Aggrescan4D: F2B_K0

Project name: F2B_K0_H0

Status: done

Started: 2026-05-29 06:46:44

Chain sequence(s)	A: EVQLVESGGGLVKPGGSLKLSCAASGYTFTSYVMHWVRQAPGKGLEWIGYINPYNDGTKYNEKFQGRVTISSDKSISTAYMELSSLRSEDTAMYYCARGTYYYGTRVFDYWGQGTLVTVSSGGGGSGGGGSGGGGSDIVMTQSPATLSLSPGERATLSCRSSKSLQNVNGNTYLYWFQQKPGQSPQLLIYRMSNLNSGVPDRFSGSGSGTEFTLTISSLEPEDFAVYYCMQHLEYPITFGAGTKLEIKSGGGGSEVQLVESGGGLVQPGRSLRLSCAASGFTFDDYAMHWVRQAPGKGLEWVSGISWNSGSIGYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTALYYCAKDSRGYGDYRLGGAYWGQGTLVTVSSGGGGSGGGGSGGGGSEIVMTQSPATLSVSPGERATLSCRASQSVSSNLAWYQQKPGQAPRLLIYGASTRATGIPARFSGSGSGTEFTLTISSLQSEDFAVYYCQQYNNWPWTFGQGTKVEIKGGGGDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLYITREPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALAAPIEKTISKAKGQPREPQVYTLPPCREEMTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK B: DVQLVESGGGLVQAGGSLRLSCRASGHTFSDTAVGWFRRVPGKEREFVATIAGSGSVRYSESVKGRFTISGDNAKNMVYLQMNRLKPEDTGVYYCAAAVGISYDYWGQGTQVTVSSEPKSSDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLYITREPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALAAPIEKTISKAKGQPREPQVCTLPPSREEMTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK input PDB
Selected Chain(s)	A,B
Distance of aggregation	10 Å
FoldX usage	Yes
pH calculations	No
alphaCutter usage	No
Dynamic mode	No
Automated mutations	Yes
Downloads	Download all the data

Simulation log

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[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimization                                          (00:00:01)
[INFO]       Analysis: Starting Aggrescan4D on folded.pdb                                          (00:13:14)
[INFO]       AutoMutEv:Residue number 101 from chain B and a score of 1.808 (isoleucine) selected  
                       for automated mutation                                                      (00:13:23)
[INFO]       AutoMutEv:Residue number 536 from chain A and a score of 1.370 (isoleucine) selected  
                       for automated mutation                                                      (00:13:23)
[INFO]       AutoMutEv:Residue number 76 from chain A and a score of 1.353 (isoleucine) selected   
                       for automated mutation                                                      (00:13:23)
[INFO]       AutoMutEv:Residue number 11 from chain A and a score of 1.235 (leucine) selected for  
                       automated mutation                                                          (00:13:23)
[INFO]       AutoMutEv:Residue number 154 from chain B and a score of 1.186 (isoleucine) selected  
                       for automated mutation                                                      (00:13:23)
[INFO]       AutoMutEv:Residue number 116 from chain A and a score of 1.095 (leucine) selected for 
                       automated mutation                                                          (00:13:23)
[INFO]       AutoMutEv:Mutating residue number 101 from chain B (isoleucine) into threonine        (00:13:23)
[INFO]       AutoMutEv:Mutating residue number 101 from chain B (isoleucine) into leucine          (00:13:23)
[INFO]       AutoMutEv:Mutating residue number 536 from chain A (isoleucine) into methionine       (00:13:23)
[INFO]       AutoMutEv:Mutating residue number 101 from chain B (isoleucine) into methionine       (00:13:45)
[INFO]       AutoMutEv:Mutating residue number 536 from chain A (isoleucine) into threonine        (00:13:45)
[INFO]       AutoMutEv:Mutating residue number 536 from chain A (isoleucine) into leucine          (00:13:46)
[INFO]       AutoMutEv:Mutating residue number 76 from chain A (isoleucine) into threonine         (00:14:08)
[INFO]       AutoMutEv:Mutating residue number 76 from chain A (isoleucine) into leucine           (00:14:08)
[INFO]       AutoMutEv:Mutating residue number 154 from chain B (isoleucine) into threonine        (00:14:12)
[INFO]       AutoMutEv:Mutating residue number 76 from chain A (isoleucine) into methionine        (00:14:31)
[INFO]       AutoMutEv:Mutating residue number 11 from chain A (leucine) into methionine           (00:14:32)
[INFO]       AutoMutEv:Mutating residue number 154 from chain B (isoleucine) into methionine       (00:14:35)
[INFO]       AutoMutEv:Mutating residue number 154 from chain B (isoleucine) into leucine          (00:14:54)
[INFO]       AutoMutEv:Mutating residue number 116 from chain A (leucine) into methionine          (00:15:17)
[INFO]       AutoMutEv:Effect of mutation residue number 101 from chain B (isoleucine) into        
                       threonine: Energy difference: 0.9879 kcal/mol, Difference in average score  
                       from the base case: -0.0057                                                 (00:15:39)
[INFO]       AutoMutEv:Effect of mutation residue number 101 from chain B (isoleucine) into        
                       methionine: Energy difference: 0.0742 kcal/mol, Difference in average score 
                       from the base case: -0.0034                                                 (00:15:39)
[INFO]       AutoMutEv:Effect of mutation residue number 101 from chain B (isoleucine) into        
                       leucine: Energy difference: 0.7739 kcal/mol, Difference in average score    
                       from the base case: -0.0007                                                 (00:15:39)
[INFO]       AutoMutEv:Effect of mutation residue number 536 from chain A (isoleucine) into        
                       threonine: Energy difference: 0.3543 kcal/mol, Difference in average score  
                       from the base case: -0.0060                                                 (00:15:39)
[INFO]       AutoMutEv:Effect of mutation residue number 536 from chain A (isoleucine) into        
                       methionine: Energy difference: -0.5463 kcal/mol, Difference in average      
                       score from the base case: -0.0011                                           (00:15:39)
[INFO]       AutoMutEv:Effect of mutation residue number 536 from chain A (isoleucine) into        
                       leucine: Energy difference: -0.5040 kcal/mol, Difference in average score   
                       from the base case: -0.0004                                                 (00:15:39)
[INFO]       AutoMutEv:Effect of mutation residue number 76 from chain A (isoleucine) into         
                       threonine: Energy difference: 0.7805 kcal/mol, Difference in average score  
                       from the base case: -0.0058                                                 (00:15:39)
[INFO]       AutoMutEv:Effect of mutation residue number 76 from chain A (isoleucine) into         
                       methionine: Energy difference: 0.3543 kcal/mol, Difference in average score 
                       from the base case: -0.0025                                                 (00:15:39)
[INFO]       AutoMutEv:Effect of mutation residue number 76 from chain A (isoleucine) into         
                       leucine: Energy difference: 0.5073 kcal/mol, Difference in average score    
                       from the base case: -0.0007                                                 (00:15:39)
[INFO]       AutoMutEv:Effect of mutation residue number 11 from chain A (leucine) into            
                       methionine: Energy difference: -0.0365 kcal/mol, Difference in average      
                       score from the base case: -0.0015                                           (00:15:39)
[INFO]       AutoMutEv:Effect of mutation residue number 154 from chain B (isoleucine) into        
                       threonine: Energy difference: 0.3192 kcal/mol, Difference in average score  
                       from the base case: -0.0069                                                 (00:15:39)
[INFO]       AutoMutEv:Effect of mutation residue number 154 from chain B (isoleucine) into        
                       methionine: Energy difference: -0.5711 kcal/mol, Difference in average      
                       score from the base case: -0.0019                                           (00:15:39)
[INFO]       AutoMutEv:Effect of mutation residue number 154 from chain B (isoleucine) into        
                       leucine: Energy difference: -0.5530 kcal/mol, Difference in average score   
                       from the base case: -0.0009                                                 (00:15:39)
[INFO]       AutoMutEv:Effect of mutation residue number 116 from chain A (leucine) into           
                       methionine: Energy difference: -0.2269 kcal/mol, Difference in average      
                       score from the base case: -0.0025                                           (00:15:39)
[INFO]       Main:     Simulation completed successfully.                                          (00:15:47)

Note Your prediction results will be deleted after 360 days.

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Show buried residues

Minimal score value: -3.1499
Maximal score value: 1.8075
Average score: -0.7154
Total score value: -771.1533

Download the table (csv)

The table below lists A4D score for protein residues. Residues with A4D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan4D score

1	E	A	-1.9717
2	V	A	0.0000
3	Q	A	-1.5169
4	L	A	0.0000
5	V	A	0.6402
6	E	A	0.0000
7	S	A	-0.2954
8	G	A	-0.6650
9	G	A	-0.0175
10	G	A	0.7269
11	L	A	1.2349
12	V	A	-0.3317
13	K	A	-1.9360
14	P	A	-1.9624
15	G	A	-1.4783
16	G	A	-1.1611
17	S	A	-1.2496
18	L	A	-1.0694
19	K	A	-2.1220
20	L	A	0.0000
21	S	A	-0.4415
22	C	A	0.0000
23	A	A	0.5016
24	A	A	0.0000
25	S	A	-0.5872
26	G	A	-0.8660
27	Y	A	-0.1781
28	T	A	-0.1145
29	F	A	0.0000
30	T	A	-0.2540
31	S	A	0.0000
32	Y	A	0.0000
33	V	A	0.0000
34	M	A	0.0000
35	H	A	0.0000
36	W	A	0.0000
37	V	A	0.0000
38	R	A	0.0000
39	Q	A	-0.6543
40	A	A	-1.0640
41	P	A	-0.9016
42	G	A	-1.4536
43	K	A	-2.1359
44	G	A	-1.1317
45	L	A	0.0000
46	E	A	-0.5955
47	W	A	0.0000
48	I	A	0.0000
49	G	A	0.0000
50	Y	A	0.0000
51	I	A	0.0000
52	N	A	0.0000
53	P	A	0.0000
54	Y	A	-0.4135
55	N	A	-1.3419
56	D	A	-2.0028
57	G	A	-1.1308
58	T	A	-0.7961
59	K	A	-0.5877
60	Y	A	-0.9664
61	N	A	-1.5828
62	E	A	-3.1056
63	K	A	-3.0950
64	F	A	0.0000
65	Q	A	-2.7224
66	G	A	-1.6993
67	R	A	-1.5748
68	V	A	0.0000
69	T	A	-0.9007
70	I	A	0.0000
71	S	A	-0.7811
72	S	A	-0.7177
73	D	A	-0.8440
74	K	A	-0.8846
75	S	A	0.0517
76	I	A	1.3525
77	S	A	0.2574
78	T	A	0.0000
79	A	A	0.0000
80	Y	A	-0.6411
81	M	A	0.0000
82	E	A	-1.6294
83	L	A	0.0000
84	S	A	-1.1358
85	S	A	-1.1807
86	L	A	0.0000
87	R	A	-2.6576
88	S	A	-2.0816
89	E	A	-2.2144
90	D	A	0.0000
91	T	A	-0.5330
92	A	A	0.0000
93	M	A	0.4469
94	Y	A	0.0000
95	Y	A	0.0000
96	C	A	0.0000
97	A	A	0.0000
98	R	A	0.0000
99	G	A	0.0000
100	T	A	0.0000
101	Y	A	0.0000
102	Y	A	0.0000
103	Y	A	-0.0586
104	G	A	0.0000
105	T	A	-0.4551
106	R	A	-0.6870
107	V	A	0.0000
108	F	A	0.0000
109	D	A	0.0000
110	Y	A	-0.2294
111	W	A	0.0000
112	G	A	0.0000
113	Q	A	-1.1745
114	G	A	0.0000
115	T	A	0.3188
116	L	A	1.0952
117	V	A	0.0000
118	T	A	0.1092
119	V	A	0.0000
120	S	A	-1.0674
121	S	A	-1.6082
122	G	A	-1.3422
123	G	A	-1.1118
124	G	A	-1.2044
125	G	A	-1.1420
126	S	A	-0.9968
127	G	A	-1.1689
128	G	A	-1.1924
129	G	A	-1.1834
130	G	A	-1.1855
131	S	A	-1.0137
132	G	A	-1.3706
133	G	A	-1.1724
134	G	A	-1.2702
135	G	A	-1.4933
136	S	A	-1.1697
137	D	A	-1.2903
138	I	A	0.0000
139	V	A	0.7665
140	M	A	0.0000
141	T	A	-0.4756
142	Q	A	0.0000
143	S	A	-0.5522
144	P	A	-0.2819
145	A	A	-0.1993
146	T	A	-0.4783
147	L	A	-0.0838
148	S	A	-0.1203
149	L	A	-0.3036
150	S	A	0.0000
151	P	A	-0.8041
152	G	A	0.0000
153	E	A	-1.7689
154	R	A	-2.3169
155	A	A	0.0000
156	T	A	-0.5844
157	L	A	0.0000
158	S	A	-0.9882
159	C	A	0.0000
160	R	A	-2.6373
161	S	A	0.0000
162	S	A	-1.2521
163	K	A	-2.2596
164	S	A	-1.8248
165	L	A	0.0000
166	Q	A	-0.9114
167	N	A	0.0777
168	V	A	0.9782
169	N	A	0.0000
170	G	A	-0.5419
171	N	A	-0.4605
172	T	A	0.0000
173	Y	A	0.0000
174	L	A	0.0000
175	Y	A	0.0000
176	W	A	0.0000
177	F	A	0.0000
178	Q	A	-0.6925
179	Q	A	0.0000
180	K	A	-1.4480
181	P	A	-0.9838
182	G	A	-1.3703
183	Q	A	-1.9401
184	S	A	-1.3246
185	P	A	0.0000
186	Q	A	-0.9882
187	L	A	0.0000
188	L	A	0.0000
189	I	A	0.0000
190	Y	A	-0.4308
191	R	A	-0.8386
192	M	A	-0.5754
193	S	A	-0.6474
194	N	A	-0.8810
195	L	A	-0.4288
196	N	A	0.0000
197	S	A	-0.4679
198	G	A	-0.6251
199	V	A	-0.5345
200	P	A	-1.0029
201	D	A	-1.8975
202	R	A	-1.4073
203	F	A	0.0000
204	S	A	-0.6985
205	G	A	0.0000
206	S	A	-0.8624
207	G	A	-1.2859
208	S	A	-1.2816
209	G	A	-1.5890
210	T	A	-2.2685
211	E	A	-2.9250
212	F	A	0.0000
213	T	A	-0.9087
214	L	A	0.0000
215	T	A	-0.5891
216	I	A	0.0000
217	S	A	-1.4058
218	S	A	-1.3738
219	L	A	0.0000
220	E	A	-1.5991
221	P	A	-1.2472
222	E	A	-2.1988
223	D	A	0.0000
224	F	A	0.0000
225	A	A	0.0000
226	V	A	-0.4081
227	Y	A	0.0000
228	Y	A	0.0000
229	C	A	0.0000
230	M	A	0.0000
231	Q	A	0.0000
232	H	A	0.0000
233	L	A	-0.5382
234	E	A	-0.8156
235	Y	A	0.1524
236	P	A	-0.5173
237	I	A	0.0000
238	T	A	0.2150
239	F	A	0.3953
240	G	A	0.0000
241	A	A	-0.1905
242	G	A	0.0000
243	T	A	0.0000
244	K	A	-0.8042
245	L	A	0.0000
246	E	A	-0.3768
247	I	A	1.0105
248	K	A	-0.0866
249	S	A	-0.3653
250	G	A	-0.8029
251	G	A	-1.0224
252	G	A	-1.0559
253	G	A	-1.0546
254	S	A	-1.2546
255	E	A	-1.6557
256	V	A	-0.5882
257	Q	A	-0.5167
258	L	A	0.0000
259	V	A	0.3123
260	E	A	0.0000
261	S	A	-0.6454
262	G	A	-0.9742
263	G	A	-0.7192
264	G	A	-0.4357
265	L	A	0.1498
266	V	A	-0.7671
267	Q	A	-1.6943
268	P	A	-2.0716
269	G	A	-2.1013
270	R	A	-2.6810
271	S	A	-1.9364
272	L	A	-1.5800
273	R	A	-2.1618
274	L	A	0.0000
275	S	A	-0.5999
276	C	A	0.0000
277	A	A	-0.2803
278	A	A	0.0000
279	S	A	-0.5129
280	G	A	0.0000
281	F	A	-0.4746
282	T	A	-0.5294
283	F	A	0.0000
284	D	A	-1.5195
285	D	A	-0.8580
286	Y	A	-0.3661
287	A	A	0.0000
288	M	A	0.0000
289	H	A	0.0000
290	W	A	0.0000
291	V	A	0.0000
292	R	A	0.0000
293	Q	A	-0.7714
294	A	A	-1.3994
295	P	A	0.0000
296	G	A	-1.7038
297	K	A	-2.5248
298	G	A	-1.5823
299	L	A	0.0000
300	E	A	-1.0592
301	W	A	0.0000
302	V	A	0.0000
303	S	A	0.0000
304	G	A	0.0000
305	I	A	0.0000
306	S	A	0.0000
307	W	A	-0.2851
308	N	A	-1.2632
309	S	A	-0.7698
310	G	A	-0.5423
311	S	A	0.1445
312	I	A	1.0274
313	G	A	0.2392
314	Y	A	-0.6169
315	A	A	0.0000
316	D	A	-2.7039
317	S	A	-1.7978
318	V	A	0.0000
319	K	A	-2.5324
320	G	A	-1.6365
321	R	A	0.0000
322	F	A	0.0000
323	T	A	-0.6589
324	I	A	0.0000
325	S	A	-0.3724
326	R	A	-1.1980
327	D	A	-1.8646
328	N	A	-2.0289
329	A	A	-1.5080
330	K	A	-2.2780
331	N	A	-1.8658
332	S	A	-1.2403
333	L	A	0.0000
334	Y	A	-0.6408
335	L	A	0.0000
336	Q	A	-1.2306
337	M	A	0.0000
338	N	A	-1.8733
339	S	A	-1.7141
340	L	A	0.0000
341	R	A	-2.3499
342	A	A	-2.0027
343	E	A	-2.4521
344	D	A	0.0000
345	T	A	0.0000
346	A	A	0.0000
347	L	A	0.0664
348	Y	A	0.0000
349	Y	A	0.0000
350	C	A	0.0000
351	A	A	0.0000
352	K	A	0.0000
353	D	A	0.0000
354	S	A	-0.8773
355	R	A	-1.7927
356	G	A	-0.5771
357	Y	A	0.4846
358	G	A	-0.3248
359	D	A	-0.6194
360	Y	A	-0.3282
361	R	A	-1.8297
362	L	A	-0.9962
363	G	A	0.0000
364	G	A	0.0000
365	A	A	0.0000
366	Y	A	-0.0780
367	W	A	-0.1692
368	G	A	0.0000
369	Q	A	-1.2692
370	G	A	0.0000
371	T	A	0.0000
372	L	A	-0.3614
373	V	A	0.0000
374	T	A	0.0000
375	V	A	0.0000
376	S	A	-1.2386
377	S	A	-1.5188
378	G	A	-1.1966
379	G	A	-1.2525
380	G	A	-1.1556
381	G	A	-1.2086
382	S	A	-1.0340
383	G	A	-1.1914
384	G	A	-1.2044
385	G	A	-1.1919
386	G	A	-1.2082
387	S	A	-1.0360
388	G	A	-1.5204
389	G	A	-1.3825
390	G	A	-1.4702
391	G	A	-1.5150
392	S	A	-1.1915
393	E	A	-1.4692
394	I	A	0.0000
395	V	A	0.7451
396	M	A	0.0000
397	T	A	-0.6285
398	Q	A	0.0000
399	S	A	-0.6741
400	P	A	-0.3194
401	A	A	-0.5155
402	T	A	-0.6269
403	L	A	-0.3465
404	S	A	-0.6691
405	V	A	0.0000
406	S	A	-1.3593
407	P	A	-1.5439
408	G	A	-1.8769
409	E	A	-2.6561
410	R	A	-2.7932
411	A	A	0.0000
412	T	A	-0.6719
413	L	A	0.0000
414	S	A	-0.8806
415	C	A	0.0000
416	R	A	-2.1953
417	A	A	0.0000
418	S	A	-0.9597
419	Q	A	-1.7051
420	S	A	-1.4763
421	V	A	0.0000
422	S	A	-0.9122
423	S	A	-0.7824
424	N	A	-0.8482
425	L	A	0.0000
426	A	A	0.0000
427	W	A	0.0000
428	Y	A	0.0000
429	Q	A	0.0000
430	Q	A	0.0000
431	K	A	-1.5609
432	P	A	-0.9801
433	G	A	-1.8924
434	Q	A	-2.2266
435	A	A	-1.4904
436	P	A	0.0000
437	R	A	-1.6837
438	L	A	0.0000
439	L	A	0.0000
440	I	A	0.0000
441	Y	A	-0.1430
442	G	A	-0.4294
443	A	A	0.0000
444	S	A	-0.6345
445	T	A	-0.6602
446	R	A	-1.1660
447	A	A	0.0000
448	T	A	-0.5109
449	G	A	-0.5804
450	I	A	-0.5707
451	P	A	-0.3586
452	A	A	-0.3355
453	R	A	-0.5915
454	F	A	0.0000
455	S	A	-0.4878
456	G	A	0.0000
457	S	A	-0.7515
458	G	A	-1.2594
459	S	A	-1.2781
460	G	A	-1.3948
461	T	A	-1.9997
462	E	A	-2.7913
463	F	A	0.0000
464	T	A	-0.8579
465	L	A	0.0000
466	T	A	-0.5768
467	I	A	0.0000
468	S	A	-1.3583
469	S	A	-1.6900
470	L	A	0.0000
471	Q	A	-1.8675
472	S	A	-1.2428
473	E	A	-2.0738
474	D	A	0.0000
475	F	A	-0.6434
476	A	A	0.0000
477	V	A	-0.6410
478	Y	A	0.0000
479	Y	A	0.0000
480	C	A	0.0000
481	Q	A	0.0000
482	Q	A	0.0000
483	Y	A	0.0000
484	N	A	-1.1615
485	N	A	-1.4473
486	W	A	-0.6842
487	P	A	-1.0272
488	W	A	0.0000
489	T	A	-0.1874
490	F	A	0.0106
491	G	A	0.0000
492	Q	A	-1.4952
493	G	A	0.0000
494	T	A	0.0000
495	K	A	-1.3115
496	V	A	0.0000
497	E	A	-0.8001
498	I	A	0.3223
499	K	A	-1.4630
500	G	A	-1.3251
501	G	A	-1.4340
502	G	A	-2.1514
503	G	A	-2.2505
504	D	A	-3.1499
505	K	A	-3.0565
506	T	A	-1.8092
507	H	A	-1.5053
508	T	A	-0.5637
509	C	A	0.3270
510	P	A	-0.0413
511	P	A	0.1107
512	C	A	0.5126
513	P	A	-0.3715
514	A	A	-0.5279
515	P	A	-1.1190
516	E	A	-2.0282
517	A	A	-1.0897
518	A	A	-1.0308
519	G	A	-0.7849
520	G	A	-0.9490
521	P	A	0.0000
522	S	A	0.0000
523	V	A	0.0000
524	F	A	0.0000
525	L	A	0.0000
526	F	A	0.0000
527	P	A	-0.9010
528	P	A	0.0000
529	K	A	-2.8119
530	P	A	-1.6300
531	K	A	-1.3757
532	D	A	0.0000
533	T	A	0.0000
534	L	A	0.0000
535	Y	A	0.3299
536	I	A	1.3700
537	T	A	0.0847
538	R	A	-1.6297
539	E	A	-2.2684
540	P	A	0.0000
541	E	A	-1.7780
542	V	A	0.0000
543	T	A	-0.4818
544	C	A	0.0000
545	V	A	0.0000
546	V	A	0.0000
547	V	A	0.0000
548	D	A	0.0000
549	V	A	0.0000
550	S	A	-1.9990
551	H	A	-2.4961
552	E	A	-2.9414
553	D	A	-2.5518
554	P	A	-2.5845
555	E	A	-3.1249
556	V	A	-2.0277
557	K	A	-2.2567
558	F	A	-1.1667
559	N	A	-1.1052
560	W	A	0.0000
561	Y	A	-0.6082
562	V	A	-0.8767
563	D	A	-2.1172
564	G	A	-0.8689
565	V	A	0.5987
566	E	A	-0.6356
567	V	A	-0.5560
568	H	A	-1.8698
569	N	A	-2.1668
570	A	A	-1.8643
571	K	A	-2.3625
572	T	A	-1.7964
573	K	A	-1.9114
574	P	A	-2.0491
575	R	A	-3.0693
576	E	A	-2.9561
577	E	A	-2.7168
578	Q	A	-1.0701
579	Y	A	0.4313
580	N	A	0.0000
581	S	A	-0.4507
582	T	A	-1.1893
583	Y	A	0.0000
584	R	A	-1.8283
585	V	A	0.0000
586	V	A	0.0000
587	S	A	0.0000
588	V	A	0.0000
589	L	A	0.0000
590	T	A	-1.1678
591	V	A	0.0000
592	L	A	0.1524
593	H	A	-0.4916
594	Q	A	-1.2756
595	D	A	-1.5495
596	W	A	0.0000
597	L	A	-1.1631
598	N	A	-2.1088
599	G	A	-2.0825
600	K	A	-2.2096
601	E	A	-2.1823
602	Y	A	0.0000
603	K	A	-1.4620
604	C	A	0.0000
605	K	A	-1.3056
606	V	A	0.0000
607	S	A	-1.3875
608	N	A	0.0000
609	K	A	-2.4837
610	A	A	-1.4964
611	L	A	-0.6309
612	A	A	-0.5115
613	A	A	-0.2515
614	P	A	-0.7372
615	I	A	-0.3988
616	E	A	-1.6231
617	K	A	-1.1665
618	T	A	-1.1310
619	I	A	-0.5215
620	S	A	-1.3007
621	K	A	0.0000
622	A	A	-1.3156
623	K	A	-2.2847
624	G	A	-1.9505
625	Q	A	-1.9404
626	P	A	-1.7304
627	R	A	-2.0961
628	E	A	-2.5554
629	P	A	0.0000
630	Q	A	-1.2805
631	V	A	0.0000
632	Y	A	0.0000
633	T	A	-0.6585
634	L	A	0.0000
635	P	A	-0.3739
636	P	A	-0.5683
637	C	A	-0.9584
638	R	A	-1.9451
639	E	A	-1.4234
640	E	A	0.0000
641	M	A	-1.4162
642	T	A	-1.3475
643	K	A	-2.2219
644	N	A	-2.5238
645	Q	A	-2.2042
646	V	A	0.0000
647	S	A	0.0000
648	L	A	0.0000
649	W	A	0.0000
650	C	A	0.0000
651	L	A	0.0000
652	V	A	0.0000
653	K	A	-0.5579
654	G	A	-1.0440
655	F	A	0.0000
656	Y	A	-1.1307
657	P	A	0.0000
658	S	A	-0.4839
659	D	A	-1.5705
660	I	A	-0.7823
661	A	A	0.0000
662	V	A	0.0000
663	E	A	-1.2232
664	W	A	0.0000
665	E	A	-1.8475
666	S	A	0.0000
667	N	A	-1.9288
668	G	A	-1.7956
669	Q	A	-2.2840
670	P	A	-1.9323
671	E	A	-1.7955
672	N	A	-1.9911
673	N	A	-1.2688
674	Y	A	-0.8944
675	K	A	-0.9305
676	T	A	-0.3566
677	T	A	0.0000
678	P	A	-0.3978
679	P	A	-0.1139
680	V	A	0.0000
681	L	A	0.5855
682	D	A	-0.3898
683	S	A	-0.7192
684	D	A	-1.5794
685	G	A	-0.7684
686	S	A	0.0000
687	F	A	0.0742
688	F	A	0.0000
689	L	A	0.0000
690	Y	A	0.0000
691	S	A	0.0000
692	K	A	0.0000
693	L	A	0.0000
694	T	A	-0.7728
695	V	A	0.0000
696	D	A	-2.2417
697	K	A	-2.4565
698	S	A	-2.1392
699	R	A	-1.9808
700	W	A	0.0000
701	Q	A	-2.3517
702	Q	A	-2.1942
703	G	A	-1.3758
704	N	A	-1.1071
705	V	A	-0.2170
706	F	A	0.0000
707	S	A	0.0000
708	C	A	0.0000
709	S	A	0.0000
710	V	A	0.0000
711	M	A	0.0000
712	H	A	0.0000
713	E	A	-1.1894
714	A	A	-1.5757
715	L	A	0.0000
716	H	A	-1.8512
717	N	A	-1.6886
718	H	A	-1.2021
719	Y	A	-0.4873
720	T	A	-0.7445
721	Q	A	-1.0545
722	K	A	-0.9260
723	S	A	-0.5173
724	L	A	0.0000
725	S	A	-0.3205
726	L	A	-0.4484
727	S	A	-0.7116
728	P	A	-1.1012
729	G	A	-1.4218
730	K	A	-2.0276
1	D	B	-2.2660
2	V	B	0.0000
3	Q	B	-1.5928
4	L	B	0.0000
5	V	B	0.0000
6	E	B	0.0000
7	S	B	0.0000
8	G	B	0.0000
9	G	B	0.0000
10	G	B	0.0000
11	L	B	0.4509
12	V	B	0.0000
13	Q	B	-1.7889
14	A	B	-2.0614
15	G	B	-1.9017
16	G	B	-1.1660
17	S	B	-1.2286
18	L	B	0.0000
19	R	B	-1.1092
20	L	B	0.0000
21	S	B	-0.3410
22	C	B	0.0000
23	R	B	-0.8506
24	A	B	0.0000
25	S	B	-1.3012
26	G	B	-1.6240
27	H	B	-1.6865
28	T	B	-1.1687
29	F	B	0.0000
30	S	B	0.0000
31	D	B	-1.6254
32	T	B	0.0000
33	A	B	0.1188
34	V	B	0.0000
35	G	B	0.0000
36	W	B	0.0000
37	F	B	0.0000
38	R	B	0.0000
39	R	B	0.0000
40	V	B	-0.8219
41	P	B	-1.0173
42	G	B	-0.9834
43	K	B	-1.0855
44	E	B	0.0000
45	R	B	-0.5224
46	E	B	-0.6384
47	F	B	0.0000
48	V	B	0.0000
49	A	B	0.0000
50	T	B	0.2653
51	I	B	0.0000
52	A	B	-0.1443
53	G	B	-0.5438
54	S	B	-0.1907
55	G	B	0.1045
56	S	B	0.3812
57	V	B	0.9824
58	R	B	-0.2834
59	Y	B	-0.8054
60	S	B	-1.6355
61	E	B	-2.7030
62	S	B	-1.7622
63	V	B	0.0000
64	K	B	-2.6868
65	G	B	-1.9727
66	R	B	-2.1054
67	F	B	0.0000
68	T	B	-0.6432
69	I	B	0.0000
70	S	B	-0.1935
71	G	B	-0.7920
72	D	B	-1.4246
73	N	B	-1.7727
74	A	B	-1.5293
75	K	B	-2.2662
76	N	B	-1.6467
77	M	B	-1.0207
78	V	B	0.0000
79	Y	B	-0.3550
80	L	B	0.0000
81	Q	B	-0.9747
82	M	B	0.0000
83	N	B	-2.0447
84	R	B	-2.7064
85	L	B	0.0000
86	K	B	-2.3971
87	P	B	-1.8582
88	E	B	-2.2044
89	D	B	0.0000
90	T	B	-0.8016
91	G	B	0.0000
92	V	B	0.0000
93	Y	B	0.0000
94	Y	B	0.0000
95	C	B	0.0000
96	A	B	0.0000
97	A	B	0.0000
98	A	B	0.0000
99	V	B	0.3147
100	G	B	0.3850
101	I	B	1.8075
102	S	B	0.7286
103	Y	B	0.1142
104	D	B	-0.7663
105	Y	B	-0.5356
106	W	B	-0.2986
107	G	B	-0.6186
108	Q	B	-1.2921
109	G	B	0.0000
110	T	B	0.0000
111	Q	B	-0.5886
112	V	B	0.0000
113	T	B	-0.2861
114	V	B	0.0000
115	S	B	-1.3194
116	S	B	-2.0304
117	E	B	-2.7824
118	P	B	-2.2166
119	K	B	-2.7228
120	S	B	-2.1135
121	S	B	-2.2272
122	D	B	-3.1203
123	K	B	-2.9199
124	T	B	-1.7469
125	H	B	-1.4974
126	T	B	-0.5448
127	C	B	0.3239
128	P	B	-0.0399
129	P	B	0.1021
130	C	B	0.5063
131	P	B	-0.1471
132	A	B	-0.2105
133	P	B	-0.7890
134	E	B	-1.0428
135	A	B	-0.6216
136	A	B	-0.6955
137	G	B	-0.6528
138	G	B	-0.6491
139	P	B	0.0000
140	S	B	0.0000
141	V	B	0.0000
142	F	B	0.0000
143	L	B	0.0000
144	F	B	0.6019
145	P	B	-0.3209
146	P	B	0.0000
147	K	B	-2.3532
148	P	B	-1.3065
149	K	B	-1.1002
150	D	B	-1.4116
151	T	B	0.0000
152	L	B	0.0000
153	Y	B	0.0000
154	I	B	1.1856
155	T	B	-0.2199
156	R	B	-1.3451
157	E	B	-2.0067
158	P	B	0.0000
159	E	B	-1.6653
160	V	B	0.0000
161	T	B	0.0121
162	C	B	0.0000
163	V	B	0.0000
164	V	B	0.0000
165	V	B	-0.6664
166	D	B	-0.9593
167	V	B	0.0000
168	S	B	-1.3673
169	H	B	-1.6858
170	E	B	-2.6623
171	D	B	-1.8314
172	P	B	0.0000
173	E	B	0.0000
174	V	B	0.0000
175	K	B	-0.6122
176	F	B	-0.6330
177	N	B	-0.8973
178	W	B	0.0000
179	Y	B	-0.6126
180	V	B	-0.8559
181	D	B	-2.0950
182	G	B	-0.8688
183	V	B	0.5929
184	E	B	-0.6849
185	V	B	-0.5979
186	H	B	-1.9031
187	N	B	-2.1742
188	A	B	-1.8967
189	K	B	-2.3587
190	T	B	-1.4241
191	K	B	-1.7362
192	P	B	-1.3890
193	R	B	0.0000
194	E	B	-2.4195
195	E	B	-2.0802
196	Q	B	-0.9176
197	Y	B	0.3303
198	N	B	-0.5131
199	S	B	-0.9082
200	T	B	-1.1578
201	Y	B	0.0000
202	R	B	-1.3047
203	V	B	0.0000
204	V	B	-0.6307
205	S	B	0.0000
206	V	B	0.0000
207	L	B	0.0000
208	T	B	-1.1482
209	V	B	0.0000
210	L	B	0.3458
211	H	B	-0.3304
212	Q	B	-1.0449
213	D	B	-1.3922
214	W	B	0.0000
215	L	B	-0.9044
216	N	B	-2.0176
217	G	B	-2.0699
218	K	B	-2.1427
219	E	B	-2.0741
220	Y	B	0.0000
221	K	B	-1.3666
222	C	B	0.0000
223	K	B	-1.1864
224	V	B	0.0000
225	S	B	-0.7694
226	N	B	0.0000
227	K	B	-1.5287
228	A	B	-0.9369
229	L	B	0.0000
230	A	B	-0.5284
231	A	B	-0.6113
232	P	B	-0.9058
233	I	B	0.0000
234	E	B	-1.9029
235	K	B	0.0000
236	T	B	-1.2857
237	I	B	0.0000
238	S	B	-1.3368
239	K	B	-1.3341
240	A	B	-1.0825
241	K	B	-2.3883
242	G	B	-1.9853
243	Q	B	-2.1377
244	P	B	-1.7717
245	R	B	-2.0579
246	E	B	-2.5950
247	P	B	0.0000
248	Q	B	-0.9481
249	V	B	0.0000
250	C	B	0.0000
251	T	B	0.0000
252	L	B	0.0000
253	P	B	-0.3721
254	P	B	-0.6930
255	S	B	-1.2866
256	R	B	-2.3195
257	E	B	-2.0730
258	E	B	0.0000
259	M	B	-1.5312
260	T	B	-1.3302
261	K	B	-1.8883
262	N	B	-2.2664
263	Q	B	-1.8961
264	V	B	0.0000
265	S	B	0.0000
266	L	B	0.0000
267	S	B	0.0000
268	C	B	0.0000
269	A	B	0.0000
270	V	B	0.0000
271	K	B	-0.6270
272	G	B	-1.1264
273	F	B	0.0000
274	Y	B	-1.1702
275	P	B	0.0000
276	S	B	0.0000
277	D	B	-1.0577
278	I	B	-0.5059
279	A	B	0.0000
280	V	B	0.0000
281	E	B	-0.2012
282	W	B	0.0000
283	E	B	0.0000
284	S	B	0.0000
285	N	B	-0.7118
286	G	B	0.0000
287	Q	B	-0.1383
288	P	B	0.0000
289	E	B	0.0000
290	N	B	0.0000
291	N	B	0.0758
292	Y	B	-0.2215
293	K	B	-0.3675
294	T	B	-0.1637
295	T	B	0.0000
296	P	B	-0.3947
297	P	B	-0.0635
298	V	B	0.0000
299	L	B	-0.0289
300	D	B	-0.9065
301	S	B	-1.2913
302	D	B	-2.0229
303	G	B	-1.1099
304	S	B	0.0000
305	F	B	0.0000
306	F	B	0.0000
307	L	B	0.0000
308	V	B	0.0000
309	S	B	0.0000
310	K	B	0.0000
311	L	B	0.0000
312	T	B	-0.2529
313	V	B	0.0000
314	D	B	-2.2913
315	K	B	-2.3913
316	S	B	-2.1464
317	R	B	-1.9680
318	W	B	0.0000
319	Q	B	-2.3213
320	Q	B	-2.1132
321	G	B	-1.2100
322	N	B	-0.7822
323	V	B	0.2537
324	F	B	0.0000
325	S	B	0.0000
326	C	B	0.0000
327	S	B	0.0000
328	V	B	0.0000
329	M	B	0.0000
330	H	B	0.0000
331	E	B	-1.1488
332	A	B	-1.6038
333	L	B	0.0000
334	H	B	-1.9414
335	N	B	-1.8668
336	H	B	-1.4294
337	Y	B	-0.7151
338	T	B	-0.5165
339	Q	B	0.0000
340	K	B	-0.5398
341	S	B	-0.3644
342	L	B	0.0000
343	S	B	-0.1655
344	L	B	-0.2808
345	S	B	-0.6493
346	P	B	-1.0564
347	G	B	-1.4034
348	K	B	-2.0273

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Automated mutations analysis - evolutionary conserved mutations

In the automated mutations mode, the server selects aggregation prone resides and each selected residue is mutated based off an evolutionary approach. The table below shows 2 best scored mutants for each mutated residue. Protein variants are ordered according to the mutation effect they had on protein stability (energetic effect) together with the difference in the average per-residue aggregation score between the wild type and the mutant (in the table green values indicate a positive change, grey are neutral, and orange/red mean destabilizing or more aggregation prone mutants).
Summary for all the mutants can be found in this CSV file .

Mutant	Energetic effect	Score comparison
IM154B	-0.5711	-0.0019	View	CSV	PDB
LM116A	-0.2269	-0.0025	View	CSV	PDB
IM536A	-0.5463	-0.0011	View	CSV	PDB
IL154B	-0.553	-0.0009	View	CSV	PDB
LM11A	-0.0365	-0.0015	View	CSV	PDB
IL536A	-0.504	-0.0004	View	CSV	PDB
IM101B	0.0742	-0.0034	View	CSV	PDB
IT76A	0.7805	-0.0058	View	CSV	PDB
IM76A	0.3543	-0.0025	View	CSV	PDB
IT101B	0.9879	-0.0057	View	CSV	PDB

Project name: F2B_K0_H0

Status: done

Started: 2026-05-29 06:46:44

Automated mutations analysis - evolutionary conserved mutations

Mutant

Energetic effect

Score comparison