization                                          (00:00:02)
[INFO]       Analysis: Starting Aggrescan4D on folded.pdb                                          (00:07:05)
[INFO]       AutoMutEv:Residue number 105 from chain A and a score of 2.013 (isoleucine) selected  
                       for automated mutation                                                      (00:07:10)
[INFO]       AutoMutEv:Residue number 105 from chain B and a score of 2.013 (isoleucine) selected  
                       for automated mutation                                                      (00:07:10)
[INFO]       AutoMutEv:Residue number 19 from chain B and a score of 1.921 (valine) selected for   
                       automated mutation                                                          (00:07:10)
[INFO]       AutoMutEv:Residue number 19 from chain A and a score of 1.883 (valine) selected for   
                       automated mutation                                                          (00:07:10)
[INFO]       AutoMutEv:Residue number 310 from chain A and a score of 1.744 (valine) selected for  
                       automated mutation                                                          (00:07:10)
[INFO]       AutoMutEv:Residue number 310 from chain B and a score of 1.743 (valine) selected for  
                       automated mutation                                                          (00:07:10)
[INFO]       AutoMutEv:Mutating residue number 105 from chain A (isoleucine) into threonine        (00:07:10)
[INFO]       AutoMutEv:Mutating residue number 105 from chain A (isoleucine) into leucine          (00:07:10)
[INFO]       AutoMutEv:Mutating residue number 105 from chain B (isoleucine) into methionine       (00:07:10)
[INFO]       AutoMutEv:Mutating residue number 105 from chain A (isoleucine) into methionine       (00:07:24)
[INFO]       AutoMutEv:Mutating residue number 105 from chain B (isoleucine) into threonine        (00:07:24)
[INFO]       AutoMutEv:Mutating residue number 105 from chain B (isoleucine) into leucine          (00:07:26)
[INFO]       AutoMutEv:Mutating residue number 19 from chain B (valine) into threonine             (00:07:38)
[INFO]       AutoMutEv:Mutating residue number 19 from chain B (valine) into methionine            (00:07:40)
[INFO]       AutoMutEv:Mutating residue number 19 from chain A (valine) into alanine               (00:07:41)
[INFO]       AutoMutEv:Mutating residue number 19 from chain B (valine) into alanine               (00:07:51)
[INFO]       AutoMutEv:Mutating residue number 19 from chain A (valine) into methionine            (00:07:54)
[INFO]       AutoMutEv:Mutating residue number 19 from chain A (valine) into threonine             (00:07:57)
[INFO]       AutoMutEv:Mutating residue number 310 from chain A (valine) into threonine            (00:08:08)
[INFO]       AutoMutEv:Mutating residue number 310 from chain A (valine) into methionine           (00:08:09)
[INFO]       AutoMutEv:Mutating residue number 310 from chain B (valine) into alanine              (00:08:10)
[INFO]       AutoMutEv:Mutating residue number 310 from chain A (valine) into alanine              (00:08:22)
[INFO]       AutoMutEv:Mutating residue number 310 from chain B (valine) into methionine           (00:08:24)
[INFO]       AutoMutEv:Mutating residue number 310 from chain B (valine) into threonine            (00:08:26)
[INFO]       AutoMutEv:Effect of mutation residue number 105 from chain A (isoleucine) into        
                       threonine: Energy difference: 0.5766 kcal/mol, Difference in average score  
                       from the base case: -0.0031                                                 (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 105 from chain A (isoleucine) into        
                       methionine: Energy difference: -0.0532 kcal/mol, Difference in average      
                       score from the base case: -0.0015                                           (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 105 from chain A (isoleucine) into        
                       leucine: Energy difference: -0.0630 kcal/mol, Difference in average score   
                       from the base case: -0.0004                                                 (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 105 from chain B (isoleucine) into        
                       threonine: Energy difference: 0.5226 kcal/mol, Difference in average score  
                       from the base case: -0.0031                                                 (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 105 from chain B (isoleucine) into        
                       methionine: Energy difference: 0.0326 kcal/mol, Difference in average score 
                       from the base case: -0.0015                                                 (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 105 from chain B (isoleucine) into        
                       leucine: Energy difference: 0.0367 kcal/mol, Difference in average score    
                       from the base case: -0.0004                                                 (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 19 from chain B (valine) into threonine:  
                       Energy difference: -0.0102 kcal/mol, Difference in average score from the   
                       base case: -0.0037                                                          (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 19 from chain B (valine) into alanine:    
                       Energy difference: 0.0932 kcal/mol, Difference in average score from the    
                       base case: -0.0042                                                          (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 19 from chain B (valine) into methionine: 
                       Energy difference: 0.2432 kcal/mol, Difference in average score from the    
                       base case: -0.0014                                                          (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 19 from chain A (valine) into threonine:  
                       Energy difference: -0.3112 kcal/mol, Difference in average score from the   
                       base case: -0.0037                                                          (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 19 from chain A (valine) into alanine:    
                       Energy difference: 0.0126 kcal/mol, Difference in average score from the    
                       base case: -0.0034                                                          (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 19 from chain A (valine) into methionine: 
                       Energy difference: 0.0248 kcal/mol, Difference in average score from the    
                       base case: -0.0015                                                          (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 310 from chain A (valine) into threonine: 
                       Energy difference: 0.2329 kcal/mol, Difference in average score from the    
                       base case: -0.0038                                                          (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 310 from chain A (valine) into alanine:   
                       Energy difference: 0.4585 kcal/mol, Difference in average score from the    
                       base case: -0.0036                                                          (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 310 from chain A (valine) into            
                       methionine: Energy difference: -0.4440 kcal/mol, Difference in average      
                       score from the base case: -0.0000                                           (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 310 from chain B (valine) into threonine: 
                       Energy difference: 0.0884 kcal/mol, Difference in average score from the    
                       base case: -0.0038                                                          (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 310 from chain B (valine) into alanine:   
                       Energy difference: 0.4661 kcal/mol, Difference in average score from the    
                       base case: -0.0036                                                          (00:08:41)
[INFO]       AutoMutEv:Effect of mutation residue number 310 from chain B (valine) into            
                       methionine: Energy difference: -0.6250 kcal/mol, Difference in average      
                       score from the base case: -0.0005                                           (00:08:41)
[INFO]       Main:     Simulation completed successfully.                                          (00:08:47)
Show buried residues
Minimal score value
-2.2693
Maximal score value
2.0126
Average score
-0.292
Total score value
-197.4065
Download the table (csv)

The table below lists A4D score for protein residues. Residues with A4D score > 0.0000 are marked by yellow rows.

residue index residue name chain Aggrescan4D score mutation
1 H A -1.1707
2 H A -1.3513
3 H A -1.3535
4 H A -1.3559
5 H A -1.2527
6 H A -0.6514
7 S A -0.3108
8 S A -0.2269
9 G A -0.4785
10 A A 0.0776
11 F A 0.6696
12 S A -0.0797
13 A A -0.3521
14 D A -1.7908
15 T A -0.3809
16 P A -0.4864
17 E A -1.7628
18 I A 0.4520
19 V A 1.8833
20 Y A 0.4603
21 T A -0.1883
22 H A -1.3262
23 D A -1.9720
24 T A 0.0000
25 G A -0.4307
26 L A -0.2089
27 D A -1.7068
28 Y A -0.0633
29 I A 0.0000
30 T A -0.0424
31 Y A 0.1357
32 S A -0.3327
33 D A -1.7558
34 Y A -0.4361
35 E A -1.7500
36 L A -0.4241
37 D A -1.4100
38 P A -0.4944
39 A A -0.0387
40 N A -0.3221
41 P A -0.3064
42 L A 0.0000
43 A A 0.0120
44 G A 0.0000
45 G A 0.0000
46 A A 0.0000
47 A A 0.0000
48 W A 0.1686
49 I A 0.0000
50 E A -1.1264
51 G A -0.6482
52 A A 0.0278
53 F A 0.4764
54 V A 0.3030
55 P A -0.0001
56 P A -0.0724
57 S A -0.5304
58 E A -1.8555
59 A A 0.0000
60 R A -0.5597
61 I A 0.0000
62 S A 0.0000
63 I A 0.0000
64 F A 0.0000
65 D A 0.0000
66 Q A -0.1067
67 G A 0.0000
68 F A 0.0000
69 Y A 0.1206
70 T A 0.0155
71 S A 0.0000
72 D A 0.0000
73 A A 0.0000
74 T A 0.0000
75 Y A 0.1268
76 T A 0.0000
77 T A -0.0148
78 F A 0.0000
79 H A 0.0000
80 V A 0.0000
81 W A 0.0000
82 N A -0.5223
83 G A 0.0000
84 N A -0.2542
85 A A 0.0000
86 F A 0.0000
87 R A -0.1825
88 L A 0.0000
89 G A -0.4038
90 D A -0.6005
91 H A 0.0000
92 I A 0.0000
93 E A -1.7594
94 R A -0.5492
95 L A 0.0000
96 F A 0.0000
97 S A -0.1449
98 N A 0.0000
99 A A 0.0000
100 E A -2.0659
101 S A -0.4770
102 I A -0.0215
103 R A -1.9882
104 L A 0.0000
105 I A 2.0126
106 P A 0.0000
107 P A -0.2139
108 L A 0.1721
109 T A -0.1265
110 Q A -0.9336
111 D A -2.1233
112 E A -1.5782
113 V A 0.0000
114 K A -0.7304
115 E A -1.7252
116 I A -0.1316
117 A A 0.0000
118 L A 0.0000
119 E A -1.1204
120 L A 0.0000
121 V A 0.0000
122 A A -0.1048
123 K A -0.8269
124 T A 0.0000
125 E A -0.2969
126 L A 0.0000
127 R A -0.5039
128 E A 0.0000
129 A A 0.0000
130 M A 0.0000
131 V A 0.0000
132 T A -0.0095
133 V A 0.0000
134 T A 0.0000
135 I A 0.0000
136 T A 0.0000
137 R A -0.1957
138 G A 0.0000
139 Y A 0.1426
140 S A 0.0000
141 S A -0.0741
142 T A -0.0351
143 P A -0.0434
144 F A 0.0000
145 E A -0.9109
146 R A -2.0567
147 D A -0.8887
148 I A 0.0999
149 T A -0.3365
150 K A -1.7332
151 H A -0.5487
152 R A -0.8428
153 P A -0.2427
154 Q A -0.1296
155 V A 0.0000
156 Y A 0.0000
157 M A 0.0000
158 S A 0.0000
159 A A 0.0000
160 C A 0.0000
161 P A -0.0246
162 Y A 0.0000
163 Q A -0.1240
164 W A 0.2141
165 I A 0.4000
166 V A 0.0000
167 P A -0.1056
168 F A -0.1216
169 D A -1.7863
170 R A -1.1884
171 I A 0.0000
172 R A -1.9256
173 D A -2.1502
174 G A 0.0000
175 V A 0.0000
176 H A -0.1979
177 L A 0.0000
178 M A 0.0000
179 V A 0.0000
180 A A 0.0000
181 Q A -1.2365
182 S A -0.4278
183 V A 0.0000
184 R A -0.3943
185 R A -0.2470
186 T A 0.0000
187 P A -0.2717
188 R A -1.3191
189 S A -0.4361
190 S A 0.0000
191 I A 0.0000
192 D A -0.3981
193 P A 0.0000
194 Q A -0.3769
195 V A 0.0000
196 K A -0.3123
197 N A -0.2104
198 F A 0.0000
199 Q A 0.0000
200 W A 0.0000
201 G A -0.0574
202 D A 0.0000
203 L A 0.0000
204 I A 0.0000
205 R A -0.5954
206 A A 0.0000
207 I A 0.2568
208 Q A -0.0977
209 E A 0.0000
210 T A 0.0000
211 H A -1.1500
212 D A -2.2693
213 R A -2.2493
214 G A -0.7139
215 F A 0.2820
216 E A -0.4678
217 L A 0.0000
218 P A 0.0000
219 L A 0.0000
220 L A 0.0000
221 L A 0.0000
222 D A -0.0837
223 C A 0.3737
224 D A -1.8582
225 N A -1.3922
226 L A 0.0634
227 L A 0.0000
228 A A 0.0000
229 E A 0.0000
230 G A 0.0000
231 P A 0.0000
232 G A -0.1107
233 F A 0.0000
234 N A 0.0000
235 V A 0.0000
236 V A 0.0000
237 V A 0.0000
238 I A 0.0000
239 K A -0.9166
240 D A -1.9390
241 G A -0.4831
242 V A 0.3644
243 V A 0.0000
244 R A -0.2533
245 S A 0.0000
246 P A 0.0000
247 G A -0.4834
248 R A -1.8940
249 A A -0.3282
250 A A 0.0000
251 L A 0.0000
252 P A -0.1619
253 G A -0.0852
254 I A 0.0000
255 T A 0.0000
256 R A 0.0000
257 K A -1.0637
258 T A 0.0000
259 V A 0.0000
260 L A -0.1519
261 E A -1.7891
262 I A 0.0000
263 A A 0.0000
264 E A -1.8585
265 S A -0.4423
266 L A 0.4361
267 G A -0.4096
268 H A -0.6519
269 E A -1.8615
270 A A -0.0986
271 I A 1.4777
272 L A 1.4398
273 A A 0.0317
274 D A -1.1002
275 I A 0.0000
276 T A -0.0358
277 P A -0.0322
278 A A -0.0459
279 E A -0.5329
280 L A 0.0000
281 Y A 0.0579
282 D A -1.7215
283 A A 0.0000
284 D A -0.6247
285 E A 0.0000
286 V A 0.0000
287 L A 0.0000
288 G A 0.0000
289 C A 0.0000
290 S A -0.0276
291 T A -0.0212
292 G A -0.0510
293 G A 0.0000
294 G A 0.0000
295 V A 0.0000
296 W A 0.0000
297 P A 0.0000
298 F A 0.0000
299 V A 0.0000
300 S A -0.0521
301 V A 0.0000
302 D A -0.7734
303 G A -0.7340
304 N A -0.9231
305 S A -0.3613
306 I A 0.0000
307 S A -0.5312
308 D A -1.8502
309 G A -0.1303
310 V A 1.7438
311 P A 0.2724
312 G A -0.1174
313 P A -0.2350
314 V A 0.1305
315 T A 0.0000
316 Q A -1.2131
317 S A -0.2985
318 I A 0.0000
319 I A 0.0000
320 R A -1.1493
321 R A -0.8968
322 Y A 0.0000
323 W A 0.0000
324 E A -0.9812
325 L A 0.0119
326 N A 0.0000
327 V A 0.8929
328 E A -0.9522
329 P A -0.4762
330 S A -0.2170
331 S A -0.1917
332 L A 0.2222
333 L A 0.2135
334 T A -0.0361
335 P A -0.2900
336 V A 0.0000
337 Q A -0.9607
338 Y A 1.0997
1 H B -1.1705
2 H B -1.3531
3 H B -1.3555
4 H B -1.3561
5 H B -1.3577
6 H B -1.2137
7 S B -0.4348
8 S B -0.3379
9 G B -0.4945
10 A B 0.0736
11 F B 0.5246
12 S B -0.1066
13 A B -0.3545
14 D B -1.7896
15 T B -0.4033
16 P B -0.5936
17 E B -1.7439
18 I B 0.6666
19 V B 1.9209
20 Y B 0.4530
21 T B -0.1095
22 H B -0.9281
23 D B -1.8991
24 T B 0.0000
25 G B -0.4367
26 L B -0.2412
27 D B -1.7142
28 Y B -0.0721
29 I B 0.0000
30 T B -0.0450
31 Y B 0.1221
32 S B -0.3346
33 D B -1.7546
34 Y B -0.4309
35 E B -1.7472
36 L B -0.3985
37 D B -1.3232
38 P B -0.4784
39 A B -0.0366
40 N B -0.3087
41 P B -0.3038
42 L B 0.0000
43 A B 0.0124
44 G B 0.0000
45 G B 0.0000
46 A B 0.0000
47 A B 0.0000
48 W B 0.0000
49 I B 0.0000
50 E B -0.4004
51 G B -0.2822
52 A B 0.0771
53 F B 0.4963
54 V B 0.3115
55 P B -0.0027
56 P B -0.0828
57 S B -0.5451
58 E B -1.8580
59 A B 0.0000
60 R B -0.6239
61 I B 0.0000
62 S B -0.1108
63 I B 0.0000
64 F B 0.0000
65 D B 0.0000
66 Q B -0.1186
67 G B 0.0000
68 F B 0.0000
69 Y B 0.1939
70 T B 0.0302
71 S B 0.0000
72 D B 0.0000
73 A B 0.0000
74 T B 0.0000
75 Y B 0.0000
76 T B 0.0000
77 T B -0.0104
78 F B 0.0000
79 H B 0.0000
80 V B 0.0000
81 W B 0.0000
82 N B -1.2730
83 G B 0.0000
84 N B -0.2302
85 A B 0.0000
86 F B 0.0000
87 R B -0.1945
88 L B 0.0000
89 G B -0.5240
90 D B -0.9292
91 H B 0.0000
92 I B 0.0000
93 E B -1.9835
94 R B -1.2462
95 L B 0.0000
96 F B 0.0000
97 S B -0.1946
98 N B 0.0000
99 A B 0.0000
100 E B -2.0605
101 S B -0.4748
102 I B -0.0100
103 R B -1.9810
104 L B 0.0000
105 I B 2.0126
106 P B 0.0000
107 P B -0.2226
108 L B 0.1233
109 T B -0.1443
110 Q B -0.9802
111 D B -1.9756
112 E B -0.7067
113 V B 0.0000
114 K B -0.7614
115 E B -1.8981
116 I B 0.0000
117 A B 0.0000
118 L B 0.0000
119 E B -0.8278
120 L B 0.0000
121 V B 0.0000
122 A B -0.1048
123 K B -0.7971
124 T B 0.0000
125 E B -0.2976
126 L B 0.0000
127 R B -0.5199
128 E B 0.0000
129 A B 0.0000
130 M B 0.0000
131 V B 0.0000
132 T B 0.0000
133 V B 0.0000
134 T B 0.0000
135 I B 0.0000
136 T B 0.0000
137 R B 0.0000
138 G B 0.0000
139 Y B 0.0000
140 S B 0.0000
141 S B -0.1064
142 T B -0.0355
143 P B 0.0000
144 F B 0.0922
145 E B -0.8831
146 R B -1.6700
147 D B -0.6161
148 I B 0.0000
149 T B -0.3763
150 K B -1.7311
151 H B -0.5923
152 R B -1.0180
153 P B -0.2214
154 Q B 0.0000
155 V B 0.0000
156 Y B 0.0000
157 M B 0.0000
158 S B 0.0000
159 A B 0.0000
160 C B 0.0000
161 P B -0.0256
162 Y B 0.0000
163 Q B -0.1794
164 W B 0.2573
165 I B 0.6723
166 V B 0.0000
167 P B -0.1165
168 F B -0.1049
169 D B -1.9634
170 R B -1.7427
171 I B 0.0000
172 R B -2.0491
173 D B -2.2374
174 G B 0.0000
175 V B 0.0000
176 H B -0.5850
177 L B 0.0000
178 M B 0.0000
179 V B 0.0000
180 A B 0.0000
181 Q B -1.2367
182 S B -0.4291
183 V B 0.0000
184 R B -0.3392
185 R B 0.0000
186 T B 0.0000
187 P B -0.3692
188 R B -1.9256
189 S B -0.5441
190 S B 0.0000
191 I B 0.0000
192 D B -0.5240
193 P B 0.0000
194 Q B -0.5359
195 V B 0.0000
196 K B -0.3073
197 N B -0.2068
198 F B 0.0000
199 Q B 0.0000
200 W B 0.1377
201 G B -0.0292
202 D B 0.0000
203 L B 0.0000
204 I B 0.0000
205 R B -0.5421
206 A B 0.0000
207 I B 0.2911
208 Q B -0.1151
209 E B -0.2017
210 T B 0.0000
211 H B -1.1813
212 D B -2.0995
213 R B -1.2719
214 G B -0.5400
215 F B 0.2870
216 E B -0.3898
217 L B 0.0000
218 P B 0.0000
219 L B 0.0000
220 L B 0.0000
221 L B 0.0000
222 D B 0.0000
223 C B 0.4134
224 D B -1.8487
225 N B -1.3527
226 L B -0.0180
227 L B 0.0000
228 A B 0.0000
229 E B 0.0000
230 G B 0.0000
231 P B -0.0649
232 G B -0.1939
233 F B 0.0000
234 N B 0.0000
235 V B 0.0000
236 V B 0.0000
237 V B 0.0000
238 I B 0.0000
239 K B -0.8628
240 D B -1.9295
241 G B -0.4719
242 V B 0.4426
243 V B 0.0000
244 R B -0.2343
245 S B 0.0000
246 P B 0.0000
247 G B -0.4318
248 R B -1.8837
249 A B 0.0000
250 A B 0.0000
251 L B 0.1270
252 P B -0.0317
253 G B -0.1005
254 I B 0.0618
255 T B 0.0000
256 R B 0.0000
257 K B -1.1539
258 T B 0.0000
259 V B 0.0000
260 L B 0.0000
261 E B -1.8199
262 I B 0.0000
263 A B 0.0000
264 E B -1.8585
265 S B -0.4724
266 L B 0.2679
267 G B -0.4367
268 H B -0.6366
269 E B -1.8588
270 A B -0.1733
271 I B 1.0450
272 L B 1.2604
273 A B 0.0301
274 D B -1.0169
275 I B 0.0000
276 T B -0.0352
277 P B -0.0373
278 A B -0.0386
279 E B -0.4868
280 L B 0.0000
281 Y B 0.1754
282 D B -1.7003
283 A B 0.0000
284 D B -0.9137
285 E B 0.0000
286 V B 0.0000
287 L B 0.0000
288 G B 0.0000
289 C B 0.0000
290 S B -0.0298
291 T B -0.0341
292 G B -0.1180
293 G B 0.0000
294 G B 0.0000
295 V B 0.0000
296 W B 0.0000
297 P B 0.0000
298 F B 0.0000
299 V B 0.0000
300 S B 0.0000
301 V B 0.0000
302 D B -0.5507
303 G B -0.7053
304 N B -0.9838
305 S B -0.3697
306 I B 0.0000
307 S B -0.5318
308 D B -1.8512
309 G B -0.1350
310 V B 1.7434
311 P B 0.2761
312 G B -0.1181
313 P B -0.2353
314 V B 0.1294
315 T B 0.0000
316 Q B -1.2141
317 S B -0.3038
318 I B 0.0000
319 I B 0.0000
320 R B -1.2533
321 R B -1.1957
322 Y B 0.0000
323 W B 0.0000
324 E B -1.1725
325 L B 0.0727
326 N B 0.0000
327 V B 0.9662
328 E B -1.6040
329 P B -0.6025
330 S B -0.2354
331 S B -0.1854
332 L B 0.2769
333 L B 0.2274
334 T B -0.0388
335 P B -0.2876
336 V B 0.0000
337 Q B -0.9606
338 Y B 1.0996
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Automated mutations analysis - evolutionary conserved mutations

In the automated mutations mode, the server selects aggregation prone resides and each selected residue is mutated based off an evolutionary approach. The table below shows 2 best scored mutants for each mutated residue. Protein variants are ordered according to the mutation effect they had on protein stability (energetic effect) together with the difference in the average per-residue aggregation score between the wild type and the mutant (in the table green values indicate a positive change, grey are neutral, and orange/red mean destabilizing or more aggregation prone mutants).
Summary for all the mutants can be found in this CSV file .

Mutant
Energetic effect
Score comparison
VT19A -0.3112 -0.0037 View CSV PDB
VT19B -0.0102 -0.0037 View CSV PDB
IM105A -0.0532 -0.0015 View CSV PDB
VM310B -0.625 -0.0005 View CSV PDB
IL105A -0.063 -0.0004 View CSV PDB
VA19A 0.0126 -0.0034 View CSV PDB
IM105B 0.0326 -0.0015 View CSV PDB
VA19B 0.0932 -0.0042 View CSV PDB
VT310B 0.0884 -0.0038 View CSV PDB
VT310A 0.2329 -0.0038 View CSV PDB
IL105B 0.0367 -0.0004 View CSV PDB
VA310A 0.4585 -0.0036 View CSV PDB