| Chain sequence(s) |
A: DILPCVPFSVAKSVKSLYLGRMFSGTPVIRLRFKRLQPTRLVAEFDFRTFDPEGILLFAGGHQDSTWIVLALRAGRLELQLRYNGVGRVTSSGPVINHGMWQTISVEELARNLVIKVNRDAVMKIAVAGDLFQPERGLYHLNLTVGGIPFHEKDLVQPINPRLDGCMRSWNWLNGEDTTIQETVKVNTRMQCFSVTERGSFYPGSGFAFYSLDYMRTPLDVGTESTWEVEVVAHIRPAADTGVLFALWAPDLRAVPLSVALVDYHSTKKLKKQLVVLAVEHTALALMEIKVCDGQEHVVTVSLRDGEATLEVDGTRGQSEVSAAQLQERLAVLERHLRSPVLTFAGGLPDVPVTSAPVTAFYRGCMTLEVNRRLLDLDEAAYKHSDITAHSCPPVEPAAA
input PDB |
| Selected Chain(s) | A |
| Distance of aggregation | 10 Å |
| FoldX usage | Yes |
| pH calculations | No |
| alphaCutter usage | No |
| Dynamic mode | No |
| Automated mutations | Yes |
| Downloads | Download all the data |
| Simulation log |
[INFO] Logger: Verbosity set to: 2 - [INFO] (00:00:00)
[WARNING] runJob: Working directory already exists (possibly overwriting previous results -ow
to prevent this behavior) (00:00:00)
[INFO] runJob: Starting aggrescan3d job on: input.pdb with A chain(s) selected (00:00:00)
[INFO] runJob: Creating pdb object from: input.pdb (00:00:00)
[INFO] FoldX: Starting FoldX energy minimization (00:00:01)
[INFO] Analysis: Starting Aggrescan4D on folded.pdb (00:06:11)
[INFO] AutoMutEv:Residue number 5 from chain A and a score of 1.254 (cysteine) selected for
automated mutation (00:06:13)
[INFO] AutoMutEv:Residue number 6 from chain A and a score of 1.005 (valine) selected for
automated mutation (00:06:13)
[INFO] AutoMutEv:Residue number 89 from chain A and a score of 0.963 (valine) selected for
automated mutation (00:06:13)
[INFO] AutoMutEv:Residue number 209 from chain A and a score of 0.742 (phenylalanine)
selected for automated mutation (00:06:13)
[INFO] AutoMutEv:Residue number 342 from chain A and a score of 0.580 (leucine) selected for
automated mutation (00:06:13)
[INFO] AutoMutEv:Residue number 4 from chain A and a score of 0.577 (proline) selected for
automated mutation (00:06:13)
[INFO] AutoMutEv:Mutating residue number 5 from chain A (cysteine) into serine (00:06:13)
[INFO] AutoMutEv:Mutating residue number 89 from chain A (valine) into threonine (00:06:13)
[INFO] AutoMutEv:Mutating residue number 6 from chain A (valine) into alanine (00:06:13)
[INFO] AutoMutEv:Mutating residue number 6 from chain A (valine) into threonine (00:06:21)
[INFO] AutoMutEv:Mutating residue number 89 from chain A (valine) into alanine (00:06:22)
[INFO] AutoMutEv:Mutating residue number 6 from chain A (valine) into methionine (00:06:22)
[INFO] AutoMutEv:Mutating residue number 89 from chain A (valine) into methionine (00:06:30)
[INFO] AutoMutEv:Mutating residue number 209 from chain A (phenylalanine) into tryptophan (00:06:30)
[INFO] AutoMutEv:Mutating residue number 342 from chain A (leucine) into methionine (00:06:31)
[INFO] AutoMutEv:Mutating residue number 4 from chain A (proline) into arginine (00:06:44)
[INFO] AutoMutEv:Mutating residue number 209 from chain A (phenylalanine) into methionine (00:06:45)
[INFO] AutoMutEv:Mutating residue number 209 from chain A (phenylalanine) into tyrosine (00:06:48)
[INFO] AutoMutEv:Mutating residue number 4 from chain A (proline) into asparagine (00:06:57)
[INFO] AutoMutEv:Mutating residue number 4 from chain A (proline) into glutamine (00:07:15)
[INFO] AutoMutEv:Effect of mutation residue number 5 from chain A (cysteine) into serine:
Energy difference: -0.8134 kcal/mol, Difference in average score from the
base case: -0.0064 (00:07:35)
[INFO] AutoMutEv:Effect of mutation residue number 6 from chain A (valine) into threonine:
Energy difference: 0.7623 kcal/mol, Difference in average score from the
base case: -0.0039 (00:07:35)
[INFO] AutoMutEv:Effect of mutation residue number 6 from chain A (valine) into alanine:
Energy difference: 0.1507 kcal/mol, Difference in average score from the
base case: -0.0038 (00:07:35)
[INFO] AutoMutEv:Effect of mutation residue number 6 from chain A (valine) into methionine:
Energy difference: -0.3547 kcal/mol, Difference in average score from the
base case: 0.0007 (00:07:35)
[INFO] AutoMutEv:Effect of mutation residue number 89 from chain A (valine) into threonine:
Energy difference: 0.4760 kcal/mol, Difference in average score from the
base case: -0.0128 (00:07:35)
[INFO] AutoMutEv:Effect of mutation residue number 89 from chain A (valine) into alanine:
Energy difference: 0.7986 kcal/mol, Difference in average score from the
base case: -0.0154 (00:07:35)
[INFO] AutoMutEv:Effect of mutation residue number 89 from chain A (valine) into methionine:
Energy difference: -0.6532 kcal/mol, Difference in average score from the
base case: -0.0084 (00:07:35)
[INFO] AutoMutEv:Effect of mutation residue number 209 from chain A (phenylalanine) into
methionine: Energy difference: -1.1688 kcal/mol, Difference in average
score from the base case: -0.0070 (00:07:35)
[INFO] AutoMutEv:Effect of mutation residue number 209 from chain A (phenylalanine) into
tryptophan: Energy difference: -0.5002 kcal/mol, Difference in average
score from the base case: -0.0053 (00:07:35)
[INFO] AutoMutEv:Effect of mutation residue number 209 from chain A (phenylalanine) into
tyrosine: Energy difference: 0.5468 kcal/mol, Difference in average score
from the base case: -0.0009 (00:07:35)
[INFO] AutoMutEv:Effect of mutation residue number 342 from chain A (leucine) into
methionine: Energy difference: -0.0627 kcal/mol, Difference in average
score from the base case: -0.0045 (00:07:35)
[INFO] AutoMutEv:Effect of mutation residue number 4 from chain A (proline) into arginine:
Energy difference: 2.6163 kcal/mol, Difference in average score from the
base case: -0.0126 (00:07:35)
[INFO] AutoMutEv:Effect of mutation residue number 4 from chain A (proline) into asparagine:
Energy difference: 1.2708 kcal/mol, Difference in average score from the
base case: -0.0080 (00:07:35)
[INFO] AutoMutEv:Effect of mutation residue number 4 from chain A (proline) into glutamine:
Energy difference: 2.3005 kcal/mol, Difference in average score from the
base case: -0.0077 (00:07:35)
[INFO] Main: Simulation completed successfully. (00:07:43)
|
The table below lists A4D score for protein residues. Residues with A4D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan4D score | mutation |
|---|---|---|---|---|
| 1 | D | A | -1.4556 | |
| 2 | I | A | 0.0000 | |
| 3 | L | A | 0.5586 | |
| 4 | P | A | 0.5770 | |
| 5 | C | A | 1.2538 | |
| 6 | V | A | 1.0052 | |
| 7 | P | A | 0.3140 | |
| 8 | F | A | 0.2979 | |
| 9 | S | A | -0.0623 | |
| 10 | V | A | 0.3584 | |
| 11 | A | A | -0.2053 | |
| 12 | K | A | -1.3970 | |
| 13 | S | A | -1.1508 | |
| 14 | V | A | -0.5937 | |
| 15 | K | A | -1.9780 | |
| 16 | S | A | 0.0000 | |
| 17 | L | A | 0.0000 | |
| 18 | Y | A | 0.0000 | |
| 19 | L | A | 0.0000 | |
| 20 | G | A | 0.0000 | |
| 21 | R | A | -1.8864 | |
| 22 | M | A | -0.7140 | |
| 23 | F | A | 0.0000 | |
| 24 | S | A | -0.6933 | |
| 25 | G | A | -0.6041 | |
| 26 | T | A | 0.0000 | |
| 27 | P | A | 0.0000 | |
| 28 | V | A | 0.0000 | |
| 29 | I | A | -0.2681 | |
| 30 | R | A | -1.8747 | |
| 31 | L | A | -1.6435 | |
| 32 | R | A | -2.5889 | |
| 33 | F | A | -1.4430 | |
| 34 | K | A | -1.7504 | |
| 35 | R | A | -0.8691 | |
| 36 | L | A | 0.1172 | |
| 37 | Q | A | -0.8478 | |
| 38 | P | A | -0.6935 | |
| 39 | T | A | -0.5150 | |
| 40 | R | A | -0.8013 | |
| 41 | L | A | 0.0000 | |
| 42 | V | A | -0.0290 | |
| 43 | A | A | 0.0000 | |
| 44 | E | A | -1.6325 | |
| 45 | F | A | 0.0000 | |
| 46 | D | A | 0.0000 | |
| 47 | F | A | 0.0000 | |
| 48 | R | A | -1.1192 | |
| 49 | T | A | 0.0000 | |
| 50 | F | A | 0.0000 | |
| 51 | D | A | -2.7045 | |
| 52 | P | A | 0.0000 | |
| 53 | E | A | -1.2671 | |
| 54 | G | A | 0.0000 | |
| 55 | I | A | 0.0000 | |
| 56 | L | A | 0.0000 | |
| 57 | L | A | 0.0000 | |
| 58 | F | A | 0.0000 | |
| 59 | A | A | 0.0000 | |
| 60 | G | A | 0.0000 | |
| 61 | G | A | 0.0000 | |
| 62 | H | A | -2.4464 | |
| 63 | Q | A | -2.7164 | |
| 64 | D | A | -2.8718 | |
| 65 | S | A | -2.2877 | |
| 66 | T | A | 0.0000 | |
| 67 | W | A | 0.0000 | |
| 68 | I | A | 0.0000 | |
| 69 | V | A | 0.0000 | |
| 70 | L | A | 0.0000 | |
| 71 | A | A | 0.0000 | |
| 72 | L | A | 0.0000 | |
| 73 | R | A | -1.9104 | |
| 74 | A | A | -1.8269 | |
| 75 | G | A | -1.8931 | |
| 76 | R | A | -2.4304 | |
| 77 | L | A | 0.0000 | |
| 78 | E | A | -0.7340 | |
| 79 | L | A | 0.0000 | |
| 80 | Q | A | 0.0000 | |
| 81 | L | A | 0.0000 | |
| 82 | R | A | -1.1740 | |
| 83 | Y | A | -1.3601 | |
| 84 | N | A | -2.0986 | |
| 85 | G | A | -1.1476 | |
| 86 | V | A | -0.6333 | |
| 87 | G | A | -0.6099 | |
| 88 | R | A | -0.1620 | |
| 89 | V | A | 0.9631 | |
| 90 | T | A | 0.0000 | |
| 91 | S | A | -0.4599 | |
| 92 | S | A | 0.0000 | |
| 93 | G | A | -1.2152 | |
| 94 | P | A | -1.1257 | |
| 95 | V | A | 0.0000 | |
| 96 | I | A | 0.0000 | |
| 97 | N | A | -1.6353 | |
| 98 | H | A | -1.7413 | |
| 99 | G | A | -0.8969 | |
| 100 | M | A | 0.1227 | |
| 101 | W | A | 0.1348 | |
| 102 | Q | A | 0.0000 | |
| 103 | T | A | -1.4067 | |
| 104 | I | A | 0.0000 | |
| 105 | S | A | -1.3513 | |
| 106 | V | A | 0.0000 | |
| 107 | E | A | -1.3631 | |
| 108 | E | A | 0.0000 | |
| 109 | L | A | 0.1726 | |
| 110 | A | A | -0.6569 | |
| 111 | R | A | -1.9158 | |
| 112 | N | A | -1.3367 | |
| 113 | L | A | 0.0000 | |
| 114 | V | A | 0.0000 | |
| 115 | I | A | 0.0000 | |
| 116 | K | A | -2.9534 | |
| 117 | V | A | 0.0000 | |
| 118 | N | A | -2.1836 | |
| 119 | R | A | -3.1959 | |
| 120 | D | A | -3.0339 | |
| 121 | A | A | -2.0202 | |
| 122 | V | A | -1.1894 | |
| 123 | M | A | 0.0000 | |
| 124 | K | A | -1.8488 | |
| 125 | I | A | -0.7530 | |
| 126 | A | A | -0.7968 | |
| 127 | V | A | 0.0000 | |
| 128 | A | A | -0.8315 | |
| 129 | G | A | -0.9701 | |
| 130 | D | A | -1.3888 | |
| 131 | L | A | 0.0000 | |
| 132 | F | A | 0.0000 | |
| 133 | Q | A | -1.5473 | |
| 134 | P | A | -1.5411 | |
| 135 | E | A | -2.1174 | |
| 136 | R | A | -2.2711 | |
| 137 | G | A | -1.3453 | |
| 138 | L | A | -1.0534 | |
| 139 | Y | A | -1.0033 | |
| 140 | H | A | -1.5241 | |
| 141 | L | A | 0.0000 | |
| 142 | N | A | -1.8294 | |
| 143 | L | A | 0.0000 | |
| 144 | T | A | 0.0000 | |
| 145 | V | A | 0.0000 | |
| 146 | G | A | 0.0000 | |
| 147 | G | A | 0.0000 | |
| 148 | I | A | -0.1152 | |
| 149 | P | A | -0.7330 | |
| 150 | F | A | -0.5885 | |
| 151 | H | A | -1.7591 | |
| 152 | E | A | -2.5641 | |
| 153 | K | A | -2.1367 | |
| 154 | D | A | -2.5700 | |
| 155 | L | A | 0.0000 | |
| 156 | V | A | -0.6944 | |
| 157 | Q | A | -0.8014 | |
| 158 | P | A | -0.8358 | |
| 159 | I | A | -0.4272 | |
| 160 | N | A | -0.4751 | |
| 161 | P | A | 0.0000 | |
| 162 | R | A | -1.0965 | |
| 163 | L | A | 0.0000 | |
| 164 | D | A | 0.0000 | |
| 165 | G | A | 0.0000 | |
| 166 | C | A | 0.0000 | |
| 167 | M | A | 0.0000 | |
| 168 | R | A | -2.4406 | |
| 169 | S | A | -1.7096 | |
| 170 | W | A | 0.0000 | |
| 171 | N | A | -1.5453 | |
| 172 | W | A | 0.0000 | |
| 173 | L | A | 0.0000 | |
| 174 | N | A | -1.3334 | |
| 175 | G | A | -1.3278 | |
| 176 | E | A | -2.3102 | |
| 177 | D | A | 0.0000 | |
| 178 | T | A | -1.3138 | |
| 179 | T | A | -1.1605 | |
| 180 | I | A | 0.0000 | |
| 181 | Q | A | -1.6794 | |
| 182 | E | A | -1.7311 | |
| 183 | T | A | -0.6460 | |
| 184 | V | A | 0.0000 | |
| 185 | K | A | -0.9428 | |
| 186 | V | A | 0.5038 | |
| 187 | N | A | -0.6240 | |
| 188 | T | A | -0.7406 | |
| 189 | R | A | -1.9258 | |
| 190 | M | A | -1.3147 | |
| 191 | Q | A | -1.0998 | |
| 192 | C | A | 0.0000 | |
| 193 | F | A | 0.0000 | |
| 194 | S | A | -0.6159 | |
| 195 | V | A | 0.1199 | |
| 196 | T | A | -0.9081 | |
| 197 | E | A | -2.2690 | |
| 198 | R | A | -2.5055 | |
| 199 | G | A | -1.4845 | |
| 200 | S | A | 0.0000 | |
| 201 | F | A | 0.0000 | |
| 202 | Y | A | 0.0000 | |
| 203 | P | A | -0.5206 | |
| 204 | G | A | -0.5570 | |
| 205 | S | A | -0.5343 | |
| 206 | G | A | -0.6991 | |
| 207 | F | A | -0.3552 | |
| 208 | A | A | 0.0000 | |
| 209 | F | A | 0.7416 | |
| 210 | Y | A | 0.5026 | |
| 211 | S | A | -0.0759 | |
| 212 | L | A | 0.0000 | |
| 213 | D | A | -2.1065 | |
| 214 | Y | A | -1.6078 | |
| 215 | M | A | -1.4801 | |
| 216 | R | A | -2.1054 | |
| 217 | T | A | -1.0818 | |
| 218 | P | A | -0.9487 | |
| 219 | L | A | -0.2581 | |
| 220 | D | A | -1.4383 | |
| 221 | V | A | -0.5354 | |
| 222 | G | A | -1.3588 | |
| 223 | T | A | -1.6630 | |
| 224 | E | A | -2.0866 | |
| 225 | S | A | -2.0065 | |
| 226 | T | A | -1.7933 | |
| 227 | W | A | 0.0000 | |
| 228 | E | A | -2.7611 | |
| 229 | V | A | 0.0000 | |
| 230 | E | A | -2.3234 | |
| 231 | V | A | 0.0000 | |
| 232 | V | A | -0.5609 | |
| 233 | A | A | 0.0000 | |
| 234 | H | A | -0.8785 | |
| 235 | I | A | 0.0000 | |
| 236 | R | A | -1.9610 | |
| 237 | P | A | 0.0000 | |
| 238 | A | A | 0.0000 | |
| 239 | A | A | 0.0000 | |
| 240 | D | A | 0.0000 | |
| 241 | T | A | 0.0000 | |
| 242 | G | A | 0.0000 | |
| 243 | V | A | 0.0000 | |
| 244 | L | A | 0.0000 | |
| 245 | F | A | 0.0000 | |
| 246 | A | A | 0.0000 | |
| 247 | L | A | 0.0000 | |
| 248 | W | A | 0.0271 | |
| 249 | A | A | 0.0000 | |
| 250 | P | A | -0.8697 | |
| 251 | D | A | -1.6326 | |
| 252 | L | A | -0.0112 | |
| 253 | R | A | -0.6142 | |
| 254 | A | A | -0.3178 | |
| 255 | V | A | 0.0000 | |
| 256 | P | A | 0.0000 | |
| 257 | L | A | 0.0000 | |
| 258 | S | A | 0.0000 | |
| 259 | V | A | 0.0000 | |
| 260 | A | A | 0.0000 | |
| 261 | L | A | 0.0000 | |
| 262 | V | A | -0.1116 | |
| 263 | D | A | 0.0000 | |
| 264 | Y | A | -0.6049 | |
| 265 | H | A | -0.8930 | |
| 266 | S | A | -1.1527 | |
| 267 | T | A | -1.1216 | |
| 268 | K | A | -1.9836 | |
| 269 | K | A | -1.7294 | |
| 270 | L | A | -0.9009 | |
| 271 | K | A | -1.8539 | |
| 272 | K | A | -2.1775 | |
| 273 | Q | A | 0.0000 | |
| 274 | L | A | -0.8904 | |
| 275 | V | A | 0.0000 | |
| 276 | V | A | 0.0000 | |
| 277 | L | A | 0.0000 | |
| 278 | A | A | 0.0000 | |
| 279 | V | A | 0.0000 | |
| 280 | E | A | 0.0000 | |
| 281 | H | A | -0.8348 | |
| 282 | T | A | -0.6035 | |
| 283 | A | A | 0.0000 | |
| 284 | L | A | 0.0000 | |
| 285 | A | A | 0.0000 | |
| 286 | L | A | -1.4873 | |
| 287 | M | A | 0.0000 | |
| 288 | E | A | -3.0541 | |
| 289 | I | A | 0.0000 | |
| 290 | K | A | -3.5529 | |
| 291 | V | A | 0.0000 | |
| 292 | C | A | 0.0000 | |
| 293 | D | A | -3.1693 | |
| 294 | G | A | -2.2883 | |
| 295 | Q | A | -2.1578 | |
| 296 | E | A | -2.6527 | |
| 297 | H | A | 0.0000 | |
| 298 | V | A | -0.6212 | |
| 299 | V | A | 0.0000 | |
| 300 | T | A | -0.9462 | |
| 301 | V | A | 0.0000 | |
| 302 | S | A | 0.0000 | |
| 303 | L | A | 0.0000 | |
| 304 | R | A | -2.7774 | |
| 305 | D | A | -2.8441 | |
| 306 | G | A | -2.5516 | |
| 307 | E | A | -2.6117 | |
| 308 | A | A | 0.0000 | |
| 309 | T | A | -1.3972 | |
| 310 | L | A | 0.0000 | |
| 311 | E | A | -2.3490 | |
| 312 | V | A | 0.0000 | |
| 313 | D | A | -3.1841 | |
| 314 | G | A | -2.4576 | |
| 315 | T | A | -2.9471 | |
| 316 | R | A | -3.3783 | |
| 317 | G | A | -2.4046 | |
| 318 | Q | A | -2.3702 | |
| 319 | S | A | -1.8035 | |
| 320 | E | A | -2.1216 | |
| 321 | V | A | -1.4769 | |
| 322 | S | A | -0.8101 | |
| 323 | A | A | -1.1122 | |
| 324 | A | A | -1.1461 | |
| 325 | Q | A | -1.9071 | |
| 326 | L | A | -1.8950 | |
| 327 | Q | A | -2.6890 | |
| 328 | E | A | -2.6052 | |
| 329 | R | A | 0.0000 | |
| 330 | L | A | 0.0000 | |
| 331 | A | A | -2.0254 | |
| 332 | V | A | 0.0000 | |
| 333 | L | A | 0.0000 | |
| 334 | E | A | -1.9421 | |
| 335 | R | A | -2.6752 | |
| 336 | H | A | 0.0000 | |
| 337 | L | A | 0.0000 | |
| 338 | R | A | -1.8844 | |
| 339 | S | A | 0.0000 | |
| 340 | P | A | -0.7157 | |
| 341 | V | A | 0.0000 | |
| 342 | L | A | 0.5795 | |
| 343 | T | A | 0.0000 | |
| 344 | F | A | 0.0000 | |
| 345 | A | A | 0.0000 | |
| 346 | G | A | 0.0000 | |
| 347 | G | A | 0.0000 | |
| 348 | L | A | 0.0000 | |
| 349 | P | A | -0.9768 | |
| 350 | D | A | -1.7701 | |
| 351 | V | A | 0.0000 | |
| 352 | P | A | -0.3882 | |
| 353 | V | A | 0.3483 | |
| 354 | T | A | 0.0882 | |
| 355 | S | A | -0.0471 | |
| 356 | A | A | 0.0000 | |
| 357 | P | A | 0.0997 | |
| 358 | V | A | 0.2839 | |
| 359 | T | A | -0.0087 | |
| 360 | A | A | 0.0087 | |
| 361 | F | A | 0.0366 | |
| 362 | Y | A | 0.0000 | |
| 363 | R | A | -0.6701 | |
| 364 | G | A | 0.0000 | |
| 365 | C | A | 0.0000 | |
| 366 | M | A | 0.0000 | |
| 367 | T | A | -0.6422 | |
| 368 | L | A | 0.0000 | |
| 369 | E | A | -1.7006 | |
| 370 | V | A | 0.0000 | |
| 371 | N | A | -3.1124 | |
| 372 | R | A | -3.1649 | |
| 373 | R | A | -2.4066 | |
| 374 | L | A | -0.2737 | |
| 375 | L | A | 0.0000 | |
| 376 | D | A | -0.6063 | |
| 377 | L | A | 0.0000 | |
| 378 | D | A | 0.0000 | |
| 379 | E | A | -1.2973 | |
| 380 | A | A | -0.3052 | |
| 381 | A | A | -0.3626 | |
| 382 | Y | A | 0.0000 | |
| 383 | K | A | -0.9878 | |
| 384 | H | A | -0.8577 | |
| 385 | S | A | -1.0406 | |
| 386 | D | A | -1.2609 | |
| 387 | I | A | 0.0000 | |
| 388 | T | A | 0.0000 | |
| 389 | A | A | 0.0000 | |
| 390 | H | A | -1.6159 | |
| 391 | S | A | 0.0000 | |
| 392 | C | A | 0.0000 | |
| 393 | P | A | 0.0000 | |
| 394 | P | A | -0.5624 | |
| 395 | V | A | 0.0527 | |
| 396 | E | A | -1.3938 | |
| 397 | P | A | -0.8176 | |
| 398 | A | A | -0.5657 | |
| 399 | A | A | -0.9135 | |
| 400 | A | A | -0.5595 |
Automated mutations analysis - evolutionary conserved mutations
In the automated mutations mode, the server selects aggregation prone resides
and each selected residue is mutated based off an evolutionary approach.
The table below shows 2 best scored mutants for each mutated residue. Protein variants
are ordered according to the mutation effect they had on protein stability
(energetic effect) together with the difference in the average per-residue aggregation score
between the wild type and the mutant (in the table green values indicate a positive change,
grey are neutral, and orange/red mean destabilizing or more aggregation prone mutants).
Summary for all the mutants can be found in this
CSV file .
Mutant |
Energetic effect |
Score comparison |
|||
| FM209A | -1.1688 | -0.007 | View | CSV | PDB |
| VM89A | -0.6532 | -0.0084 | View | CSV | PDB |
| CS5A | -0.8134 | -0.0064 | View | CSV | PDB |
| FW209A | -0.5002 | -0.0053 | View | CSV | PDB |
| LM342A | -0.0627 | -0.0045 | View | CSV | PDB |
| VT89A | 0.476 | -0.0128 | View | CSV | PDB |
| VA6A | 0.1507 | -0.0038 | View | CSV | PDB |
| PN4A | 1.2708 | -0.008 | View | CSV | PDB |
| VT6A | 0.7623 | -0.0039 | View | CSV | PDB |
| PR4A | 2.6163 | -0.0126 | View | CSV | PDB |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine